##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233053_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33176924
Sequences flagged as poor quality	0
Sequence length	101
%GC	34
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03748934048256	34.0	31.0	34.0	30.0	34.0
2	32.28212971160316	34.0	33.0	34.0	31.0	34.0
3	32.34795823747856	34.0	33.0	34.0	31.0	34.0
4	35.4790653588018	37.0	37.0	37.0	35.0	37.0
5	35.524580247403286	37.0	37.0	37.0	35.0	37.0
6	35.54737117883502	37.0	37.0	37.0	35.0	37.0
7	35.40204052672273	37.0	37.0	37.0	35.0	37.0
8	35.26941352368893	37.0	36.0	37.0	33.0	37.0
9	36.72396286647912	39.0	39.0	39.0	32.0	39.0
10-11	36.90273668830781	39.0	38.5	39.0	33.5	39.0
12-13	37.01425662608143	39.0	39.0	39.0	34.5	39.0
14-15	38.34642281785979	41.0	39.0	41.0	34.0	41.0
16-17	38.05929645557255	41.0	39.0	41.0	33.5	41.0
18-19	37.61383318718757	41.0	38.0	41.0	32.5	41.0
20-21	37.28691927557841	40.5	38.0	41.0	32.0	41.0
22-23	37.116499498265725	40.0	37.0	41.0	32.0	41.0
24-25	36.59245189517871	40.5	37.0	41.0	29.0	41.0
26-27	36.42518328401994	40.5	37.0	41.0	28.5	41.0
28-29	35.952399625715756	40.0	36.0	41.0	24.5	41.0
30-31	35.30478386121631	40.0	35.0	41.0	17.5	41.0
32-33	35.12643120561749	40.0	35.0	41.0	16.0	41.0
34-35	35.04312574306165	40.0	35.0	41.0	16.0	41.0
36-37	34.91199360434982	40.0	35.0	41.0	14.5	41.0
38-39	34.97596371502071	40.0	35.0	41.0	14.0	41.0
40-41	34.9490687412733	40.0	35.0	41.0	10.0	41.0
42-43	34.6862650829233	40.0	35.0	41.0	9.0	41.0
44-45	34.54744440744416	40.0	35.0	41.0	7.0	41.0
46-47	34.18544018728198	40.0	35.0	41.0	4.0	41.0
48-49	34.13379055273478	40.0	35.0	41.0	2.0	41.0
50-51	33.45931649661072	39.5	34.5	40.5	2.0	41.0
52-53	33.404352133428645	39.0	34.5	40.5	2.0	41.0
54-55	33.51875864079503	39.0	35.0	41.0	2.0	41.0
56-57	33.42589902246513	39.0	35.0	41.0	2.0	41.0
58-59	32.9776112909081	39.0	34.0	41.0	2.0	41.0
60-61	32.86831038043189	38.0	34.0	41.0	2.0	41.0
62-63	32.578487445068745	38.0	34.0	41.0	2.0	41.0
64-65	31.999739758875776	37.0	33.0	40.5	2.0	41.0
66-67	30.362041912022946	37.0	29.5	40.0	2.0	41.0
68-69	29.46639575447079	36.0	27.0	40.0	2.0	41.0
70-71	28.992043942952638	36.0	25.5	39.5	2.0	41.0
72-73	28.572260601977447	35.0	23.5	39.0	2.0	41.0
74-75	28.118977470605774	35.0	21.0	39.0	2.0	41.0
76-77	27.566311331333793	35.0	16.5	38.5	2.0	40.0
78-79	26.997242420665643	35.0	8.0	37.0	2.0	39.5
80-81	26.70001316276337	35.0	4.5	37.0	2.0	39.0
82-83	26.271512452450384	35.0	2.0	36.0	2.0	39.0
84-85	25.762842691504492	34.5	2.0	36.0	2.0	37.5
86-87	25.417570929722118	34.0	2.0	35.5	2.0	37.0
88-89	24.940001701182425	34.0	2.0	35.0	2.0	37.0
90-91	24.533390587988205	34.0	2.0	35.0	2.0	36.0
92-93	24.169418056960314	34.0	2.0	35.0	2.0	36.0
94-95	23.680384037411066	33.5	2.0	35.0	2.0	36.0
96-97	23.03297849432937	33.0	2.0	35.0	2.0	35.0
98-99	22.440190326264123	32.0	2.0	35.0	2.0	35.0
100-101	20.99837221196275	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0905125886417224
1101	2	0.08444380167003374
1101	3	0.07565338543952294
1101	4	0.14816982422838976
1101	5	0.08111503938064146
1101	6	0.05467963199696868
1101	7	-0.04884770975059638
1101	8	-0.06861533303096223
1101	9	0.08541379315867204
1101	10-11	0.033689531131081196
1101	12-13	-0.01144015911453522
1101	14-15	0.031620690947413266
1101	16-17	0.11161127330619536
1101	18-19	0.14785884463151433
1101	20-21	0.11636891854612941
1101	22-23	0.12809208489543522
1101	24-25	0.11818074342847495
1101	26-27	0.08066264259396405
1101	28-29	0.0799752340494706
1101	30-31	0.19363497545784014
1101	32-33	0.16831289353003598
1101	34-35	0.08869133212974134
1101	36-37	0.009350668147881436
1101	38-39	-0.040681443718582955
1101	40-41	-0.07330209984983327
1101	42-43	0.012185906417869319
1101	44-45	0.05175311779777303
1101	46-47	0.008348076854829856
1101	48-49	-0.030779094307945343
