FastQCFastQC Report
Fri 27 May 2016
SRR1233051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26247980
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1546890.5893367794397893Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1416930.5398243979155729No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC1018640.38808319725937Illumina Single End PCR Primer 1 (97% over 44bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT790880.3013108056315191No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT721550.2748973444813658Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG562090.21414600285431487Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA540530.20593203743678562No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT526340.20052590713647298No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT405640.1545414161394515No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC358180.13646002473333185No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA331870.12643639624839703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315980.1203825970608024No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA304120.1158641541177645No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC267970.10209166572056211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT390900.073.343561
GCGTGTT516750.055.3627662
ACCACCG175350.051.4380761
CCACCGA182000.049.663912
ACCGAGA191150.047.472434
CCGAGAT378350.046.686691
CACCGAG194450.046.551993
CGAGATC399500.044.2158052
CGTGTTT679550.042.7099233
CGCGCGT8200.041.147061
CGGAGAT171650.039.46590842-43
CGGCGAC32750.039.323511
CTCGCGT2700.038.7215921
CTCGGAG185450.037.1331140-41
CAAAACG446200.036.613189
TCTCGGA191100.036.57048440-41
TCGGAGA190100.036.461442-43
GGCGACC37450.033.881392
GTTTTTT3586900.033.7260321
GAGCGGC46850.031.7465069
Produced by FastQC (version 0.11.5)