##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233054_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25779893
Sequences flagged as poor quality	0
Sequence length	101
%GC	30
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4478449542052	34.0	31.0	34.0	31.0	34.0
2	32.775951630210415	34.0	33.0	34.0	31.0	34.0
3	32.817909872628256	34.0	33.0	34.0	31.0	34.0
4	36.10529919577246	37.0	37.0	37.0	35.0	37.0
5	36.083510703477316	37.0	37.0	37.0	35.0	37.0
6	36.129580289569084	37.0	37.0	37.0	35.0	37.0
7	36.07552731114904	37.0	37.0	37.0	35.0	37.0
8	35.82659784507251	37.0	37.0	37.0	35.0	37.0
9	37.59623474775477	39.0	39.0	39.0	37.0	39.0
10-11	37.700507368281166	39.0	39.0	39.0	37.0	39.0
12-13	37.824458871881276	39.0	39.0	39.0	37.0	39.0
14-15	39.39693161255556	41.0	40.0	41.0	38.0	41.0
16-17	39.16950947003543	41.0	39.5	41.0	37.5	41.0
18-19	38.69071935248141	41.0	39.0	41.0	36.0	41.0
20-21	38.292007651079075	41.0	39.0	41.0	35.0	41.0
22-23	37.86938652150341	41.0	38.0	41.0	35.0	41.0
24-25	37.306685950946346	41.0	37.0	41.0	33.0	41.0
26-27	36.84920701571569	41.0	37.0	41.0	31.0	41.0
28-29	36.22426582608392	41.0	37.0	41.0	25.5	41.0
30-31	35.61962499223716	41.0	36.0	41.0	17.5	41.0
32-33	35.2345588866486	41.0	36.0	41.0	11.5	41.0
34-35	34.920941913917176	41.0	35.5	41.0	7.5	41.0
36-37	34.67375717967488	40.0	35.0	41.0	6.0	41.0
38-39	34.50381388316856	40.0	35.0	41.0	2.0	41.0
40-41	34.357721752375	40.0	35.0	41.0	2.0	41.0
42-43	34.3014229539277	40.0	35.0	41.0	2.0	41.0
44-45	34.26328932009144	40.0	35.0	41.0	2.0	41.0
46-47	34.05842981970484	40.0	35.0	41.0	2.0	41.0
48-49	33.8994708395415	40.0	35.0	41.0	2.0	41.0
50-51	33.22123916495697	39.5	35.0	40.5	2.0	41.0
52-53	33.330199198266655	39.5	35.0	40.5	2.0	41.0
54-55	33.468822058338255	40.0	35.0	41.0	2.0	41.0
56-57	33.37851937554589	40.0	35.0	41.0	2.0	41.0
58-59	33.146203263915794	40.0	35.0	41.0	2.0	41.0
60-61	32.97114625339989	39.5	35.0	41.0	2.0	41.0
62-63	32.723066771456345	39.0	35.0	41.0	2.0	41.0
64-65	32.37970429124745	39.0	34.0	41.0	2.0	41.0
66-67	31.77880309666142	38.5	34.0	41.0	2.0	41.0
68-69	31.38886171870457	38.0	33.5	41.0	2.0	41.0
70-71	30.97408115696989	37.0	32.5	40.5	2.0	41.0
72-73	30.4945701093484	36.5	31.5	40.0	2.0	41.0
74-75	30.13280563266884	36.0	31.0	39.0	2.0	41.0
76-77	29.746205366329487	35.5	31.0	39.0	2.0	41.0
78-79	29.26378022593034	35.0	29.5	38.5	2.0	40.5
80-81	28.86599859045187	35.0	29.0	37.0	2.0	39.5
82-83	28.44473987925396	35.0	29.0	37.0	2.0	39.0
84-85	27.98632571904003	35.0	27.5	36.5	2.0	39.0
86-87	27.542877970827885	35.0	26.0	36.0	2.0	37.0
88-89	27.071164453630587	35.0	25.0	36.0	2.0	37.0
90-91	26.631481810261974	34.5	22.0	35.0	2.0	36.5
92-93	26.062155863098425	34.0	15.5	35.0	2.0	36.0
94-95	25.552738892283223	34.0	4.5	35.0	2.0	36.0
96-97	24.956407635206244	34.0	2.0	35.0	2.0	35.5
98-99	24.275324280826148	33.5	2.0	35.0	2.0	35.0
100-101	22.726401482736954	31.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.011622328866636167
1101	2	0.07480479243388061
1101	3	0.1819041365369003
1101	4	0.055696104980945904
1101	5	0.0020697054012615013
1101	6	-0.0021864798684205766
1101	7	-0.04045270860696348
1101	8	-0.07001350024679454
1101	9	-0.08351328210006415
1101	10-11	-0.06584033230740971
1101	12-13	-0.0025848397296712733
1101	14-15	0.05600144137253693
1101	16-17	0.06105484131732197
1101	18-19	0.06523082266327407
1101	20-21	0.10664677001710743
1101	22-23	0.06399557900055441
1101	24-25	0.1310326414323839
1101	26-27	0.05139277962596367
1101	28-29	0.04560007893009299
1101	30-31	0.07661723513201935
1101	32-33	0.06868269876175503
1101	34-35	0.010570163766310259
1101	36-37	0.0313892287154971
1101	38-39	0.03405132992160276
1101	40-41	-0.005432306861926861