1101	50-51	-0.1420435699726852
1101	52-53	-0.09596853159070662
1101	54-55	-0.10799768971230606
1101	56-57	-0.13438202981249248
1101	58-59	-0.015473647455991113
1101	60-61	-0.14392083709819303
1101	62-63	-0.17272673299476793
1101	64-65	-0.2991804910863536
1101	66-67	-0.011785805296344876
1101	68-69	0.32448253906953184
1101	70-71	0.24041877343122664
1101	72-73	0.19902118631024734
1101	74-75	0.17839836969453415
1101	76-77	0.19799400542893153
1101	78-79	0.3038997276343558
1101	80-81	0.16270340301609565
1101	82-83	0.169155080021671
1101	84-85	0.262604879311386
1101	86-87	0.23374426220156153
1101	88-89	0.2373746505781682
1101	90-91	0.1747976546631982
1101	92-93	0.17320686711405386
1101	94-95	0.2569203706283396
1101	96-97	0.2567407026432953
1101	98-99	0.25644385136031644
1101	100-101	0.31286088491139097
1102	1	0.08355632341103103
1102	2	0.08813102165154874
1102	3	0.06349391022427398
1102	4	0.18235788791987773
1102	5	0.08711051729207497
1102	6	0.09461671202900845
1102	7	0.056239381228145646
1102	8	-0.007427255324934379
1102	9	0.13271162154096316
1102	10-11	0.12264734000985555
1102	12-13	0.11113679668991949
1102	14-15	0.14765850855931006
1102	16-17	0.2284937649794827
1102	18-19	0.2559482629963128
1102	20-21	0.24427627388931228
1102	22-23	0.29150923836368037
1102	24-25	0.296572791896601
1102	26-27	0.22881906343310732
1102	28-29	0.11455386716792759
1102	30-31	0.1652449074191651
1102	32-33	0.16021515370376704
1102	34-35	0.07499205002653753
1102	36-37	-0.01861048694600953
1102	38-39	0.012491700671539263
1102	40-41	0.005545949553095397
1102	42-43	0.11167294227065838
1102	44-45	0.10752412996871641
1102	46-47	0.0970377789571728
1102	48-49	0.08557424020277438
1102	50-51	-0.049301653696396386
1102	52-53	0.046071545571003014
1102	54-55	0.03119087838776835
1102	56-57	0.061562793207492916
1102	58-59	0.16038649877577882
1102	60-61	0.051471240747169134
1102	62-63	0.0296519890329634
1102	64-65	-0.009410768316065798
1102	66-67	0.23060520605379153
1102	68-69	0.4430933833974642
1102	70-71	0.39313796211104446
1102	72-73	0.38739516302000254
1102	74-75	0.22756570152944988
1102	76-77	0.24414705623556188
1102	78-79	0.36272467645122575
1102	80-81	0.2911162731655459
1102	82-83	0.3057029314767554
1102	84-85	0.39929809219782086
1102	86-87	0.30353177058690406
1102	88-89	0.32529960488485443
1102	90-91	0.2646918853085296
1102	92-93	0.3111291637784781
1102	94-95	0.4292969451180859
1102	96-97	0.45028299720146236
1102	98-99	0.506632032916098
1102	100-101	0.6860552947244472
1103	1	0.07491850208673156
1103	2	0.10679514702083281
1103	3	0.09999395341870354
1103	4	0.18719986408149225
1103	5	0.11334206998738239
1103	6	0.06176843469098259
1103	7	0.00446978393203068
1103	8	-0.019163242262969504
1103	9	0.11993648130962242
1103	10-11	0.08471203707312469
1103	12-13	0.08556192618585357
1103	14-15	0.04812896240106568
1103	16-17	0.13319213049393142
1103	18-19	0.1904420974452563
1103	20-21	0.22088986883083805
1103	22-23	0.2517260787936024
1103	24-25	0.20274811076473753
1103	26-27	0.18567065139757233
1103	28-29	0.18678035444698082
1103	30-31	0.22044729075294356
1103	32-33	0.2540883295863452
1103	34-35	0.2246923074518179
1103	36-37	0.12721519343698873
1103	38-39	0.09462287453045803
1103	40-41	0.13453732868516965
1103	42-43	0.10876188322649227
1103	44-45	0.11089584136254871
1103	46-47	0.11199983619021481
1103	48-49	0.08419922839786409
1103	50-51	-0.018886587862539272
1103	52-53	0.034528294997471676
1103	54-55	0.071572565219995
1103	56-57	0.14248038326761758
1103	58-59	0.2542156993372444
1103	60-61	0.1426357902305142
1103	62-63	0.09171535796580343
1103	64-65	0.15262002419692067
1103	66-67	0.23961635798917413
1103	68-69	0.3996915374530836
1103	70-71	0.2572872416913974
1103	72-73	0.2760558898340548
1103	74-75	0.1719137060931928
1103	76-77	0.22124445434051054
