##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233053_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33176924
Sequences flagged as poor quality	0
Sequence length	101
%GC	35
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.211624079435452	33.0	31.0	34.0	23.0	34.0
2	30.565458901494303	34.0	31.0	34.0	25.0	34.0
3	30.986932573978226	34.0	31.0	34.0	26.0	34.0
4	29.787450216903775	37.0	31.0	37.0	2.0	37.0
5	31.455818086088993	37.0	31.0	37.0	19.0	37.0
6	33.219390712653166	37.0	32.0	37.0	28.0	37.0
7	33.50463662634908	37.0	35.0	37.0	28.0	37.0
8	33.29528421622209	37.0	35.0	37.0	28.0	37.0
9	34.74990604915634	39.0	37.0	39.0	27.0	39.0
10-11	35.05848626895007	39.0	37.0	39.0	27.5	39.0
12-13	35.18721135811144	39.0	37.0	39.0	29.0	39.0
14-15	36.46952571612727	41.0	38.0	41.0	27.0	41.0
16-17	36.29007294347119	41.0	38.0	41.0	27.0	41.0
18-19	35.972891941398785	41.0	37.5	41.0	26.0	41.0
20-21	35.66056423735968	40.0	37.0	41.0	20.5	41.0
22-23	35.22488783167481	40.0	35.5	41.0	17.0	41.0
24-25	34.96618622630597	40.0	35.0	41.0	10.0	41.0
26-27	34.533525380472284	40.0	35.0	41.0	2.0	41.0
28-29	34.13848663305856	40.0	35.0	41.0	2.0	41.0
30-31	33.797757260438004	40.0	35.0	41.0	2.0	41.0
32-33	33.4585575504227	40.0	35.0	41.0	2.0	41.0
34-35	33.17160902258449	40.0	35.0	41.0	2.0	41.0
36-37	32.91573088572045	40.0	34.5	41.0	2.0	41.0
38-39	32.717134656606504	40.0	34.0	41.0	2.0	41.0
40-41	32.45932002315827	39.5	33.5	41.0	2.0	41.0
42-43	31.843416375791797	38.5	32.5	40.0	2.0	41.0
44-45	31.802904316867952	38.0	33.0	40.0	2.0	41.0
46-47	31.597988499476322	38.0	33.0	40.0	2.0	41.0
48-49	31.303997049274372	38.0	32.0	40.0	2.0	41.0
50-51	30.96613472966933	38.0	31.0	40.0	2.0	41.0
52-53	30.695970458261893	38.0	31.0	40.0	2.0	41.0
54-55	30.37121337710512	37.5	30.5	40.0	2.0	41.0
56-57	30.14579470959996	37.0	30.0	40.0	2.0	40.5
58-59	29.669973005936296	36.0	28.5	39.0	2.0	40.0
60-61	29.21041714114304	36.0	28.0	39.0	2.0	40.0
62-63	28.75832531973127	35.5	27.0	38.5	2.0	40.0
64-65	27.990027149593494	35.0	25.5	38.0	2.0	40.0
66-67	27.4170703257481	34.0	22.5	38.0	2.0	39.0
68-69	26.533960411760898	34.0	16.0	36.5	2.0	39.0
70-71	25.354792324930422	33.0	2.0	36.0	2.0	38.5
72-73	24.195372196048073	31.5	2.0	35.5	2.0	38.0
74-75	22.730797963668962	30.5	2.0	34.5	2.0	37.5
76-77	21.94160917389448	29.0	2.0	34.0	2.0	36.0
78-79	21.943299731463952	29.0	2.0	34.0	2.0	36.0
80-81	21.432048176015353	29.0	2.0	34.0	2.0	35.5
82-83	20.854049504408547	29.0	2.0	33.5	2.0	35.0
84-85	20.342213009259087	28.0	2.0	33.0	2.0	35.0
86-87	19.5115165739898	26.0	2.0	32.0	2.0	34.0
88-89	18.751756763224947	25.5	2.0	31.5	2.0	34.0
90-91	17.892009352645232	24.5	2.0	31.0	2.0	34.0
92-93	16.758463442843585	21.0	2.0	30.5	2.0	34.0
94-95	15.392225753056552	10.0	2.0	29.5	2.0	33.0
96-97	13.841618424299973	2.0	2.0	29.0	2.0	32.0
98-99	12.265732456089058	2.0	2.0	28.5	2.0	32.0
100-101	8.146466320988647	2.0	2.0	16.5	2.0	27.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2130312643282153
1101	2	0.39447854009022976
1101	3	0.3568487139782768
1101	4	4.605714180843378
1101	5	2.8450579070859128
1101	6	1.0198409302552136
1101	7	0.3383650975499748
1101	8	0.12581494261157644
1101	9	0.055602734180979496
1101	10-11	0.09260657927678295
1101	12-13	0.10228689045039374
1101	14-15	-0.08907285268689691
1101	16-17	-0.1037600316566838
1101	18-19	0.002396506137905874
1101	20-21	0.0997622165626666
1101	22-23	0.11072660720020622
1101	24-25	-0.09089662267947318
1101	26-27	-0.059754149734779105
1101	28-29	-0.07650180513228833
1101	30-31	0.0304841134637428
1101	32-33	-0.055910837771456556
1101	34-35	-0.13908609270367833
1101	36-37	-0.12624201510737265
1101	38-39	-0.16741321301644518