1101	42-43	0.03144340690366931
1101	44-45	0.06859820839472519
1101	46-47	0.045942004710568085
1101	48-49	0.03342890029455958
1101	50-51	-0.06710656642979984
1101	52-53	-0.003189484087521066
1101	54-55	0.06648910145656117
1101	56-57	0.02719312969792753
1101	58-59	0.008794086951745328
1101	60-61	-0.07155148395512612
1101	62-63	-0.06007978517246926
1101	64-65	-0.019408808043102965
1101	66-67	0.08654839006088366
1101	68-69	0.19218373158468083
1101	70-71	0.19753737096318247
1101	72-73	0.3203484255693958
1101	74-75	0.24812355658835372
1101	76-77	0.2077230773837222
1101	78-79	0.23129803880161504
1101	80-81	0.20919512361531645
1101	82-83	0.20301550258367485
1101	84-85	0.20778942080053397
1101	86-87	0.2101104579692823
1101	88-89	0.23261631632977497
1101	90-91	0.2697239669313909
1101	92-93	0.3997496079909375
1101	94-95	0.47698167915581635
1101	96-97	0.45232266708333313
1101	98-99	0.525143640131855
1101	100-101	0.5852616674987026
1102	1	0.0602808376592705
1102	2	0.09041104274666623
1102	3	0.1693320435879997
1102	4	0.03436444423037699
1102	5	0.002812982363955996
1102	6	0.01791177046674619
1102	7	-0.025597195535866035
1102	8	-0.032969453778761704
1102	9	0.006093077015549397
1102	10-11	0.009562064964818262
1102	12-13	-0.007363961426932519
1102	14-15	0.047245054341708226
1102	16-17	0.06388968265615347
1102	18-19	0.09968127186269982
1102	20-21	0.09440759447461033
1102	22-23	0.06137428323560101
1102	24-25	0.12911655490270135
1102	26-27	0.024668501396860165
1102	28-29	-0.012755103515232236
1102	30-31	0.04803688664755157
1102	32-33	0.10617199829249557
1102	34-35	0.08015195917648299
1102	36-37	0.06560580160706309
1102	38-39	0.039586197858234584
1102	40-41	0.05471492105633047
1102	42-43	0.09564134198457452
1102	44-45	0.07444863254195155
1102	46-47	0.01290890781649523
1102	48-49	0.0017862219044744165
1102	50-51	0.046047751515899904
1102	52-53	-7.851422745730474E-4
1102	54-55	0.056848713938698836
1102	56-57	0.060485128238731534
1102	58-59	0.05757415440803726
1102	60-61	-0.022187368023843135
1102	62-63	-0.024677519159226335
1102	64-65	-0.0026582719407599598
1102	66-67	0.0949326170785767
1102	68-69	0.1861583797783659
1102	70-71	0.1726871480584471
1102	72-73	0.28205111035989816
1102	74-75	0.25069168230956507
1102	76-77	0.190298421727217
1102	78-79	0.2472773063391429
1102	80-81	0.21271902307838886
1102	82-83	0.2073625216792898
1102	84-85	0.251274666586621
1102	86-87	0.27857068001302565
1102	88-89	0.2977751741532657
1102	90-91	0.29027268728468414
1102	92-93	0.40020517051733506
1102	94-95	0.42545921084803595
1102	96-97	0.4886584799229219
1102	98-99	0.5795065589061466
1102	100-101	0.6544449782303907
1103	1	0.060324778461577466
1103	2	0.05190832150674396
1103	3	0.1734334556115229
1103	4	0.05875039372558177
1103	5	0.025423303213251813
1103	6	0.010681174572795271
1103	7	-0.011909975135054651
1103	8	-0.019897507106023227
1103	9	-0.0020000893406830755
1103	10-11	-0.02346489519224093
1103	12-13	-6.435597158400697E-4
1103	14-15	0.044304800119753907
1103	16-17	0.04665780740112524
1103	18-19	0.01462127191215501
1103	20-21	0.06913632685618154
1103	22-23	0.051534893850558205
1103	24-25	0.06112123180300699
1103	26-27	0.043722993214743155
1103	28-29	0.05844155836064857
1103	30-31	0.06133982681679129
1103	32-33	0.07165604752714927
1103	34-35	0.07005759868442141
1103	36-37	0.05667075612755923
1103	38-39	0.02109910355458311
1103	40-41	0.050564908942391185
1103	42-43	0.07564155919024529
1103	44-45	0.069957375619218
1103	46-47	0.10019677776391944
1103	48-49	0.07646897296324084
1103	50-51	0.0701702483768969
1103	52-53	0.06987523169679122
1103	54-55	0.038957816332903406
1103	56-57	0.06570524558591018
1103	58-59	0.08631508839321356
