##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233052_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34937512
Sequences flagged as poor quality	0
Sequence length	101
%GC	29
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10508573134801	34.0	31.0	34.0	30.0	34.0
2	32.284972152567704	34.0	33.0	34.0	31.0	34.0
3	32.3662639457555	34.0	33.0	34.0	31.0	34.0
4	35.634718522601155	37.0	37.0	37.0	35.0	37.0
5	35.590194230201625	37.0	37.0	37.0	35.0	37.0
6	35.47678134607868	37.0	37.0	37.0	35.0	37.0
7	35.386591065786256	37.0	37.0	37.0	35.0	37.0
8	35.33222975350964	37.0	36.0	37.0	33.0	37.0
9	36.88475284101512	39.0	39.0	39.0	33.0	39.0
10-11	37.01353792737159	39.0	39.0	39.0	34.5	39.0
12-13	37.08201036181397	39.0	39.0	39.0	35.0	39.0
14-15	38.41968693992864	41.0	39.0	41.0	34.0	41.0
16-17	38.123695642666256	41.0	39.0	41.0	33.5	41.0
18-19	37.52350583808028	41.0	37.0	41.0	32.5	41.0
20-21	37.15198633777929	40.0	37.0	41.0	32.0	41.0
22-23	37.013933934391204	40.0	36.5	41.0	32.5	41.0
24-25	36.29193104820973	40.0	35.0	41.0	29.0	41.0
26-27	35.96879839354331	40.0	35.0	41.0	26.5	41.0
28-29	35.25930399680435	40.0	35.0	41.0	18.5	41.0
30-31	34.384694150516495	40.0	35.0	41.0	9.5	41.0
32-33	34.17487878072142	40.0	35.0	41.0	7.5	41.0
34-35	34.07142285918928	40.0	35.0	41.0	6.0	41.0
36-37	33.842571674823326	40.0	35.0	41.0	2.0	41.0
38-39	33.73701845168597	40.0	35.0	41.0	2.0	41.0
40-41	33.5802835931763	40.0	35.0	41.0	2.0	41.0
42-43	33.34583429982078	40.0	35.0	41.0	2.0	41.0
44-45	33.23585261308819	40.0	35.0	41.0	2.0	41.0
46-47	32.90679984596499	39.0	34.0	41.0	2.0	41.0
48-49	32.8856364900855	39.0	34.5	41.0	2.0	41.0
50-51	32.11515980302204	38.5	33.0	40.5	2.0	41.0
52-53	32.06481061101317	38.0	33.0	40.5	2.0	41.0
54-55	32.15288894927607	38.0	33.0	41.0	2.0	41.0
56-57	32.07270386053821	38.0	33.5	41.0	2.0	41.0
58-59	31.706176916661953	37.0	33.0	41.0	2.0	41.0
60-61	31.563343377170074	37.0	33.0	41.0	2.0	41.0
62-63	31.270942232520735	36.0	33.0	40.5	2.0	41.0
64-65	30.65876297945887	36.0	31.5	40.0	2.0	41.0
66-67	28.921587375769633	35.0	22.0	40.0	2.0	41.0
68-69	28.125187692243227	35.0	7.0	40.0	2.0	41.0
70-71	27.660419708764607	35.0	2.0	39.5	2.0	41.0
72-73	27.26511562987084	35.0	2.0	39.0	2.0	41.0
74-75	26.83610366988926	35.0	2.0	39.0	2.0	41.0
76-77	26.301467374093498	35.0	2.0	37.5	2.0	40.0
78-79	25.872663256616555	35.0	2.0	37.0	2.0	39.5
80-81	25.492595337069226	35.0	2.0	36.5	2.0	39.0
82-83	25.069674781077715	35.0	2.0	36.0	2.0	39.0
84-85	24.628418145516484	34.5	2.0	36.0	2.0	37.5
86-87	24.18336299963203	34.0	2.0	35.0	2.0	37.0
88-89	23.671508935725015	34.0	2.0	35.0	2.0	37.0
90-91	23.14318304921083	33.5	2.0	35.0	2.0	36.0
92-93	22.644615764282243	33.0	2.0	35.0	2.0	36.0
94-95	22.16260989048104	33.0	2.0	35.0	2.0	35.5
96-97	21.60109889908589	32.0	2.0	35.0	2.0	35.0
98-99	21.01767242326815	31.0	2.0	35.0	2.0	35.0
100-101	19.630657844210546	28.5	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.054069682857374346
1101	2	0.03660236995932564
1101	3	0.008046537136152665
1101	4	-0.05472367366074593
1101	5	-0.056335648923528936
1101	6	0.03466750177179989
1101	7	-0.11858768966632027
1101	8	-0.1473657010892424
1101	9	-0.06973771041688082
1101	10-11	-0.1148851880515096
1101	12-13	-0.10539004910859973
1101	14-15	-0.02343814398377475
1101	16-17	0.007908956199777606
1101	18-19	0.06522985038995444
1101	20-21	0.021203236315464835
1101	22-23	-0.07902726979627062
1101	24-25	-0.06049928631599499
1101	26-27	-0.10289144761151903
1101	28-29	-0.08360831075790287
1101	30-31	0.07473739141203595
1101	32-33	-0.003948088409380546
1101	34-35	-0.058529960114164226
1101	36-37	-0.09149057713896269
1101	38-39	-0.11940446340754107