1101	40-41	-0.2089930548311969
1101	42-43	-0.10765831010902005
1101	44-45	-0.2963004427290663
1101	46-47	-0.34390609748802703
1101	48-49	-0.46881506522975513
1101	50-51	-0.4913057146019639
1101	52-53	-0.5432829853279557
1101	54-55	-0.612173652756919
1101	56-57	-0.650280262173883
1101	58-59	-0.7643110045416996
1101	60-61	-0.8538659487673073
1101	62-63	-0.9850359427314466
1101	64-65	-1.0221931807589328
1101	66-67	-0.9892223195510752
1101	68-69	-1.0525213912704139
1101	70-71	-1.2544644031385594
1101	72-73	-1.2105619811274586
1101	74-75	-0.7915953950678478
1101	76-77	-0.9413865090337765
1101	78-79	-0.9812758458859001
1101	80-81	-1.0297800169540423
1101	82-83	-1.3922674939273172
1101	84-85	-1.5593089046764135
1101	86-87	-1.7199419992866964
1101	88-89	-2.045883982015809
1101	90-91	-2.3927972050111013
1101	92-93	-2.8272693037752106
1101	94-95	-2.987905722090586
1101	96-97	-2.8810532077505506
1101	98-99	-2.655689453572288
1101	100-101	-1.1299030580144205
1102	1	-0.1877017652438191
1102	2	-0.12080297189439548
1102	3	-0.12946435939458567
1102	4	4.104792131560195
1102	5	2.368167042951182
1102	6	0.5593187241387412
1102	7	-0.04906882991532768
1102	8	-0.2892873692477025
1102	9	-0.3888113004818905
1102	10-11	-0.3152347017289472
1102	12-13	-0.2571665938968053
1102	14-15	-0.26650656611951007
1102	16-17	-0.2753545674773079
1102	18-19	-0.2926725291767198
1102	20-21	-0.28537282526364294
1102	22-23	-0.27421081023639715
1102	24-25	-0.33150260451488833
1102	26-27	-0.3704827730341762
1102	28-29	-0.4011845426860816
1102	30-31	-0.3308039001610297
1102	32-33	-0.4079698113653478
1102	34-35	-0.44033304371757964
1102	36-37	-0.44281453508428825
1102	38-39	-0.47655353398836553
1102	40-41	-0.4951775596648389
1102	42-43	-0.42229407459350554
1102	44-45	-0.5208401765553781
1102	46-47	-0.5399062800428673
1102	48-49	-0.6297663906946056
1102	50-51	-0.5993674842905143
1102	52-53	-0.6480453825471919
1102	54-55	-0.6352804047912777
1102	56-57	-0.6633026235633395
1102	58-59	-0.7383442166257197
1102	60-61	-0.8495043977840027
1102	62-63	-0.9639526053928371
1102	64-65	-0.9964160625947969
1102	66-67	-0.9209827775275272
1102	68-69	-0.9530237300739834
1102	70-71	-1.0212844799317757
1102	72-73	-1.0180255294423155
1102	74-75	-0.5715628114122353
1102	76-77	-0.7321862834675663
1102	78-79	-0.7918319983397559
1102	80-81	-0.8346628681771691
1102	82-83	-1.1455786789528624
1102	84-85	-1.2982032515034518
1102	86-87	-1.3520733206332984
1102	88-89	-1.569236799012618
1102	90-91	-1.9056292810957096
1102	92-93	-2.3024677106696885
1102	94-95	-2.410217867305665
1102	96-97	-2.403783780607693
1102	98-99	-2.2332319558090497
1102	100-101	-0.9768726874338496
1103	1	-0.1299186077075909
1103	2	-0.14065051977822307
1103	3	-0.12684901824522044
1103	4	4.12676685606057
1103	5	2.357541474130894
1103	6	0.5812466173127433
1103	7	0.00873852023643451
1103	8	-0.19038351546974042
1103	9	-0.27955046676234474
1103	10-11	-0.2530052400420786
1103	12-13	-0.18565410337961197
1103	14-15	-0.23884859382816614
1103	16-17	-0.26136281117901206
1103	18-19	-0.22281060916093054
1103	20-21	-0.22734728741441046
1103	22-23	-0.21177962793396432
1103	24-25	-0.27133026176319675
1103	26-27	-0.2727764297584159
1103	28-29	-0.2696005982425618
1103	30-31	-0.22150655665862473
1103	32-33	-0.3285571391794022
1103	34-35	-0.36305408504483694
1103	36-37	-0.3398781902911665
1103	38-39	-0.3419342111465866
1103	40-41	-0.36926362418106606
1103	42-43	-0.3058611139202547
1103	44-45	-0.37809037317407856
1103	46-47	-0.40024802255631187
1103	48-49	-0.44133304012415664
1103	50-51	-0.4887044818391466
1103	52-53	-0.48216096309799994
1103	54-55	-0.4895582705259649
