FastQCFastQC Report
Fri 27 May 2016
SRR1233052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34937512
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15038954.304528038516309TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG9212792.636933620230313TruSeq Adapter, Index 1 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3577771.0240483065880592No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3366270.9635116547509164No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC1601600.4584184471979573TruSeq Adapter, Index 1 (98% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC1044940.2990882693650309TruSeq Adapter, Index 1 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG966490.2766338942509701TruSeq Adapter, Index 1 (97% over 49bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT789220.225894734576406No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT548090.15687722697597928No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT516710.14789547693035496No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447230.1280085427949191No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT388290.11113842336569357No Hit
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368860.10557706570519389No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA350090.10020461674546258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT193400.084.945591
CCGAGAT205800.069.961081
GGTAGAT180000.067.40945
GTGGTCG63250.066.583123
GTCGCCG17250.065.96086
GGTGGTA186850.063.3715442
GTGGTAG189250.062.5928343
GTAGATC199300.060.525956
CGGAGAT86550.058.568631
GGTGGTC76550.056.8033942
TCGGCAC35800.056.7078827
GATCGGC45300.053.0492745
CGCGTGT851350.052.8095441
CGGCACT34400.052.56528
GAGATCT239550.052.0316543
TGGTAGA229100.051.84964
TCGGGAG68400.051.7684363
ATCGGGA70550.051.596142
GGTCGCC22900.051.33625
CGAGATC280550.051.074992
Produced by FastQC (version 0.11.5)