1103	78-79	0.38901519409622765
1103	80-81	0.2969731666218074
1103	82-83	0.25438261590393907
1103	84-85	0.41062570648712793
1103	86-87	0.31077798681893753
1103	88-89	0.31103720340833974
1103	90-91	0.2735704505221648
1103	92-93	0.3062073374840928
1103	94-95	0.394772340746929
1103	96-97	0.44953738536554866
1103	98-99	0.39787906027546427
1103	100-101	0.5106562873398381
1104	1	0.17019286657581034
1104	2	0.16437007073424326
1104	3	0.15124879879671482
1104	4	0.2692111940573909
1104	5	0.18693233403022447
1104	6	0.16082271533710468
1104	7	0.1576486186769941
1104	8	0.10685053790943044
1104	9	0.2887094087743165
1104	10-11	0.21609270339967424
1104	12-13	0.19372395116543117
1104	14-15	0.18506260645258266
1104	16-17	0.22996090299105987
1104	18-19	0.2362508816886475
1104	20-21	0.2471498187197696
1104	22-23	0.33388105272717183
1104	24-25	0.3039589669455367
1104	26-27	0.31521237649155154
1104	28-29	0.2503351962591438
1104	30-31	0.23375299481736533
1104	32-33	0.2883726132310116
1104	34-35	0.23109583216417917
1104	36-37	0.21455439978007718
1104	38-39	0.2223911322119818
1104	40-41	0.21064543996929075
1104	42-43	0.25311419719925254
1104	44-45	0.24662498716038783
1104	46-47	0.20763470706416598
1104	48-49	0.23816128760749677
1104	50-51	0.1333105378709334
1104	52-53	0.23376384709424514
1104	54-55	0.2729782696274867
1104	56-57	0.2757710581639756
1104	58-59	0.3802065647927151
1104	60-61	0.2683703255291263
1104	62-63	0.2682583437801327
1104	64-65	0.29441281514922224
1104	66-67	0.3642972449464068
1104	68-69	0.49398251341679256
1104	70-71	0.4132616300932561
1104	72-73	0.3814899893653134
1104	74-75	0.3243702473657848
1104	76-77	0.35592925222718463
1104	78-79	0.5173766159653077
1104	80-81	0.4376369165774143
1104	82-83	0.4148942330044285
1104	84-85	0.5733620850414844
1104	86-87	0.4537498124063468
1104	88-89	0.47606933651395167
1104	90-91	0.448978380478966
1104	92-93	0.4470066783269324
1104	94-95	0.5493076278780151
1104	96-97	0.5645802924973573
1104	98-99	0.540334973843585
1104	100-101	0.6614568035688784
1105	1	0.16288978474082683
1105	2	0.14339537816697856
1105	3	0.129252580238159
1105	4	0.23520625669486606
1105	5	0.14742915514485588
1105	6	0.1084864622391919
1105	7	0.1068558339532828
1105	8	0.1533882951760006
1105	9	0.28583727988127805
1105	10-11	0.23012376987464478
1105	12-13	0.15085126997291098
1105	14-15	0.12134963663692844
1105	16-17	0.20711834553404884
1105	18-19	0.1740974637970396
1105	20-21	0.21591426361647592
1105	22-23	0.26845678756292557
1105	24-25	0.2154888385567162
1105	26-27	0.26756537182480145
1105	28-29	0.1613674143764996
1105	30-31	0.10897696960979175
1105	32-33	0.20404791312235204
1105	34-35	0.17148470834339236
1105	36-37	0.16709703066613457
1105	38-39	0.1551131456299757
1105	40-41	0.13257470951315753
1105	42-43	0.16344446249180322
1105	44-45	0.1079010260149289
1105	46-47	0.13607868884940189
1105	48-49	0.16911779530749982
1105	50-51	0.009482594668909883
1105	52-53	0.04271427366588654
1105	54-55	0.11741801784734207
1105	56-57	0.13102040042915064
1105	58-59	0.30333639436537396
1105	60-61	0.22054182035213898
1105	62-63	0.20547726393080978
1105	64-65	0.21851279103081822
1105	66-67	0.2149184603658263
1105	68-69	0.2343955384452414
1105	70-71	0.1275158265549976
1105	72-73	0.22164845293169932
1105	74-75	0.19255685460374394
1105	76-77	0.15178467414947505
1105	78-79	0.32366144530982766
1105	80-81	0.22964987620891364
1105	82-83	0.23735182643122954
1105	84-85	0.42045798582414307
1105	86-87	0.2618554958696748
1105	88-89	0.26116778837997856
1105	90-91	0.18780523383326297
1105	92-93	0.21864486672640027
1105	94-95	0.29438791895478644
1105	96-97	0.2853104970624294
1105	98-99	0.2904236331874053
1105	100-101	0.34921960890883597
1106	1	0.06559436440804944
1106	2	0.060124289356465965
1106	3	0.05270947373647772
1106	4	0.17120112427926415