1103	56-57	-0.5163633920727619
1103	58-59	-0.6467305440860436
1103	60-61	-0.6972895930772509
1103	62-63	-0.8135689948617753
1103	64-65	-0.8171279727314342
1103	66-67	-0.7763494647742988
1103	68-69	-0.8439318354055985
1103	70-71	-0.8226651007805224
1103	72-73	-0.9371908333115009
1103	74-75	-0.6144560059766953
1103	76-77	-0.676977932678593
1103	78-79	-0.7500534922488846
1103	80-81	-0.753957454672797
1103	82-83	-1.031849561309965
1103	84-85	-1.0769706656405127
1103	86-87	-1.1752428460410833
1103	88-89	-1.40021517277658
1103	90-91	-1.6814047940081345
1103	92-93	-2.1268055127235073
1103	94-95	-2.2358258902846995
1103	96-97	-2.136993182547366
1103	98-99	-1.9497154260303446
1103	100-101	-0.7476138919203219
1104	1	0.14153001499373374
1104	2	0.11600172081715954
1104	3	0.08801297027061494
1104	4	4.388390577051489
1104	5	2.652703189670955
1104	6	0.8334037861150208
1104	7	0.32178409892454596
1104	8	0.13160675351929996
1104	9	0.04748938946053016
1104	10-11	0.045321414365773194
1104	12-13	0.03976130005578682
1104	14-15	0.05824665272346863
1104	16-17	0.06671242946953981
1104	18-19	0.07280961562260302
1104	20-21	0.08602912685447706
1104	22-23	0.10174124575876675
1104	24-25	0.037591071247085495
1104	26-27	0.02661561903812526
1104	28-29	0.014556436730011058
1104	30-31	0.009398311785972169
1104	32-33	-0.02127380603671014
1104	34-35	-0.04521062127146536
1104	36-37	-0.04541890736943088
1104	38-39	-0.0447440752516286
1104	40-41	-0.048539546373355336
1104	42-43	-0.025741070525523213
1104	44-45	-0.09668841342480405
1104	46-47	-0.07776172975354356
1104	48-49	-0.42492941533126327
1104	50-51	-0.35840263468099565
1104	52-53	-0.21438385672925264
1104	54-55	-0.1842322765949227
1104	56-57	-0.1991575376731589
1104	58-59	-0.2739436907148125
1104	60-61	-0.2824928277894685
1104	62-63	-0.3597165907813782
1104	64-65	-0.4985808723516527
1104	66-67	-0.4249718001083025
1104	68-69	-0.4973018810151508
1104	70-71	-0.41190271734435413
1104	72-73	-0.46644358485472637
1104	74-75	-0.23412333260787577
1104	76-77	-0.3837598855369997
1104	78-79	-0.48011163692831005
1104	80-81	-0.5184881903017242
1104	82-83	-0.7065113786537047
1104	84-85	-0.8110371707121118
1104	86-87	-0.8382701727960473
1104	88-89	-0.9683042399596715
1104	90-91	-1.2344305562037654
1104	92-93	-1.7170879832017096
1104	94-95	-1.8660071842938404
1104	96-97	-1.7928501888839303
1104	98-99	-1.6628760168392276
1104	100-101	-0.5249647898160559
1105	1	0.2426968519859507
1105	2	0.20507718227906935
1105	3	0.1877644903022393
1105	4	4.462778140276782
1105	5	2.719810832435794
1105	6	0.8943550354568046
1105	7	0.45370544193577445
1105	8	0.28722502331380895
1105	9	0.09630032460058402
1105	10-11	0.059311640126246346
1105	12-13	0.11281029092940997
1105	14-15	0.19149148529566418
1105	16-17	0.18290445484400664
1105	18-19	0.13564570345965166
1105	20-21	0.1535605628824399
1105	22-23	0.14636000532192384
1105	24-25	0.11610728011044813
1105	26-27	0.08344651332274111
1105	28-29	0.07992311119517836
1105	30-31	0.07534774080333762
1105	32-33	0.03484537193288162
1105	34-35	0.02608519292671474
1105	36-37	-0.0047858288029161145
1105	38-39	-0.009729102361767161
1105	40-41	0.06387288242848399
1105	42-43	0.13068453402307867
1105	44-45	0.08985212958322464
1105	46-47	0.08898425094897888
1105	48-49	0.027102479127972146
1105	50-51	-0.01817178932840946
1105	52-53	-0.07412389526389873
1105	54-55	-0.05464156599101244
1105	56-57	-0.0899393144892251
1105	58-59	-0.19525411749081556
1105	60-61	-0.20083043369454145
1105	62-63	-0.24504569161208778
1105	64-65	-0.30510488644988953
1105	66-67	-0.3014768196542974
1105	68-69	-0.3436774073664388
1105	70-71	-0.2513784124392373
1105	72-73	-0.29579244551951334
