FastQCFastQC Report
Fri 27 May 2016
SRR1233052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34937512
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11520913.297575969347789Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC9944812.8464562674067917Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940141.1277677700690305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3739091.0702221726607206No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1276880.3654753664199099Illumina Single End PCR Primer 1 (98% over 50bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC1090820.3122202863214759Illumina Single End PCR Primer 1 (97% over 44bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1088960.3116879072556741Illumina Single End PCR Primer 1 (100% over 43bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930080.2662124309252474No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT793520.22712550338444248No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT519270.148628213709093No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT508560.14556274070116956No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT506840.14507043317795498No Hit
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440150.12598206764122183No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402930.11532876181910148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC159050.066.566639
ACCACCG212400.059.3676761
TCGGGAG104150.058.2103233
ATCGGGA111550.058.184072
CCACCGA220000.057.0803642
CACCGAG227850.055.0522233
ACCGAGA236850.053.185724
AGAGCGG210050.050.012538
CGCGTGT983500.046.1458441
GATCGGG157400.043.5301671
CCGAGAT436850.042.8730771
GTATCAT3243250.040.88999650-51
CGCCGTA3276700.040.6763946-47
CCGTATC3281150.040.67156248-49
GAGCGTC3974050.040.589229
GTCGCCG3313000.040.38645644-45
GGCGCCG167900.040.08478544-45
ATACGGC16900.039.951461
ATCATTA3428900.039.88075352-53
AGAGCGT4045050.039.8760538
Produced by FastQC (version 0.11.5)