1101	40-41	-0.11038235544402397
1101	42-43	0.03732267674925538
1101	44-45	0.03585328112328767
1101	46-47	-0.01863691881343499
1101	48-49	-0.08299386307390932
1101	50-51	-0.10436561579100356
1101	52-53	-0.1307770444992613
1101	54-55	-0.10079554709016492
1101	56-57	-0.12048993115537598
1101	58-59	-0.056146044822682484
1101	60-61	-0.09636821624846803
1101	62-63	-0.13726072948459844
1101	64-65	-0.22633962236178107
1101	66-67	-0.06150587152793818
1101	68-69	0.2566316567921447
1101	70-71	0.21980061921009053
1101	72-73	0.1929076638472793
1101	74-75	0.22100691134253836
1101	76-77	0.36574355964334515
1101	78-79	0.2739990837989481
1101	80-81	0.27854813257318156
1101	82-83	0.3032072650583899
1101	84-85	0.28172948837022815
1101	86-87	0.2402012439082064
1101	88-89	0.22862929415985533
1101	90-91	0.31062322727807157
1101	92-93	0.3044974268436391
1101	94-95	0.4112090744484007
1101	96-97	0.43898780947368365
1101	98-99	0.4367810979014166
1101	100-101	0.5499684252553578
1102	1	0.019437058177594224
1102	2	0.12273181252544418
1102	3	0.12266312756467812
1102	4	0.05578507368873176
1102	5	0.054460277753292985
1102	6	0.17552832486666148
1102	7	0.010160584957212393
1102	8	-0.0821415604627731
1102	9	-0.041405992382237855
1102	10-11	-0.022914532314452174
1102	12-13	0.005908890978410852
1102	14-15	0.09140099650911537
1102	16-17	0.09911899672818691
1102	18-19	0.1490016606326776
1102	20-21	0.1700419082975344
1102	22-23	0.16654388299762246
1102	24-25	0.14291072917899328
1102	26-27	0.0742160236767333
1102	28-29	0.017657936227948312
1102	30-31	0.12238923938298996
1102	32-33	0.11212242049133891
1102	34-35	0.08908715275094181
1102	36-37	0.037595938060682954
1102	38-39	0.04185725554730624
1102	40-41	0.027543727201880586
1102	42-43	0.09422646332600237
1102	44-45	0.12644066606107884
1102	46-47	0.044439162744062344
1102	48-49	-0.06196151158491148
1102	50-51	-0.09161680908362513
1102	52-53	-0.00515839747146174
1102	54-55	-0.03046686967595491
1102	56-57	-0.028801537113523068
1102	58-59	-0.07241215452051009
1102	60-61	-0.08679054017101251
1102	62-63	-0.0868490917646767
1102	64-65	-0.1493300708260854
1102	66-67	-0.1230022701292377
1102	68-69	0.19897057836629983
1102	70-71	0.1611854026512276
1102	72-73	0.15501293729038323
1102	74-75	0.10805714713162118
1102	76-77	0.26175901110077504
1102	78-79	0.2510461780423512
1102	80-81	0.21243214052253023
1102	82-83	0.2299284820684484
1102	84-85	0.202189928889144
1102	86-87	0.17945488686436306
1102	88-89	0.22730900269367638
1102	90-91	0.3285944257356981
1102	92-93	0.35539243341862203
1102	94-95	0.3732753927250734
1102	96-97	0.3784444024649183
1102	98-99	0.41970565058983667
1102	100-101	0.5520345456393869
1103	1	0.15178837437652248
1103	2	0.14197865349312622
1103	3	0.10802758683252023
1103	4	0.09418081967476866
1103	5	0.06247797609402994
1103	6	0.15287223096515135
1103	7	-0.005789865404771888
1103	8	0.0029209607628288836
1103	9	-0.0022671998118752867
1103	10-11	-0.002533863751274623
1103	12-13	0.025553969760025552
1103	14-15	0.02596562716247064
1103	16-17	0.08848486273358702
1103	18-19	0.133529929343581
1103	20-21	0.11802538861142864
1103	22-23	0.08428998622841277
1103	24-25	0.11110773167281707
1103	26-27	0.05985567652765411
1103	28-29	0.08604423238001147
1103	30-31	0.16534188818653917
1103	32-33	0.09078504904444884
1103	34-35	0.0660403229439126
1103	36-37	0.06626165176064802
1103	38-39	0.019818688776652493
1103	40-41	0.007471746562352166
1103	42-43	0.12303742201991952
1103	44-45	0.09674650314776301
1103	46-47	0.09961616052135724
1103	48-49	0.11327302024953667
1103	50-51	0.08598902714085455
1103	52-53	0.10793157649180074
1103	54-55	0.1277801925090145
1103	56-57	0.09292205847604862
