##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233051_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26247980
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20458599099816	34.0	31.0	34.0	30.0	34.0
2	32.43267740984258	34.0	33.0	34.0	31.0	34.0
3	32.44449260476425	34.0	33.0	34.0	31.0	34.0
4	35.69109158114262	37.0	37.0	37.0	35.0	37.0
5	35.657413713360036	37.0	37.0	37.0	35.0	37.0
6	35.6304877556292	37.0	37.0	37.0	35.0	37.0
7	35.645706450553526	37.0	37.0	37.0	35.0	37.0
8	35.638497248169195	37.0	37.0	37.0	35.0	37.0
9	37.34134504826657	39.0	39.0	39.0	35.0	39.0
10-11	37.27726647917287	39.0	39.0	39.0	35.0	39.0
12-13	37.2302766727192	39.0	38.5	39.0	35.0	39.0
14-15	38.681651273736115	41.0	39.0	41.0	35.5	41.0
16-17	38.508305210534296	41.0	39.0	41.0	35.0	41.0
18-19	38.1775817415283	41.0	38.5	41.0	34.0	41.0
20-21	37.87608457869901	41.0	38.0	41.0	33.5	41.0
22-23	37.55672394599508	40.5	38.0	41.0	33.0	41.0
24-25	37.21457289284737	41.0	38.0	41.0	31.5	41.0
26-27	36.81175284726672	40.0	38.0	41.0	30.5	41.0
28-29	36.48005208400799	41.0	38.0	41.0	28.0	41.0
30-31	36.2139477018803	40.5	38.0	41.0	25.5	41.0
32-33	36.006099974169445	40.5	38.0	41.0	24.0	41.0
34-35	35.81119118499785	40.0	38.0	41.0	23.0	41.0
36-37	35.71890156880644	40.0	38.0	41.0	21.5	41.0
38-39	35.59212581311019	40.0	37.0	41.0	19.0	41.0
40-41	35.46307142492489	40.0	37.0	41.0	18.0	41.0
42-43	35.318984451374924	40.0	37.0	41.0	16.0	41.0
44-45	35.26392362383696	40.0	37.0	41.0	15.0	41.0
46-47	35.23095177228876	40.0	37.0	41.0	14.5	41.0
48-49	35.15212500161917	40.0	37.0	41.0	12.0	41.0
50-51	34.64065554758881	39.5	35.5	40.5	9.0	41.0
52-53	34.67742628194627	40.0	36.0	41.0	8.5	41.0
54-55	34.834389922576904	40.0	36.0	41.0	7.5	41.0
56-57	34.71319958716823	40.0	35.5	41.0	4.0	41.0
58-59	34.511797650714456	40.0	35.0	41.0	2.0	41.0
60-61	34.335048982816964	40.0	35.0	41.0	2.0	41.0
62-63	34.096019693705955	39.5	35.0	41.0	2.0	41.0
64-65	33.73012026068292	39.0	35.0	41.0	2.0	41.0
66-67	33.287718007252366	39.0	35.0	41.0	2.0	41.0
68-69	32.9428032557172	38.5	34.5	41.0	2.0	41.0
70-71	32.521584118092136	37.0	34.0	40.0	2.0	41.0
72-73	32.10125238208807	37.0	34.0	40.0	2.0	41.0
74-75	31.59834743473593	36.0	33.0	39.0	2.0	41.0
76-77	31.089999630447753	36.0	32.0	39.0	2.0	41.0
78-79	30.664664423700415	35.0	32.0	38.5	2.0	40.0
80-81	30.227696093185074	35.0	31.5	37.0	2.0	39.0
82-83	29.78512799842121	35.0	31.0	37.0	2.0	39.0
84-85	29.328977125096863	35.0	31.0	36.0	2.0	38.5
86-87	28.850821167952734	35.0	30.0	36.0	2.0	37.0
88-89	28.35110576128144	35.0	29.0	35.5	2.0	37.0
90-91	27.909945622482187	34.5	29.0	35.0	2.0	36.5
92-93	27.407960784029857	34.0	27.5	35.0	2.0	36.0
94-95	26.872084766142002	34.0	25.5	35.0	2.0	36.0
96-97	26.188201053947772	34.0	24.0	35.0	2.0	35.0
98-99	25.469090573827014	33.5	19.0	35.0	2.0	35.0
100-101	23.84420650655784	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3347986094867963
1101	2	0.3166378894396118
1101	3	0.3072935098640954
1101	4	0.2923577993173154
1101	5	0.2762464354878844
1101	6	0.24035064511753745
1101	7	0.19183841806142965
1101	8	0.19266530893693812
1101	9	0.25257396240118624
1101	10-11	0.27523184905799525
1101	12-13	0.26345858936595334
1101	14-15	0.32621149510706005
1101	16-17	0.3192403056311264
1101	18-19	0.3305482973249525
1101	20-21	0.33021269669784203
1101	22-23	0.29946912657778313
1101	24-25	0.3090408546119505
1101	26-27	0.31636141957813635
1101	28-29	0.3432375691630938
1101	30-31	0.33923633219577454
1101	32-33	0.3269872954767976
1101	34-35	0.3331704417802328
1101	36-37	0.34519119714348534
1101	38-39	0.2625389606554336
1101	40-41	0.2288181125529789
1101	42-43	0.27982881638258306
1101	44-45	0.29067243374675655
