##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233055_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35889287
Sequences flagged as poor quality	0
Sequence length	101
%GC	26
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.373541803714296	34.0	31.0	34.0	31.0	34.0
2	32.68204514622985	34.0	33.0	34.0	31.0	34.0
3	32.71549365135061	34.0	33.0	34.0	31.0	34.0
4	36.00995330444988	37.0	37.0	37.0	35.0	37.0
5	36.00449769314169	37.0	37.0	37.0	35.0	37.0
6	36.03160829023992	37.0	37.0	37.0	35.0	37.0
7	35.95255202478667	37.0	37.0	37.0	35.0	37.0
8	35.75724076101038	37.0	37.0	37.0	35.0	37.0
9	37.52549165994855	39.0	39.0	39.0	35.0	39.0
10-11	37.58989172730013	39.0	39.0	39.0	36.0	39.0
12-13	37.71014535618944	39.0	39.0	39.0	37.0	39.0
14-15	39.25195251162276	41.0	40.0	41.0	37.5	41.0
16-17	39.05308138331085	41.0	39.5	41.0	37.0	41.0
18-19	38.419870085465895	41.0	38.5	41.0	35.5	41.0
20-21	37.9812915202244	41.0	37.5	41.0	35.0	41.0
22-23	37.61592403605009	40.0	36.5	41.0	35.0	41.0
24-25	37.033042353279406	40.5	35.0	41.0	33.0	41.0
26-27	36.62364905716851	40.5	35.0	41.0	32.0	41.0
28-29	35.99277688353073	40.0	35.0	41.0	27.5	41.0
30-31	35.339914610173224	40.0	35.0	41.0	21.5	41.0
32-33	34.97615281406956	40.0	35.0	41.0	17.0	41.0
34-35	34.73004851001916	40.0	35.0	41.0	14.0	41.0
36-37	34.52837722855848	40.0	35.0	41.0	9.5	41.0
38-39	34.334231953396014	40.0	35.0	41.0	8.5	41.0
40-41	34.173071019772564	40.0	35.0	41.0	7.0	41.0
42-43	33.983600955906425	39.5	35.0	41.0	6.0	41.0
44-45	33.93001577323061	39.0	35.0	41.0	3.5	41.0
46-47	33.61863568089274	39.0	35.0	41.0	2.0	41.0
48-49	33.52074118942514	39.0	35.0	41.0	2.0	41.0
50-51	32.849545004892406	37.5	34.5	40.5	2.0	41.0
52-53	32.76042852286255	37.0	35.0	40.5	2.0	41.0
54-55	32.87586896056197	37.0	35.0	41.0	2.0	41.0
56-57	32.72928860358803	36.0	35.0	41.0	2.0	41.0
58-59	32.426677283948266	36.0	35.0	41.0	2.0	41.0
60-61	32.23372612557057	36.0	35.0	41.0	2.0	41.0
62-63	31.96269092779692	35.0	34.5	41.0	2.0	41.0
64-65	31.505499593792432	35.0	33.5	40.0	2.0	41.0
66-67	30.32187085522206	35.0	32.0	40.0	2.0	41.0
68-69	29.601676065618133	35.0	30.5	40.0	2.0	41.0
70-71	29.142404681931964	35.0	29.0	39.0	2.0	41.0
72-73	28.642819652560945	35.0	26.5	39.0	2.0	41.0
74-75	28.16719065775812	35.0	24.0	38.0	2.0	41.0
76-77	27.69096897912739	35.0	19.0	37.0	2.0	40.0
78-79	27.28643604705772	35.0	14.0	37.0	2.0	39.5
80-81	26.954076407257688	35.0	7.5	36.0	2.0	39.0
82-83	26.57404223438599	35.0	2.0	36.0	2.0	39.0
84-85	26.150664988134203	35.0	2.0	35.5	2.0	37.5
86-87	25.70606872184449	35.0	2.0	35.0	2.0	37.0
88-89	25.18372206725645	35.0	2.0	35.0	2.0	37.0
90-91	24.71150148510891	34.5	2.0	35.0	2.0	36.0
92-93	24.169790040688184	34.0	2.0	35.0	2.0	36.0
94-95	23.675519535955118	34.0	2.0	35.0	2.0	35.0
96-97	22.989872437421226	33.0	2.0	35.0	2.0	35.0
98-99	22.36415174533838	33.0	2.0	35.0	2.0	35.0
100-101	21.084726481192007	30.5	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0051255368320894945
1101	2	0.06776099113118761
1101	3	0.17245319864412068
1101	4	0.016562602727425713
1101	5	-0.0294923335617554
1101	6	-0.03355659619325024
1101	7	-0.08520878922291786
1101	8	-0.10433883252165543
1101	9	-0.09640664463176307
1101	10-11	-0.07396061875459026
1101	12-13	-0.03610866164738269
1101	14-15	0.030244845183112545
1101	16-17	0.032295069994965786
1101	18-19	0.050726982690214584
1101	20-21	0.04713217772480505
1101	22-23	-0.03731346829091109
1101	24-25	0.0039671041487210346
1101	26-27	-0.048678382575182866
1101	28-29	-0.03625453859059746
1101	30-31	-0.004427848268193202
1101	32-33	-0.037577546648257965
1101	34-35	-0.08662112839574831
1101	36-37	-0.0836374711739225
1101	38-39	-0.09775520257115744
1101	40-41	-0.15675884655063044
1101	42-43	-0.08930213896943684
1101	44-45	-0.06792388347231082
1101	46-47	-0.07432986971776501
1101	48-49	-0.09985712247775069
1101	50-51	-0.20851746664622084
