##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233051_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26247980
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.798926279279396	34.0	31.0	34.0	30.0	34.0
2	32.03340119887321	34.0	31.0	34.0	30.0	34.0
3	32.1388482847061	34.0	31.0	34.0	30.0	34.0
4	35.38032610509456	37.0	37.0	37.0	33.0	37.0
5	35.24561558641846	37.0	35.0	37.0	33.0	37.0
6	35.20611536583006	37.0	36.0	37.0	33.0	37.0
7	35.1955964611372	37.0	36.0	37.0	33.0	37.0
8	35.18153648395038	37.0	36.0	37.0	33.0	37.0
9	36.72630377651918	39.0	38.0	39.0	34.0	39.0
10-11	36.65370194201611	39.0	38.0	39.0	33.0	39.0
12-13	36.67055720097318	39.0	38.0	39.0	33.0	39.0
14-15	38.1264708750921	41.0	39.0	41.0	33.5	41.0
16-17	37.94937046584157	41.0	39.0	41.0	33.0	41.0
18-19	37.6730579457924	41.0	38.5	41.0	33.0	41.0
20-21	37.35208016007327	41.0	38.5	41.0	32.0	41.0
22-23	37.056122166353376	41.0	38.0	41.0	32.0	41.0
24-25	36.821635379179654	41.0	38.0	41.0	30.5	41.0
26-27	36.387485817956275	40.0	38.0	41.0	28.0	41.0
28-29	35.59349363265287	40.0	36.5	41.0	20.5	41.0
30-31	35.394882006158184	40.0	37.0	41.0	17.5	41.0
32-33	35.23816651795681	40.0	37.0	41.0	14.5	41.0
34-35	35.01577767127223	40.0	37.0	41.0	9.0	41.0
36-37	34.86070027103038	40.0	37.0	41.0	7.5	41.0
38-39	34.72849455462858	40.0	36.5	41.0	4.5	41.0
40-41	34.56873713710541	40.0	36.0	41.0	2.0	41.0
42-43	34.34853689693455	40.0	35.5	41.0	2.0	41.0
44-45	34.228643499423576	40.0	35.0	41.0	2.0	41.0
46-47	34.03368573505466	40.0	35.0	41.0	2.0	41.0
48-49	33.85635003150719	40.0	35.0	41.0	2.0	41.0
50-51	33.61580849269163	39.0	35.0	40.5	2.0	41.0
52-53	33.355187656345365	39.0	35.0	40.0	2.0	41.0
54-55	33.11265823122389	38.5	34.0	40.0	2.0	41.0
56-57	32.86516514794663	38.0	34.0	40.0	2.0	41.0
58-59	32.52070778398947	38.0	33.5	40.0	2.0	41.0
60-61	32.14644387872896	38.0	33.0	40.0	2.0	41.0
62-63	31.809900552347266	37.5	32.5	40.0	2.0	41.0
64-65	31.343359831880395	36.5	31.5	39.0	2.0	40.0
66-67	30.86724146010474	36.0	31.0	39.0	2.0	40.0
68-69	30.41519351203407	35.5	30.5	38.5	2.0	40.0
70-71	29.77287974160297	35.0	29.5	38.0	2.0	40.0
72-73	29.196432201639897	34.5	29.0	37.5	2.0	39.0
74-75	28.51357222536744	34.0	28.0	36.5	2.0	39.0
76-77	27.475699349054672	32.5	26.0	36.0	2.0	38.0
78-79	27.504488364437947	33.0	26.0	35.5	2.0	38.0
80-81	27.156133176724456	33.0	26.0	35.0	2.0	37.0
82-83	26.657533855938627	32.5	25.5	35.0	2.0	36.5
84-85	26.122399076043187	32.0	25.0	34.5	2.0	36.0
86-87	25.472874922184488	31.0	23.0	34.0	2.0	35.5
88-89	24.832895350423158	31.0	20.0	34.0	2.0	35.0
90-91	24.150801852180624	30.5	17.5	34.0	2.0	35.0
92-93	23.425390639584457	29.5	9.0	34.0	2.0	35.0
94-95	22.54945978700075	29.0	2.0	33.0	2.0	34.0
96-97	21.391913739647777	29.0	2.0	32.0	2.0	34.0
98-99	19.984042733955146	28.0	2.0	32.0	2.0	34.0
100-101	14.311957091555236	10.0	2.0	27.0	2.0	30.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.25478072812433084
1101	2	0.2944978289189635
1101	3	0.3976801133175414
1101	4	0.27072573544521106
1101	5	0.25274454020745907
1101	6	0.25560010877079975
1101	7	0.2218742026746625
1101	8	0.16418528821009915
1101	9	0.24677404391894697
1101	10-11	0.28985056550143184
1101	12-13	0.30580769093372595
1101	14-15	0.327442845379764
1101	16-17	0.32166829191228175
1101	18-19	0.3208977630790315
1101	20-21	0.33994174022519985
1101	22-23	0.3728071691002768
1101	24-25	0.3635633200030739
1101	26-27	0.33935705678814543
1101	28-29	0.725834451609451