1101	46-47	0.3316512328466601
1101	48-49	0.33550225814103385
1101	50-51	0.24875718881212094
1101	52-53	0.28209915298616295
1101	54-55	0.29650199931508325
1101	56-57	0.2969260479153988
1101	58-59	0.27908727793465715
1101	60-61	0.2652096147740579
1101	62-63	0.2932932812877098
1101	64-65	0.2891649976857238
1101	66-67	0.3169427644453364
1101	68-69	0.30380341676129063
1101	70-71	0.2829411528757291
1101	72-73	0.2564828920424347
1101	74-75	0.31965844591939074
1101	76-77	0.34350379547875676
1101	78-79	0.27154391558640256
1101	80-81	0.2989906813286609
1101	82-83	0.2934828265594618
1101	84-85	0.3201653140667915
1101	86-87	0.30528438136504477
1101	88-89	0.3288471823875838
1101	90-91	0.36336130677054257
1101	92-93	0.3548832980705363
1101	94-95	0.36169174091063994
1101	96-97	0.4011043801882721
1101	98-99	0.36546866739744743
1101	100-101	0.3057958854703884
1102	1	-0.03837780689427461
1102	2	0.06152555957510231
1102	3	0.06665094735078725
1102	4	0.0640538486611888
1102	5	0.05976924168599851
1102	6	0.015509215002225574
1102	7	0.007874201436564476
1102	8	-0.0014578993747278446
1102	9	0.004180641882804537
1102	10-11	-0.0514817948339612
1102	12-13	-0.0022953053835124138
1102	14-15	0.010991735985811601
1102	16-17	-0.027550550503953275
1102	18-19	-0.04777958813237149
1102	20-21	-0.031782588309837934
1102	22-23	-0.05186746431090228
1102	24-25	-0.0914737388046234
1102	26-27	-0.1420414510003738
1102	28-29	-0.20195004986405252
1102	30-31	-0.1849126709190969
1102	32-33	-0.17363922073289473
1102	34-35	-0.1687450857598165
1102	36-37	-0.19050003877376298
1102	38-39	-0.20199786985110535
1102	40-41	-0.22495454036018714
1102	42-43	-0.21990096009924542
1102	44-45	-0.2054646384743748
1102	46-47	-0.168058810753287
1102	48-49	0.052895409349773104
1102	50-51	0.008762373437782855
1102	52-53	-0.14836578115292554
1102	54-55	-0.11959994546418073
1102	56-57	-0.1476368994825208
1102	58-59	-0.20186393928852908
1102	60-61	-0.22128562265002216
1102	62-63	-0.19054510690180138
1102	64-65	-0.19107913718025316
1102	66-67	-0.1879169889949992
1102	68-69	-0.19786105179848335
1102	70-71	-0.22050380692536464
1102	72-73	-0.23454320753769053
1102	74-75	-0.1961056880557699
1102	76-77	-0.16535317168694874
1102	78-79	-0.20937313616559905
1102	80-81	-0.17575852056068442
1102	82-83	-0.16061233273573094
1102	84-85	-0.09269060219979863
1102	86-87	-0.11358640643841156
1102	88-89	-0.09675275727091659
1102	90-91	-0.09997354768405131
1102	92-93	-0.09273712553840952
1102	94-95	-0.06054147034038593
1102	96-97	-0.015546041777827924
1102	98-99	0.07769026485401653
1102	100-101	0.04511625880934744
1103	1	0.2248984661272786
1103	2	0.24112324977434696
1103	3	0.2405454201617374
1103	4	0.2101624794684156
1103	5	0.21090211616694887
1103	6	0.20542248456078482
1103	7	0.1567589135632801
1103	8	0.17012311979528505
1103	9	0.22011335712632274
1103	10-11	0.21291998795764755
1103	12-13	0.2254150725446067
1103	14-15	0.24089685143113115
1103	16-17	0.3049161893195418
1103	18-19	0.3154963712372165
1103	20-21	0.29165373142607365
1103	22-23	0.2727933331662129
1103	24-25	0.3069003796626788
1103	26-27	0.32891101226355346
1103	28-29	0.3166310567768491
1103	30-31	0.34562514015944856
1103	32-33	0.34626389629345056
1103	34-35	0.39097283363464186
1103	36-37	0.3861304697614898
1103	38-39	0.35067351134287605
1103	40-41	0.43525090318718185
1103	42-43	0.4338519927528708
1103	44-45	0.44243516630934465
1103	46-47	0.4214175479446354
1103	48-49	0.4351408910271459
1103	50-51	0.44935259116663673
1103	52-53	0.47432529795846534
1103	54-55	0.5134342670428609
1103	56-57	0.51786265163976
1103	58-59	0.5343746003140097
1103	60-61	0.49752319237880016
