##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233055_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35889287
Sequences flagged as poor quality	0
Sequence length	101
%GC	27
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89578004154833	34.0	31.0	34.0	28.0	34.0
2	32.03059453368355	34.0	33.0	34.0	30.0	34.0
3	32.50800440811209	34.0	33.0	34.0	30.0	34.0
4	35.62044863137014	37.0	37.0	37.0	35.0	37.0
5	35.58334285102961	37.0	37.0	37.0	35.0	37.0
6	35.57759489621513	37.0	37.0	37.0	35.0	37.0
7	35.41936283103089	37.0	37.0	37.0	33.0	37.0
8	35.10151884599992	37.0	35.0	37.0	32.0	37.0
9	36.459262954987096	39.0	38.0	39.0	32.0	39.0
10-11	36.549720965479196	39.0	38.0	39.0	32.5	39.0
12-13	36.8139727462404	39.0	39.0	39.0	34.0	39.0
14-15	38.11538624325415	41.0	40.0	41.0	33.5	41.0
16-17	38.05314871538128	41.0	39.5	41.0	33.5	41.0
18-19	37.68643043814161	41.0	38.0	41.0	34.0	41.0
20-21	37.29212444370934	41.0	37.5	41.0	33.0	41.0
22-23	36.668920589032595	40.0	35.5	41.0	32.0	41.0
24-25	36.417982502689455	40.0	35.0	41.0	31.5	41.0
26-27	35.917989538215124	40.0	35.0	41.0	28.0	41.0
28-29	35.32199686775611	40.0	35.0	41.0	20.5	41.0
30-31	34.81652236501661	40.0	35.0	41.0	12.0	41.0
32-33	34.37731957450144	40.0	35.0	41.0	6.0	41.0
34-35	34.04927201535099	40.0	35.0	41.0	2.0	41.0
36-37	33.76292564686504	40.0	35.0	41.0	2.0	41.0
38-39	33.57176566366448	39.5	35.0	41.0	2.0	41.0
40-41	33.21823675404864	39.0	35.0	41.0	2.0	41.0
42-43	32.87446160465656	38.0	35.0	40.5	2.0	41.0
44-45	32.781066756773406	38.0	35.0	40.0	2.0	41.0
46-47	32.65064090016611	38.0	35.0	40.0	2.0	41.0
48-49	32.29978668843435	38.0	34.5	40.0	2.0	41.0
50-51	32.10185511626352	37.0	34.0	40.0	2.0	41.0
52-53	31.944558734198313	37.0	34.0	40.0	2.0	41.0
54-55	31.70478470358021	36.5	33.0	40.0	2.0	41.0
56-57	31.495055920169158	36.0	33.0	40.0	2.0	41.0
58-59	31.098824824800783	35.5	33.0	39.5	2.0	41.0
60-61	30.765588043027993	35.0	33.0	39.0	2.0	40.0
62-63	30.42835455326822	35.0	32.0	39.0	2.0	40.0
64-65	29.86716194724069	35.0	31.0	38.0	2.0	40.0
66-67	29.52789641655461	35.0	31.0	38.0	2.0	40.0
68-69	29.0212642424465	35.0	29.5	37.5	2.0	40.0
70-71	28.1767410703924	35.0	27.5	36.5	2.0	39.0
72-73	27.16416573001297	35.0	22.5	36.0	2.0	39.0
74-75	26.15679812753037	34.0	6.0	35.5	2.0	38.5
76-77	24.999593458070095	32.5	2.0	35.0	2.0	37.0
78-79	25.08091761477457	33.0	2.0	35.0	2.0	37.0
80-81	24.609298437720426	33.0	2.0	35.0	2.0	36.5
82-83	24.152148662078464	32.5	2.0	35.0	2.0	36.0
84-85	23.709819980541823	32.0	2.0	35.0	2.0	36.0
86-87	23.164095918093885	31.5	2.0	35.0	2.0	35.0
88-89	22.647617254140492	31.0	2.0	34.0	2.0	35.0
90-91	22.08485752308203	30.5	2.0	34.0	2.0	35.0
92-93	21.444995884147822	29.0	2.0	34.0	2.0	35.0
94-95	20.801331397305276	29.0	2.0	34.0	2.0	35.0
96-97	19.93306491432945	28.0	2.0	33.0	2.0	35.0
98-99	18.705114732426978	26.5	2.0	33.0	2.0	35.0
100-101	12.783447704046058	2.0	2.0	26.5	2.0	30.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.13728355749211474
1101	2	0.1279706534598688
1101	3	0.1528681043274034
1101	4	0.15576281190882924
1101	5	0.1693557220964621
1101	6	0.15294878874549056
1101	7	0.11015601815945786
1101	8	-0.028805304260309583
1101	9	0.0448156231617034
1101	10-11	0.13310324242710436
1101	12-13	0.14400075919176203
1101	14-15	0.365878859715167
1101	16-17	0.22995414960050198
1101	18-19	0.1638847074349954
1101	20-21	0.13572067119334008
1101	22-23	0.38600734640731815
1101	24-25	0.20900139584554722
1101	26-27	0.13419989105559438
1101	28-29	0.11352555295129463
1101	30-31	0.10258278823723543
1101	32-33	0.0407378123314146
1101	34-35	0.031769659303243714
1101	36-37	0.07249429455033862
1101	38-39	0.08504060644157363
1101	40-41	0.04324711344067822
1101	42-43	0.0806035546317716
1101	44-45	0.05328813186549297
1101	46-47	-0.02153507303977875
1101	48-49	0.13427690510319223
1101	50-51	0.12124028894664463
1101	52-53	-0.026965808422307447
1101	54-55	-0.10307350864191278