1103	60-61	0.07098165523686362
1103	62-63	0.0471931379950945
1103	64-65	0.09574433191713894
1103	66-67	0.13024678162232917
1103	68-69	0.2273190668679952
1103	70-71	0.20525362662952418
1103	72-73	0.32866862456922163
1103	74-75	0.3121783578794428
1103	76-77	0.18848918300120943
1103	78-79	0.2545979148961486
1103	80-81	0.26642349386348485
1103	82-83	0.2264383603822182
1103	84-85	0.1815146840266273
1103	86-87	0.18223000759199692
1103	88-89	0.242282736790699
1103	90-91	0.244163518063214
1103	92-93	0.36269447193130944
1103	94-95	0.3785496028352249
1103	96-97	0.43242233946331154
1103	98-99	0.5417867411349597
1103	100-101	0.5422861529470175
1104	1	0.06409001089213007
1104	2	0.08037332726600965
1104	3	0.15114930404954663
1104	4	0.05771671828501468
1104	5	0.020634810595659303
1104	6	0.009998191294158687
1104	7	-0.028639309945901914
1104	8	-0.017823381889925827
1104	9	-0.06787546407836942
1104	10-11	-0.03951246657131691
1104	12-13	-0.008460605098996155
1104	14-15	-0.024949576435524534
1104	16-17	-0.0034028929853846535
1104	18-19	-0.0020982683120891465
1104	20-21	-0.021711048835157953
1104	22-23	-0.028257599838163117
1104	24-25	-0.010441422245513365
1104	26-27	-0.019167507501755665
1104	28-29	-0.034500617324347616
1104	30-31	-0.02640529922713597
1104	32-33	-0.06583844557841445
1104	34-35	-0.06786671250994658
1104	36-37	-0.04849617195202427
1104	38-39	-0.07796904181336117
1104	40-41	-0.10808152178707786
1104	42-43	-0.07378092770971989
1104	44-45	-0.054631533785901354
1104	46-47	-0.0944054406023298
1104	48-49	0.010965535286395323
1104	50-51	-0.028475239184409418
1104	52-53	-0.02551442481547639
1104	54-55	-0.0022489395681191127
1104	56-57	-0.008432615913299912
1104	58-59	-0.00477027837870736
1104	60-61	-0.04899397911898262
1104	62-63	-0.04154680806169608
1104	64-65	-0.025338613983642233
1104	66-67	-0.009747080515865036
1104	68-69	0.029781612896190524
1104	70-71	0.02659489136340909
1104	72-73	0.17359333821729095
1104	74-75	0.14875247932550195
1104	76-77	0.08309161791534336
1104	78-79	0.06289455651053899
1104	80-81	0.05041621054152401
1104	82-83	0.04654446846236837
1104	84-85	0.019738789870856976
1104	86-87	-0.0034315388802923508
1104	88-89	0.014948566282662057
1104	90-91	0.026706933877484573
1104	92-93	0.08407119682174269
1104	94-95	0.13409203295306327
1104	96-97	0.16144612312406892
1104	98-99	0.20488189855460703
1104	100-101	0.16441369151879925
1105	1	0.08896708205311654
1105	2	0.0982324518120734
1105	3	0.15766395125562838
1105	4	0.04722614428428784
1105	5	0.03600559694793759
1105	6	0.007843486731360372
1105	7	0.011680214048560345
1105	8	0.08347001679657495
1105	9	0.07386398334791977
1105	10-11	0.08154054446227121
1105	12-13	0.021453896371895098
1105	14-15	0.016488862483022615
1105	16-17	0.029425623776511145
1105	18-19	0.029488489023435704
1105	20-21	0.01582886682399476
1105	22-23	0.013565283090748892
1105	24-25	0.010342224422821289
1105	26-27	0.01965438122137897
1105	28-29	0.03030604667262793
1105	30-31	0.016547636395699783
1105	32-33	0.01968259175493614
1105	34-35	0.024489335081803176
1105	36-37	0.051877016239700424
1105	38-39	0.04231291969582429
1105	40-41	0.016738345124409193
1105	42-43	-0.008767608727211496
1105	44-45	0.015960088106417913
1105	46-47	0.013640072094148081
1105	48-49	0.05338110307911137
1105	50-51	0.0038196839007724748
1105	52-53	-0.010106585779055877
1105	54-55	0.050264540361986576
1105	56-57	0.012066519899306627
1105	58-59	0.010711000827541284
1105	60-61	0.036569557699955624
1105	62-63	0.038004414552730736
1105	64-65	0.08560338372036114
1105	66-67	0.029180106774354897
1105	68-69	0.06764327435088902
1105	70-71	0.11548657898613612
1105	72-73	0.27011978758820376
1105	74-75	0.21409019915839878