1101	52-53	-0.16636843588135974
1101	54-55	-0.0941247137393404
1101	56-57	-0.1313559390510335
1101	58-59	-0.11458724412405985
1101	60-61	-0.19730064819280813
1101	62-63	-0.18598031173490526
1101	64-65	-0.1839761324618827
1101	66-67	-0.12714471939354866
1101	68-69	0.1317865713896822
1101	70-71	0.14734045817043295
1101	72-73	0.2550202053746524
1101	74-75	0.18356044736888322
1101	76-77	0.17758449910421703
1101	78-79	0.20654187325515494
1101	80-81	0.17642888273949353
1101	82-83	0.209627925873356
1101	84-85	0.19330372887892366
1101	86-87	0.181023984227771
1101	88-89	0.19047099001248569
1101	90-91	0.24049378311936565
1101	92-93	0.31813976939238486
1101	94-95	0.3325795356931778
1101	96-97	0.3442933952210545
1101	98-99	0.37638726349579343
1101	100-101	0.4191700325292018
1102	1	0.04841656298807351
1102	2	0.08342025326768976
1102	3	0.17463073896453096
1102	4	0.048385684531289996
1102	5	0.025564779830105522
1102	6	0.005351577879551428
1102	7	-0.031126304127802484
1102	8	-0.030527498056081015
1102	9	-0.008606685497582589
1102	10-11	0.011635846195680699
1102	12-13	0.002855906420286658
1102	14-15	0.061446754649900015
1102	16-17	0.08679494831483225
1102	18-19	0.15013852940685268
1102	20-21	0.13854755873168756
1102	22-23	0.08040353237466746
1102	24-25	0.1132026918730773
1102	26-27	0.009537843656922007
1102	28-29	0.04712017282027148
1102	30-31	0.09307422194369508
1102	32-33	0.10660168052158525
1102	34-35	0.10103103814177672
1102	36-37	0.1055145226559091
1102	38-39	0.06982269286164922
1102	40-41	0.07473297246740884
1102	42-43	0.11873135732133733
1102	44-45	0.09902105351920198
1102	46-47	0.06892495737044158
1102	48-49	0.05553090598919397
1102	50-51	0.07424458788900523
1102	52-53	0.08216240063525504
1102	54-55	0.09734865302913676
1102	56-57	0.0956032983530406
1102	58-59	0.09324264155056738
1102	60-61	0.014031729210309152
1102	62-63	0.025151361270744133
1102	64-65	0.05246648298410861
1102	66-67	0.09899518461472567
1102	68-69	0.3046278672857703
1102	70-71	0.3252322970606656
1102	72-73	0.38102758562955685
1102	74-75	0.3679233924854195
1102	76-77	0.3107425693299959
1102	78-79	0.33651570654145146
1102	80-81	0.29534721447121726
1102	82-83	0.2678236859494625
1102	84-85	0.2721988627635028
1102	86-87	0.26629974324632144
1102	88-89	0.3083300316050064
1102	90-91	0.32868112135615135
1102	92-93	0.3981267744937611
1102	94-95	0.4209377822057583
1102	96-97	0.44371918826797696
1102	98-99	0.5300269568588476
1102	100-101	0.5649669811877942
1103	1	0.08579625610521191
1103	2	0.07610083999903594
1103	3	0.18010767981878217
1103	4	0.08541084406199673
1103	5	0.05759572004181024
1103	6	0.04708432294914644
1103	7	0.040793196486291095
1103	8	0.006024480788305198
1103	9	0.025092315654411834
1103	10-11	0.009659550155838303
1103	12-13	0.03228816507714072
1103	14-15	0.08784886698821737
1103	16-17	0.07015832454083437
1103	18-19	0.0849123299782093
1103	20-21	0.14566062337403451
1103	22-23	0.13303442020030332
1103	24-25	0.12193526855707404
1103	26-27	0.062101500985356495
1103	28-29	0.10581046182684872
1103	30-31	0.12705559553822354
1103	32-33	0.0956073414627383
1103	34-35	0.1145192410889706
1103	36-37	0.0963827238125674
1103	38-39	0.05932689223102727
1103	40-41	0.061948418470201716
1103	42-43	0.08836174467505487
1103	44-45	0.09315690482586803
1103	46-47	0.12250097550406736
1103	48-49	0.12710987245007033
1103	50-51	0.1140317392256307
1103	52-53	0.11644822357409623
1103	54-55	0.062252612371331395
1103	56-57	0.08666142020840084
1103	58-59	0.10848054055210099
1103	60-61	0.10986517796165884
1103	62-63	0.05768911723330916
1103	64-65	0.11462990056085332
1103	66-67	0.11633491787609174
1103	68-69	0.26571631632982573
1103	70-71	0.2771378205970372
1103	72-73	0.37436612499364585
1103	74-75	0.3751133574077983
1103	76-77	0.2881144946337848
1103	78-79	0.3395725518274695
1103	80-81	0.3105033314340133