1105	74-75	-0.04453528763218628
1105	76-77	-0.2518276459857951
1105	78-79	-0.3455959614784163
1105	80-81	-0.3411039396500435
1105	82-83	-0.47256004943385577
1105	84-85	-0.6003383022513127
1105	86-87	-0.6027540570398138
1105	88-89	-0.8111359597709367
1105	90-91	-1.0847605651071497
1105	92-93	-1.5033606720316612
1105	94-95	-1.5882764905357813
1105	96-97	-1.4626627839501651
1105	98-99	-1.3458242087400833
1105	100-101	-0.38059801066417265
1106	1	0.2438234711302094
1106	2	0.21444583961706343
1106	3	0.18067024961990796
1106	4	4.49711378847703
1106	5	2.6946045890139665
1106	6	0.8758619480870351
1106	7	0.44047158561854616
1106	8	0.32506393457832417
1106	9	0.19374550524408107
1106	10-11	0.09292195794676417
1106	12-13	0.09198355046516582
1106	14-15	0.13160770232025953
1106	16-17	0.07269662045509051
1106	18-19	0.07529201688963383
1106	20-21	0.07001878150545338
1106	22-23	0.15154603313145287
1106	24-25	0.06750732555225625
1106	26-27	-0.0020884715480988802
1106	28-29	0.03685305646008885
1106	30-31	0.03716899303302057
1106	32-33	0.056085682654938296
1106	34-35	-0.007904938542097284
1106	36-37	0.01062524764268602
1106	38-39	-0.03756692097371683
1106	40-41	0.03425351381650188
1106	42-43	0.06867468934379417
1106	44-45	0.0644751672458348
1106	46-47	0.018847032206931402
1106	48-49	0.042293441930979725
1106	50-51	-0.10905099727259326
1106	52-53	-0.15226776577693002
1106	54-55	-0.14298632808390366
1106	56-57	-0.11865290967396191
1106	58-59	-0.18496160182778
1106	60-61	-0.1652212979432477
1106	62-63	-0.23828525866970907
1106	64-65	-0.32861981539303997
1106	66-67	-0.24474426013063066
1106	68-69	-0.31915549815665756
1106	70-71	-0.19183449116532003
1106	72-73	-0.17904396428699343
1106	74-75	0.020874100702975795
1106	76-77	-0.2553777254193683
1106	78-79	-0.29179785719002993
1106	80-81	-0.2215593134570426
1106	82-83	-0.37220603615987713
1106	84-85	-0.44892510886485226
1106	86-87	-0.41818699913465807
1106	88-89	-0.5110953751326655
1106	90-91	-0.7280758367787072
1106	92-93	-1.1623567692771228
1106	94-95	-1.1799684312670369
1106	96-97	-1.0594788775047412
1106	98-99	-0.9953897008738224
1106	100-101	-0.3442084919766506
1107	1	0.5515384888285695
1107	2	0.4621988770612191
1107	3	0.42924526743751557
1107	4	4.787185531701873
1107	5	2.968869197068525
1107	6	1.097662957206829
1107	7	0.7132944131350811
1107	8	0.6909959541182857
1107	9	0.46487380885180585
1107	10-11	0.3160703037535839
1107	12-13	0.24011854363168794
1107	14-15	0.2927505880299819
1107	16-17	0.2441277878369803
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2308	22-23	-0.09689448352905572
2308	24-25	0.3053286555371386
2308	26-27	0.2931358372595767
2308	28-29	0.34764506032886544
2308	30-31	0.3768141142717738
2308	32-33	0.5731657253667706
2308	34-35	0.5386953641580874
2308	36-37	0.5571022377437131
2308	38-39	0.45975221523973175
2308	40-41	0.5876121835629036
2308	42-43	0.43371630245905735
2308	44-45	0.39585741250520456
2308	46-47	0.5062205087816132
2308	48-49	0.5870193697935946
2308	50-51	0.6693555805670748
2308	52-53	0.5073722036821202
2308	54-55	0.5884068371929025
2308	56-57	0.6460094993643146
2308	58-59	0.6561331915842707
2308	60-61	0.59647872923113
2308	62-63	0.6153120147550091
2308	64-65	0.6936174257399443
2308	66-67	0.46859194322023967
2308	68-69	0.526711398133596
2308	70-71	0.5160547178872257
2308	72-73	0.5880506146818014
2308	74-75	0.6365655987410577
2308	76-77	0.6121441970449375
2308	78-79	0.6669124941174438
2308	80-81	0.8497549028881899
2308	82-83	0.8597122073329864
2308	84-85	0.7155788594089252
2308	86-87	0.6705870143871273
2308	88-89	0.7086539536161176
2308	90-91	0.42452187231384997
2308	92-93	0.49398755587162313
