SRR1233054_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1233054_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25779893
Sequences flagged as poor quality	0
Sequence length	101
%GC	31

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	398662	1.5464067286858019	TruSeq Adapter, Index 3 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146143	0.5668875351810033	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG	128002	0.49651874039973715	TruSeq Adapter, Index 3 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118449	0.4594627293449201	No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69942	0.27130446196964436	No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC	56068	0.21748732626624945	TruSeq Adapter, Index 3 (100% over 42bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	37496	0.14544668591138063	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	29850	0.11578791269614658	No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	25959	0.10069475462912124	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	10790	86.02187	1
GTCGCCG	1915	78.61972	6
CGCGTGT	80910	78.43023	1
CGGAGAT	7890	73.81896	1
CGCCGTA	840	68.41434	8
GTGGTCG	4495	67.41093	3
GGTCGCC	2350	65.68327	5
GCGTGTT	107120	59.30585	2
GGTAGAT	10255	59.280743	5
CGCCGAG	1925	58.96688	8
TCGCCGA	1725	58.9204	7
CGCGCGT	1325	56.069572	1
GGTGGTA	11265	53.54444	2
CCGAGAT	9190	53.375282	1
TCGCCGT	990	52.77138	7
GTAGATC	12345	49.860302	6
GGTGGTC	6530	48.948994	2
TCGGCAC	1960	48.705833	7
GTGGTAG	12535	47.96768	3
CGGCACT	1940	46.025356	8