1101	56-57	-0.07843218252985551
1101	58-59	-0.10941333759874183
1101	60-61	-0.17478805141252707
1101	62-63	-0.19921368699622732
1101	64-65	-0.4351352039306917
1101	66-67	-0.2813152715221534
1101	68-69	-0.2760972771347774
1101	70-71	-0.4364358537043884
1101	72-73	-0.4499126233017705
1101	74-75	-0.2317070088841362
1101	76-77	0.07972697149818231
1101	78-79	-0.13533662931006418
1101	80-81	-0.26363104327564457
1101	82-83	-0.26806257632595276
1101	84-85	-0.2987276809699182
1101	86-87	-0.2702460478812192
1101	88-89	-0.3002243962160662
1101	90-91	-0.42062222570232066
1101	92-93	-0.5056661891060514
1101	94-95	-0.7321202605961687
1101	96-97	-0.8669658077615168
1101	98-99	-0.6519075149550098
1101	100-101	0.0011163580507176363
1102	1	0.011435987472747655
1102	2	0.06253427095504804
1102	3	0.07324036398971145
1102	4	0.08808590965806218
1102	5	0.07876341137664866
1102	6	0.09819944023283256
1102	7	-0.07105671171490968
1102	8	-0.20636733569948262
1102	9	-0.07918170607465669
1102	10-11	0.03456906582547248
1102	12-13	0.06558503194290921
1102	14-15	0.312242807777352
1102	16-17	0.21154305716866162
1102	18-19	0.0989614158166674
1102	20-21	0.06769314608059318
1102	22-23	0.3059228955529747
1102	24-25	0.09897906806549628
1102	26-27	0.04110048371900632
1102	28-29	0.0599145320083565
1102	30-31	0.06460516821748996
1102	32-33	-0.04844827277421615
1102	34-35	-0.009676486610295854
1102	36-37	0.01278408434667
1102	38-39	-0.03805772430880694
1102	40-41	-0.025957177719327262
1102	42-43	-0.00726169200785165
1102	44-45	-0.028833288522953637
1102	46-47	-0.025413860818154888
1102	48-49	0.10229667403194753
1102	50-51	0.06803332732093281
1102	52-53	-0.024678660193909963
1102	54-55	-0.11112459956841647
1102	56-57	-0.054970690166680214
1102	58-59	-0.12951250163136407
1102	60-61	-0.19241431174689438
1102	62-63	-0.17154805705892429
1102	64-65	-0.24332432475726762
1102	66-67	-0.2580089877680649
1102	68-69	-0.32241197516810516
1102	70-71	-0.4213241565026635
1102	72-73	-0.39393385027576144
1102	74-75	-0.14298013221712935
1102	76-77	0.007098488471964259
1102	78-79	-0.15861781576079892
1102	80-81	-0.2862429696662545
1102	82-83	-0.20003202055260516
1102	84-85	-0.2870846536331264
1102	86-87	-0.32205851095517346
1102	88-89	-0.3717198050295707
1102	90-91	-0.43075620705220885
1102	92-93	-0.5196309736073133
1102	94-95	-0.6673310315419911
1102	96-97	-0.8206036278647595
1102	98-99	-0.6633486316251052
1102	100-101	-0.1132218970327532
1103	1	0.08209291270425823
1103	2	0.10026364223424622
1103	3	0.061808902197640236
1103	4	0.08380677242895018
1103	5	0.058226350529800186
1103	6	0.06380906727292057
1103	7	0.06727867511328611
1103	8	0.026024197236495183
1103	9	0.08085956260244842
1103	10-11	0.16639560231833883
1103	12-13	0.10294410749724392
1103	14-15	0.2988820302567561
1103	16-17	0.25197341639117354
1103	18-19	0.12040437586221486
1103	20-21	0.14976874465895662
1103	22-23	0.3703621152214893
1103	24-25	0.20269445089315496
1103	26-27	0.14800489256106886
1103	28-29	0.17180404080011868
1103	30-31	0.13481155885096996
1103	32-33	0.11170656034705218
1103	34-35	0.06199917754857864
1103	36-37	0.059646405879050235
1103	38-39	0.07706148999471196
1103	40-41	0.08213211984573121
1103	42-43	0.07945999493443168
1103	44-45	0.06919824111307094
1103	46-47	0.05438263188354853
1103	48-49	0.2510087033484609
1103	50-51	0.2152098409343708
1103	52-53	0.09961189186100583
1103	54-55	0.009032880955913924
1103	56-57	0.0016574966934328472
1103	58-59	-0.04621348251445667
1103	60-61	-0.08642823334053062
1103	62-63	-0.09436735775001637
1103	64-65	-0.11268325543003499
1103	66-67	-0.09026667421206014
1103	68-69	-0.177192919293649
1103	70-71	-0.2664482479472525
1103	72-73	-0.2095671836998001
1103	74-75	-0.0725040814514415
1103	76-77	0.15718830976840437
1103	78-79	-0.1453457081398426
1103	80-81	-0.2340022568545166
1103	82-83	-0.2551262652509365
1103	84-85	-0.19827680318150698
