FastQCFastQC Report
Fri 27 May 2016
SRR1233055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35889287
Sequences flagged as poor quality0
Sequence length101
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG13257113.69389060306492TruSeq Adapter, Index 2 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7777812.1671676007383485No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5356001.4923673462780132TruSeq Adapter, Index 2 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5161661.4382174825596286No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC3167570.8825948534447061TruSeq Adapter, Index 2 (100% over 42bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2334640.6505116694015125No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT912360.2542151366785303No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT889570.24786505232048772TruSeq Adapter, Index 2 (100% over 46bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880840.24543257156376497No Hit
GCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817970.22791480922984061No Hit
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720570.20077579139423973No Hit
CGGTGGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT576930.1607527059537293TruSeq Adapter, Index 2 (100% over 39bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498610.1389300378132338No Hit
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT487750.13590406518803227TruSeq Adapter, Index 2 (100% over 37bp)
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT465380.12967100739560528No Hit
CGGTGGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA464500.12942580887717273TruSeq Adapter, Index 2 (100% over 40bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT458340.127709419248145No Hit
CTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392360.10932510305930569No Hit
CGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTT377510.10518737806075669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT613250.092.5527341
GTGGTCG241900.089.666413
CGGAGAT375100.088.460231
GTCGCCG99500.086.920976
GGTCGCC102750.085.788845
GGTGGTC264550.085.059452
GGTAGAT411250.083.4616245
GTGGTAG406750.083.392493
GGTCGAG89950.082.209525
TCGCCGA74050.081.775427
GGTGGTA418100.081.1401442
CGCCGTA37200.080.943888
CGCCGAG85100.080.254068
GTAGATC428750.080.1441966
TGGTAGA437400.077.7335054
TCGCCGT35100.077.397447
CGGCACT77900.076.5145348
TCGCAGA113050.076.040637
TGGTCGA84800.075.888874
CGGTCGA13350.075.776234
Produced by FastQC (version 0.11.5)