1103	58-59	0.12181387797184584
1103	60-61	0.14620472988251976
1103	62-63	0.13284291685473448
1103	64-65	0.15067084006861364
1103	66-67	0.14683692262805792
1103	68-69	0.3559655195876452
1103	70-71	0.32402086257641116
1103	72-73	0.34314877332486304
1103	74-75	0.35726138920256645
1103	76-77	0.45743677822882844
1103	78-79	0.4290174143667578
1103	80-81	0.40221811858792833
1103	82-83	0.39240476447806216
1103	84-85	0.4496145305982395
1103	86-87	0.39272486551541874
1103	88-89	0.4291646205349515
1103	90-91	0.4936250592601681
1103	92-93	0.5215318082111544
1103	94-95	0.6218681294694832
1103	96-97	0.6054452812667428
1103	98-99	0.596633754812796
1103	100-101	0.7520842033713038
1104	1	0.16627394036299847
1104	2	0.1811878308185655
1104	3	0.12030552118674365
1104	4	0.12375227741684114
1104	5	0.08861905587551178
1104	6	0.21960958949080833
1104	7	0.07562783447187371
1104	8	0.10876553452364845
1104	9	0.11448063878801662
1104	10-11	0.09836739396003935
1104	12-13	0.08992355550729059
1104	14-15	0.10040578393142852
1104	16-17	0.1136374167319758
1104	18-19	0.15168099704338545
1104	20-21	0.13960395875409404
1104	22-23	0.19022722989721075
1104	24-25	0.16793214219680408
1104	26-27	0.13924514103211294
1104	28-29	0.12909691907412935
1104	30-31	0.17426362740780377
1104	32-33	0.1187935584469173
1104	34-35	0.10057882282890773
1104	36-37	0.14458804411848547
1104	38-39	0.10802302830975918
1104	40-41	0.0685266304469323
1104	42-43	0.08571757453704976
1104	44-45	0.12572100115588825
1104	46-47	0.11311169435487756
1104	48-49	0.1459453439443621
1104	50-51	0.05889237943335246
1104	52-53	0.021901195950619012
1104	54-55	0.050653943763215636
1104	56-57	0.03269093054602479
1104	58-59	0.07790923108193937
1104	60-61	0.16604029696472367
1104	62-63	0.16905450723928084
1104	64-65	0.1370847376670028
1104	66-67	0.07911489589598375
1104	68-69	0.21576228561328037
1104	70-71	0.19538042845515946
1104	72-73	0.14953054356649176
1104	74-75	0.14606722191583899
1104	76-77	0.2609464059906017
1104	78-79	0.24864245217960956
1104	80-81	0.2139133028535234
1104	82-83	0.21845372119148365
1104	84-85	0.13975271008010992
1104	86-87	0.0874114381788722
1104	88-89	0.07953684340232314
1104	90-91	0.10242461662705438
1104	92-93	0.05887488410168018
1104	94-95	-0.005081449023450091
1104	96-97	-0.06410718966113294
1104	98-99	-0.09220648375686835
1104	100-101	-0.048493123376196934
1105	1	0.11440573884809169
1105	2	0.11368929273654516
1105	3	0.06544957741400026
1105	4	0.06120052874873494
1105	5	0.07774410081772487
1105	6	0.1426272531211623
1105	7	0.038389927373259525
1105	8	0.09938232433875527
1105	9	0.08936073244688458
1105	10-11	0.0526300850384942
1105	12-13	0.01815965051436308
1105	14-15	0.04485088754797317
1105	16-17	0.027115196223626015
1105	18-19	0.005698048828023161
1105	20-21	0.026271790696860364
1105	22-23	0.06698202448670543
1105	24-25	0.025083983989190983
1105	26-27	0.01690471522572068
1105	28-29	0.03284587617953605
1105	30-31	0.05065367666871623
1105	32-33	0.04594056261117174
1105	34-35	-0.004508877709334058
1105	36-37	-0.01662838035904457
1105	38-39	0.00386709030198773
1105	40-41	-0.01378410438044142
1105	42-43	-0.023301662437276605
1105	44-45	0.00834242271100294
1105	46-47	-0.0017939960608472916
1105	48-49	-0.013164003174850336
1105	50-51	-0.14416381423198033
1105	52-53	-0.062326897416888016
1105	54-55	-0.010544518231291988
1105	56-57	0.007179329356219455
1105	58-59	-0.020080425541831204
1105	60-61	0.07249650257052309
1105	62-63	0.07567346115596152
1105	64-65	0.07006720132627109
1105	66-67	-0.04383277307280764
1105	68-69	0.07670949190198684
1105	70-71	0.08231567613086099
1105	72-73	0.07975834820294736
1105	74-75	0.0837938135042684