1105	76-77	0.1416998425725069
1105	78-79	0.2164985443822509
1105	80-81	0.1544171943966397
1105	82-83	0.11230619761388638
1105	84-85	0.10813667503173363
1105	86-87	0.12802515593237374
1105	88-89	0.1391206278128223
1105	90-91	0.16400077031708094
1105	92-93	0.25840706970514304
1105	94-95	0.2576363128796011
1105	96-97	0.33167997175968367
1105	98-99	0.3611463011119156
1105	100-101	0.3501496987661703
1106	1	0.05063607569253037
1106	2	0.060457161399597226
1106	3	0.1158845226482228
1106	4	0.03766495836680406
1106	5	-0.013445646897771724
1106	6	-0.022984942256591978
1106	7	-0.012891876328993135
1106	8	0.11532390813689375
1106	9	0.09386505952437574
1106	10-11	0.06951454287395364
1106	12-13	-0.0011906054083752338
1106	14-15	-0.026250821011757353
1106	16-17	-0.009028215339149881
1106	18-19	-0.014753596442133698
1106	20-21	-0.011085559006161816
1106	22-23	-0.02185489906478466
1106	24-25	-0.05876017631768349
1106	26-27	-0.06908026727450078
1106	28-29	-0.07729930273435315
1106	30-31	-0.10082980401480768
1106	32-33	-0.10458541989526537
1106	34-35	-0.08705217551991495
1106	36-37	-0.04272477576375877
1106	38-39	-0.05790359622599084
1106	40-41	-0.11078383732168362
1106	42-43	-0.11058633637932758
1106	44-45	-0.11154607512457915
1106	46-47	-0.09547745289184206
1106	48-49	-0.10839870871389934
1106	50-51	-0.14525677724992647
1106	52-53	-0.18848500489703923
1106	54-55	-0.14008395513020133
1106	56-57	-0.16156111083537184
1106	58-59	-0.12717162631297896
1106	60-61	-0.08723627363001896
1106	62-63	-0.1146434249146111
1106	64-65	-0.07292801094114765
1106	66-67	-0.0921361411976136
1106	68-69	-0.05707711577911212
1106	70-71	-0.019256011376974413
1106	72-73	0.1423120789799981
1106	74-75	0.08787229503197125
1106	76-77	0.02388455097898401
1106	78-79	0.0684700160767413
1106	80-81	-0.02147647272701292
1106	82-83	-0.018425185532564115
1106	84-85	0.01353073070027122
1106	86-87	-0.006349799953891733
1106	88-89	-0.0075192843919680286
1106	90-91	0.009290245311532885
1106	92-93	0.07536064443441859
1106	94-95	0.06309485622361777
1106	96-97	0.06123171658284576
1106	98-99	0.10389649506078769
1106	100-101	0.0826478383217939
1107	1	0.009117156892756384
1107	2	-0.029407357946169554
1107	3	0.008379069687300955
1107	4	-0.06198736526118154
1107	5	-0.07827418261992136
1107	6	-0.1335309467636776
1107	7	-0.07516586227988142
1107	8	0.061183442143821765
1107	9	0.035453563967905666
1107	10-11	-0.04029601492312196
1107	12-13	-0.10557034497880835
1107	14-15	-0.1722520652832671
1107	16-17	-0.17990860466823477
1107	18-19	-0.1969860564338788
1107	20-21	-0.2545395579447529
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2308	12-13	-0.26408549298253803
2308	14-15	-0.35479236923223567
2308	16-17	-0.3890715907342681
2308	18-19	-0.48061105922463554
2308	20-21	-0.47883709826328413
2308	22-23	-0.544692588493767
2308	24-25	-0.4800624024911073
2308	26-27	-0.4513048473970187
2308	28-29	-0.5349353688555638
2308	30-31	-0.5448346032395577
2308	32-33	-0.5614554032371828
2308	34-35	-0.5542054186478893
2308	36-37	-0.5048477400230098
2308	38-39	-0.469315012576331
2308	40-41	-0.47393343181195746
2308	42-43	-0.49244686573439367
2308	44-45	-0.5760583572803384
2308	46-47	-0.535121164334754
2308	48-49	-0.7934524272172396
2308	50-51	-0.6204548853677423
2308	52-53	-0.46561760437558775
2308	54-55	-0.4868679532426228
2308	56-57	-0.5667451832339268
2308	58-59	-0.5257653256771064
2308	60-61	-0.5160428021619268
2308	62-63	-0.5232787485802248
2308	64-65	-0.6129988828582498
2308	66-67	-0.5150012857061199
2308	68-69	-0.5909042201132877
2308	70-71	-0.6223588646756149
2308	72-73	-0.4638515519023443
2308	74-75	-0.5653312146982579
2308	76-77	-0.6273033161614023
2308	78-79	-0.6759637657226101