1101	30-31	0.5989791926001757
1101	32-33	0.44169605317808447
1101	34-35	0.4528901753706691
1101	36-37	0.38333727043195864
1101	38-39	0.42684733377853235
1101	40-41	0.43244225493763366
1101	42-43	0.476129312691306
1101	44-45	0.47909853755395915
1101	46-47	0.5053847187915395
1101	48-49	0.44451301833129264
1101	50-51	0.5298564534051948
1101	52-53	0.5331861147803707
1101	54-55	0.5817335157542161
1101	56-57	0.5946395616937892
1101	58-59	0.5314516325424279
1101	60-61	0.621634105236204
1101	62-63	0.6264621887822486
1101	64-65	0.5925551748471598
1101	66-67	0.631231599175873
1101	68-69	0.7131452648099952
1101	70-71	0.6805805510628069
1101	72-73	0.6373054249885861
1101	74-75	0.643621834405991
1101	76-77	0.7021144587637487
1101	78-79	0.7283956880187894
1101	80-81	0.8014491824451646
1101	82-83	0.7917655682276674
1101	84-85	0.8529569272581945
1101	86-87	0.8844574867606312
1101	88-89	0.7297260155734904
1101	90-91	0.7101167159430055
1101	92-93	0.7908363569618047
1101	94-95	0.8613136679271953
1101	96-97	0.8785521773803708
1101	98-99	0.8637320397689869
1101	100-101	0.5502554482674391
1102	1	0.01319447493082393
1102	2	0.02388438266531523
1102	3	0.09891258055925789
1102	4	-0.020477304682714248
1102	5	0.05247320402109068
1102	6	0.08606708544091646
1102	7	0.0549053970124902
1102	8	0.05775840055097348
1102	9	0.09170212169091485
1102	10-11	0.1119207354168239
1102	12-13	0.12873977509342183
1102	14-15	0.09821299275844098
1102	16-17	0.06792844740621717
1102	18-19	0.07120820430741759
1102	20-21	0.12088835314471424
1102	22-23	0.09056570916133211
1102	24-25	0.09519832677980844
1102	26-27	0.11728165242200816
1102	28-29	0.5007085654628725
1102	30-31	0.34458970620949714
1102	32-33	0.1997037720131729
1102	34-35	0.1802497551406006
1102	36-37	0.14871082694265425
1102	38-39	0.1784495411715099
1102	40-41	0.16721720413267604
1102	42-43	0.17248447611547846
1102	44-45	0.16872239478319528
1102	46-47	0.24243889316368694
1102	48-49	0.19095551101039376
1102	50-51	0.20333032609497792
1102	52-53	0.24337076128610846
1102	54-55	0.24310039788399962
1102	56-57	0.2798423348387544
1102	58-59	0.2516420652310032
1102	60-61	0.36275294080543574
1102	62-63	0.2480634051363637
1102	64-65	0.22528202626895322
1102	66-67	0.2707710233397158
1102	68-69	0.3596365620558899
1102	70-71	0.320691504222296
1102	72-73	0.20485692172031378
1102	74-75	0.11291234102678871
1102	76-77	0.2842258913239135
1102	78-79	0.3957326889143786
1102	80-81	0.47179631190119764
1102	82-83	0.5158067623076761
1102	84-85	0.5613540795894458
1102	86-87	0.5229999853577425
1102	88-89	0.3641038353262118
1102	90-91	0.3418555317714329
1102	92-93	0.37106633870747885
1102	94-95	0.4019521477166492
1102	96-97	0.43795657814813893
1102	98-99	0.4583795865107696
1102	100-101	-0.09700874976718588
1103	1	0.04417256324154195
1103	2	0.08802230453922988
1103	3	0.20429160247183376
1103	4	0.060576577791387365
1103	5	0.050120673574475916
1103	6	0.06399436520451474
1103	7	0.044285787235772034
1103	8	0.029402679153463396
1103	9	0.0704506912592251
1103	10-11	0.13934576317697633
1103	12-13	0.16918647095778994
1103	14-15	0.2020202701269369
1103	16-17	0.23232288411752222
1103	18-19	0.22370302667680164
1103	20-21	0.259775810239951
1103	22-23	0.2728798558793528
1103	24-25	0.2879295819702037
1103	26-27	0.2850178331529776
1103	28-29	0.6437040962071094
1103	30-31	0.47892610463139107
1103	32-33	0.37451993593161603
1103	34-35	0.3519085660350072
