FastQCFastQC Report
Fri 27 May 2016
SRR1233053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33176924
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC10741183.237545469857302TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG9498222.862899526188745TruSeq Adapter, Index 7 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2182210.6577493440922973No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC2025830.6106141726701366TruSeq Adapter, Index 7 (100% over 42bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT895030.2697748591762154No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT876990.26433734483642907No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT830650.2503698052296831No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT644890.19437908107454446No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT539630.16265220971058075No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT473370.1426804968417205TruSeq Adapter, Index 7 (100% over 46bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA369530.11138163381270669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT382550.096.170971
CGGAGAT272550.089.033571
GTGGTCG144700.085.378313
GGTAGAT339850.077.69255
GGTGGTA308200.077.454362
GTCGCCG63750.076.391526
GTGGTAG313950.076.2826463
GGTGGTC170200.074.2563252
GGTCGCC69100.071.504355
GTAGATC370700.071.265156
CCGAGAT322400.070.7847751
CGCGTGT801050.068.681071
CGGCACT87950.067.748538
GAGATCT460900.066.882583
TGGTAGA361150.066.584824
TCGCCGA47900.066.297487
GAGCGGA90500.066.228652
AGCGGAA90700.066.025653
GGTCGAG74300.065.3534555
GCGGAAG92650.065.026394
Produced by FastQC (version 0.11.5)