FastQCFastQC Report
Fri 27 May 2016
SRR1233054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25779893
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2645451.0261679518995677Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1638910.6357318860865715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1236130.47949384429175096No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1221710.4739003377554748Illumina Single End PCR Primer 1 (100% over 50bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT801910.3110602514913464No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC549920.213313530820318Illumina Single End PCR Primer 1 (97% over 44bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT528980.2051909214673622Illumina Single End PCR Primer 1 (100% over 43bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT472610.18332504328082355No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT299870.11631933460701331No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG268490.10414705755372995Illumina Single End PCR Primer 1 (98% over 50bp)
GCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264320.10252951786882901No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC260460.10103222693748186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT820550.075.925821
CGCGCGT10300.071.529581
GCGTGTT1083800.057.2600062
GAGCGGC41600.055.214569
ACCACCG117300.053.6514171
CCACCGA124600.050.3554272
GCGCGTT12900.049.3748172
CGGCGAC23600.046.9283371
ACCGAGA137450.046.2413254
CACCGAG137400.045.598563
CGTGTTT1433250.045.0003973
TACGGCG11900.040.742122
CCGAGAT250200.040.731341
CACGCGT19050.038.92431
GGCGACC27650.038.851132
AGAGCGG58650.038.5893068
CCGCGTG15700.037.84441
CGAGATC268850.037.7112732
ATCGGGA42250.037.1259542
CGGAGAT104850.036.90019642-43
Produced by FastQC (version 0.11.5)