2308	80-81	-0.7961359914899937
2308	82-83	-0.9376353245378048
2308	84-85	-0.9949508479958915
2308	86-87	-1.027854691013669
2308	88-89	-1.1507478559993167
2308	90-91	-1.269478525193982
2308	92-93	-1.410583647001065
2308	94-95	-1.5980167334947488
2308	96-97	-1.944626241029912
2308	98-99	-2.367154158034161
2308	100-101	-2.813378293331514
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	136237.0
3	38515.0
4	26303.0
5	26409.0
6	43544.0
7	81179.0
8	151227.0
9	297709.0
10	503122.0
11	557197.0
12	467398.0
13	366545.0
14	268045.0
15	216658.0
16	188187.0
17	175771.0
18	177599.0
19	172324.0
20	175016.0
21	182598.0
22	190363.0
23	199059.0
24	248361.0
25	339695.0
26	205339.0
27	220867.0
28	217067.0
29	232115.0
30	264677.0
31	306857.0
32	369619.0
33	457227.0
34	610443.0
35	1208624.0
36	1730832.0
37	3602931.0
38	7415046.0
39	3701951.0
40	7237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.731811503509824	16.667044986357173	10.179548970374459	40.42159453975855
2	17.300805820100308	19.198026873314472	50.64627058099796	12.854896725587265
3	19.063209458944705	21.49937156377384	39.89789155137303	19.539527425908425
4	20.345992418653818	27.321662440668128	36.04172984940861	16.290615291269443
5	20.00259191133254	29.79506219518569	37.55952701309809	12.64281888038368
6	17.73813676955142	32.3172328900832	38.08000039185998	11.864629948505401
7	12.036981705063694	19.863518046551544	56.11679110917471	11.982709139210044
8	11.9742224001264	23.833269729479902	49.53568020666743	14.656827663726272
9	14.705281839211631	21.42055166984155	49.61431988562485	14.259846605321966
10-11	15.740684783450732	32.01443405116641	40.433803986426724	11.811077178956133
12-13	15.455959816135609	25.133550358621438	45.42727966879239	13.983210156450568
14-15	14.88687355673424	26.767353454899943	45.142879323652004	13.202893664713816
16-17	16.626229281500663	26.861706943432946	43.190474583592696	13.321589191473695
18-19	16.352534042545535	27.563755524470572	42.7478983840726	13.335812048911288
20-21	16.528781340203558	28.771858591766904	41.3076074287524	13.391752639277147
22-23	17.442207032241924	28.44506401510067	41.03195821286435	13.080770739793051
24-25	15.803227735771072	30.716712941576912	39.85936252461647	13.620696798035544
26-27	16.448177270993217	30.75721477797812	38.998812704949565	13.795795246079095
28-29	16.947534595456624	31.11082596326477	37.95318979519488	13.988449646083726
30-31	17.08885171907082	30.717109311664075	38.25808518708535	13.935953782179752
32-33	15.890597276081762	31.854507833256474	38.05564527823261	14.199249612429151
34-35	16.848362317369276	32.75533521277292	36.17217683886157	14.224125630996237
36-37	15.503076136144422	32.20287587890924	37.30788129058881	14.986166694357525
38-39	15.49534541664416	31.56656596970829	37.4223530208786	15.51573559276895
40-41	17.096091227640482	31.311037489791183	37.0205881819757	14.572283100592639
42-43	16.27683057669448	31.388826119798356	38.164108459619804	14.170234843887355
44-45	17.212333839277743	30.512318802944478	37.849703192896314	14.425644164881469
46-47	15.91862240523399	31.578785513036888	36.98416484317108	15.518427238558038
48-49	15.19143079001856	31.340466242732244	37.139380189344315	16.32872277790488
50-51	16.367904849988225	31.421930623871557	37.70938582590123	14.500778700238989
52-53	15.272766249343576	33.05370104209953	37.432824606172126	14.240708102384769
54-55	15.451185987118937	33.339139809354194	35.68893663346859	15.520737570058282
56-57	15.647521092852635	32.69742482033768	37.10748484702785	14.547569239781835
58-59	15.190897061207645	35.17682352399758	35.0208656515591	14.611413763235678
60-61	14.97184646055377	35.63888352529981	34.59374356822025	14.795526445926175