1103	82-83	0.2602566644197175
1103	84-85	0.2175966405722889
1103	86-87	0.23368265446791625
1103	88-89	0.3117362571622948
1103	90-91	0.2949728778978731
1103	92-93	0.38862061818133853
1103	94-95	0.3940218500978325
1103	96-97	0.4381215535368277
1103	98-99	0.4913575286712657
1103	100-101	0.47271587702741513
1104	1	0.06806996412243649
1104	2	0.0853669722550876
1104	3	0.16196509405149584
1104	4	0.08327763594537174
1104	5	0.027972826353668268
1104	6	0.0020809510955857036
1104	7	-0.004944819147070234
1104	8	-0.015895340438248695
1104	9	-0.05358053353646142
1104	10-11	-0.027354820402713642
1104	12-13	-0.008453820979532622
1104	14-15	0.0016204725285291488
1104	16-17	0.021226939621584506
1104	18-19	0.04469809827969584
1104	20-21	0.04215449988694786
1104	22-23	0.03562430245076342
1104	24-25	0.027697252433391384
1104	26-27	0.016765924138915977
1104	28-29	0.028520821784312034
1104	30-31	0.042772750953744776
1104	32-33	0.02128157018805865
1104	34-35	-4.13379416855264E-4
1104	36-37	0.0024956329418444057
1104	38-39	-0.02032107806443406
1104	40-41	-0.03851737868993865
1104	42-43	0.02244996309547531
1104	44-45	0.03226212115381344
1104	46-47	-0.003193404567312541
1104	48-49	0.06477805000567116
1104	50-51	0.056915451363757086
1104	52-53	0.06146268749056105
1104	54-55	0.05077090651005989
1104	56-57	0.06137088379924194
1104	58-59	0.06218664004224905
1104	60-61	0.05092659837846014
1104	62-63	0.0349790690567815
1104	64-65	0.04874591660390237
1104	66-67	0.09431876527197147
1104	68-69	0.18367543960453503
1104	70-71	0.19076518325709912
1104	72-73	0.29911679932303414
1104	74-75	0.27343330060184456
1104	76-77	0.1929470272627256
1104	78-79	0.18755659053259066
1104	80-81	0.19671346612642893
1104	82-83	0.16033159203220393
1104	84-85	0.1399950914294621
1104	86-87	0.1706292880499376
1104	88-89	0.20093582557294098
1104	90-91	0.21096364987990412
1104	92-93	0.2646652510674059
1104	94-95	0.2619396162263392
1104	96-97	0.2650069496225349
1104	98-99	0.28430513735478513
1104	100-101	0.21104137669287582
1105	1	0.09291167873328732
1105	2	0.09428950981404682
1105	3	0.15322181890373088
1105	4	0.054820601883243114
1105	5	0.03866650731644938
1105	6	0.015063108395942493
1105	7	0.011039761391195668
1105	8	0.10041760571714775
1105	9	0.05183695220623008
1105	10-11	0.06970434346641241
1105	12-13	0.02219005846157529
1105	14-15	0.015548647351174338
1105	16-17	0.027614256036791573
1105	18-19	0.025585147627353422
1105	20-21	-0.0018793961589125274
1105	22-23	0.00148877436587469
1105	24-25	-3.0040493317784467E-4
1105	26-27	0.018735903923975172
1105	28-29	0.026294331063297705
1105	30-31	0.012113088785412174
1105	32-33	0.0060409482436298845
1105	34-35	-0.008453562124969949
1105	36-37	0.013875843612893846
1105	38-39	0.01868888966620119
1105	40-41	0.005118143232813566
1105	42-43	-0.008872255918383587
1105	44-45	0.02566225994508642
1105	46-47	0.0512625562517286
1105	48-49	0.051816000091619685
1105	50-51	0.012977660098634658
1105	52-53	0.011613369851204425
1105	54-55	0.03999635850146177
1105	56-57	0.028977316489147142
1105	58-59	0.012333185900281762
1105	60-61	0.07175047978862636
1105	62-63	0.06495346128327384
1105	64-65	0.12867950195284905
1105	66-67	0.11888026251663675
1105	68-69	0.1447748196368721
1105	70-71	0.15551099195243978
1105	72-73	0.276375098815695
1105	74-75	0.23194050834592872
1105	76-77	0.15888784477199636
1105	78-79	0.20863482551032675
1105	80-81	0.14866528796594736
1105	82-83	0.09937499425056373
1105	84-85	0.09763268383935753
1105	86-87	0.10695292142723645
1105	88-89	0.11995764910216877
1105	90-91	0.1692702083991655
1105	92-93	0.2714895684405505
1105	94-95	0.2554429064790149
1105	96-97	0.29858976859926045
1105	98-99	0.3360260074419088
1105	100-101	0.3176557717401849
1106	1	0.05852272681320159
1106	2	0.06686307025027105
1106	3	0.14689645354694392
1106	4	0.07262366375488938