1103	86-87	-0.1919974995338336
1103	88-89	-0.24015144963949808
1103	90-91	-0.2250560800018242
1103	92-93	-0.3884143899584025
1103	94-95	-0.4908579107863389
1103	96-97	-0.6197337847679449
1103	98-99	-0.4970103996611108
1103	100-101	-0.007043779448309806
1104	1	0.09657070598830231
1104	2	0.09614580450167409
1104	3	0.0693429531493095
1104	4	0.09907436117251933
1104	5	0.07732272914466165
1104	6	0.06834201144350516
1104	7	-0.0024538553275590402
1104	8	-0.010247688158884216
1104	9	0.023759449705536895
1104	10-11	0.10535982750905504
1104	12-13	0.06443492851941102
1104	14-15	0.24656989309998067
1104	16-17	0.16716331374563254
1104	18-19	0.06423023836051556
1104	20-21	0.08290127466491981
1104	22-23	0.3350514778758722
1104	24-25	0.17071586807244188
1104	26-27	0.09160176103024753
1104	28-29	0.05009874581111973
1104	30-31	0.030377282797502403
1104	32-33	0.04421815998119172
1104	34-35	0.05834806602659626
1104	36-37	0.06503184312040844
1104	38-39	0.05525532224382346
1104	40-41	0.0702553159633581
1104	42-43	0.06358733206820233
1104	44-45	0.04725811590694917
1104	46-47	0.039186275067251586
1104	48-49	0.22363431158099445
1104	50-51	0.14367344468822552
1104	52-53	0.062410301530658074
1104	54-55	0.014531452776598286
1104	56-57	-0.025156418120008794
1104	58-59	-0.05133134571726217
1104	60-61	-0.11501950006226025
1104	62-63	-0.1178079728693966
1104	64-65	-0.11590530946963185
1104	66-67	-0.0596367766512067
1104	68-69	-0.15055135184536894
1104	70-71	-0.1440341542929744
1104	72-73	-0.04603900191596111
1104	74-75	-0.01197724580125481
1104	76-77	0.20337750122842735
1104	78-79	0.04225959876520591
1104	80-81	-0.1796048320570307
1104	82-83	-0.1696751052814065
1104	84-85	-0.13023079518999836
1104	86-87	-0.010071233251359502
1104	88-89	-0.1030297817974386
1104	90-91	-0.18807375528044545
1104	92-93	-0.3680099584145573
1104	94-95	-0.41761519316004936
1104	96-97	-0.49367795229532163
1104	98-99	-0.4407493740527251
1104	100-101	-0.058106639210890876
1105	1	0.1841755023121756
1105	2	0.09364756566102983
1105	3	0.056095590025663
1105	4	0.09735428020337622
1105	5	0.04753968103332795
1105	6	0.04935789138588831
1105	7	0.11508426696861562
1105	8	0.19252738001858205
1105	9	0.1398303946560162
1105	10-11	0.1603229440909999
1105	12-13	0.0677516952700401
1105	14-15	0.2645604289789816
1105	16-17	0.14495189295482902
1105	18-19	0.04802132972918827
1105	20-21	0.043107582276931566
1105	22-23	0.2844021104320902
1105	24-25	0.11659674061036895
1105	26-27	0.020728360913771837
1105	28-29	0.022618334282434205
1105	30-31	0.004031315194602314
1105	32-33	0.037007497086158025
1105	34-35	0.04083434678062048
1105	36-37	0.027671672012616
1105	38-39	-0.005503034679186669
1105	40-41	0.04061154517379606
1105	42-43	0.042755016507072696
1105	44-45	0.05267992259704357
1105	46-47	0.04255109411636937
1105	48-49	0.24957245221889934
1105	50-51	0.14615886720614668
1105	52-53	0.07166961419892104
1105	54-55	0.0027661594737793394
1105	56-57	-0.05475761795478107
1105	58-59	-0.07358663379485719
1105	60-61	-0.06232451135609196
1105	62-63	-0.05815410918250663
1105	64-65	-0.06857693641737228
1105	66-67	0.010917726896479962
1105	68-69	-0.08513548297687734
1105	70-71	-0.08080336518274933
1105	72-73	-0.027074073819285616
1105	74-75	0.0014581133914361999
1105	76-77	0.09670311550097566
1105	78-79	0.030283874489324347
1105	80-81	-0.0901221658948188
1105	82-83	-0.09921971279195674
1105	84-85	-0.12581766525364557
1105	86-87	-0.06417650401767716
1105	88-89	-0.16869413628703356
1105	90-91	-0.2401597455890716
1105	92-93	-0.3021429735447043
1105	94-95	-0.3579765082254305
1105	96-97	-0.3658847950772781
1105	98-99	-0.2388007290075187
1105	100-101	0.07925828508972899
1106	1	0.18114266168324988
1106	2	0.09982867057527045
1106	3	0.04187334242444507
1106	4	0.06691971420065101
1106	5	0.027193889883044164
1106	6	0.029233299239457722
1106	7	0.07780100521856781
1106	8	0.2064777804162361
1106	9	0.18675498528450163