62-63	14.85167158831692	36.575757680415116	34.10255227182954	14.470018459438421
64-65	14.936624691335748	35.93897040868685	34.45594423830447	14.668460661672933
66-67	15.210147906740431	36.63500736611473	33.7551266117184	14.399718115426447
68-69	15.626909719294682	36.177425427550574	33.88458732327398	14.31107752988076
70-71	14.974654485938965	36.630001945945935	33.54479576658094	14.850547801534159
72-73	15.412610759786272	35.976967013489244	33.726483353100306	14.883938873624174
74-75	15.536275223054494	35.776763798421044	33.724475228817184	14.962485749707282
76-77	15.38062401766955	35.76773099914556	33.81060820259072	15.041036780594169
78-79	15.459403210565902	36.18721948893928	33.46703609543854	14.886341205056288
80-81	15.56056516486019	36.25745742875634	33.49613248364454	14.685844922738935
82-83	15.690969719852982	35.86421060753739	33.33306811022558	15.111751562384047
84-85	15.610766046352234	35.88285320484772	33.37729796701864	15.129082781781399
86-87	16.259703321129315	35.993898863542526	32.889932591155834	14.856465224172325
88-89	16.21243856643729	35.90480707130259	33.04435609820784	14.838398264052277
90-91	15.835220368348924	36.36952279498706	32.65492260536363	15.14033423130038
92-93	16.527779882872792	36.009453889534086	32.45051114607219	15.012255081520934
94-95	16.351207614692044	36.50014868139341	31.639854655932314	15.508789047982226
96-97	16.133525928884424	36.812757107006775	32.19632675026835	14.857390213840446
98-99	16.401123493657987	36.60673237295042	31.773550556700357	15.218593576691239
100-101	17.046021091047333	36.86220142500363	30.724724698888878	15.367052785060157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130890.0
1	143881.5
2	132939.5
3	127275.5
4	165202.0
5	207655.0
6	216160.0
7	201760.0
8	202683.5
9	204623.5
10	207400.0
11	209530.0
12	214584.5
13	217837.5
14	232740.5
15	243578.5
16	237997.5
17	242213.5
18	255314.0
19	274917.0
20	300286.5
21	338338.5
22	391409.0
23	446237.5
24	507345.0
25	598231.5
26	697302.0
27	784490.5
28	861092.5
29	934209.0
30	992517.0
31	998054.5
32	985545.5
33	988110.0
34	994293.5
35	983044.0
36	968246.0
37	948343.5
38	924545.0
39	894453.0
40	850602.0
41	786909.5
42	680132.5
43	581687.0
44	505483.5
45	439301.5
46	388211.0
47	346818.0
48	307916.0
49	263715.0
50	213958.0
51	167157.0
52	131731.0
53	107287.0
54	97409.0
55	105771.5
56	88451.5
57	52838.0
58	36316.5
59	25289.5
60	17713.5
61	12731.0
62	9181.0
63	6656.0
64	4708.0
65	3273.0
66	2282.0
67	1592.5
68	1133.0
69	768.5
70	560.0
71	472.0
72	398.0
73	326.0
74	237.0
75	187.0
76	173.0
77	148.5
78	118.5
79	93.0
80	73.0
81	51.0
82	40.0
83	33.0
84	21.5
85	21.5
86	22.5
87	14.5
88	9.0
89	8.5
90	6.0
91	6.0
92	5.5
93	3.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20206833286701387
2	0.19618778091902864
3	0.20380612130546857
4	0.1720643293593189
5	0.17838708640101802
6	0.2188488524758423
7	0.2316378892650951
8	0.22568363646815756
9	0.25839905541888786
10-11	0.25538119960389283
12-13	0.26253793993636826
14-15	0.26805774562369206
16-17	0.2794445267868257
18-19	0.28850197322386095
20-21	0.28021256721274984
22-23	0.28812571099499906
24-25	0.2832362415158201
26-27	0.25757476960823694
28-29	0.22631397267630243
30-31	0.22849202671244603
32-33	0.2755829902009291
34-35	0.2932498594932105
36-37	0.2849352400337736
38-39	0.2866051461113512
40-41	0.28772423531781144
42-43	0.29095737519158826
44-45	0.2928173518796218
46-47	0.28970252126337376
48-49	0.058718242158724246
50-51	0.06380554023245946
52-53	0.04472089934585841
54-55	0.049210832643874824
56-57	0.042110337696126196
58-59	0.034255378794628824
60-61	0.0365439841042009
62-63	0.04041909716227294
64-65	0.04108046530681877