1105	76-77	0.21833751966879333
1105	78-79	0.20509888437040402
1105	80-81	0.18533633286572737
1105	82-83	0.10447886476601909
1105	84-85	0.08243395869182635
1105	86-87	0.040333576822114026
1105	88-89	0.0643135997592843
1105	90-91	0.16660889631252473
1105	92-93	0.16206760801067688
1105	94-95	0.2308433612024352
1105	96-97	0.20913945152574698
1105	98-99	0.178326800684939
1105	100-101	0.276533063518265
1106	1	0.04713003358549628
1106	2	0.04985615914653607
1106	3	0.029032614191649486
1106	4	0.045766081755900245
1106	5	0.06809153286028646
1106	6	0.12951744151788347
1106	7	0.05430975030496654
1106	8	0.07767583651678933
1106	9	0.0958150988197346
1106	10-11	0.05710023060854752
1106	12-13	0.032661350420575275
1106	14-15	-0.03664000983353333
1106	16-17	-0.00505851076696473
1106	18-19	-0.058662411256008795
1106	20-21	-0.05591490497020146
1106	22-23	-0.03593199378796896
1106	24-25	-0.025469786192935828
1106	26-27	-0.06034150939039762
1106	28-29	0.003842460307737383
1106	30-31	-0.010390831675671564
1106	32-33	-0.019922995084975526
1106	34-35	-0.04129428291907544
1106	36-37	0.006863742807531992
1106	38-39	0.03214171789891651
1106	40-41	0.023258741553071616
1106	42-43	-0.010223180413156285
1106	44-45	-0.04274254564619895
1106	46-47	-0.011065496296268407
1106	48-49	0.003683976041415349
1106	50-51	-0.18102806427018692
1106	52-53	-0.15595565709179482
1106	54-55	-0.10583535049006088
1106	56-57	-0.05358850446052088
1106	58-59	-0.026020593272995995
1106	60-61	0.005733927960879015
1106	62-63	-0.02387114114045019
1106	64-65	-0.04545032305199115
1106	66-67	-0.10043811389548196
1106	68-69	-0.07001784897683905
1106	70-71	-0.11063992411775558
1106	72-73	-0.09104430821817289
1106	74-75	-0.058013023693884946
1106	76-77	0.11513088483075862
1106	78-79	0.0841767060498455
1106	80-81	0.07976110425774507
1106	82-83	0.058729728347483245
1106	84-85	0.10787087883471713
1106	86-87	0.0850115451635709
1106	88-89	0.05434763053484559
1106	90-91	0.1581976323460701
1106	92-93	0.14677343585725566
1106	94-95	0.2000976008129456
1106	96-97	0.1625152938037644
1106	98-99	0.15497580084148055
1106	100-101	0.2742457719838072
1107	1	-0.04479449266787583
1107	2	-0.02791093257219046
1107	3	-0.02054242259705319
1107	4	-0.06829980055683649
1107	5	-0.026589369270439533
1107	6	0.03945703164571057
1107	7	0.038602004375135834
1107	8	0.011695510915608054
1107	9	-0.04063596552472859
1107	10-11	-0.06675394921802535
1107	12-13	-0.08209434233449997
1107	14-15	-0.11633917860006449
1107	16-17	-0.14083592967221392
1107	18-19	-0.2201273710487328
1107	20-21	-0.20501679489439795
1107	22-23	-0.16141809967347598
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2308	18-19	-0.8450625027652165
2308	20-21	-0.8993605612305018
2308	22-23	-0.872610476064807
2308	24-25	-0.7312139009010821
2308	26-27	-0.7392689988807533
2308	28-29	-0.7212174934184503
2308	30-31	-0.803449017787635
2308	32-33	-0.8026177091791098
2308	34-35	-0.7285860587929349
2308	36-37	-0.7391314197755818
2308	38-39	-0.7261158195866457
2308	40-41	-0.6171054006021777
2308	42-43	-0.8029509385146056
2308	44-45	-0.7516206524358822
2308	46-47	-0.6804144694721614
2308	48-49	-0.7520062796011189
2308	50-51	-0.657925705932449
2308	52-53	-0.6157351120631347
2308	54-55	-0.639452929518125
2308	56-57	-0.7384099878540269
2308	58-59	-0.8309890562026254
2308	60-61	-0.6528014646345177
2308	62-63	-0.6366289782351942
2308	64-65	-0.6177536254218658
2308	66-67	-0.24134714236749844
2308	68-69	-0.5674288437529924
2308	70-71	-0.6852774559071477
2308	72-73	-0.6723156672286095
2308	74-75	-0.7080963861635361
2308	76-77	-0.5912062171485566
2308	78-79	-0.6488408001519232
2308	80-81	-0.7809297982027985