1106	10-11	0.15979192638096862
1106	12-13	0.06349510137896175
1106	14-15	0.24484995878861326
1106	16-17	0.15439204826659392
1106	18-19	0.024547435584260313
1106	20-21	-0.0130881067957489
1106	22-23	0.23272996645689403
1106	24-25	0.061604764225997144
1106	26-27	7.947768932083932E-4
1106	28-29	0.014139492130453846
1106	30-31	0.014269069967575376
1106	32-33	0.005814372858104377
1106	34-35	0.029379561836556434
1106	36-37	-8.332807797444275E-4
1106	38-39	-0.021372435599246842
1106	40-41	-0.007636858503097699
1106	42-43	-0.06469327068680997
1106	44-45	-0.07008783638337235
1106	46-47	-0.05851207824078131
1106	48-49	0.1445471661851201
1106	50-51	0.09868438803452051
1106	52-53	-0.02205951805720474
1106	54-55	-0.09606227356026764
1106	56-57	-0.11453514102744222
1106	58-59	-0.12411565957944148
1106	60-61	-0.1745389382206497
1106	62-63	-0.13305237357867483
1106	64-65	-0.07696925932466314
1106	66-67	-0.03315559242359001
1106	68-69	-0.1445835339218391
1106	70-71	-0.0961893996072476
1106	72-73	0.0767756410798448
1106	74-75	1.5675706066531347E-4
1106	76-77	0.11068826065490711
1106	78-79	-0.03818146111760967
1106	80-81	-0.16953023302465198
1106	82-83	-0.2180327448657735
1106	84-85	-0.1651797165347766
1106	86-87	-9.10240323914735E-4
1106	88-89	-0.11955990828020191
1106	90-91	-0.14870422249364523
1106	92-93	-0.27671908854226146
1106	94-95	-0.2966566643400945
1106	96-97	-0.2636437343195297
1106	98-99	-0.2618308558409659
1106	100-101	-0.01620694634137365
1107	1	0.16602661724204992
1107	2	0.06339133099261574
1107	3	0.01166010792432104
1107	4	0.06374287820430169
1107	5	-0.0059160843206313984
1107	6	-0.0533939261684111
1107	7	0.10523863862717775
1107	8	0.21200455416263253
1107	9	0.12027882340922247
1107	10-11	0.09642693529058732
1107	12-13	-0.0277478698438145
1107	14-15	0.1952505531905686
1107	16-17	0.1067696593412748
1107	18-19	-0.059480292769855225
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2307	94-95	-0.04357790595690503
2307	96-97	-0.14902936367294473
2307	98-99	-0.33493671319379814
2307	100-101	0.07871633066010197
2308	1	0.1818100875875821
2308	2	0.027575191449038527
2308	3	-0.03316436017286151
2308	4	0.03180786636288957
2308	5	-0.06637314771936076
2308	6	-0.3098441948717934
2308	7	0.005444613818944788
2308	8	0.20426870830990396
2308	9	0.09923528905673606
2308	10-11	-0.17023031100473673
2308	12-13	-0.27167349467237756
2308	14-15	0.025379585649595526
2308	16-17	-0.04437693338017823
2308	18-19	-0.3423888448126249
2308	20-21	-0.2679763013701191
2308	22-23	0.015355649964959639
2308	24-25	-0.09524521907025729
2308	26-27	-0.10802903127768815
2308	28-29	-0.1225112091624041
2308	30-31	-0.2051263031823396
2308	32-33	-0.24279943188262365
2308	34-35	-0.37153148423332283
2308	36-37	-0.33322096057540307
2308	38-39	-0.2424319926589007
2308	40-41	-0.24378899224560513
2308	42-43	-0.5784716657224536
2308	44-45	-0.3079094508818869
2308	46-47	-0.2733059102678155
2308	48-49	-0.12692177247558334
2308	50-51	-0.13172410897212927
2308	52-53	-0.21110047769983353
2308	54-55	-0.1321186887590322
2308	56-57	-0.11052016744768878
2308	58-59	-0.07691095639079748
2308	60-61	-0.022028845105577233
2308	62-63	-0.07881120783418183
2308	64-65	-0.0664874098595476
2308	66-67	-0.15869825211514765
2308	68-69	-0.20522759897033538
2308	70-71	0.09452447324255076
2308	72-73	0.18019155110321705
2308	74-75	0.08125439359218944
2308	76-77	0.14401356596233583
2308	78-79	-0.05160444250591212
2308	80-81	-0.12514297532650076
2308	82-83	-0.15270701870233339
2308	84-85	-0.10022632321270564
2308	86-87	-0.22196999906531545
2308	88-89	-0.2636574915638832
2308	90-91	-0.23605385941915458
2308	92-93	-0.05970729334861247
2308	94-95	-0.43116500859649065
2308	96-97	-0.5025339961651092
2308	98-99	-0.4138889125836762
2308	100-101	-0.1574985616262925
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	408145.0
3	618532.0
4	173006.0
5	115446.0