66-67	0.04615612640440362
68-69	0.04985862431624523
70-71	0.04241483857206079
72-73	0.048826812430912726
74-75	0.041448969551580374
76-77	0.041951299022071196
78-79	0.03605911009793563
80-81	0.05030664789803433
82-83	0.05981793640493387
84-85	0.059133294308087316
86-87	0.06144517356996012
88-89	0.061999869433127595
90-91	0.06693200782485792
92-93	0.06083229282604082
94-95	0.06497311683954624
96-97	0.0622888543408617
98-99	0.055746934248330667
100-101	0.05700372767257025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.5779893E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.15324686868472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.05005161116391	33.35472782673866
2	9.51110839019634	7.828259831531368
3	3.024566737464295	3.7341222485304137
4	1.6152423985522517	2.65889876721549
5	0.9544544381665606	1.963944955939012
6	0.6122393629146421	1.5117382586851513
7	0.45581269047209494	1.3130720523814245
8	0.34946727517075155	1.150537043810281
9	0.259405397017767	0.9607836908287202
>10	1.8225952745172782	14.559686781205446
>50	0.1915515665134951	5.493518461417616
>100	0.13363692952570555	10.775218041583452
>500	0.012286093886189448	3.4524656625709937
>1k	0.006952690118994031	5.261124062455144
>5k	3.380476941290055E-4	0.9118965598674367
>10k+	2.910966254984025E-4	5.070005755239294
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	264545	1.0261679518995677	Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163891	0.6357318860865715	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123613	0.47949384429175096	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	122171	0.4739003377554748	Illumina Single End PCR Primer 1 (100% over 50bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80191	0.3110602514913464	No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	54992	0.213313530820318	Illumina Single End PCR Primer 1 (97% over 44bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	52898	0.2051909214673622	Illumina Single End PCR Primer 1 (100% over 43bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	47261	0.18332504328082355	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	29987	0.11631933460701331	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	26849	0.10414705755372995	Illumina Single End PCR Primer 1 (98% over 50bp)
GCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26432	0.10252951786882901	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	26046	0.10103222693748186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.544843223360159	0.0	0.0	1.163697615036649E-5	0.0
2	0.5481093346663619	3.878992050122163E-6	0.0	4.654790460146596E-5	0.0
3	0.5482877683006675	3.878992050122163E-6	0.0	5.042689665158812E-5	0.0
4	0.8637118858484013	3.878992050122163E-6	0.0	6.594286485207677E-5	0.0
5	0.8669508442102534	3.878992050122163E-6	0.0	6.594286485207677E-5	0.0
6	0.8684403771575002	3.878992050122163E-6	0.0	6.982185690219893E-5	0.0
7	0.8855118211700879	3.878992050122163E-6	0.0	7.757984100244326E-5	0.0
8	1.1240969851969518	3.878992050122163E-6	0.0	7.757984100244326E-5	0.0
9	1.12530723071659	3.878992050122163E-6	0.0	1.047327853532984E-4	0.0
10-11	1.1823187163732602	3.878992050122163E-6	0.0	1.1249076945354273E-4	0.0
12-13	1.2224216756834485	3.878992050122163E-6	0.0	1.1830925752872597E-4	0.0
14-15	1.2419853720882394	3.878992050122163E-6	0.0	1.357647217542757E-4	0.0
16-17	1.2577049873713595	3.878992050122163E-6	0.0	1.6097817008006975E-4	0.0
18-19	1.2652030790042457	7.757984100244325E-6	0.0	1.7455464225549734E-4	0.0
20-21	1.2700227266265225	7.757984100244325E-6	0.0	1.8813111443092493E-4	0.0
22-23	1.273535153927908	7.757984100244325E-6	0.0	2.0170758660635247E-4	0.0