1103	36-37	0.3242754810357624
1103	38-39	0.35392201535462675
1103	40-41	0.3614031330694729
1103	42-43	0.39174796254779665
1103	44-45	0.41922377890644924
1103	46-47	0.4678878084574265
1103	48-49	0.4675365694293063
1103	50-51	0.45757019031145063
1103	52-53	0.4936019096450437
1103	54-55	0.5800788010847384
1103	56-57	0.5810754886882776
1103	58-59	0.5490502068579275
1103	60-61	0.6470517152318607
1103	62-63	0.6392726109645679
1103	64-65	0.6150086828311281
1103	66-67	0.5873519235758735
1103	68-69	0.7231603823016997
1103	70-71	0.743842389291764
1103	72-73	0.6641006598902877
1103	74-75	0.6555338441899607
1103	76-77	0.7276377866296819
1103	78-79	0.7852008399866826
1103	80-81	0.8448206623183516
1103	82-83	0.9767006580144617
1103	84-85	0.9951422949240118
1103	86-87	1.0485819089897
1103	88-89	0.985878368808649
1103	90-91	0.9641586224470231
1103	92-93	1.0635146506895623
1103	94-95	1.144464564490118
1103	96-97	1.2061700195095142
1103	98-99	1.2211502256692839
1103	100-101	0.40317120879935864
1104	1	0.12861137513664644
1104	2	0.14192155445882548
1104	3	0.226538495571603
1104	4	0.11995857697706214
1104	5	0.11670385545287587
1104	6	0.13576170486128802
1104	7	0.14221877451309695
1104	8	0.1227299398514674
1104	9	0.17312204486255922
1104	10-11	0.20081012467342418
1104	12-13	0.18589109226012823
1104	14-15	0.19927210244125604
1104	16-17	0.2287863283481144
1104	18-19	0.21553139784310815
1104	20-21	0.22046326226881519
1104	22-23	0.25578223351220686
1104	24-25	0.2768413304418118
1104	26-27	0.24867032590934457
1104	28-29	0.6376138695119025
1104	30-31	0.4973655056660746
1104	32-33	0.38282288875213766
1104	34-35	0.3531458864518129
1104	36-37	0.3870768743842419
1104	38-39	0.39626453653450255
1104	40-41	0.40366630803165293
1104	42-43	0.4430694332178078
1104	44-45	0.4358001587674565
1104	46-47	0.5038962622919811
1104	48-49	0.5185696669292597
1104	50-51	0.47862331947145975
1104	52-53	0.5004445665405655
1104	54-55	0.6109324905943438
1104	56-57	0.6450009548840541
1104	58-59	0.6169870665055299
1104	60-61	0.7103487369843791
1104	62-63	0.7377343411154342
1104	64-65	0.7244617435155227
1104	66-67	0.6950489569899467
1104	68-69	0.7725148779755493
1104	70-71	0.7969242171222248
1104	72-73	0.735562463806918
1104	74-75	0.711262382717571
1104	76-77	0.8091175483114661
1104	78-79	0.8248297427398725
1104	80-81	0.9114979204909055
1104	82-83	1.085236129859389
1104	84-85	1.1287914050773118
1104	86-87	1.1435504946535175
1104	88-89	1.0584769264093303
1104	90-91	1.1167207930314227
1104	92-93	1.1382680045219935
1104	94-95	1.372702857143441
1104	96-97	1.4396105682342295
1104	98-99	1.5090902400779669
1104	100-101	0.5750285544948621
1105	1	0.04687851307705415
1105	2	0.04501076297594153
1105	3	0.143466897114223
1105	4	0.07516928003314405
1105	5	0.04977954269209306
1105	6	0.06794034509812974
1105	7	0.05293654350143839
1105	8	0.07368949212463605
1105	9	0.04184598475459467
1105	10-11	0.056700047929396646
1105	12-13	0.05860351978277123
1105	14-15	0.03655281729384541
1105	16-17	0.055007429594176926
1105	18-19	0.017298775188187676
1105	20-21	0.06528789075368735
1105	22-23	0.03674211884626288
1105	24-25	0.05401977577955819
1105	26-27	0.06920885794634302
1105	28-29	0.43528558383884786
1105	30-31	0.22596497887057154
1105	32-33	0.11105133436876002
1105	34-35	0.12134565029009536
1105	36-37	0.10190006437232313
1105	38-39	0.12711057775877066
1105	40-41	0.1499895605461674
1105	42-43	0.1672830689583833