6	211771.0
7	211888.0
8	158873.0
9	259878.0
10	474649.0
11	642681.0
12	554410.0
13	401421.0
14	311398.0
15	272031.0
16	256848.0
17	247162.0
18	240714.0
19	240750.0
20	253280.0
21	277313.0
22	311721.0
23	361687.0
24	425702.0
25	522787.0
26	710856.0
27	1054320.0
28	956077.0
29	645204.0
30	682444.0
31	699559.0
32	888289.0
33	1335705.0
34	2645102.0
35	4396852.0
36	6040508.0
37	6087946.0
38	1764246.0
39	30086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.69313688015666	20.995986338139375	10.18790562036144	40.12297116134253
2	21.911477827410355	19.453513110301508	45.90813316641422	12.726875895873924
3	19.64345237619237	25.511206171356932	36.81500833382396	18.030333118626736
4	17.378319608299826	28.291010629439366	38.51581671154403	15.81485305071678
5	21.36167263506795	28.826429457904805	34.56197667008542	15.249921236941821
6	21.702417771631964	32.483456692800836	35.14506710595839	10.669058429608814
7	13.723989557106552	20.793979551613827	54.493782503954456	10.988248387325164
8	10.329461267926554	28.790998271991302	45.393139183846145	15.486401276235998
9	15.0996624286087	25.953271867333395	46.25216232683657	12.694903377221339
10-11	16.076011918892682	34.654622109579364	37.73012886313558	11.539237108392376
12-13	14.912087599617449	27.597903558841736	42.38457009232036	15.105438749220463
14-15	12.59677583951991	30.924416998852372	42.32185250780615	14.156954653821572
16-17	14.334539293368886	30.401322970006976	40.884640690029485	14.37949704659465
18-19	15.136300980289743	29.13587834720707	43.2855701479943	12.442250524508887
20-21	13.884074933001594	28.97244378245798	42.20390335422378	14.939577930316645
22-23	14.44256093587373	33.4239040029941	40.05898584886866	12.074549212263515
24-25	13.073884694337895	31.66916913116719	39.55578721862042	15.701158955874492
26-27	12.164277601837005	30.538968355654433	40.63001725278075	16.66673678972781
28-29	13.636502447095145	32.90957270898137	38.78947776254234	14.664447081381137
30-31	14.312231948213405	31.63468251681902	40.321118666971564	13.731966867996013
32-33	12.019650128474472	35.11885779887566	37.22890997580862	15.632582096841249
34-35	12.463302767759082	31.912881133581728	37.30695039998984	18.316865698669353
36-37	14.13490505789479	36.49327904408118	36.89192638239205	12.479889515631983
38-39	15.177931787834067	32.480476694897845	39.65789568346677	12.683695833801323
40-41	11.391883432090042	36.54901425912545	39.98249748234998	12.076604826434528
42-43	12.29313764398004	32.65165545472472	40.137788563671535	14.917418337623706
44-45	12.355774858386013	33.27779958403743	39.875675156210264	14.49075040136629
46-47	13.801951137430224	33.79150592469146	40.82779181690989	11.578751120968434
48-49	13.052786068016644	35.00304322304592	40.29265346995346	11.651517238983978
50-51	13.043300599984613	33.90309984759434	38.00485646235581	15.048743090065233
52-53	15.53039914404003	32.90183974300916	38.09530934655396	13.472451766396846
54-55	11.218705765979479	34.1021005045847	39.16315128085346	15.51604244858236
56-57	12.844510586199478	33.760894304690915	41.88814264238287	11.50645246672674
58-59	11.962552787879936	34.4581015533485	38.921787002785265	14.657558655986305
60-61	13.64239362774823	34.219005925659154	36.86158450168282	15.277015944909794
62-63	12.912767288040913	34.79287196968042	41.034731507120476	11.259629235158194
64-65	13.01860519244436	38.62251070698829	35.74614155464482	12.612742545922535
66-67	12.33613685112935	41.07475252212873	35.78670480267135	10.802405824070565
68-69	11.225039474144348	42.67998544893802	34.92354771164209	11.171427365275544
70-71	11.367424813984774	43.12814388191962	34.263838521698354	11.240592782397254
72-73	11.165374027301425	43.766004936184544	34.14616689847781	10.922454138036217
74-75	11.803367689591573	42.94775606097557	34.0546815981655	11.194194651267354
76-77	11.556247383042205	42.62731709521732	34.09142527264218	11.725010249098299