1103	62-63	0.5019654566164959
1103	64-65	0.5593612130029371
1103	66-67	0.5484937267344421
1103	68-69	0.5474608127188461
1103	70-71	0.5108084508740447
1103	72-73	0.5468630796857994
1103	74-75	0.5937708724003805
1103	76-77	0.6198930075223856
1103	78-79	0.5602078696891652
1103	80-81	0.5499495314674725
1103	82-83	0.580874432615353
1103	84-85	0.6369166458798432
1103	86-87	0.6204922637399655
1103	88-89	0.688982147376386
1103	90-91	0.7557926871388716
1103	92-93	0.8558505899460478
1103	94-95	0.8887046830844447
1103	96-97	1.0383206873790058
1103	98-99	1.1594280448000127
1103	100-101	1.1639145069391859
1104	1	0.14761826935881572
1104	2	0.1464569628883794
1104	3	0.13516269060688302
1104	4	0.17573138622602613
1104	5	0.1403003909096725
1104	6	0.10892546119478652
1104	7	0.12125828748935419
1104	8	0.10993284214004717
1104	9	0.14599631312871253
1104	10-11	0.1630967339052205
1104	12-13	0.17915841433536173
1104	14-15	0.15177561241360138
1104	16-17	0.20408501070902219
1104	18-19	0.22200706683018723
1104	20-21	0.2282469976054884
1104	22-23	0.1880017532905427
1104	24-25	0.19790463785965784
1104	26-27	0.21630743203322567
1104	28-29	0.24929246188016663
1104	30-31	0.2622931600463545
1104	32-33	0.2688950861542736
1104	34-35	0.24438816440498812
1104	36-37	0.2292676056254166
1104	38-39	0.2516058071820666
1104	40-41	0.2509988061741524
1104	42-43	0.23864734162534518
1104	44-45	0.2750071517288504
1104	46-47	0.3173648525868984
1104	48-49	0.32228088603490335
1104	50-51	0.287445837832486
1104	52-53	0.25679842814104603
1104	54-55	0.27698514534845486
1104	56-57	0.34533248060979815
1104	58-59	0.3153834343184556
1104	60-61	0.3190397561406684
1104	62-63	0.3312954339642147
1104	64-65	0.3961379697557277
1104	66-67	0.38097657355162085
1104	68-69	0.35764711092431867
1104	70-71	0.4015400233615978
1104	72-73	0.40131042300647124
1104	74-75	0.46096033922412616
1104	76-77	0.50251569286905
1104	78-79	0.4882511170169046
1104	80-81	0.477085010221181
1104	82-83	0.4550346330646988
1104	84-85	0.49575770708185374
1104	86-87	0.4834341177889705
1104	88-89	0.5546932298476186
1104	90-91	0.6071047554982698
1104	92-93	0.682929315891176
1104	94-95	0.6669782206873549
1104	96-97	0.7861498268667084
1104	98-99	0.8161282076688252
1104	100-101	0.788425686064361
1105	1	0.07813259421867258
1105	2	0.08388741410979605
1105	3	0.10170647982062775
1105	4	0.1028021479900616
1105	5	0.08015101895097132
1105	6	0.035899814845443245
1105	7	0.08218492106516351
1105	8	0.08607351057018775
1105	9	0.14169228776225395
1105	10-11	0.10145284184940095
1105	12-13	0.12161667532645026
1105	14-15	0.1113514385770884
1105	16-17	0.1385849289879033
1105	18-19	0.10900388019987162
1105	20-21	0.11653120202058886
1105	22-23	0.13997384916589795
1105	24-25	0.13529744344522499
1105	26-27	0.14848830664671908
1105	28-29	0.13526063330373717
1105	30-31	0.14991784245814443
1105	32-33	0.16268670298575216
1105	34-35	0.1586243013803923
1105	36-37	0.1715425769335326
1105	38-39	0.18481187801570087
1105	40-41	0.22883429318721937
1105	42-43	0.24240807894675953
1105	44-45	0.20740470707102077
1105	46-47	0.19541390713965967
1105	48-49	0.19388042796560967
1105	50-51	0.1827470610513231
1105	52-53	0.24069599670610842
1105	54-55	0.19298628518355798
1105	56-57	0.17690275353739082
1105	58-59	0.2172273360902679
1105	60-61	0.22921618260583188
1105	62-63	0.2822008567639145
1105	64-65	0.25864529610047526
1105	66-67	0.28266870019211154
1105	68-69	0.25588147350446633
1105	70-71	0.29452947348888614
1105	72-73	0.30096401936786066
1105	74-75	0.3474659706228529
1105	76-77	0.3789741781558682
1105	78-79	0.3523815640121235
1105	80-81	0.3564889487200844
1105	82-83	0.34980938907335