1106	5	0.0613870120699076
1106	6	0.025945737344976294
1106	7	0.03587861790101954
1106	8	0.042086653414422415
1106	9	0.2527058731102727
1106	10-11	0.13414677498443695
1106	12-13	0.05012978053432704
1106	14-15	0.0028056162040144272
1106	16-17	0.10983810979656994
1106	18-19	0.012278676544525524
1106	20-21	5.402360156736563E-4
1106	22-23	0.04231852101661815
1106	24-25	0.04425412127518058
1106	26-27	-0.01828826418589813
1106	28-29	-0.02663672734504985
1106	30-31	-0.09618009716760412
1106	32-33	1.4608102701885173E-4
1106	34-35	-0.04505099538813795
1106	36-37	-0.030739182701175594
1106	38-39	-0.008744722246795789
1106	40-41	-0.1318865640578224
1106	42-43	-0.1072050123798789
1106	44-45	-0.16002064857269716
1106	46-47	-0.08481452621206387
1106	48-49	-0.05413066134524058
1106	50-51	-0.2921169949438891
1106	52-53	-0.2900267498699023
1106	54-55	-0.17824172846241737
1106	56-57	-0.17957593731745902
1106	58-59	-0.11609588569903906
1106	60-61	-0.14663381300841394
1106	62-63	-0.21009414521195424
1106	64-65	-0.13967535360760408
1106	66-67	-0.10331524169785311
1106	68-69	-0.07329752231789044
1106	70-71	-0.2015085965115695
1106	72-73	-0.1227757883116567
1106	74-75	-0.16177393391011918
1106	76-77	-0.15595999016178297
1106	78-79	0.06059953689735309
1106	80-81	0.0023230791383745952
1106	82-83	-0.06886317607210302
1106	84-85	0.11071382889784687
1106	86-87	-6.321324263680594E-5
1106	88-89	-0.09461014469649243
1106	90-91	-0.07218136010635945
1106	92-93	-0.10205988746288241
1106	94-95	-0.037237847287777015
1106	96-97	-0.03462898319888197
1106	98-99	-0.05338976963673048
1106	100-101	0.05235341374848801
1107	1	-0.07647545072069661
1107	2	-0.07184564307286934
1107	3	-0.09483629142760464
1107	4	0.03682425950437107
1107	5	-0.06085818808879395
1107	6	-0.0825400145048647
1107	7	-0.02897692357728232
1107	8	-0.0030543056665521817
1107	9	0.11404911738056
1107	10-11	0.015954767847844664
1107	12-13	-0.05125077581109139
1107	14-15	-0.10067709001516079
1107	16-17	-0.09237344786587443
1107	18-19	-0.16045296570295164
1107	20-21	-0.15881234878300177
1107	22-23	-0.11079391277925765
1107	24-25	-0.1434348996955137
1107	26-27	-0.2105726059155728
1107	28-29	-0.24868129172573816
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2308	20-21	-0.7904128053227666
2308	22-23	-0.7008146556477257
2308	24-25	-0.6630430799174363
2308	26-27	-0.5995374902959867
2308	28-29	-0.6425985126987612
2308	30-31	-0.7713326056673395
2308	32-33	-0.7077563668409468
2308	34-35	-0.7057966638980702
2308	36-37	-0.6809200340896382
2308	38-39	-0.7182752051476129
2308	40-41	-0.665822454712405
2308	42-43	-0.7749374869689163
2308	44-45	-0.7580859511206981
2308	46-47	-0.751112508159089
2308	48-49	-0.6897827603755147
2308	50-51	-0.6102548260386484
2308	52-53	-0.7100873669487058
2308	54-55	-0.8059680013165433
2308	56-57	-0.7112562637798092
2308	58-59	-0.6248762271547577
2308	60-61	-0.6409992198730095
2308	62-63	-0.66565139669056
2308	64-65	-0.7198723106601896
2308	66-67	-0.13352152008857487
2308	68-69	-0.6911530435216449
2308	70-71	-0.7324870900841383
2308	72-73	-0.721057154981871
2308	74-75	-0.6975433596025624
2308	76-77	-0.7908080087588161
2308	78-79	-0.6661902798466279
2308	80-81	-0.860482980805255
2308	82-83	-0.8405383871567871
2308	84-85	-0.8022699334004351
2308	86-87	-1.0737606314484474
2308	88-89	-1.2475291110886886
2308	90-91	-1.2614532176530382
2308	92-93	-1.45960991249909
2308	94-95	-1.6154242413164006
2308	96-97	-1.9268356887649496
2308	98-99	-2.2012414211334494
2308	100-101	-2.5997214588835895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	419386.0
3	110015.0
4	79700.0
5	74269.0
6	98723.0
7	146451.0
8	205469.0
9	328938.0
10	451359.0
11	443567.0
12	373494.0
13	314503.0
14	272487.0
15	256390.0
16	254895.0
17	266271.0
18	290572.0
19	307959.0
20	341276.0
21	385710.0
22	442405.0