2308	82-83	-0.7465961091391797
2308	84-85	-0.8997449040642529
2308	86-87	-0.9898043312944722
2308	88-89	-1.028156778945263
2308	90-91	-1.0501415967613
2308	92-93	-1.3012947561257384
2308	94-95	-1.506241183621377
2308	96-97	-1.7993912286800011
2308	98-99	-2.0760947975080626
2308	100-101	-2.358371821294213
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	395372.0
3	120369.0
4	71477.0
5	62287.0
6	97448.0
7	163463.0
8	243147.0
9	431660.0
10	679958.0
11	692406.0
12	531048.0
13	415022.0
14	357643.0
15	341650.0
16	333781.0
17	341203.0
18	367736.0
19	386035.0
20	424199.0
21	463617.0
22	501604.0
23	539151.0
24	732696.0
25	1250104.0
26	443584.0
27	431835.0
28	417429.0
29	436813.0
30	482689.0
31	544199.0
32	628626.0
33	748932.0
34	983968.0
35	2010522.0
36	2466852.0
37	4344169.0
38	7669472.0
39	3378173.0
40	7173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.00068527817369	19.300056621152095	9.02165505398959	35.67760304668462
2	17.089764960683425	22.360514037046404	47.90464705683342	12.64507394543675
3	17.229617848272255	23.25695879421417	41.28944960335389	18.22397375415968
4	17.26442664526945	25.563195827542234	36.88114513332665	20.291232393861673
5	22.644113303337463	27.64960891587543	34.590308175608534	15.115969605178572
6	22.6633980249614	30.37268280594362	35.69814799437883	11.265771174716157
7	13.91992157490048	23.31080914382682	51.80214266772253	10.967126613550178
8	10.562361746751089	30.030441677138636	45.81707648591152	13.59012009019875
9	17.91197638951944	23.6045006919025	45.356649296938336	13.12687362163972
10-11	17.77788604782359	34.24189356964591	37.32375212292261	10.65646825960789
12-13	17.50835091136806	24.259073678669306	42.267508094130186	15.96506731583245
14-15	15.067333144433942	25.859521410361047	45.18694812714402	13.886197318060988
16-17	18.95221743076654	25.728054895964465	42.086369871553984	13.233357801715014
18-19	18.542965415195493	26.16053452866601	43.51165149826793	11.784848557870571
20-21	17.442549041824034	30.780492462250685	40.162103901405935	11.614854594519354
22-23	23.098759200323208	26.62509366363449	38.86724827473889	11.40889886130341
24-25	15.905368095515648	34.361397781112686	37.74884739179932	11.984386731572345
26-27	16.897424565933786	33.99617023929941	37.15822772503073	11.948177469736072
28-29	18.545316690637982	33.0560859376504	36.29686725672705	12.10173011498457
30-31	19.37603955763304	28.797301974747263	39.718318926832026	12.108339540787668
32-33	15.479387747905527	31.942134426693336	40.25197385871034	12.326503966690794
34-35	18.065350029135338	34.00457099569369	35.66066053996806	12.269418435202908
36-37	13.278808858834227	31.43693079185766	40.35711531674002	14.927145032568095
38-39	13.331015128739443	29.121097406247138	40.63328697019836	16.91460049481506
40-41	20.490746628517034	28.868394977765018	36.74539614369544	13.895462250022511
42-43	17.95544771257161	29.056504009698404	40.971032718907004	12.017015558822987
44-45	20.17060254831161	28.241605900464656	39.249038531025796	12.338753020197933
46-47	15.816246832818312	29.52857924367177	37.90357872331446	16.751595200195453
48-49	14.516324638552708	29.293146193304054	36.53612826322741	19.654400904915825
50-51	17.677938246181476	29.56309236734804	38.95845191684271	13.80051746962777
52-53	13.106441443582998	34.53426642978035	40.54609286566653	11.81319926097012
54-55	13.342037635650758	33.530993849819644	36.61474091228934	16.51222760224025
56-57	13.282146421874575	32.42854270790662	42.08500021409653	12.20431065612228
58-59	12.970409856317186	38.67248761159638	36.19020009209585	12.166902439990574
60-61	12.62194629085208	40.82490476139228	34.28857641608825	12.264572531667394