1105	84-85	0.421099598674342
1105	86-87	0.4697634616135353
1105	88-89	0.4499849162570051
1105	90-91	0.5369982740984902
1105	92-93	0.6121898103119214
1105	94-95	0.6061876424544543
1105	96-97	0.6762997035708196
1105	98-99	0.726198832576582
1105	100-101	0.7183334084610067
1106	1	-0.03431675933808265
1106	2	0.022105624463279128
1106	3	-0.006972146082496522
1106	4	-0.0013091596916297021
1106	5	-0.005452199040817618
1106	6	-0.02806716002060483
1106	7	0.0058288600580027605
1106	8	0.02036482531033812
1106	9	0.02302551977957279
1106	10-11	-0.04310247437776127
1106	12-13	-0.01995397453474368
1106	14-15	-0.06060918287653294
1106	16-17	-0.08420327960745766
1106	18-19	-0.0760497710433441
1106	20-21	-0.0303106972294529
1106	22-23	-0.03007772720774682
1106	24-25	-0.04443364626217061
1106	26-27	0.008386914578565552
1106	28-29	0.02419429811314444
1106	30-31	0.012756402056801619
1106	32-33	0.025025788219807055
1106	34-35	0.020829844803323283
1106	36-37	0.04346116476781958
1106	38-39	0.03868062397208405
1106	40-41	0.06348937809484312
1106	42-43	0.038795927697997
1106	44-45	0.04979190705896741
1106	46-47	0.03741955226821858
1106	48-49	0.03586558166021803
1106	50-51	-0.10673772241755586
1106	52-53	-0.09512926615832384
1106	54-55	-0.033916454124451434
1106	56-57	-0.1083225374074459
1106	58-59	-0.07679992482773201
1106	60-61	-0.031360201132642374
1106	62-63	0.0016507036438540013
1106	64-65	-0.002099643791908079
1106	66-67	-0.009955741340441193
1106	68-69	0.0036104949791777585
1106	70-71	0.005695937952431507
1106	72-73	0.006069018338138221
1106	74-75	0.04036888749770995
1106	76-77	0.09728305712890162
1106	78-79	0.1624053331318187
1106	80-81	0.16827907083131777
1106	82-83	0.13834893582062335
1106	84-85	0.21733067093902392
1106	86-87	0.2490553580706525
1106	88-89	0.13455597435234523
1106	90-91	0.2334991071991439
1106	92-93	0.3161146923071634
1106	94-95	0.3729116751006103
1106	96-97	0.38951981556310145
1106	98-99	0.41782048657700344
1106	100-101	0.360835137129321
1107	1	-0.2458090700444444
1107	2	-0.27580739806817434
1107	3	-0.2854247826698071
1107	4	-0.19221329004319898
1107	5	-0.16165464492087267
1107	6	-0.21969353699025618
1107	7	-0.16600441163835455
1107	8	-0.13867166253219665
1107	9	-0.2050949712270551
1107	10-11	-0.21398415228958356
1107	12-13	-0.2662035692865743
1107	14-15	-0.38783311588964153
1107	16-17	-0.4346544306336284
1107	18-19	-0.33084110910195363
1107	20-21	-0.3794137618762434
1107	22-23	-0.41039221186454
1107	24-25	-0.40383410497283023
1107	26-27	-0.39454569785656446
1107	28-29	-0.468216447957964
1107	30-31	-0.4770369074263954
1107	32-33	-0.37508098203234397
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2308	52-53	-1.1498086428432401
2308	54-55	-1.1428121509924978
2308	56-57	-1.2288857706679863
2308	58-59	-1.135683387222926
2308	60-61	-1.150309954259363
2308	62-63	-1.085426324185228
2308	64-65	-1.1788602944564062
2308	66-67	-1.1396004969799733
2308	68-69	-1.1188860053744705
2308	70-71	-1.1561278115919542
2308	72-73	-1.1388686278449676
2308	74-75	-1.1520628479933919
2308	76-77	-1.1300784176664038
2308	78-79	-1.2763833102382058
2308	80-81	-1.4525849160276252
2308	82-83	-1.5129606072784938
2308	84-85	-1.7112566384050716
2308	86-87	-1.9262052025486405
2308	88-89	-2.098631065589199
2308	90-91	-2.2783485917912145
2308	92-93	-2.5476917409282294
2308	94-95	-2.8071497988323664
2308	96-97	-3.2805921641446147
2308	98-99	-3.703927777746838
2308	100-101	-4.03908472286254
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	201227.0
3	57703.0
4	45657.0
5	46523.0
6	63664.0
7	97924.0
8	157496.0
9	266350.0
10	381504.0
11	371428.0
12	276125.0
13	213317.0
14	169093.0
15	149275.0
16	140373.0