23	477563.0
24	617620.0
25	1127355.0
26	445704.0
27	433045.0
28	381123.0
29	362687.0
30	406354.0
31	463599.0
32	544993.0
33	675215.0
34	931011.0
35	1916689.0
36	2397917.0
37	4677961.0
38	8273828.0
39	3173488.0
40	6933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.512097906816535	20.406598355674006	8.230097725028358	41.85120601248109
2	17.991777666915702	20.348894979863726	48.216747857306025	13.44257949591455
3	18.07253494665774	24.504365538419524	38.12293241977563	19.300167095147106
4	18.59675718935658	28.026279549086492	34.30581174376927	19.07115151778766
5	23.74747341003614	28.260867138677913	31.287025114894774	16.70463433639117
6	22.608454639246926	32.512733751451215	31.81429157073785	13.064520038564012
7	15.9455702525026	22.188040327270816	49.612530230254784	12.253859189971797
8	11.567036674350174	29.3698599702239	42.764673422838726	16.2984299325872
9	19.10527329756651	23.5955610061296	42.19917682325113	15.09998887305276
10-11	19.76485549220551	34.28470611985765	33.627358562693956	12.323079825242884
12-13	19.797475337163686	24.979071349442794	37.587910749110726	17.635542564282797
14-15	17.94038177063626	26.365117544948653	40.42427167092605	15.27022901348903
16-17	21.441337426364573	25.9961810069426	36.77432055392407	15.788161012768755
18-19	20.116793464407298	26.806047147797845	38.92065936617757	14.156500021617285
20-21	19.54602695395081	30.192222131181463	36.38199997156466	13.879750943303062
22-23	24.65743706072712	26.895941410573077	34.88275854169613	13.563862987003676
24-25	18.92468673038045	33.550576471639985	33.604561787108395	13.920175010871173
26-27	19.42008423907731	33.330783069645435	33.3110170152445	13.938115676032753
28-29	20.684634520023884	32.88892872300196	32.268965026519716	14.157471730454438
30-31	21.79785764649968	28.59769864037316	35.71858936468449	13.885854348442672
32-33	18.532948414476312	31.0625573732839	36.20410722825935	14.200386983980435
34-35	20.64152555852303	32.91795861264365	32.414830173410095	14.025685655423226
36-37	15.78926335816904	31.50510343849437	36.71399522581682	15.99163797751977
38-39	16.448875210717297	28.560719905281157	36.66045833186107	18.329946552140473
40-41	21.660462380327957	28.15275008432156	33.779566179202895	16.407221356147588
42-43	19.75680804065984	28.462634221928578	37.11542029438643	14.665137443025145
44-45	23.194346596743216	27.624509622236005	34.41163578337025	14.769507997650535
46-47	17.971151989559004	29.29624472556302	34.03105319458861	18.701550090289366
48-49	17.581672461358096	28.467173622633375	33.208246823066794	20.74290709294174
50-51	20.507689513582665	27.827021022664816	34.91183308541872	16.7534563783338
52-53	16.554554185689618	32.74702066403897	36.49056849750436	14.207856652767056
54-55	16.549674000464364	31.59802693351675	33.33715580879175	18.515143257227137
56-57	16.40001387717178	30.867328661613396	37.61107954118164	15.121577920033188
58-59	16.16906347423212	35.40642594200701	33.22845472901672	15.196055854744147
60-61	15.189717063350184	37.63650868832359	32.09690402571964	15.076870222606587
62-63	15.60943897206825	38.70373341542274	31.057593713058747	14.62923389945026
64-65	15.68271004406608	37.55345722333273	32.1319705267622	14.631862205838992
66-67	16.022078132011185	38.85876351139608	30.714306318121753	14.404852038470983
68-69	17.38115198906999	37.43821908440424	30.940667413531436	14.239961512994338
70-71	15.694931959237257	38.47626904775214	30.63250443715929	15.196294555851313
72-73	16.817496723662483	36.72879587318994	31.21832614392025	15.235381259227331
74-75	17.337906311025097	36.080751488594906	31.124205486922175	15.457136713457825
76-77	17.569506313938664	34.996861070321366	31.85368144849156	15.579951167248407
78-79	17.24970938677879	35.74800104838231	31.518909349638406	15.483380215200498