62-63	12.557863307495968	41.79835845208439	33.65681563129051	11.986962609129122
64-65	12.692457894540402	41.19412824817062	33.98317401651268	12.130239840776298
66-67	13.079050963903782	41.635972819129194	33.39535024703534	11.88962596993169
68-69	13.805364846815651	40.42458575756625	33.7087569372427	12.061292458375398
70-71	13.28926341406337	40.42772994253283	33.50730011913842	12.77570652426538
72-73	14.122463843447122	39.400404356211745	33.776827039086236	12.700304761254893
74-75	14.594253305730529	38.50251843920655	33.840214494953166	13.063013760109765
76-77	14.757359217718744	37.865612407249046	34.14299461687468	13.23403375815753
78-79	14.67906824471991	38.41097180701772	33.926393939246054	12.983566009016318
80-81	15.215199067409266	38.205771492829825	33.75723777926717	12.821791660493743
82-83	15.43569416162204	37.36026623761875	33.812128636979075	13.391910963780134
84-85	15.49996319142588	37.260618329089944	33.80458660021355	13.434831879270625
86-87	16.375155735188013	37.50493595537083	32.98813607563126	13.131772233809894
88-89	16.44860257937085	37.25501689988686	33.07335536657562	13.223025154166674
90-91	16.334611920848857	37.44609375733452	32.559762698614605	13.659531623202017
92-93	17.0562199388176	37.2823520447858	32.09581579081593	13.565612225580665
94-95	17.47355249566712	37.38296104198834	31.088770717273746	14.054715745070801
96-97	17.398099961305185	37.65633166815976	31.69309571930149	13.252472651233566
98-99	17.870407308020944	37.5599171692367	30.942530880677577	13.627144642064785
100-101	18.73753775025537	37.687524801422605	29.78066096979087	13.794276478531156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	364035.0
1	374500.5
2	315463.5
3	269237.5
4	319660.0
5	382707.0
6	397208.5
7	373794.5
8	372924.5
9	370912.5
10	367292.0
11	363990.5
12	368126.5
13	369060.5
14	410515.0
15	430320.5
16	392907.5
17	386217.0
18	397020.0
19	416490.0
20	445938.0
21	485722.5
22	535471.5
23	591765.0
24	663757.5
25	768254.0
26	876602.5
27	965791.0
28	1032480.0
29	1079667.0
30	1109765.0
31	1105738.0
32	1094881.5
33	1106077.5
34	1127213.0
35	1122147.5
36	1116015.0
37	1128344.0
38	1141082.0
39	1135948.5
40	1110062.0
41	1057896.0
42	950840.0
43	847254.5
44	770591.5
45	694834.0
46	619118.0
47	542682.5
48	465211.5
49	383499.5
50	304700.0
51	239385.5
52	190796.5
53	156805.5
54	144638.0
55	168201.0
56	140142.5
57	73863.5
58	48309.0
59	33453.5
60	23859.5
61	17571.0
62	13261.0
63	9926.5
64	7300.0
65	5590.5
66	4406.0
67	3422.0
68	2715.0
69	2173.0
70	1809.5
71	1599.5
72	1411.5
73	1235.5
74	1054.0
75	910.0
76	768.5
77	633.0
78	536.0
79	469.0
80	374.0
81	297.5
82	228.0
83	166.0
84	137.0
85	102.5
86	74.5
87	48.5
88	36.0
89	25.0
90	20.0
91	14.0
92	7.5
93	7.0
94	3.5
95	2.5
96	3.0
97	1.0
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1416529030458723
2	0.16913625675463095
3	0.1575155094043331
4	0.12024897479820543
5	0.13178385455724495
6	0.20859241493784675
7	0.2269308701775902
8	0.22720564646961697
9	0.24966288383671967
10-11	0.24725000452235982
12-13	0.23737523152764858
14-15	0.2577115393906699
16-17	0.270730497351958
18-19	0.28498737975388744
20-21	0.29075625075992817
22-23	0.29792905688304305
24-25	0.29088648327333666
26-27	0.27451725812645156
28-29	0.23659669870023944
30-31	0.2353959835491434
32-33	0.2839798666831227
34-35	0.29631760842042787
36-37	0.30216805363816407
38-39	0.2950496303228461
40-41	0.2950997197510801
42-43	0.3040113445971768
44-45	0.3098532030557872
46-47	0.3024743147136522
48-49	0.049050430379816407
50-51	0.01991985004541823
52-53	0.0010275488420583585
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.862253041945288E-6
