FastQCFastQC Report
Fri 27 May 2016
SRR1233051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26247980
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1640820.6251223903706113TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1356560.5168245327830941TruSeq Adapter, Index 10 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1079310.41119735690136916No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT778430.2965675834864245No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC771410.29389309196364827TruSeq Adapter, Index 10 (100% over 42bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA533820.20337565024051374No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440720.1679062541193646No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT425450.16208866358477872No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC371360.14148136351825932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318090.12118646844442886No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA311960.11885105063322969No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC288730.11000084577937046No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA265280.10106682495186296No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT264560.10079251812901412TruSeq Adapter, Index 10 (100% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT161800.084.488641
CGGAGAT129500.077.260111
CGCGTGT413550.075.4461441
GTGGTCG65000.072.996363
GTCGCCG31950.064.5160756
CCGAGAT138500.062.2731481
GGTAGAT142350.062.2591635
GTAGATC152200.058.2611166
CGCCGTA13000.057.35958
GCGTGTT544000.057.133852
GGTCGCC37600.054.9478235
GGTGGTA168100.051.704992
GTGGTAG169750.051.2863433
TAGATCG174450.050.721377
GGTCGAG31550.050.280115
CGGTCGA5400.050.1337474
TCGCCGT11900.049.8898477
GGTGGTC102800.048.419162
CGAGATC178400.047.867852
TGGTAGA184700.047.26374
Produced by FastQC (version 0.11.5)