80-81	18.138786766368092	35.49115493648537	31.29290708204293	15.077151215103607
82-83	18.00270422899965	33.975006242290576	31.913944161912056	16.108345366797717
84-85	17.854666092613048	33.90515498061243	31.857508851634346	16.38267007514018
86-87	19.52459185185462	34.19755701282012	30.76882896075598	15.509022174569287
88-89	19.120588324078835	33.69658422072314	31.732663615302748	15.450163839895282
90-91	18.80329365113932	33.68795042372689	31.501376705087132	16.007379220046662
92-93	19.82107557956627	33.39618286632482	30.722762094028948	16.05997946007997
94-95	19.693865900413858	33.27850496788937	29.953202933634536	17.074426198062227
96-97	19.45008740525358	33.90091350482566	31.060752532610795	15.588246557309967
98-99	19.542907983994265	33.834397831842004	30.53619790507706	16.086496279086667
100-101	20.04924714288595	34.37063917086265	29.229523316436058	16.35059036981534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	304877.0
1	238438.0
2	151121.5
3	145207.0
4	177240.5
5	221527.0
6	211323.0
7	166734.5
8	157015.5
9	152758.5
10	151565.0
11	152432.5
12	163916.5
13	170763.0
14	202336.0
15	218340.0
16	194390.0
17	193891.0
18	207022.5
19	229940.5
20	260280.0
21	298143.0
22	344279.0
23	392801.5
24	454804.5
25	546903.5
26	641957.5
27	732889.0
28	820948.5
29	909130.5
30	992262.5
31	1037854.0
32	1075421.0
33	1116255.5
34	1157784.0
35	1177393.5
36	1199365.5
37	1230605.5
38	1257637.5
39	1288424.0
40	1313946.5
41	1289214.5
42	1163482.0
43	1050890.0
44	984485.0
45	916030.5
46	839417.5
47	750671.0
48	662373.5
49	571489.5
50	471966.0
51	378512.0
52	309890.0
53	266640.5
54	275739.5
55	370770.0
56	325506.0
57	174113.0
58	118853.5
59	86940.0
60	63988.5
61	46746.5
62	34818.5
63	26507.5
64	19860.0
65	14760.0
66	11117.5
67	8567.5
68	6630.0
69	5189.0
70	4207.5
71	3538.0
72	3016.0
73	2540.5
74	2148.0
75	1831.0
76	1515.0
77	1219.0
78	956.0
79	767.0
80	621.5
81	489.0
82	365.5
83	268.5
84	203.5
85	160.0
86	119.0
87	81.0
88	52.5
89	39.0
90	31.0
91	25.5
92	20.5
93	13.5
94	11.5
95	9.5
96	7.5
97	6.0
98	3.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11892603425199998
2	0.14066403503832967
3	0.13714652991941026
4	0.07462114329827563
5	0.08736494076424926
6	0.14656874157471622
7	0.14550776316695302
8	0.1497546909412096
9	0.1511231119557678
10-11	0.1601188223477258
12-13	0.1725717549945257
14-15	0.19173718455635008
16-17	0.22449639996764015
18-19	0.23634198275885973
20-21	0.2540877508716601
22-23	0.26591826294686033
24-25	0.2521797982236087
26-27	0.24808508468114765
28-29	0.21994052251498664
30-31	0.22243472601619127
32-33	0.26433734483642907
34-35	0.2753012907405159
36-37	0.2877105183108597
38-39	0.2800289743557902
40-41	0.2825683900050529
42-43	0.28173196526597827
44-45	0.2898927579904635
46-47	0.28871422799774926
48-49	0.0442325515168314
50-51	0.024854624859134016
52-53	0.004631231032750353
54-55	0.001033851118928325
56-57	4.069093325228102E-4
58-59	4.641780533963908E-4
60-61	2.953860339795214E-4
62-63	2.7428703155241277E-4
64-65	1.4015765898007905E-4
66-67	6.932529368907136E-5
68-69	3.616971844647201E-5
70-71	3.1648503640663014E-5
72-73	4.6719219660026345E-5
74-75	0.0
76-77	3.0141432038726675E-5
78-79	2.8785067596983976E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	5.289821322796532E-4
90-91	7.444933713565489E-4
92-93	2.0044052305753237E-4
94-95	1.7482030582461472E-4
96-97	4.2951540655185517E-4
98-99	5.741942803377431E-4
100-101	5.335033470854622E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3176924E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.518761555051896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.35175455357485	24.251412089709152
2	14.300238631728202	9.586525777544782
3	5.005536209007176	5.033381239346698
4	2.3551094304288167	3.1576140573839035
5	1.2929488691266142	2.166902242356449
6	0.8445271513923153	1.6984502528571737