78-79	11.6230118982715	42.725574561103464	34.03138328117091	11.62003025945413
80-81	11.87116679689153	42.16806234641707	34.09669900071268	11.864071855978718
82-83	12.048883228689974	41.76134369324747	34.26009011599237	11.929682962070192
84-85	12.488055464200198	41.07990573771514	34.26865843686704	12.163380361217618
86-87	12.402350937487014	41.47832954888075	33.93795350065743	12.181366012974811
88-89	12.543672165163228	40.89687052874822	34.01066254741029	12.548794758678255
90-91	12.563387115495905	41.063855106564986	33.34943235616538	13.023325421773729
92-93	12.76011906666933	41.129624675874595	33.43089658265384	12.679359674802235
94-95	13.038115942600445	41.10634717116057	32.95034304700849	12.905193839230494
96-97	12.549329562700745	41.55446661859744	32.61630536740113	13.279898451300687
98-99	13.26021205842254	41.10492738161302	32.328143618159906	13.306716941804536
100-101	13.663126244812895	40.5599346126445	31.037781845562705	14.739157296979903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	714694.0
1	728379.0
2	606452.0
3	516521.5
4	633194.5
5	771231.0
6	767719.0
7	675251.0
8	641035.0
9	606287.0
10	567926.0
11	532759.0
12	516339.0
13	496402.5
14	527095.5
15	527581.0
16	451773.0
17	423259.0
18	417608.5
19	422285.5
20	440972.5
21	468859.0
22	504263.5
23	548901.0
24	605541.5
25	686314.5
26	770015.0
27	841074.0
28	888591.5
29	917647.5
30	937047.5
31	927375.0
32	914097.5
33	909965.5
34	909499.0
35	902729.5
36	912968.0
37	923279.0
38	913378.0
39	895730.5
40	886818.0
41	886421.5
42	852518.5
43	831297.0
44	828074.0
45	798456.0
46	732547.5
47	640289.0
48	535176.5
49	427435.5
50	335010.5
51	259254.0
52	200191.0
53	157672.5
54	122666.5
55	94700.5
56	73903.5
57	57674.0
58	44746.0
59	33573.5
60	23954.0
61	16922.0
62	12395.0
63	9218.0
64	6594.5
65	4627.5
66	3323.0
67	2387.5
68	1671.5
69	1183.0
70	865.0
71	699.5
72	581.0
73	442.5
74	314.5
75	215.5
76	162.5
77	122.5
78	84.0
79	66.0
80	54.5
81	39.0
82	27.5
83	23.0
84	25.5
85	24.5
86	18.5
87	18.0
88	15.5
89	13.5
90	14.0
91	17.5
92	11.5
93	6.0
94	6.0
95	6.5
96	7.5
97	6.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.264817186253937
2	0.0012482833665656272
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	7.968951849057353E-4
10-11	1.3095829961737605E-4
12-13	5.029355974667315E-4
14-15	2.772415066367855E-4
16-17	1.0588117841404872E-4
18-19	0.0
20-21	0.0
22-23	2.786346800369704E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.560354208207034E-4
34-35	0.0
36-37	1.3095829961737605E-4
38-39	0.0
40-41	4.5835404866081627E-4
42-43	6.687232320887288E-5
44-45	0.0
46-47	3.301820958438099E-4
48-49	0.0014697979371950188
50-51	5.572693600739407E-4
52-53	6.283212034833682E-4
54-55	7.453477690988957E-4
56-57	0.0038563039717116696
58-59	0.0010225892757356812
60-61	0.002436660276923306
62-63	0.0018473479286451135
64-65	0.003233555461829041
66-67	0.006382127346246807
68-69	0.005216041210292085
70-71	0.01672226032241878
72-73	0.025482534662781123
74-75	0.012971837529121153
76-77	0.0021287689554824536
78-79	0.008308886158702455
80-81	0.012712707276686771
82-83	0.01665678117261009
84-85	0.02323952548848351
86-87	0.0169131250782441
88-89	0.025145386699936392
90-91	0.020039406188258908
92-93	0.010246790358359585
94-95	0.010051746082333706
96-97	0.011809930913366988
98-99	0.013607124599605449
100-101	0.004498556909196887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5889287E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.40705110208576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6733698243852	32.193659259693106
2	11.411719088272513	9.222278327249498
3	3.322860498440319	4.028009818967403
4	1.4820310596379023	2.395380190466681
5	0.8790337489093321	1.7759580806318702
6	0.5475911884897201	1.32759270818136
7	0.3991323162346989	1.1289431929012534
8	0.30526571746729064	0.9867909956332551
9	0.23000992530964473	0.8364620525376368