1106	5	0.030543625861277235
1106	6	0.0011529918719119792
1106	7	-6.839410954881942E-4
1106	8	0.09397374900562738
1106	9	0.05901409214325071
1106	10-11	0.06435420593521002
1106	12-13	0.022131802616961238
1106	14-15	-1.5475325425740039E-4
1106	16-17	0.020181637735745994
1106	18-19	0.015146178320790682
1106	20-21	0.03723557098275165
1106	22-23	0.007689301585024566
1106	24-25	-0.03778769633610324
1106	26-27	-0.05205811715929798
1106	28-29	-0.04992682778876656
1106	30-31	-0.040086565484983794
1106	32-33	-0.049565935833392416
1106	34-35	-0.019783269229812106
1106	36-37	0.01870628664181595
1106	38-39	-3.890073592529575E-4
1106	40-41	-0.033559831499509585
1106	42-43	-0.07180976056466193
1106	44-45	-0.0649363201882025
1106	46-47	-0.015516901390313365
1106	48-49	-0.042617756327921086
1106	50-51	-0.07921371569076996
1106	52-53	-0.1257112396619462
1106	54-55	-0.11431932921831844
1106	56-57	-0.12891470143216566
1106	58-59	-0.10296996790893331
1106	60-61	-0.03824540895940487
1106	62-63	-0.05493967223980434
1106	64-65	-0.015438086582832966
1106	66-67	5.418091604205699E-4
1106	68-69	-0.011863622244025152
1106	70-71	0.018668778832484634
1106	72-73	0.19760361365836232
1106	74-75	0.12899099836579353
1106	76-77	0.06837752083504967
1106	78-79	0.09760193822190288
1106	80-81	0.029133272602095417
1106	82-83	0.05834080970287303
1106	84-85	0.07873795226232261
1106	86-87	0.03561622016897914
1106	88-89	0.014819654825085848
1106	90-91	0.054405593048162615
1106	92-93	0.12174032005194846
1106	94-95	0.12101036732181214
1106	96-97	0.12115816618110031
1106	98-99	0.1502719167601505
1106	100-101	0.07925482518463411
1107	1	-0.008664648967261712
1107	2	-0.028392710934838306
1107	3	-6.384530956111689E-4
1107	4	-0.06454848234455568
1107	5	-0.05741988905232631
1107	6	-0.12587813980419327
1107	7	-0.07579228780184621
1107	8	0.06436608088824869
1107	9	0.01772552335111044
1107	10-11	-0.04999994371991079
1107	12-13	-0.09261715937126525
1107	14-15	-0.15309687443684794
1107	16-17	-0.16186234909613262
1107	18-19	-0.19766295588924265
1107	20-21	-0.2418860796787783
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2308	9	-0.16078045171390443
2308	10-11	-0.24988275618348865
2308	12-13	-0.31610422874792476
2308	14-15	-0.39884272575597635
2308	16-17	-0.41383361111532935
2308	18-19	-0.5052983167008591
2308	20-21	-0.5248749466328491
2308	22-23	-0.5695090150215449
2308	24-25	-0.4947501363105218
2308	26-27	-0.4403341149117068
2308	28-29	-0.5473347999790121
2308	30-31	-0.5728514416212747
2308	32-33	-0.554757342843935
2308	34-35	-0.5182569326814743
2308	36-37	-0.45642749879960576
2308	38-39	-0.4235787165213196
2308	40-41	-0.40705466019826275
2308	42-43	-0.4540041958547789
2308	44-45	-0.5577478355141565
2308	46-47	-0.5454116872555659
2308	48-49	-0.7871614131026234
2308	50-51	-0.6096113683037458
2308	52-53	-0.4829503514485154
2308	54-55	-0.47371376396002773
2308	56-57	-0.5509639931403854
2308	58-59	-0.4928241187122566
2308	60-61	-0.45501841439321566
2308	62-63	-0.4435886184716793
2308	64-65	-0.522038892350789
2308	66-67	-0.2243705938789411
2308	68-69	-0.4363427420992103
2308	70-71	-0.5433190750569103
2308	72-73	-0.3958002265175544
2308	74-75	-0.4510055649333893
2308	76-77	-0.5525202482117493
2308	78-79	-0.5757541469805751
2308	80-81	-0.6369984185041453
2308	82-83	-0.7351993139216368
2308	84-85	-0.7653719225663664
2308	86-87	-0.810330230387649
2308	88-89	-0.9040783754862503
2308	90-91	-0.9930990798837591
2308	92-93	-1.0911802788022342
2308	94-95	-1.2398528461367988
2308	96-97	-1.4748399275570385
2308	98-99	-1.7587454475811342
2308	100-101	-2.0436172559613084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	239819.0
3	65126.0
4	43608.0
5	42116.0
6	66158.0
7	109096.0
8	191124.0
9	373120.0
10	606702.0
11	640043.0
12	522681.0
13	415660.0
14	323109.0
15	282640.0
16	263726.0
17	259853.0
18	277745.0
19	284536.0