7	0.5661821530965563	1.3284407210458553
8	0.4219832146641931	1.1315483802050692
9	0.3251311202035419	0.9808193243006493
>10	2.162936174944154	13.734161577055263
>50	0.20749308341395717	4.8290371593708965
>100	0.14249270598300037	9.281228286080102
>500	0.013628328378830306	3.14211388599265
>1k	0.0089678702467156	5.8891961293291075
>5k	6.333814216401092E-4	1.4547564046845944
>10k+	4.371223895326642E-4	12.334412472737743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1077233	3.246934525937365	Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	825021	2.486731440202232	Illumina Single End PCR Primer 1 (100% over 50bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	275365	0.8299895433343971	Illumina Single End PCR Primer 1 (97% over 44bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	262179	0.790245051048132	No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	234292	0.7061896395217351	Illumina Single End PCR Primer 1 (100% over 43bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	130749	0.3940962097631474	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119132	0.35908090816375865	No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113948	0.3434555897948827	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	98413	0.29663087512272085	Illumina Single End PCR Primer 1 (98% over 50bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	68121	0.20532644919100998	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63559	0.19157592789494288	No Hit
GCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	57341	0.17283398545326262	Illumina Single End PCR Primer 1 (100% over 40bp)
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT	52681	0.158788078123216	No Hit
CGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	51207	0.1543452310407077	Illumina Single End PCR Primer 1 (100% over 41bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC	50848	0.1532631536305174	Illumina Single End PCR Primer 1 (100% over 47bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	43298	0.13050637244127875	No Hit
CCGAGATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	38550	0.11619522050929133	Illumina Single End PCR Primer 1 (97% over 42bp)
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	37813	0.11397379696803718	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	33513	0.10101298119138472	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7292637497074774	0.0	0.0	6.631115048519869E-5	0.0
2	3.746456422542367	9.042429611618003E-6	0.0	9.34384393200527E-5	0.0
3	3.7470592511831415	9.042429611618003E-6	0.0	1.1755158495103404E-4	6.0282864077453355E-6
4	4.910651150178961	9.042429611618003E-6	0.0	1.3563644417427005E-4	6.0282864077453355E-6
5	4.925616371186189	9.042429611618003E-6	0.0	1.5070716019363337E-4	6.0282864077453355E-6
6	4.930321448727435	9.042429611618003E-6	0.0	1.6276373300912406E-4	6.0282864077453355E-6
7	4.9964125667587505	9.042429611618003E-6	0.0	2.5921631553304944E-4	6.0282864077453355E-6
8	5.8198734759135595	1.2056572815490671E-5	0.0	2.622304587369221E-4	6.0282864077453355E-6
9	5.823987781386846	1.2056572815490671E-5	0.0	3.436123252414841E-4	6.0282864077453355E-6
10-11	6.122068459390629	1.2056572815490671E-5	0.0	3.6621839927052915E-4	6.0282864077453355E-6
12-13	6.2835346037504864	1.2056572815490671E-5	0.0	3.9786690291119213E-4	6.0282864077453355E-6
14-15	6.330208611262455	1.2056572815490671E-5	0.0	4.400649077654095E-4	6.0282864077453355E-6
16-17	6.371717884394587	1.959193082517234E-5	0.0	4.7623462621188147E-4	6.0282864077453355E-6
18-19	6.393501097328975	2.1099002427108674E-5	0.0	5.546023495125708E-4	6.0282864077453355E-6
20-21	6.405676728801018	2.4113145630981342E-5	0.0	6.555761468423052E-4	6.0282864077453355E-6
22-23	6.411231794725756	2.712728883485401E-5	0.0	6.947600084926499E-4	6.0282864077453355E-6