2308	94-95	0.9259865322891745
2308	96-97	1.1520577716558318
2308	98-99	0.9125840528047426
2308	100-101	0.3311074551409394
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1426665.0
3	580495.0
4	252151.0
5	184342.0
6	262154.0
7	275072.0
8	218420.0
9	297405.0
10	445582.0
11	515211.0
12	432262.0
13	351720.0
14	301009.0
15	280811.0
16	267233.0
17	258966.0
18	255660.0
19	257693.0
20	266967.0
21	287404.0
22	321241.0
23	368015.0
24	442449.0
25	580425.0
26	830709.0
27	1069333.0
28	805959.0
29	613257.0
30	760964.0
31	1069211.0
32	1657170.0
33	2584164.0
34	3911034.0
35	5183318.0
36	4275134.0
37	1227836.0
38	59348.0
39	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.189352608907406	19.408287316872443	8.171495556154273	43.23086451806588
2	20.338859624237617	20.37703676205787	47.761679171944934	11.52242444175958
3	19.482517426871762	24.09540136994014	37.96915289675438	18.452928306433712
4	21.81237125703221	26.831708637130568	33.22442170905604	18.131498396781183
5	25.71432179788578	28.15750489707846	30.88569332105653	15.242479983979226
6	24.57536147715201	32.23216534480412	31.41747860651578	11.77499457152809
7	15.231638110874895	22.46561194160134	50.252952925955405	12.049797021568365
8	11.845401942627351	29.619837571439717	42.06745025548481	16.467310230448128
9	19.20485455493101	23.193672204210372	42.8586809313606	14.742792309498013
10-11	19.650463979119944	33.88112321684795	33.209544983736286	13.258867820295816
12-13	17.217703787126258	27.2101687305309	37.15004591745757	18.42208156488528
14-15	15.399991572455601	30.100040317179495	36.55027964617817	17.949688464186732
16-17	18.72227515727498	28.51807177784173	34.682598965473716	18.07705409940958
18-19	18.084833603018772	27.267888065813455	38.214300698883356	16.432977632284416
20-21	18.71503066408447	26.79733962075568	37.04106052749194	17.446569187667908
22-23	17.38082462426271	34.57461552168943	33.54645147118007	14.498108382867791
24-25	16.331029315890515	29.61373777351651	34.42169687651239	19.633536034080574
26-27	15.654499796304203	28.09247174331171	34.00449360525406	22.24853485513003
28-29	18.79168484697376	31.77325149251329	32.17862511907373	17.256438541439227
30-31	17.986580069930532	28.87593195800792	35.32725185734518	17.81023611471636
32-33	15.717406537240839	33.018522591798956	31.276932222530924	19.98713864842929
34-35	15.35940971470622	30.996761256206934	31.4471739551727	22.19665507391414
36-37	20.05926319729436	33.950026214011345	30.44675734458003	15.543953244114267
38-39	15.430883526152092	31.22609709085749	35.10659095460447	18.23642842838595
40-41	15.718647331194868	33.34007547084733	33.59180320449611	17.34947399346169
42-43	15.274668019253381	29.27417261467639	35.38364647668964	20.06751288938058
44-45	18.210218946156672	29.33921330380116	32.958209145609764	19.492358604432404
46-47	17.2816799137191	31.792634972112925	35.268721230889646	15.656963883278324
48-49	18.06380844710016	31.089393639989048	35.48968102045868	15.357116892452115
50-51	17.028364353488588	29.638058067107124	31.63703181162907	21.696545767775216
52-53	20.974687104808147	28.768052758598117	33.10631057900365	17.15094955759009
54-55	15.420250311147129	29.988864065171267	34.31726302590978	20.273622597771816
56-57	17.165568049164147	29.218178349701297	36.77718279188552	16.839070809249034
58-59	17.723193866816775	29.989319182789004	34.05096400512907	18.23652294526515
60-61	17.837477890200056	29.337791475791892	32.51926518716158	20.30546544684647
62-63	18.56107802989059	30.141611281736758	36.08386382279025	15.213446865582403
64-65	17.34322218669191	36.1595724418606	30.01748125437864	16.47972411706885