17	135443.0
18	139436.0
19	139190.0
20	147710.0
21	155753.0
22	169424.0
23	187088.0
24	225793.0
25	264012.0
26	218423.0
27	241226.0
28	241878.0
29	269963.0
30	312496.0
31	369925.0
32	454972.0
33	571734.0
34	765083.0
35	1171650.0
36	1859856.0
37	4077178.0
38	7779751.0
39	3703667.0
40	8670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.928656004956004	17.47391919336799	9.113134320159544	46.48429048151647
2	14.94714384533557	18.53356169602607	52.720516779274064	13.798777679364296
3	18.87395595330253	22.981780617977282	37.423614377329095	20.720649051391096
4	19.50977044855133	30.09923813282015	33.219528887185895	17.17146253144263
5	21.20055157297695	32.109646918642454	32.1857404118405	14.504061096540102
6	16.58429226326158	34.883202935650445	33.64985890037869	14.882645900709285
7	13.945622548500989	18.96958665657341	52.289065353429244	14.795725441496355
8	13.502099578069762	23.331634511597272	44.41141893611737	18.754846974215596
9	15.812054390182793	21.773441917277413	44.21087883801924	18.20362485452055
10-11	18.30587007034262	32.87187686887989	33.8639599967527	14.958293064024792
12-13	17.80387129316003	26.071188440949385	39.0955867499183	17.029353515972293
14-15	17.945847451066793	27.695156813912508	38.26364207825862	16.095353656762075
16-17	19.21278036762028	27.77980775609968	36.22250997660789	16.784901899672146
18-19	18.840833460860434	28.110777442250274	36.166410091179046	16.88197900571025
20-21	19.370841094535376	28.50139522106037	35.38360022802693	16.74416345637732
22-23	19.413132793440766	29.126299985121474	35.17321834402175	16.28734887741602
24-25	19.09486250055557	29.806369502519075	33.93723850754939	17.161529489375965
26-27	19.08913393700509	30.249864564250906	33.57344858590489	17.087552912839108
28-29	19.546297906096374	30.436058501196943	32.645574341233	17.372069251473683
30-31	19.671316059867188	30.28553257393064	32.875513831186616	17.167637535015558
32-33	18.6943159550069	30.347607281941208	33.19703604254485	17.76104072050704
34-35	19.430848074698513	30.994942619133823	32.11206076406852	17.462148542099147
36-37	18.212417904069554	31.258433885293506	32.88114245530547	17.648005755331468
38-39	18.664533428740516	30.072132703909826	32.93404628397042	18.329287583379237
40-41	18.966661657220364	29.996052434038756	33.07362926971711	17.96365663902377
42-43	18.794849648494008	30.130147859965	33.36405533299828	17.710947158542716
44-45	19.943671027459136	29.30590713478411	32.838889577972715	17.911532259784043
46-47	18.350427717022125	30.90771115294978	32.42434625911315	18.31751487091495
48-49	18.293688122451897	30.078519081400724	33.134086714299535	18.49370608184784
50-51	18.901831691142682	29.687691551807195	33.43555526983089	17.974921487219234
52-53	18.860450895204004	30.756381782978558	32.84133281418397	17.541834507633464
54-55	18.705602618433	31.24865498568297	31.96100619856529	18.08473619731874
56-57	19.109065824090703	30.669361238920377	32.33887833704046	17.88269459994846
58-59	18.315955251066583	31.90760000600624	31.725524397037823	18.05092034588935
60-61	18.059611037774637	31.888328301742842	31.76239150234048	18.28966915814204
62-63	18.49297249625225	32.72040135093346	30.84638256703386	17.94024358578043
64-65	18.450893729447802	31.83483192430377	31.635754071394768	18.078520274853663
66-67	18.813191695230206	32.33216716062795	30.993211870911164	17.861429273230687
68-69	19.46366287130024	31.82008556772994	31.13753513789988	17.578716423069938
70-71	18.065898372493557	32.71112388982221	30.746491068983755	18.476486668700478
72-73	18.640231376314535	31.998922843696455	31.15224109549487	18.208604684494144