24-25	6.415814799467244	2.712728883485401E-5	0.0	7.670994453855939E-4	6.0282864077453355E-6
26-27	6.420673598311887	2.712728883485401E-5	0.0	0.001029329904122516	6.0282864077453355E-6
28-29	6.426291961243906	2.712728883485401E-5	0.0	0.0012177138543645579	6.0282864077453355E-6
30-31	6.432945682366454	3.3155575242599347E-5	0.0	0.0013126593652865468	6.0282864077453355E-6
32-33	6.439819435942886	3.3155575242599347E-5	0.0	0.0013443078689272098	6.0282864077453355E-6
34-35	6.447192030219559	3.3155575242599347E-5	0.0	0.0013744493009659364	6.0282864077453355E-6
36-37	6.455449275526568	3.466264684453568E-5	0.0	0.001452817024266626	6.0282864077453355E-6
38-39	6.465332651092066	3.616971844647201E-5	0.0	0.0014678877402859892	6.0282864077453355E-6
40-41	6.4767818740519765	3.616971844647201E-5	0.0	0.0015085786735382703	6.0282864077453355E-6
42-43	6.489894904060424	3.7676790048408344E-5	0.0	0.0015779039672273415	6.0282864077453355E-6
44-45	6.51822935724843	4.0690933252281015E-5	0.0	0.0016185949004796224	6.0282864077453355E-6
46-47	6.845256359510604	4.219800485421735E-5	0.0	0.0017060050533919298	6.0282864077453355E-6
48-49	6.890934192693693	4.521214805809001E-5	0.0	0.0017617667026635741	6.0282864077453355E-6
50-51	6.911945784967889	4.9733362863899016E-5	0.0	0.0017994434927119825	6.0282864077453355E-6
52-53	6.936196074114647	5.425457766970802E-5	0.0	0.001828077853148773	6.0282864077453355E-6
54-55	6.962670800945862	6.028286407745335E-5	0.0	0.001868768786401054	6.0282864077453355E-6
56-57	6.987002471959125	7.384650849488037E-5	0.0	0.0018853465740223535	6.0282864077453355E-6
58-59	7.025996442587625	9.79596541258617E-5	0.0	0.0019230233640707619	6.0282864077453355E-6
60-61	7.077518699443022	1.0549501213554338E-4	0.0	0.0019516577245075523	6.0282864077453355E-6
62-63	7.114244527310609	1.0850915533941604E-4	0.0	0.002040574949021796	6.0282864077453355E-6
64-65	7.157355817555599	1.1001622694135237E-4	0.0	0.0021264780303321667	6.0282864077453355E-6
66-67	7.201653173151314	1.115232985432887E-4	0.0	0.0021701831067883204	6.0282864077453355E-6
68-69	7.245240698022517	1.205657281549067E-4	0.0	0.0021822396796038115	6.0282864077453355E-6
70-71	7.302428037029593	1.2357987135877936E-4	0.0	0.002201831610428984	6.0282864077453355E-6
72-73	7.355460379630131	1.2357987135877936E-4	0.0	0.002230465970865774	6.0282864077453355E-6
74-75	7.4147440552355	1.2357987135877936E-4	0.0	0.002320890266981954	6.0282864077453355E-6
76-77	7.501586042153878	1.250869429607157E-4	0.0	0.0025303732196511044	6.0282864077453355E-6
78-79	7.575681217463078	1.2810108616458836E-4	0.0	0.0025936702269324305	6.0282864077453355E-6
80-81	7.6538469931691075	1.2960815776652472E-4	0.0	0.0026645025922234383	6.0282864077453355E-6
82-83	7.741722831206413	1.2960815776652472E-4	0.0	0.002712728883485401	6.0282864077453355E-6
84-85	7.844327280009443	1.3111522936846105E-4	0.0	0.002727799599504764	6.0282864077453355E-6
86-87	7.964829711157067	1.3412937257233372E-4	0.0	0.002748898601931873	6.0282864077453355E-6
88-89	8.103264787296133	1.3563644417427005E-4	0.0	0.0027699976043589814	6.0282864077453355E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	94080	0.0	63.314793	1
GAGCGGC	14240	0.0	53.04479	9
ACCACCG	53180	0.0	51.44225	1
GCGTGTT	115440	0.0	51.12285	2
CCACCGA	55320	0.0	49.358566	2
ATCGGGA	9550	0.0	48.324856	2
CACCGAG	57030	0.0	48.008457	3
ACCGAGA	57460	0.0	47.800053	4
CCGAGAT	108780	0.0	44.20721	1
GATCCGG	3700	0.0	43.537106	5
GCGACCA	20135	0.0	43.314358	1
CGAGATC	113315	0.0	42.296078	2
CGTGTTT	145260	0.0	41.95991	3
TCGGGAG	9880	0.0	41.75284	3
TACGGCG	5070	0.0	41.622646	2
CGGAGAT	47535	0.0	41.37348	42-43
TCTCGGA	49175	0.0	41.357693	40-41
TCGGAGA	49025	0.0	41.25153	42-43
ATACGGC	5460	0.0	40.564	1
CTCGGAG	49390	0.0	40.29139	40-41
>>END_MODULE