66-67	16.526171110570605	38.417898049596985	30.070985221536045	14.984945618296367
68-69	15.888333634045859	39.47415537105826	29.351748293187658	15.285762701708224
70-71	15.604329442033915	39.98424501102691	28.646890744486527	15.764534802452648
72-73	15.578187974163729	40.009633080285255	28.83975261379327	15.572426331757752
74-75	16.669315771611323	37.82188169928271	28.64491997176043	16.863882557345537
76-77	15.93747118640124	38.01764821715203	29.291313346701585	16.753567249745142
78-79	16.248083551098315	37.44171678855809	29.233030691426336	17.07716896891726
80-81	16.571966686984332	37.12224735065901	29.375658042743304	16.93012791961336
82-83	17.066680549837475	36.167613904678866	29.63776560494877	17.12793994053489
84-85	16.941631443102548	35.48257368128089	29.941497651440823	17.63429722417574
86-87	17.026212849694325	36.170954831668304	29.204219491595516	17.598612827041855
88-89	17.402033482733593	35.305975555603965	29.604313810947325	17.687677150715114
90-91	17.054890848110716	35.49732251033511	29.116734473573846	18.33105216798033
92-93	17.59641923780188	35.27748843578687	29.205635012541492	17.920457313869758
94-95	18.04855914169874	34.948675915739884	28.517599430069364	18.485165512492014
96-97	16.981137194534526	35.547964192040624	28.581619288874222	18.889279324550625
98-99	17.983885907909986	35.27676262983157	28.219327966390615	18.52002349586782
100-101	18.573099949335447	34.94584213444335	26.504268806831355	19.976789109389838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169246.0
1	178174.5
2	155618.5
3	120687.0
4	132005.5
5	168297.0
6	174288.0
7	153751.5
8	148499.5
9	147611.5
10	148461.0
11	151139.0
12	160552.0
13	169364.5
14	187069.0
15	199024.0
16	190523.0
17	189062.0
18	198163.0
19	215754.0
20	240481.0
21	271703.0
22	311134.5
23	355535.5
24	412792.0
25	497515.5
26	589187.5
27	676743.5
28	765022.5
29	856063.0
30	935603.0
31	984537.0
32	1027117.0
33	1067769.0
34	1108631.0
35	1140906.5
36	1180464.5
37	1214983.5
38	1232495.0
39	1255268.0
40	1278931.5
41	1262823.0
42	1181029.0
43	1136626.0
44	1152253.5
45	1159257.5
46	1120267.5
47	1025827.5
48	892468.0
49	739645.0
50	588234.0
51	460385.0
52	363643.5
53	288725.0
54	230116.5
55	188103.0
56	156961.5
57	129448.5
58	104678.5
59	82298.0
60	62177.0
61	46269.0
62	35180.5
63	26631.0
64	19412.5
65	13848.5
66	9915.0
67	7203.0
68	5097.5
69	3638.5
70	2537.5
71	1788.5
72	1333.5
73	958.5
74	667.5
75	482.0
76	353.5
77	240.0
78	171.5
79	126.0
80	96.5
81	80.5
82	62.0
83	47.5
84	37.5
85	32.5
86	34.5
87	31.5
88	27.0
89	21.0
90	17.5
91	18.0
92	14.0
93	9.0
94	7.5
95	6.5
96	4.0
97	1.0
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.2418940345403932
2	0.0
3	0.0
4	8.404549499525634
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.028286407745335E-5
24-25	5.877579247551702E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.9591930825172338E-5
34-35	5.274750606777169E-5
36-37	3.0141432038726675E-5
38-39	0.0
40-41	2.2606074029045006E-5
42-43	0.0
44-45	0.0
46-47	5.199397026680352E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.8785067596983976E-4
56-57	6.842105072790956E-4
58-59	4.686992682021998E-4
60-61	5.319962754835258E-4
62-63	9.946672572779805E-4
64-65	7.324367985410582E-4
66-67	0.0011167400570348232
68-69	5.003477718428629E-4
70-71	3.9635983130925574E-4
72-73	0.001868768786401054
74-75	0.006622072618908252
76-77	9.494551092198903E-4
78-79	0.0049220958519240665
80-81	0.00410677011527651
82-83	0.014669834973248273
84-85	0.017928123776634625
86-87	0.018538487775418843
88-89	0.03142696411517837
90-91	0.01702237374387089
92-93	0.013363203894369471
94-95	0.015577092077613946
96-97	0.013800254658931008