20	309134.0
21	346267.0
22	394548.0
23	439301.0
24	631166.0
25	1231805.0
26	520086.0
27	550337.0
28	456079.0
29	449199.0
30	511389.0
31	585771.0
32	683930.0
33	813592.0
34	1091060.0
35	3351186.0
36	3454682.0
37	4198894.0
38	7182473.0
39	3669223.0
40	12303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.20319193510123	22.32497054511781	10.23556283596986	38.236274683811104
2	19.189034234542895	19.44222338089772	46.36334408340498	15.005398301154404
3	18.040179235293202	26.017054972015913	37.04408908335598	18.898676709334904
4	17.117499531224624	29.4305999481849	37.19805018472736	16.25385033586311
5	19.270025356537936	28.784801038825314	34.95342089954858	16.991752705088167
6	19.519528207193197	32.87690653907701	35.44619915284943	12.157366100880367
7	14.716049149635607	20.851400252633614	53.03919848300298	11.393352114727803
8	10.24243060289875	28.691912716917777	45.85061751660259	15.21503916358088
9	15.083512563096377	26.368876656316136	45.40127422912206	13.146336551465431
10-11	16.284858790969135	34.99649472708959	38.253969593070444	10.464676888870837
12-13	16.2613043370157	26.637549382048665	42.850475305274024	14.250670975661608
14-15	15.426626008185226	28.228067405106138	45.03803113577828	11.307275450930353
16-17	17.416085809282812	27.472446518267386	41.634143421893164	13.477324250556642
18-19	16.01786679633149	28.339045384416305	44.25106932332011	11.3920184959321
20-21	15.074612650720162	31.073317652584098	42.94659186100685	10.90547783568889
22-23	19.36893203680018	28.191972877611228	41.419826839194165	11.01926824639443
24-25	15.960095994719929	33.05102901483302	40.00603564635974	10.982839344087308
26-27	15.28837417558172	34.30676790936028	39.64385487891696	10.76100303614104
28-29	16.048938042495056	34.43883100754498	38.645829289769054	10.866401660190913
30-31	17.07894265312052	31.31148958407457	40.97078683767247	10.638780925132439
32-33	15.199253172387026	33.16552818847308	40.79505182420689	10.84016681493301
34-35	16.249632044713294	34.95498024188225	38.08208633047385	10.713301382930611
36-37	12.144747952301724	35.43212932188526	41.03852597719091	11.384596748622101
38-39	12.88339316152225	32.48919287364332	40.9941372831884	13.633276681646034
40-41	15.455286352167882	31.84687486639265	39.291735024729064	13.406103756710408
42-43	14.864274433307076	32.064101476398974	41.942779577246505	11.128844513047444
44-45	18.539958267004067	31.23591519770943	39.19292733630561	11.031199198980895
46-47	12.825165450450838	32.61537875132113	40.61575248218816	13.943703316039874
48-49	14.100265764845856	32.27048908956199	39.06299804771221	14.566247097879945
50-51	15.694012531871133	31.818635269776273	39.09803578355087	13.389316414801723
52-53	12.701501118135065	35.72575749322766	40.98631242786034	10.586428960776933
54-55	12.948570483453176	34.330318395353444	38.99709464747001	13.724016473723378
56-57	12.29062399609925	35.76693498028447	40.30337574909315	11.63906527452313
58-59	12.265810359803076	37.474236289564125	38.62676392188045	11.633189428752349
60-61	11.191214922313021	40.1690256430497	37.2429316141301	11.39682782050718
62-63	11.649548249996178	41.22369082032581	36.225394594576365	10.901366335101656
64-65	11.649844665634385	40.474375885498986	37.07018655873202	10.805592890134614
66-67	11.946887563395894	42.242297103419105	35.40106226490037	10.409753068284633
68-69	12.961780753847258	41.266662159817066	35.43156546350044	10.339991622835232
70-71	11.357588335769103	42.628748933890606	35.15186082249421	10.861801907846084
72-73	12.43810579712672	41.14740670989255	35.32578999772147	11.088697495259263
74-75	12.772253927286616	40.69658426431656	35.502392264473784	11.028769543923039
76-77	13.215789256917976	39.50588587997183	36.18290469314265	11.095420169967541
78-79	12.71474538722348	40.34808080177409	35.912488808811574	11.024685002190852