1105	44-45	0.16762259816214709
1105	46-47	0.1852616223495147
1105	48-49	0.19147892059179838
1105	50-51	0.23005143766099678
1105	52-53	0.2860212050146558
1105	54-55	0.31742600479008587
1105	56-57	0.34589081881859585
1105	58-59	0.32814386282349517
1105	60-61	0.4398214571152579
1105	62-63	0.44325500481546953
1105	64-65	0.4350437913970815
1105	66-67	0.43668228123715025
1105	68-69	0.5061926397377619
1105	70-71	0.5633502377659028
1105	72-73	0.5552054472757
1105	74-75	0.585241209401957
1105	76-77	0.6397968083280006
1105	78-79	0.6343142382622489
1105	80-81	0.7157039344583858
1105	82-83	0.8326769894670747
1105	84-85	0.9131976340883945
1105	86-87	1.0142527308161107
1105	88-89	0.8246282755421639
1105	90-91	0.8914135077986423
1105	92-93	0.9220449237569852
1105	94-95	1.0660538082440176
1105	96-97	1.2656179633186255
1105	98-99	1.1324724527981829
1105	100-101	0.1048519424829788
1106	1	-0.07130207178419923
1106	2	-0.032469315010210664
1106	3	0.09597932101181073
1106	4	0.048387372513850835
1106	5	0.004616776301311631
1106	6	-0.0024055415580406247
1106	7	0.045012516890984955
1106	8	0.03035846552917576
1106	9	-0.029073973164962297
1106	10-11	-0.0036041665279427093
1106	12-13	-0.0023338257672023133
1106	14-15	-0.0702582529448037
1106	16-17	-0.0846786141307092
1106	18-19	-0.0660047501214649
1106	20-21	-0.02742687856858339
1106	22-23	0.009129924536082967
1106	24-25	-0.020818978927607645
1106	26-27	-0.019017022037260745
1106	28-29	0.32426267490823335
1106	30-31	0.1524068508091716
1106	32-33	0.04027038997913479
1106	34-35	0.0050963736417628525
1106	36-37	-0.01796956880463796
1106	38-39	0.019652938455969604
1106	40-41	0.00236061781142638
1106	42-43	0.01792837679811754
1106	44-45	0.003830603106941055
1106	46-47	0.022724840866366947
1106	48-49	0.0031179644205110435
1106	50-51	0.04458668162845214
1106	52-53	0.10310066717480737
1106	54-55	0.14026107981606373
1106	56-57	0.12665661007988405
1106	58-59	0.1370236658467121
1106	60-61	0.18379837386712694
1106	62-63	0.24946045455023125
1106	64-65	0.28546937431570996
1106	66-67	0.28547070318036916
1106	68-69	0.34382386189414404
1106	70-71	0.4879414851809436
1106	72-73	0.4130586917414476
1106	74-75	0.42679132575895906
1106	76-77	0.5319236033291652
1106	78-79	0.5416935201286357
1106	80-81	0.6533330016260592
1106	82-83	0.7355939058811458
1106	84-85	0.8093177845717783
1106	86-87	0.8311257232250675
1106	88-89	0.7319606979490558
1106	90-91	0.7476455764583108
1106	92-93	0.7723039517500858
1106	94-95	1.0717911841780783
1106	96-97	1.1244065371469887
1106	98-99	1.1022977921001669
1106	100-101	-0.16030212833002544
1107	1	-0.5091678833601918
1107	2	-0.21131717270022676
1107	3	-0.05888458948476938
1107	4	-0.020247754452093147
1107	5	-0.13978864550190195
1107	6	-0.14417707803885804
1107	7	-0.13104669861159834
1107	8	-0.1599446274929761
1107	9	-0.2244523463458563
1107	10-11	-0.2608301554717656
1107	12-13	-0.20585950628990446
1107	14-15	-0.2679678637599281
1107	16-17	-0.27041530299150907
1107	18-19	-0.3159928767916824
1107	20-21	-0.30286216951791545
1107	22-23	-0.2906300407093241
1107	24-25	-0.3096713605355035
1107	26-27	-0.3049965587237011
1107	28-29	-0.012580507850124434
1107	30-31	-0.10580045372309854
1107	32-33	-0.22415505403090208
1107	34-35	-0.26886141872945757
1107	36-37	-0.2791028354499332
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2308	28-29	-0.3325493115968854
2308	30-31	-0.543376095984982