98-99	0.012908068270584699
100-101	0.008564687913804185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3176924E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.29693532027546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60817057251505	31.27371429885544
2	11.737179604306174	9.459447347143644
3	4.04127078672806	4.885524825134982
4	1.8556340833834415	2.9910546654480474
5	1.044864343251826	2.1052415429240465
6	0.6766645647356151	1.6360504919204057
7	0.4677340708497807	1.3193774720085925
8	0.3499721314639476	1.128224347640131
9	0.25839043684939933	0.9371108448988167
>10	1.6823037408349364	12.834303072347392
>50	0.15822047365046976	4.4357355143877655
>100	0.10342449950070018	7.9657269769177725
>500	0.009418965283926486	2.5991819464103125
>1k	0.00603540820860232	4.672466618478762
>5k	3.928197886576938E-4	1.0401924323780483
>10k+	3.234986493747632E-4	10.716647603105708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	1074118	3.237545469857302	TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG	949822	2.862899526188745	TruSeq Adapter, Index 7 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	218221	0.6577493440922973	No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC	202583	0.6106141726701366	TruSeq Adapter, Index 7 (100% over 42bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89503	0.2697748591762154	No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87699	0.26433734483642907	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	83065	0.2503698052296831	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	64489	0.19437908107454446	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53963	0.16265220971058075	No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT	47337	0.1426804968417205	TruSeq Adapter, Index 7 (100% over 46bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	36953	0.11138163381270669	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1784230509133398	0.0	0.0	6.329700728132601E-5	0.0
2	3.192833669571055	6.0282864077453355E-6	0.0	9.042429611618002E-5	0.0
3	3.1930295888793068	6.0282864077453355E-6	0.0	9.946672572779803E-5	0.0
4	3.3568000457185243	6.0282864077453355E-6	0.0	1.115232985432887E-4	0.0
5	3.3712558765242973	6.0282864077453355E-6	0.0	1.3563644417427005E-4	0.0
6	3.374987385810692	6.0282864077453355E-6	0.0	1.7180616262074206E-4	0.0
7	3.4274786897061342	6.0282864077453355E-6	0.0	2.3510316990206807E-4	0.0
8	4.260193018496833	9.042429611618003E-6	0.0	2.411314563098134E-4	0.0
9	4.264141546093906	9.042429611618003E-6	0.0	2.9237189077564876E-4	0.0
10-11	4.471707503685392	1.205657281549067E-5	0.0	3.089496783969484E-4	1.5070716019363339E-6
12-13	4.596716078922808	1.5070716019363337E-5	0.0	3.4059818203761145E-4	3.0141432038726678E-6
14-15	4.65091941615805	1.657778762129967E-5	0.0	3.8430325849376514E-4	3.0141432038726678E-6
16-17	4.7034197624831044	2.4113145630981342E-5	0.0	4.2499419174604617E-4	3.0141432038726678E-6
18-19	4.714340003310735	2.712728883485401E-5	0.0	5.289821322796532E-4	3.0141432038726678E-6
20-21	4.722820295214831	2.712728883485401E-5	0.0	6.299559296093875E-4	4.521214805809001E-6
22-23	4.728337684349519	3.0141432038726675E-5	0.0	6.76675149269414E-4	6.0282864077453355E-6
24-25	4.7331753841917354	3.0141432038726675E-5	0.0	7.490145861623579E-4	6.0282864077453355E-6
26-27	4.738290385208707	3.0141432038726675E-5	0.0	0.0010172733313070254	6.0282864077453355E-6
28-29	4.7438725784222795	3.0141432038726675E-5	0.0	0.00119209363713164	6.0282864077453355E-6
30-31	4.750579047050896	3.0141432038726675E-5	0.0	0.0012779967184420112	6.0282864077453355E-6
32-33	4.757502533990191	3.918386165034468E-5	0.0	0.0013066310788788014	6.0282864077453355E-6