80-81	13.758542526687636	40.14143101625742	35.157067385231045	10.9429590718239
82-83	13.401754858214968	38.835164522662424	35.816461564261374	11.946619054861241
84-85	13.405547131795748	38.82245911843391	35.637612975666805	12.134380774103533
86-87	15.309431723511638	38.8268011570524	34.58872773612166	11.2750393833143
88-89	14.828443196208251	38.605503948202625	35.2597900638505	11.306262791738629
90-91	14.690856931232524	38.696916533117545	34.94338170145496	11.668844834194969
92-93	15.243772846381399	38.46440338232181	33.965353530827905	12.326470240468886
94-95	14.778447808546776	38.66629482061716	33.32368680478828	13.231570566047784
96-97	15.345636133437493	38.74444263002568	34.20337144904548	11.706549787491353
98-99	15.256763646074148	39.09030070758457	33.6482917841552	12.00464386218609
100-101	16.42867075876254	38.88291821332085	32.04149653749451	12.646914490422102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	883394.0
1	825722.0
2	632232.5
3	555083.5
4	685169.0
5	817403.0
6	780727.0
7	654830.5
8	612888.5
9	572441.5
10	531623.0
11	497544.0
12	486099.0
13	471436.5
14	528927.5
15	536464.0
16	439128.0
17	410642.5
18	409262.0
19	417606.5
20	438459.0
21	470506.5
22	510952.5
23	558563.5
24	615683.0
25	698416.5
26	783447.0
27	851192.0
28	895893.0
29	925526.5
30	951067.0
31	946002.0
32	932164.0
33	928945.5
34	932377.0
35	924984.5
36	930941.0
37	947295.0
38	944743.5
39	927482.0
40	914981.5
41	896999.5
42	819597.0
43	739559.0
44	681035.0
45	614555.5
46	543259.5
47	472909.0
48	405016.5
49	337025.5
50	274411.5
51	218553.5
52	175606.5
53	153577.5
54	191634.0
55	332588.0
56	300185.5
57	132802.0
58	78464.5
59	51974.0
60	35421.0
61	25106.0
62	18470.5
63	13655.0
64	10049.0
65	7272.5
66	5319.5
67	3886.5
68	2870.5
69	2205.5
70	1693.0
71	1393.0
72	1174.0
73	937.5
74	751.5
75	581.5
76	421.5
77	345.5
78	306.5
79	229.5
80	189.0
81	152.5
82	107.0
83	89.5
84	70.0
85	52.5
86	44.5
87	38.5
88	32.5
89	23.5
90	16.5
91	17.0
92	13.5
93	10.0
94	13.5
95	10.5
96	4.5
97	4.0
98	2.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20241137696605677
2	0.19674673392090516
3	0.2049998931436002
4	0.17245536251528207
5	0.17890296901133756
6	0.2191517485426779
7	0.23205810692199041
8	0.225747031419153
9	0.25928628785520313
10-11	0.2557559864591347
12-13	0.26302277891449893
14-15	0.2689075433568797
16-17	0.2800975121071645
18-19	0.288912120210134
20-21	0.280915304893073
22-23	0.28880066633811924
24-25	0.28393431165127353
26-27	0.25782345578500904
28-29	0.22668881663767798
30-31	0.2284887966707168
32-33	0.27575359744538813
34-35	0.2936461234239621
36-37	0.28548769999247964
38-39	0.28716786711310255
40-41	0.28826708092584846
42-43	0.29159397900548983
44-45	0.29360154187515625
46-47	0.2902746437955148
48-49	0.05882535364940518
50-51	0.06401631773849395
52-53	0.045030150640774785
54-55	0.04942004002475725
56-57	0.04224241066700489
58-59	0.03419683428093737
60-61	0.03659309252925532
62-63	0.04046472140836902
64-65	0.04107075183744943
66-67	0.046243604672335785
68-69	0.05004836122824061
70-71	0.042551695161845926
72-73	0.0487345987118663
74-75	0.04125047120607327
76-77	0.0420306483101768
78-79	0.035796197344349585
80-81	0.05000795919963526
82-83	0.05971698462552349
84-85	0.05897303002982478
86-87	0.061132448800111296
88-89	0.061699470373986535
90-91	0.06657836362143388
92-93	0.060564034052835884
94-95	0.06461398912717324
96-97	0.062068661325035517
98-99	0.0557422609147961
100-101	0.057017014575965246
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5889287E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.01727683686004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93377874686773	30.008473183991303
2	11.633077538062741	8.845158584583789
3	3.4389388205855664	3.9221726750167925
4	1.633045695298025	2.483358011415504
5	0.9295142002119576	1.7668799336625272
6	0.5963472059201331	1.360289809101221