2308	32-33	-0.7433808545624458
2308	34-35	-0.9296292043062664
2308	36-37	-0.9895268511197983
2308	38-39	-1.024873891306811
2308	40-41	-1.016227818324495
2308	42-43	-1.1525577904969424
2308	44-45	-1.3247369572958263
2308	46-47	-1.2195925723418028
2308	48-49	-1.1053133669571338
2308	50-51	-1.1133257351912889
2308	52-53	-1.3259833069677356
2308	54-55	-1.468871755441377
2308	56-57	-1.197790129177136
2308	58-59	-1.2474933420037289
2308	60-61	-1.3898517808248272
2308	62-63	-1.4907765307710363
2308	64-65	-1.4861385562371403
2308	66-67	-1.4922061572596768
2308	68-69	-1.5548920362301004
2308	70-71	-1.5630522506993607
2308	72-73	-1.7434578620921037
2308	74-75	-1.763849238624374
2308	76-77	-1.6791677636268325
2308	78-79	-1.7829451044102207
2308	80-81	-1.7431363167243177
2308	82-83	-1.9967220254470455
2308	84-85	-2.0095191579381613
2308	86-87	-2.06150916059498
2308	88-89	-2.179584455291671
2308	90-91	-2.170709773986921
2308	92-93	-2.3613004031805715
2308	94-95	-2.5475974364109284
2308	96-97	-2.701727063480085
2308	98-99	-2.8556950370759857
2308	100-101	-1.0409402543597057
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	469837.0
3	237886.0
4	109087.0
5	85515.0
6	158223.0
7	154423.0
8	128996.0
9	200751.0
10	342652.0
11	397191.0
12	306116.0
13	230743.0
14	173512.0
15	157146.0
16	147654.0
17	140681.0
18	135794.0
19	135196.0
20	137962.0
21	145531.0
22	158358.0
23	175273.0
24	197558.0
25	225953.0
26	272603.0
27	329396.0
28	327487.0
29	363325.0
30	428683.0
31	533450.0
32	725562.0
33	1065831.0
34	1730085.0
35	3145594.0
36	5310572.0
37	5374958.0
38	1845371.0
39	43025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.886452012511064	17.2365543977358	9.16493867861201	46.712054911141124
2	16.074881903131157	18.734095897187224	52.18747418845453	13.003548011227089
3	19.77346447231368	23.01495962736942	36.9743347869055	20.237241113411393
4	19.7899724093054	29.9328748345587	33.357500272401914	16.919652483733984
5	21.935432745681762	32.031459182763776	32.1694469441077	13.863661127446761
6	17.416707876187044	34.76705255032959	33.600677080674394	14.215562492808969
7	13.565036242788969	19.078340504678838	52.69890102019279	14.657722232339404
8	13.557763302166489	23.393236355711945	44.28280195275979	18.766198389361772
9	15.893112536659965	21.555498746951194	44.6732586660002	17.878130050388638
10-11	18.36609140970086	32.52508193011424	33.846071202431574	15.262755457753322
12-13	17.38410346243787	26.217173664411508	39.08623444546971	17.31248842768091
14-15	17.356617537806716	28.048124084215242	37.92229154395881	16.672966834019228
16-17	18.60020656827687	28.2178666701209	36.20939401813016	16.97253274347207
18-19	18.639891526890832	28.152008268826783	36.04306693315066	17.165033271131723
20-21	19.06316028890604	27.972226815168256	35.20801600732704	17.756596888598665
22-23	18.14423247522877	30.27357742940775	34.91438682935607	16.66780326600741
24-25	18.592456290006538	29.04453938129738	33.998700890184914	18.36430343851117
26-27	18.148876599265925	29.414636859674538	33.6979226591913	18.73856388186824
28-29	19.377455880304044	29.53209602372694	32.596871963931996	18.493576132037028
30-31	18.87800889820855	30.04369669589812	32.72820613243381	18.35008827345952
32-33	18.20968699305623	30.479151157536695	32.3029829343058	19.008178915101276
34-35	18.37665031747205	30.450455616013116	32.45784437507191	18.71504969144292
36-37	19.12887582206326	31.02749240132002	31.34003264251192	18.503599134104796