74-75	19.097783175462734	31.918622810865955	30.66709156413386	18.316502449537456
76-77	18.744933947256783	31.93686526123801	31.08446594950374	18.233734842001468
78-79	18.444809634952357	32.83347676141202	30.60053365763246	18.121179946003156
80-81	18.760580177233393	32.82011712016795	30.803875267726244	17.615427434872416
82-83	18.86114904855731	31.960427784839435	30.907086228720686	18.271336937882566
84-85	18.64149233706452	31.915497996695102	30.996248800737945	18.446760865502434
86-87	19.485587430679875	32.44853977024608	30.1621441564347	17.903728642639344
88-89	19.394531941589953	31.79939393886263	30.97154947633529	17.83452464321212
90-91	18.90033276417517	32.13644945441187	30.573856156857655	18.38936162455531
92-93	19.784088170635407	31.798708443466744	30.333266028038196	18.08393735785965
94-95	19.707541572788408	31.91335486069784	29.628357958994844	18.75074560751891
96-97	19.141305222404313	32.60442451205582	30.470491418190154	17.78377884734971
98-99	19.84899409654235	31.8266147747634	30.046867087985866	18.27752404070838
100-101	19.884621885843796	32.79122421238644	29.169153710251056	18.155000191518713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37957.0
1	43007.0
2	42344.5
3	41445.5
4	51691.0
5	63691.0
6	68944.5
7	69457.5
8	73890.5
9	78161.0
10	82568.5
11	86679.5
12	92018.0
13	96284.0
14	105771.5
15	113073.0
16	112636.5
17	118322.0
18	129079.0
19	142892.0
20	161957.5
21	187117.0
22	217951.0
23	253104.5
24	303283.5
25	371400.0
26	442351.0
27	525101.0
28	601813.5
29	682213.0
30	767621.5
31	807083.5
32	842205.5
33	907565.5
34	977338.0
35	1010361.5
36	1059344.0
37	1095726.5
38	1109727.0
39	1122907.0
40	1153089.0
41	1150622.5
42	990640.0
43	844664.5
44	773468.0
45	712150.5
46	673262.0
47	632599.0
48	583816.0
49	535936.0
50	470402.0
51	401535.5
52	351416.5
53	308274.5
54	272664.5
55	275430.0
56	249271.5
57	187548.0
58	156471.0
59	125363.0
60	90980.0
61	63954.5
62	47896.5
63	36759.0
64	26411.5
65	18022.0
66	11936.5
67	8010.0
68	5279.0
69	3390.0
70	2306.5
71	1646.0
72	1231.0
73	996.5
74	760.5
75	561.0
76	457.5
77	394.0
78	305.5
79	226.5
80	169.5
81	133.5
82	103.5
83	77.5
84	50.5
85	39.5
86	38.0
87	25.5
88	23.0
89	22.5
90	12.0
91	6.0
92	6.0
93	6.0
94	4.5
95	2.0
96	1.0
97	2.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12686690556759034
2	0.15225171613206046
3	0.14867048816708942
4	0.0748819528207504
5	0.07512578110772715
6	0.1439158365710428
7	0.1437863027935864
8	0.15094113908956042
9	0.17458486329233716
10-11	0.1585169601622677
12-13	0.1769698087243285
14-15	0.19213669013767917
16-17	0.2232267016357068
18-19	0.24383971642770227
20-21	0.2614144021749483
22-23	0.26407746424677253
24-25	0.26669290360629655
26-27	0.2402261050183671
28-29	0.22860044849165534
30-31	0.2305415502450093
32-33	0.28265032204382967
34-35	0.2862848874465768
36-37	0.2924720302286119
38-39	0.28667729859593005
40-41	0.2949674603531396
42-43	0.3026366219419552
44-45	0.2970361909754579
46-47	0.2946798191708467
48-49	0.06318771958832642
50-51	0.025567681779702665
52-53	0.01434967567028015
54-55	0.02877554768024054
56-57	0.03640089637374
58-59	0.03278728496440488
60-61	0.02833360891009518
62-63	0.03010326889916862
64-65	0.034284543039121484
66-67	0.043595735748046135
68-69	0.03918396768056056
70-71	0.03770385378227201
72-73	0.039532565934597634
74-75	0.030188989781308885
76-77	0.03423692032682134
78-79	0.027377344847108236
80-81	0.04124126885192689
82-83	0.05475278478572446
84-85	0.05982365119144407
86-87	0.04984955032730138
88-89	0.045767331428932814
90-91	0.049659059478100794
92-93	0.044984414038718404
94-95	0.04185464938635278
96-97	0.04469296303944151
98-99	0.035067460429335895
100-101	0.039374458529761146