7	0.4171907636488799	1.110231972879436
8	0.3158476176177525	0.9606133053789446
9	0.24422658812806633	0.8356346830607844
>10	1.5752350781993452	11.42204645751914
>50	0.15222971930269083	4.012638633023696
>100	0.11154788484946573	8.303159058981446
>500	0.0107087266806385	2.81225392760273
>1k	0.007344797437338783	5.32620899127397
>5k	5.668882768110699E-4	1.4452717131015167
>10k+	3.9972891290762164E-4	15.385609059407338
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1332429	3.712609280869804	Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	815526	2.272338260718303	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	574755	1.601466755246489	No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	352872	0.9832237681400581	Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	334565	0.9322141172656898	Illumina Single End PCR Primer 1 (100% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	309604	0.8626641147816617	Illumina Single End PCR Primer 1 (100% over 43bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257949	0.7187353708085648	No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT	120697	0.33630369976422214	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99352	0.27682912731033077	No Hit
GCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88919	0.2477591711420737	No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC	83750	0.2333565445309627	Illumina Single End PCR Primer 1 (100% over 47bp)
GCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	83325	0.23217234714080556	Illumina Single End PCR Primer 1 (100% over 40bp)
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79242	0.22079569315489606	No Hit
CGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	76804	0.21400257965559474	Illumina Single End PCR Primer 1 (100% over 41bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	59398	0.16550342724835965	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52463	0.14618011218779575	No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50915	0.14186684734082347	No Hit
CGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTT	48898	0.13624678584447777	No Hit
CCGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	46017	0.12821932071261266	Illumina Single End PCR Primer 1 (97% over 42bp)
CTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43373	0.12085221977243515	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	42365	0.11804358219766249	Illumina Single End PCR Primer 1 (98% over 50bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	36049	0.10044501580652744	Illumina Single End PCR Primer 1 (100% over 38bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1906683741028345	0.0	0.0	2.2290774402957628E-5	0.0
2	4.197645386490961	2.7863468003697035E-6	0.0	3.900885520517585E-5	0.0
3	4.198085629285419	2.7863468003697035E-6	0.0	5.0154242406654667E-5	0.0
4	5.613937106078479	2.7863468003697035E-6	0.0	5.294058920702437E-5	0.0
5	5.633672800465498	2.7863468003697035E-6	0.0	5.572693600739407E-5	0.0
6	5.6400145257831396	2.7863468003697035E-6	0.0	6.408597640850318E-5	0.0
7	5.730637669118364	2.7863468003697035E-6	0.0	8.080405721072141E-5	0.0
8	6.728893778246417	5.572693600739407E-6	0.0	8.080405721072141E-5	0.0
9	6.734742320180392	5.572693600739407E-6	0.0	1.0588117841404874E-4	0.0
10-11	7.141594927756575	6.965867000924259E-6	0.0	1.12847045414973E-4	0.0
12-13	7.376737520586575	9.752213801293963E-6	0.0	1.1981291241589726E-4	0.0
14-15	7.456453509371752	1.1145387201478814E-5	0.0	1.239924326164518E-4	0.0
16-17	7.520701093894677	1.1145387201478814E-5	0.0	1.3374464641774576E-4	0.0
18-19	7.546905292378753	1.1145387201478814E-5	0.0	1.6578763462199737E-4	0.0
20-21	7.559576204453435	1.1145387201478814E-5	0.0	1.9504427602587925E-4	0.0
22-23	7.565274283660191	1.1145387201478814E-5	0.0	1.992237962264338E-4	0.0