>10	1.4912789371214292	11.435924243881662
>50	0.13943342969559208	3.917639668624283
>100	0.10131759603220858	7.945169611550098
>500	0.009819415495398859	2.703972671174306
>1k	0.006307835089078004	4.820784355355908
>5k	4.976516519757417E-4	1.3182551295377622
>10k+	3.3176776762028847E-4	13.963179693614117
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG	1325711	3.69389060306492	TruSeq Adapter, Index 2 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	777781	2.1671676007383485	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	535600	1.4923673462780132	TruSeq Adapter, Index 2 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	516166	1.4382174825596286	No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC	316757	0.8825948534447061	TruSeq Adapter, Index 2 (100% over 42bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233464	0.6505116694015125	No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT	91236	0.2542151366785303	No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT	88957	0.24786505232048772	TruSeq Adapter, Index 2 (100% over 46bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88084	0.24543257156376497	No Hit
GCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81797	0.22791480922984061	No Hit
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72057	0.20077579139423973	No Hit
CGGTGGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT	57693	0.1607527059537293	TruSeq Adapter, Index 2 (100% over 39bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49861	0.1389300378132338	No Hit
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT	48775	0.13590406518803227	TruSeq Adapter, Index 2 (100% over 37bp)
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46538	0.12967100739560528	No Hit
CGGTGGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA	46450	0.12942580887717273	TruSeq Adapter, Index 2 (100% over 40bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	45834	0.127709419248145	No Hit
CTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39236	0.10932510305930569	No Hit
CGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTT	37751	0.10518737806075669	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.179509055167354	0.0	0.0	2.5077121203327333E-5	0.0
2	4.186661607403903	2.7863468003697035E-6	0.0	3.622250840480615E-5	0.0
3	4.186987609979546	2.7863468003697035E-6	0.0	4.4581548805915255E-5	0.0
4	4.468790366328537	2.7863468003697035E-6	0.0	5.294058920702437E-5	0.0
5	4.488294793931125	2.7863468003697035E-6	0.0	5.851328280776377E-5	2.7863468003697035E-6
6	4.493873060225465	2.7863468003697035E-6	0.0	7.523136360998199E-5	2.7863468003697035E-6
7	4.5712136883633265	2.7863468003697035E-6	0.0	1.1145387201478814E-4	2.7863468003697035E-6
8	5.580353268093623	2.7863468003697035E-6	0.0	1.1702656561552755E-4	2.7863468003697035E-6
9	5.586413572384427	2.7863468003697035E-6	0.0	1.4210368681885489E-4	2.7863468003697035E-6
10-11	5.901226179277398	5.572693600739407E-6	0.0	1.5185590062014883E-4	2.7863468003697035E-6
12-13	6.110578346123176	6.965867000924259E-6	0.0	1.7275350162292163E-4	2.7863468003697035E-6
14-15	6.208172929152925	8.359040401109112E-6	0.0	1.9504427602587925E-4	2.7863468003697035E-6
16-17	6.2893601090486975	8.359040401109112E-6	0.0	2.0897601002772777E-4	2.7863468003697035E-6
18-19	6.304697555011332	8.359040401109112E-6	0.0	2.3823265143160967E-4	2.7863468003697035E-6
20-21	6.315171432633923	8.359040401109112E-6	0.0	2.63309772634937E-4	2.7863468003697035E-6
22-23	6.32216098358265	8.359040401109112E-6	0.0	2.688824662356764E-4	2.7863468003697035E-6
24-25	6.328779950406928	8.359040401109112E-6	0.0	3.134640150415917E-4	2.7863468003697035E-6
26-27	6.337055400404026	8.359040401109112E-6	0.0	3.956612456524979E-4	2.7863468003697035E-6
28-29	6.344362594887995	8.359040401109112E-6	0.0	4.4720866145933744E-4	2.7863468003697035E-6