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.624798E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.67190502178004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15487688029617	27.44450373204322
2	9.535679408167052	6.612403415162235
3	3.460877455126961	3.5998564324854536
4	1.8346349526072718	2.5444115530574902
5	1.171597083514984	2.031075140171301
6	0.7717381065461637	1.6054578199114173
7	0.5557003399661897	1.3487032585515026
8	0.43571381056842867	1.2085622285365136
9	0.3272108599936024	1.0210521473813832
>10	2.2979897652732126	15.510057096578539
>50	0.24655448858670043	5.945468571475744
>100	0.1764878386313375	12.019914222046435
>500	0.018216493780965026	4.337125983104838
>1k	0.011303282531700876	7.65121932767374
>5k	0.0010231689926509847	2.3970109003279987
>10k+	3.960654165096804E-4	4.7231781714920436
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	154689	0.5893367794397893	Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	141693	0.5398243979155729	No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	101864	0.38808319725937	Illumina Single End PCR Primer 1 (97% over 44bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	79088	0.3013108056315191	No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	72155	0.2748973444813658	Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	56209	0.21414600285431487	Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	54053	0.20593203743678562	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52634	0.20052590713647298	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	40564	0.1545414161394515	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	35818	0.13646002473333185	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	33187	0.12643639624839703	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31598	0.1203825970608024	No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	30412	0.1158641541177645	No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC	26797	0.10209166572056211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7044389701607514	0.0	0.0	6.476688872819928E-5	3.8098169840117222E-6
2	0.7067058112662384	3.8098169840117222E-6	0.0	7.619633968023443E-5	3.8098169840117222E-6
3	0.7069610690041672	3.8098169840117222E-6	0.0	9.905524158430477E-5	3.8098169840117222E-6
4	1.292876632792314	3.8098169840117222E-6	0.0	1.0667487555232822E-4	3.8098169840117222E-6
5	1.3012353712552356	3.8098169840117222E-6	0.0	1.1429450952035166E-4	3.8098169840117222E-6
6	1.3039936787516602	3.8098169840117222E-6	0.0	1.2953377745639856E-4	3.8098169840117222E-6
7	1.3340150365856724	3.8098169840117222E-6	0.0	1.7906139824855094E-4	3.8098169840117222E-6
8	1.66798359340414	3.8098169840117222E-6	0.0	1.904908492005861E-4	3.8098169840117222E-6
9	1.6700789927453465	3.8098169840117222E-6	0.0	2.4382828697675022E-4	3.8098169840117222E-6
10-11	1.7887395525293757	3.8098169840117222E-6	0.0	2.533528294367795E-4	3.8098169840117222E-6
12-13	1.8514148517333524	3.8098169840117222E-6	0.0	2.7049700586483224E-4	3.8098169840117222E-6
14-15	1.8751976342560455	3.8098169840117222E-6	0.0	3.124049926889612E-4	3.8098169840117222E-6
16-17	1.8919551142602211	3.8098169840117222E-6	0.0	3.5050316252907847E-4	3.8098169840117222E-6
18-19	1.9033578964933682	3.8098169840117222E-6	0.0	4.0574550879724836E-4	3.8098169840117222E-6
20-21	1.9098117264642842	3.8098169840117222E-6	0.0	4.990860249055356E-4	3.8098169840117222E-6
22-23	1.9135491569255998	3.8098169840117222E-6	0.0	5.295645607776294E-4	5.714725476017583E-6
24-25	1.9171170505311266	3.8098169840117222E-6	0.0	6.552885212500162E-4	7.6196339680234444E-6