34-35	4.7649489747753595	4.521214805809001E-5	0.0	0.001341293725723337	6.0282864077453355E-6
36-37	4.77277971881902	4.9733362863899016E-5	0.0	0.0014498028810627532	6.0282864077453355E-6
38-39	4.781281109725543	5.124043446583535E-5	0.0	0.00146336652548018	6.0282864077453355E-6
40-41	4.791123794357789	5.124043446583535E-5	0.0	0.001490493814315034	6.0282864077453355E-6
42-43	4.802613708250952	5.124043446583535E-5	0.0	0.0015522837499944238	6.0282864077453355E-6
44-45	4.815125416690227	5.124043446583535E-5	0.0	0.00161558075727575	6.0282864077453355E-6
46-47	4.829695784937748	5.124043446583535E-5	0.0	0.001760259631061638	6.0282864077453355E-6
48-49	4.844876517184053	5.7268720873580685E-5	0.0	0.001814514208731346	6.0282864077453355E-6
50-51	4.863240184653646	6.480407888326235E-5	0.0	0.0018356132111584547	6.0282864077453355E-6
52-53	4.882268470699694	7.384650849488036E-5	3.0141432038726678E-6	0.0018521909987797543	6.0282864077453355E-6
54-55	4.900345794564921	8.43960097084347E-5	3.0141432038726678E-6	0.0019049385048475259	6.0282864077453355E-6
56-57	4.919428335188639	8.741015291230736E-5	3.0141432038726678E-6	0.0019335728652843161	6.0282864077453355E-6
58-59	4.942230328525936	1.115232985432887E-4	6.0282864077453355E-6	0.0019697425837307886	6.0282864077453355E-6
60-61	4.965971227471238	1.2207279975684303E-4	6.0282864077453355E-6	0.001990841586157897	6.0282864077453355E-6
62-63	4.995640946098559	1.2659401456265203E-4	6.0282864077453355E-6	0.0020526315218372866	6.0282864077453355E-6
64-65	5.029094921518341	1.296081577665247E-4	6.0282864077453355E-6	0.002140041674749594	6.0282864077453355E-6
66-67	5.063884162377441	1.3412937257233372E-4	6.0282864077453355E-6	0.002201831610428984	6.0282864077453355E-6
68-69	5.0967202384404295	1.3865058737814272E-4	6.0282864077453355E-6	0.0022229306128560924	6.0282864077453355E-6
70-71	5.135111380428155	1.4166473058201538E-4	6.0282864077453355E-6	0.0022515649732928827	6.0282864077453355E-6
72-73	5.177787126980187	1.4166473058201538E-4	6.0282864077453355E-6	0.002266635689312246	6.0282864077453355E-6
74-75	5.222770200154782	1.4166473058201538E-4	6.0282864077453355E-6	0.0023465104842148718	6.0282864077453355E-6
76-77	5.284608362125434	1.4166473058201538E-4	6.0282864077453355E-6	0.0025755853677091947	6.0282864077453355E-6
78-79	5.347781789535401	1.4166473058201538E-4	6.0282864077453355E-6	0.0026524460194079476	6.0282864077453355E-6
80-81	5.417021180143163	1.4166473058201538E-4	6.0282864077453355E-6	0.00271725009829121	6.0282864077453355E-6
82-83	5.494903626388028	1.4166473058201538E-4	6.0282864077453355E-6	0.0027669834611551087	6.0282864077453355E-6
84-85	5.585383684153479	1.4166473058201538E-4	6.0282864077453355E-6	0.0027835612487764087	6.0282864077453355E-6
86-87	5.690340671727132	1.4166473058201538E-4	6.0282864077453355E-6	0.0028106885376112624	6.0282864077453355E-6
88-89	5.811671992255822	1.4166473058201538E-4	6.0282864077453355E-6	0.0028393228980480526	6.0282864077453355E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	38255	0.0	96.17097	1
CGGAGAT	27255	0.0	89.03357	1
GTGGTCG	14470	0.0	85.37831	3
GGTAGAT	33985	0.0	77.6925	5
GGTGGTA	30820	0.0	77.45436	2
GTCGCCG	6375	0.0	76.39152	6
GTGGTAG	31395	0.0	76.282646	3
GGTGGTC	17020	0.0	74.256325	2
GGTCGCC	6910	0.0	71.50435	5
GTAGATC	37070	0.0	71.26515	6
CCGAGAT	32240	0.0	70.784775	1
CGCGTGT	80105	0.0	68.68107	1
CGGCACT	8795	0.0	67.74853	8
GAGATCT	46090	0.0	66.88258	3
TGGTAGA	36115	0.0	66.58482	4
TCGCCGA	4790	0.0	66.29748	7
GAGCGGA	9050	0.0	66.22865	2
AGCGGAA	9070	0.0	66.02565	3
GGTCGAG	7430	0.0	65.353455	5
GCGGAAG	9265	0.0	65.02639	4
>>END_MODULE