24-25	7.570721591654913	1.1145387201478814E-5	0.0	2.507712120332733E-4	0.0
26-27	7.5776609883612345	1.1145387201478814E-5	0.0	3.650114308484312E-4	0.0
28-29	7.5839344481822675	1.1145387201478814E-5	0.0	4.2073836685582527E-4	0.0
30-31	7.590695518693364	1.9504427602587926E-5	0.0	4.653199156617405E-4	0.0
32-33	7.597286622049639	1.9504427602587926E-5	0.0	4.736789560628496E-4	0.0
34-35	7.603876332232513	2.0897601002772777E-5	0.0	4.931833836654375E-4	0.0
36-37	7.611044209376464	2.2290774402957628E-5	0.0	5.391581058715378E-4	0.0
38-39	7.619693029844811	2.2290774402957628E-5	0.0	5.475171462726467E-4	0.0
40-41	7.629762887181348	2.5077121203327333E-5	0.0	5.725942674759741E-4	0.0
42-43	7.641473902783302	2.5077121203327333E-5	1.3931734001848517E-6	6.283212034833682E-4	0.0
44-45	7.6715580891868935	2.5077121203327333E-5	2.7863468003697035E-6	6.603641916876198E-4	0.0
46-47	8.029677491224609	2.5077121203327333E-5	2.7863468003697035E-6	7.035525670933502E-4	0.0
48-49	8.078017821864224	2.5077121203327333E-5	2.7863468003697035E-6	7.230569946959382E-4	0.0
50-51	8.093615791252693	2.5077121203327333E-5	2.7863468003697035E-6	7.328092084972321E-4	0.0
52-53	8.112012646002135	2.7863468003697035E-5	2.7863468003697035E-6	7.425614222985261E-4	0.0
54-55	8.133432687029977	3.343616160443645E-5	2.7863468003697035E-6	7.537068095000048E-4	0.0
56-57	8.153002593782373	3.343616160443645E-5	2.7863468003697035E-6	7.71818063702408E-4	0.0
58-59	8.189060707725957	3.343616160443645E-5	2.7863468003697035E-6	7.885361445046261E-4	2.7863468003697035E-6
60-61	8.237056924535725	3.343616160443645E-5	2.7863468003697035E-6	8.024678785064747E-4	2.7863468003697035E-6
62-63	8.263893623743487	3.343616160443645E-5	2.7863468003697035E-6	8.177927859085081E-4	2.7863468003697035E-6
64-65	8.305516072247409	3.343616160443645E-5	2.7863468003697035E-6	8.400835603114657E-4	2.7863468003697035E-6
66-67	8.33438959096624	3.343616160443645E-5	2.7863468003697035E-6	8.540152943133141E-4	2.7863468003697035E-6
68-69	8.366913223993556	4.04020286053607E-5	4.179520200554555E-6	8.693402017153475E-4	2.7863468003697035E-6
70-71	8.414307589894443	4.876106900646981E-5	5.572693600739407E-6	8.763060687162718E-4	2.7863468003697035E-6
72-73	8.454694293592404	5.154741580683952E-5	5.572693600739407E-6	8.846651091173809E-4	2.7863468003697035E-6
74-75	8.504423618112	5.4333762607209216E-5	5.572693600739407E-6	9.069558835203386E-4	2.7863468003697035E-6
76-77	8.56825046426807	5.851328280776377E-5	5.572693600739407E-6	9.571101259269932E-4	2.7863468003697035E-6
78-79	8.626607711655012	6.129962960813348E-5	5.572693600739407E-6	9.891531141312448E-4	2.7863468003697035E-6
80-81	8.691309470706397	6.687232320887288E-5	5.572693600739407E-6	0.0010170165821349417	2.7863468003697035E-6
82-83	8.76579270020048	6.965867000924259E-5	5.572693600739407E-6	0.0010309483161367904	2.7863468003697035E-6
84-85	8.851603822611466	6.965867000924259E-5	5.572693600739407E-6	0.0010365210097375297	2.7863468003697035E-6
86-87	8.952220198746216	6.965867000924259E-5	5.572693600739407E-6	0.0010420937033382691	2.7863468003697035E-6
88-89	9.069974000876641	7.383819020979714E-5	5.572693600739407E-6	0.0010462732235388236	2.7863468003697035E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGT	1820	0.0	54.06205	1
CGCGTGT	152925	0.0	53.71356	1
ATACGGC	6685	0.0	52.19011	1
ATCCGGA	3710	0.0	51.899	6
GATCCGG	3790	0.0	51.551685	5
TACGGCG	6505	0.0	51.295982	2
CTCGCGT	580	0.0	48.35235	1
CGGCGAC	13135	0.0	48.02135	1
ACCACCG	74240	0.0	47.692883	1
GGCGACC	13540	0.0	46.33923	2
TGATACG	3785	0.0	45.711872	2
CCACCGA	77315	0.0	45.70995	2
CACCGAG	78920	0.0	44.837498	3
ACGGCGA	7260	0.0	44.589493	3
ACCGAGA	80430	0.0	44.179855	4
CGCTCAT	1455	0.0	43.775925	1
GCGTGTT	188350	0.0	43.507607	2
TCTCGGA	59700	0.0	43.145306	40-41
CCGAGAT	147765	0.0	43.082394	1
CGGAGAT	57875	0.0	42.97795	42-43
>>END_MODULE