26-27	1.9208392417245062	3.8098169840117222E-6	0.0	9.524542460029304E-4	7.6196339680234444E-6
28-29	1.9248071661133543	3.8098169840117222E-6	0.0	0.001097227291395376	7.6196339680234444E-6
30-31	1.9293122746969482	7.6196339680234444E-6	0.0	0.001165803997107587	9.524542460029304E-6
32-33	1.933556410817137	7.6196339680234444E-6	0.0	0.0011829481735356397	1.5239267936046889E-5
34-35	1.9384463109161163	1.1429450952035166E-5	0.0	0.001230570885835786	1.5239267936046889E-5
36-37	1.9430790483686744	1.1429450952035166E-5	0.0	0.0013867733821802668	1.5239267936046889E-5
38-39	1.9480108564544776	1.1429450952035166E-5	0.0	0.0014267764605123898	1.5239267936046889E-5
40-41	1.953653195407799	1.1429450952035166E-5	0.0	0.0015144022511446595	1.5239267936046889E-5
42-43	1.9599813014182423	1.5239267936046889E-5	0.0	0.0016344114861410288	1.5239267936046889E-5
44-45	1.9708068963783116	1.5239267936046889E-5	0.0	0.0017410863616933569	1.5239267936046889E-5
46-47	2.053081799056537	1.5239267936046889E-5	0.0	0.0019696753807340603	1.5239267936046889E-5
48-49	2.069679266747384	1.5239267936046889E-5	0.0	0.0020268226354942364	1.5239267936046889E-5
50-51	2.0788685453128206	1.904908492005861E-5	0.0	0.0020477766289063006	1.5239267936046889E-5
52-53	2.0896941402728895	1.904908492005861E-5	0.0	0.002070635530810371	1.5239267936046889E-5
54-55	2.1019579411444234	1.904908492005861E-5	0.0	0.002099209158190459	1.5239267936046889E-5
56-57	2.114777975295623	1.904908492005861E-5	0.0	0.0021334975110465643	1.5239267936046889E-5
58-59	2.1309239796738644	1.904908492005861E-5	0.0	0.0021887398573147343	1.5239267936046889E-5
60-61	2.1504207180895447	1.904908492005861E-5	0.0	0.0022439822035829043	1.5239267936046889E-5
62-63	2.1673591644004606	1.904908492005861E-5	0.0	0.002346847262151221	1.5239267936046889E-5
64-65	2.1861491817656065	1.904908492005861E-5	0.0	0.0024459025037355253	1.5239267936046889E-5
66-67	2.2059507055400074	1.904908492005861E-5	0.0	0.0025087644839717187	1.5239267936046889E-5
68-69	2.2280362145963233	1.904908492005861E-5	0.0	0.002533528294367795	1.5239267936046889E-5
70-71	2.254958286313842	2.095399341206447E-5	0.0	0.002565911738731895	1.5239267936046889E-5
72-73	2.283573821680754	2.285890190407033E-5	0.0	0.002590675549127971	1.5239267936046889E-5
74-75	2.316431588259363	2.285890190407033E-5	0.0	0.00271068478412434	1.5239267936046889E-5
76-77	2.3556022215804795	2.285890190407033E-5	0.0	0.003002135783401237	1.5239267936046889E-5
78-79	2.397971196259674	2.285890190407033E-5	0.0	0.0031050008419695532	1.5239267936046889E-5
80-81	2.4464396879302712	2.285890190407033E-5	0.0	0.0032821573317260984	1.5239267936046889E-5
82-83	2.5036440899452073	2.6668718888082055E-5	0.0	0.0033907371157704327	1.5239267936046889E-5
84-85	2.571468356803076	3.0478535872093778E-5	0.0	0.0034059763837064796	1.5239267936046889E-5
86-87	2.654989069635073	3.42883528561055E-5	0.0	0.0034231205601345325	1.5239267936046889E-5
88-89	2.7536633295209763	3.42883528561055E-5	0.0	0.003453599096006626	1.7144176428052747E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	39090	0.0	73.34356	1
GCGTGTT	51675	0.0	55.362766	2
ACCACCG	17535	0.0	51.438076	1
CCACCGA	18200	0.0	49.66391	2
ACCGAGA	19115	0.0	47.47243	4
CCGAGAT	37835	0.0	46.68669	1
CACCGAG	19445	0.0	46.55199	3
CGAGATC	39950	0.0	44.215805	2
CGTGTTT	67955	0.0	42.709923	3
CGCGCGT	820	0.0	41.14706	1
CGGAGAT	17165	0.0	39.465908	42-43
CGGCGAC	3275	0.0	39.32351	1
CTCGCGT	270	0.0	38.721592	1
CTCGGAG	18545	0.0	37.13311	40-41
CAAAACG	44620	0.0	36.61318	9
TCTCGGA	19110	0.0	36.570484	40-41
TCGGAGA	19010	0.0	36.4614	42-43
GGCGACC	3745	0.0	33.88139	2
GTTTTTT	358690	0.0	33.726032	1
GAGCGGC	4685	0.0	31.746506	9
>>END_MODULE