78-79	2.1896235770881454E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.007154258723403E-5
94-95	0.0
96-97	3.720928954528874E-5
98-99	2.7191403898480238E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4937512E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.76079269079416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43245895051588	34.00684036534099
2	9.778058608986154	8.166789569766118
3	3.076008470731995	3.853696561840969
4	1.4952589961235698	2.4977280382464557
5	0.8669270424926113	1.8101780249788626
6	0.6045799333495921	1.5148642356975885
7	0.41440943818778286	1.2114246646087936
8	0.32264117457674657	1.0779000964011074
9	0.237125790358843	0.8912304875534726
>10	1.51157063609458	12.096140831255376
>50	0.14681746790988892	4.255365522814449
>100	0.09808677002998492	7.982959761702885
>500	0.009586565739985413	2.7649325576250177
>1k	0.005729886294392216	4.713749393614739
>5k	4.3743145043777183E-4	1.2349999620521324
>10k+	3.028371575576863E-4	11.921199926500945
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1152091	3.297575969347789	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	994481	2.8464562674067917	Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	394014	1.1277677700690305	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	373909	1.0702221726607206	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	127688	0.3654753664199099	Illumina Single End PCR Primer 1 (98% over 50bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	109082	0.3122202863214759	Illumina Single End PCR Primer 1 (97% over 44bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	108896	0.3116879072556741	Illumina Single End PCR Primer 1 (100% over 43bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93008	0.2662124309252474	No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT	79352	0.22712550338444248	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	51927	0.148628213709093	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	50856	0.14556274070116956	No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50684	0.14507043317795498	No Hit
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44015	0.12598206764122183	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40293	0.11532876181910148	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2496189196299956	1.1449012167781151E-5	0.0	4.293379562917932E-5	0.0
2	3.255764176911052	2.0035771293617015E-5	0.0	5.152055475501518E-5	0.0
3	3.2561105095291274	2.2898024335562303E-5	0.0	6.0107313880851044E-5	0.0
4	3.713000513602686	2.2898024335562303E-5	0.0	6.583181996474163E-5	0.0
5	3.7205454126212536	2.2898024335562303E-5	0.0	7.728083213252278E-5	0.0
6	3.723359007361486	2.2898024335562303E-5	0.0	8.586759125835863E-5	0.0
7	3.7482777823446614	2.2898024335562303E-5	0.0	1.3166363992948325E-4	0.0
8	4.114591788905861	2.2898024335562303E-5	0.0	1.3738814601337382E-4	0.0
9	4.116935974147214	2.2898024335562303E-5	0.0	1.7459743555866256E-4	0.0
10-11	4.2098919350639505	2.2898024335562303E-5	0.0	1.8032194164255315E-4	0.0
12-13	4.258550236777021	2.2898024335562303E-5	0.0	1.9177095381033428E-4	0.0
14-15	4.270620357854904	2.2898024335562303E-5	0.0	2.017888394571428E-4	0.0
16-17	4.2867992431744995	2.7191403898480234E-5	0.0	2.1610010466686924E-4	0.0
18-19	4.2948922636506	2.862253041945288E-5	0.0	2.561716472541033E-4	0.0
20-21	4.3190754396019955	2.862253041945288E-5	0.0	2.93380936799392E-4	0.0
22-23	4.342902265049669	2.862253041945288E-5	0.0	3.119855815720364E-4	0.0
24-25	4.348967379245551	2.862253041945288E-5	0.0	3.3631473242857134E-4	0.0
26-27	4.355114067653129	3.1484783461398166E-5	0.0	3.9928429935136766E-4	0.0