24-25	1.2765336147826525	7.757984100244325E-6	0.0	2.2692103493214652E-4	0.0
26-27	1.2803835143923987	7.757984100244325E-6	0.0	3.1225886003483414E-4	3.878992050122163E-6
28-29	1.2840937702883406	7.757984100244325E-6	0.0	3.723832368117277E-4	3.878992050122163E-6
30-31	1.2884169069282017	1.163697615036649E-5	0.0	3.859597089871552E-4	3.878992050122163E-6
32-33	1.293190006645877	1.163697615036649E-5	0.0	3.9371769308739956E-4	3.878992050122163E-6
34-35	1.2977769147451466	1.163697615036649E-5	0.0	3.956571891124606E-4	3.878992050122163E-6
36-37	1.3035197624753523	1.9394960250610815E-5	0.0	4.3250761358862115E-4	3.878992050122163E-6
38-39	1.3102304187220637	1.9394960250610815E-5	0.0	4.3444710961368225E-4	3.878992050122163E-6
40-41	1.3185392196934256	1.9394960250610815E-5	0.0	4.480235817891098E-4	3.878992050122163E-6
42-43	1.327036151779218	1.9394960250610815E-5	0.0	4.6160005396453743E-4	3.878992050122163E-6
44-45	1.3404787987289164	1.9394960250610815E-5	0.0	4.8681350229033146E-4	3.878992050122163E-6
46-47	1.4366079797150437	1.9394960250610815E-5	0.0	5.139664466411865E-4	3.878992050122163E-6
48-49	1.4572500359097689	2.1334456275671897E-5	0.0	5.159059426662477E-4	3.878992050122163E-6
50-51	1.4700390726990218	2.327395230073298E-5	0.0	5.197849347163699E-4	3.878992050122163E-6
52-53	1.4844631046374008	2.5213448325794062E-5	0.0	5.217244307414309E-4	3.878992050122163E-6
54-55	1.5003592140588016	2.715294435085514E-5	0.0	5.256034227915531E-4	3.878992050122163E-6
56-57	1.5169593605372993	3.2971432426038384E-5	0.0	5.411193909920418E-4	3.878992050122163E-6
58-59	1.5383636386698734	3.4910928451099466E-5	0.0	5.488773750922861E-4	3.878992050122163E-6
60-61	1.5641085089065343	3.4910928451099466E-5	0.0	5.527563671424082E-4	3.878992050122163E-6
62-63	1.5875996847620741	3.4910928451099466E-5	0.0	5.663328393178358E-4	3.878992050122163E-6
64-65	1.6157747435181364	3.685042447616055E-5	0.0	5.876672955935076E-4	3.878992050122163E-6
66-67	1.6434067433871817	4.2668912551343795E-5	0.0	5.93485783668691E-4	3.878992050122163E-6
68-69	1.6742020612731014	4.654790460146596E-5	0.0	5.93485783668691E-4	3.878992050122163E-6
70-71	1.7135137062050645	5.042689665158812E-5	0.0	6.012437677689352E-4	3.878992050122163E-6
72-73	1.754999526181121	5.042689665158812E-5	0.0	6.109412478942408E-4	3.878992050122163E-6
74-75	1.8028604695915535	5.042689665158812E-5	0.0	6.303362081448514E-4	3.878992050122163E-6
76-77	1.86102789487916	5.042689665158812E-5	0.0	6.807631047964396E-4	3.878992050122163E-6
78-79	1.9250157477379755	5.042689665158812E-5	0.0	6.962790729969283E-4	3.878992050122163E-6
80-81	1.9979136453359212	5.8184880751832446E-5	0.0	7.156740332475391E-4	3.878992050122163E-6
82-83	2.0832495309425836	6.20638728019546E-5	0.0	7.253715133728445E-4	3.878992050122163E-6
84-85	2.182889199734072	6.20638728019546E-5	0.0	7.331294974730889E-4	3.878992050122163E-6
86-87	2.3025832574246916	6.400336882701569E-5	0.0	7.525244577236996E-4	3.878992050122163E-6
88-89	2.444482605106235	6.982185690219893E-5	0.0	7.62221937849005E-4	3.878992050122163E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	82055	0.0	75.92582	1
CGCGCGT	1030	0.0	71.52958	1
GCGTGTT	108380	0.0	57.260006	2
GAGCGGC	4160	0.0	55.21456	9
ACCACCG	11730	0.0	53.651417	1
CCACCGA	12460	0.0	50.355427	2
GCGCGTT	1290	0.0	49.374817	2
CGGCGAC	2360	0.0	46.928337	1
ACCGAGA	13745	0.0	46.241325	4
CACCGAG	13740	0.0	45.59856	3
CGTGTTT	143325	0.0	45.000397	3
TACGGCG	1190	0.0	40.74212	2
CCGAGAT	25020	0.0	40.73134	1
CACGCGT	1905	0.0	38.9243	1
GGCGACC	2765	0.0	38.85113	2
AGAGCGG	5865	0.0	38.589306	8
CCGCGTG	1570	0.0	37.8444	1
CGAGATC	26885	0.0	37.711273	2
ATCGGGA	4225	0.0	37.125954	2
CGGAGAT	10485	0.0	36.900196	42-43
>>END_MODULE