FastQCFastQC Report
Fri 27 May 2016
SRR1233053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33176924
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC10772333.246934525937365Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8250212.486731440202232Illumina Single End PCR Primer 1 (100% over 50bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC2753650.8299895433343971Illumina Single End PCR Primer 1 (97% over 44bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2621790.790245051048132No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2342920.7061896395217351Illumina Single End PCR Primer 1 (100% over 43bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1307490.3940962097631474No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1191320.35908090816375865No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1139480.3434555897948827No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG984130.29663087512272085Illumina Single End PCR Primer 1 (98% over 50bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT681210.20532644919100998No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635590.19157592789494288No Hit
GCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC573410.17283398545326262Illumina Single End PCR Primer 1 (100% over 40bp)
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT526810.158788078123216No Hit
CGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG512070.1543452310407077Illumina Single End PCR Primer 1 (100% over 41bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC508480.1532631536305174Illumina Single End PCR Primer 1 (100% over 47bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA432980.13050637244127875No Hit
CCGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC385500.11619522050929133Illumina Single End PCR Primer 1 (97% over 42bp)
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA378130.11397379696803718No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT335130.10101298119138472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT940800.063.3147931
GAGCGGC142400.053.044799
ACCACCG531800.051.442251
GCGTGTT1154400.051.122852
CCACCGA553200.049.3585662
ATCGGGA95500.048.3248562
CACCGAG570300.048.0084573
ACCGAGA574600.047.8000534
CCGAGAT1087800.044.207211
GATCCGG37000.043.5371065
GCGACCA201350.043.3143581
CGAGATC1133150.042.2960782
CGTGTTT1452600.041.959913
TCGGGAG98800.041.752843
TACGGCG50700.041.6226462
CGGAGAT475350.041.3734842-43
TCTCGGA491750.041.35769340-41
TCGGAGA490250.041.2515342-43
ATACGGC54600.040.5641
CTCGGAG493900.040.2913940-41
Produced by FastQC (version 0.11.5)