28-29	4.362906551559825	3.1484783461398166E-5	0.0	4.5652936019027345E-4	0.0
30-31	4.372042863269714	3.864041606626138E-5	0.0	4.923075232145895E-4	0.0
32-33	4.381951983300929	4.007154258723403E-5	0.0	5.06618788424316E-4	0.0
34-35	4.39191548613994	4.5796048671124605E-5	0.0	5.252234331969603E-4	0.0
36-37	4.403045357093545	4.5796048671124605E-5	1.431126520972644E-6	5.409658249276594E-4	0.0
38-39	4.415808143407579	4.722717519209725E-5	5.724506083890576E-6	5.495525840534953E-4	0.0
40-41	4.430475759121027	4.865830171306989E-5	5.724506083890576E-6	5.567082166583585E-4	0.0
42-43	4.447381656713278	5.152055475501518E-5	5.724506083890576E-6	5.824684940358661E-4	0.0
44-45	4.472875744557884	5.438280779696047E-5	5.724506083890576E-6	6.139532774972643E-4	0.0
46-47	4.586540106233095	5.438280779696047E-5	5.724506083890576E-6	6.354201753118538E-4	0.0
48-49	4.615420239426323	5.438280779696047E-5	5.724506083890576E-6	6.411446813957445E-4	0.0
50-51	4.6388835587376684	5.724506083890576E-5	5.724506083890576E-6	6.55455946605471E-4	0.0
52-53	4.6651017966018875	6.296956692279633E-5	5.724506083890576E-6	6.640427057313068E-4	0.0
54-55	4.694457063800078	6.869407300668691E-5	5.724506083890576E-6	6.683360852942247E-4	0.0
56-57	4.725077446842809	6.869407300668691E-5	5.724506083890576E-6	6.726294648571427E-4	0.0
58-59	4.76087707676494	6.869407300668691E-5	5.724506083890576E-6	6.840784770249238E-4	0.0
60-61	4.802056311279406	7.15563260486322E-5	5.724506083890576E-6	6.940963626717323E-4	0.0
62-63	4.844180089297716	7.15563260486322E-5	5.724506083890576E-6	7.026831217975682E-4	0.0
64-65	4.889503866216919	7.441857909057748E-5	5.724506083890576E-6	7.055453748395135E-4	0.0
66-67	4.938694546995791	7.584970561155013E-5	5.724506083890576E-6	7.15563260486322E-4	2.862253041945288E-6
68-69	4.994326728245561	7.728083213252278E-5	5.724506083890576E-6	7.227188930911852E-4	2.862253041945288E-6
70-71	5.0594200869254795	7.728083213252278E-5	5.724506083890576E-6	7.298745256960485E-4	2.862253041945288E-6
72-73	5.1308561983463505	7.728083213252278E-5	5.724506083890576E-6	7.413235378638295E-4	2.862253041945288E-6
74-75	5.2087624327685385	7.728083213252278E-5	5.724506083890576E-6	7.542036765525833E-4	2.862253041945288E-6
76-77	5.304514814907256	7.728083213252278E-5	5.724506083890576E-6	7.656526887203645E-4	2.862253041945288E-6
78-79	5.411304044775713	7.728083213252278E-5	5.724506083890576E-6	7.713771948042551E-4	2.862253041945288E-6
80-81	5.528596312181589	8.014308517446806E-5	5.724506083890576E-6	7.79963953930091E-4	2.862253041945288E-6
82-83	5.665676765992954	8.586759125835865E-5	5.724506083890576E-6	7.842573334930089E-4	2.862253041945288E-6
84-85	5.827019107714367	9.731660342613979E-5	5.724506083890576E-6	7.914129660978721E-4	2.862253041945288E-6
86-87	6.014941762309806	9.874772994711243E-5	5.724506083890576E-6	7.985685987027353E-4	5.724506083890576E-6
88-89	6.234855819155067	1.0017885646808508E-4	5.724506083890576E-6	8.014308517446806E-4	5.724506083890576E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	15905	0.0	66.56663	9
ACCACCG	21240	0.0	59.367676	1
TCGGGAG	10415	0.0	58.210323	3
ATCGGGA	11155	0.0	58.18407	2
CCACCGA	22000	0.0	57.080364	2
CACCGAG	22785	0.0	55.052223	3
ACCGAGA	23685	0.0	53.18572	4
AGAGCGG	21005	0.0	50.01253	8
CGCGTGT	98350	0.0	46.145844	1
GATCGGG	15740	0.0	43.530167	1
CCGAGAT	43685	0.0	42.873077	1
GTATCAT	324325	0.0	40.889996	50-51
CGCCGTA	327670	0.0	40.67639	46-47
CCGTATC	328115	0.0	40.671562	48-49
GAGCGTC	397405	0.0	40.58922	9
GTCGCCG	331300	0.0	40.386456	44-45
GGCGCCG	16790	0.0	40.084785	44-45
ATACGGC	1690	0.0	39.95146	1
ATCATTA	342890	0.0	39.880753	52-53
AGAGCGT	404505	0.0	39.876053	8
>>END_MODULE