30-31	6.352027834935813	8.359040401109112E-6	1.3931734001848517E-6	5.071151176672861E-4	5.572693600739407E-6
32-33	6.359596946019017	1.1145387201478814E-5	2.7863468003697035E-6	5.182605048687648E-4	5.572693600739407E-6
34-35	6.366998876294199	1.3931734001848518E-5	2.7863468003697035E-6	5.503034930730165E-4	5.572693600739407E-6
36-37	6.37492463976785	1.6718080802218223E-5	2.7863468003697035E-6	5.934918684787468E-4	5.572693600739407E-6
38-39	6.384200388266281	1.9504427602587926E-5	2.7863468003697035E-6	6.116031226811499E-4	5.572693600739407E-6
40-41	6.394838660350093	1.9504427602587926E-5	2.7863468003697035E-6	6.325007236839227E-4	5.572693600739407E-6
42-43	6.406945337197699	1.9504427602587926E-5	2.7863468003697035E-6	6.910140064916865E-4	5.572693600739407E-6
44-45	6.419598138018178	1.9504427602587926E-5	2.7863468003697035E-6	7.355955552976018E-4	5.572693600739407E-6
46-47	6.433722129949252	1.9504427602587926E-5	2.7863468003697035E-6	7.815702775037019E-4	5.572693600739407E-6
48-49	6.447815472065522	1.9504427602587926E-5	2.7863468003697035E-6	8.136132657079534E-4	5.572693600739407E-6
50-51	6.464105848633883	1.9504427602587926E-5	5.572693600739407E-6	8.191859593086929E-4	5.572693600739407E-6
52-53	6.481279497137962	1.9504427602587926E-5	5.572693600739407E-6	8.289381731099868E-4	5.572693600739407E-6
54-55	6.498912892864102	1.9504427602587926E-5	6.965867000924259E-6	8.414767337116505E-4	5.572693600739407E-6
56-57	6.5164529459724285	2.5077121203327333E-5	8.359040401109112E-6	8.498357741127595E-4	5.572693600739407E-6
58-59	6.5369284154349465	2.5077121203327333E-5	8.359040401109112E-6	8.665538549149778E-4	5.572693600739407E-6
60-61	6.558211925469569	2.5077121203327333E-5	8.359040401109112E-6	8.735197219159021E-4	5.572693600739407E-6
62-63	6.582526980822996	3.064981480406674E-5	8.359040401109112E-6	8.846651091173809E-4	5.572693600739407E-6
64-65	6.6090822590039195	3.064981480406674E-5	8.359040401109112E-6	9.055627101201537E-4	5.572693600739407E-6
66-67	6.635689084600649	3.064981480406674E-5	8.359040401109112E-6	9.292466579232961E-4	5.572693600739407E-6
68-69	6.664693561619098	3.204298820425159E-5	8.359040401109112E-6	9.362125249242204E-4	5.572693600739407E-6
70-71	6.698890395900035	3.343616160443645E-5	8.359040401109112E-6	9.445715653253296E-4	5.572693600739407E-6
72-73	6.735450052267686	3.4829335004621294E-5	8.359040401109112E-6	9.473579121256992E-4	5.572693600739407E-6
74-75	6.777200672724426	3.622250840480615E-5	8.359040401109112E-6	9.710418599288416E-4	5.572693600739407E-6
76-77	6.825058965367576	3.622250840480615E-5	8.359040401109112E-6	0.001071350344742151	5.572693600739407E-6
78-79	6.880992091038198	3.622250840480615E-5	8.359040401109112E-6	0.0011145387201478814	6.965867000924259E-6
80-81	6.945721713557585	3.622250840480615E-5	8.359040401109112E-6	0.0011493680551525028	8.359040401109112E-6
82-83	7.020671656140731	3.622250840480615E-5	8.359040401109112E-6	0.0011744451763558302	8.359040401109112E-6
84-85	7.107457999931846	3.622250840480615E-5	8.359040401109112E-6	0.0011814110433567542	8.359040401109112E-6
86-87	7.209025913498922	3.622250840480615E-5	9.752213801293963E-6	0.0011981291241589726	8.359040401109112E-6
88-89	7.329150896756461	3.622250840480615E-5	1.1145387201478814E-5	0.0012023086443595271	9.752213801293963E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	61325	0.0	92.552734	1
GTGGTCG	24190	0.0	89.66641	3
CGGAGAT	37510	0.0	88.46023	1
GTCGCCG	9950	0.0	86.92097	6
GGTCGCC	10275	0.0	85.78884	5
GGTGGTC	26455	0.0	85.05945	2
GGTAGAT	41125	0.0	83.461624	5
GTGGTAG	40675	0.0	83.39249	3
GGTCGAG	8995	0.0	82.20952	5
TCGCCGA	7405	0.0	81.77542	7
GGTGGTA	41810	0.0	81.140144	2
CGCCGTA	3720	0.0	80.94388	8
CGCCGAG	8510	0.0	80.25406	8
GTAGATC	42875	0.0	80.144196	6
TGGTAGA	43740	0.0	77.733505	4
TCGCCGT	3510	0.0	77.39744	7
CGGCACT	7790	0.0	76.514534	8
TCGCAGA	11305	0.0	76.04063	7
TGGTCGA	8480	0.0	75.88887	4
CGGTCGA	1335	0.0	75.77623	4
>>END_MODULE