38-39	18.279507604013716	30.129088028869273	33.10268828306026	18.48871608405675
40-41	18.395393260124262	30.753888780721823	32.80509261352013	18.045625345633788
42-43	18.025741409434172	30.19377300653231	33.41749727026613	18.362988313767385
44-45	18.789354776209926	29.57774459706628	32.81667697957621	18.816223647147588
46-47	18.321850721531906	30.88021457702791	32.78652796286521	18.01140673857498
48-49	18.410616740793007	30.285330528292082	33.30285797230872	18.001194758606186
50-51	18.172344205478527	30.134674942681777	33.13025229733406	18.562728554505636
52-53	19.716647409938652	29.80059904825398	32.294569153140955	18.188184388666414
54-55	18.341395389558148	30.788381053490355	31.984892250044222	18.885331306907275
56-57	19.511867568048483	30.009340410936552	32.47221016776108	18.006581853253884
58-59	18.449524115543575	30.954597137437446	31.79127119637367	18.804607550645308
60-61	18.82561486456883	30.260773821656418	31.54271906246845	19.37089225130631
62-63	19.21776451915252	30.930062519107597	31.71576458472008	18.1364083770198
64-65	18.732909191413285	31.35404141344252	31.03280326578433	18.880246129359872
66-67	19.12653347353663	31.733920434630214	31.04716240722961	18.092383684603544
68-69	19.017852526818565	32.24186787075122	30.803648492944298	17.936631109485916
70-71	18.314536272341815	32.68085347618919	30.34932934783242	18.655280903636566
72-73	18.45425773464037	32.607511665453714	30.599484930370163	18.338745669535754
74-75	18.988308962798335	32.409841783442744	30.16098439931446	18.440864854444463
76-77	18.44487682367125	32.737535162825765	30.360033808889103	18.457554204613878
78-79	18.43254031148265	33.24520998729308	29.822787741371314	18.499461959852955
80-81	18.424184701146697	33.17800656468427	30.35362113637307	18.044187597795965
82-83	18.868521579760937	32.522857549544504	30.18355914532866	18.4250617253659
84-85	18.653614769765937	32.315875733480496	30.423503473451817	18.607006023301746
86-87	18.872002617119808	32.91893253456211	29.791804324420134	18.417260523897944
88-89	18.9989016182862	32.17080678799709	30.350724742341413	18.4795668513753
90-91	18.555896774811146	32.60349415529599	29.80901553557476	19.031593534318105
92-93	19.336480579060797	32.26178637097396	29.93313219074174	18.468600859223503
94-95	19.464225131349455	32.35184190296008	29.26499849422226	18.918934471468205
96-97	18.56384369128696	32.96798392959287	29.722313557720724	18.745858821399445
98-99	19.575109469144337	32.071888107294114	29.41275130766428	18.940251115897276
100-101	19.48288444703464	33.0768647070986	28.33583675106388	19.10441409480288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26258.0
1	30530.5
2	30556.5
3	29536.5
4	37278.0
5	47410.5
6	53929.0
7	57111.5
8	62445.0
9	67849.0
10	73587.5
11	79560.0
12	86010.0
13	91220.5
14	99210.0
15	106898.5
16	109820.0
17	115636.0
18	125414.0
19	138129.0
20	155999.0
21	179719.0
22	209337.5
23	242989.5
24	290470.5
25	356571.5
26	427114.0
27	508443.5
28	585544.5
29	666620.5
30	751050.5
31	793967.0
32	834265.5
33	900912.0
34	964847.0
35	1001151.5
36	1058123.0
37	1099043.0
38	1116449.5
39	1133858.0
40	1162335.0
41	1160868.0
42	1025526.5
43	895944.0
44	832844.0
45	781168.5
46	741822.0
47	693242.0
48	631057.5
49	568440.5
50	492795.0
51	417630.5
52	361567.5
53	312879.0
54	263845.0
55	222617.0
56	195715.5
57	172654.5
58	149386.0
59	120852.5
60	88203.0
61	62494.0
62	47290.0
63	36397.5
64	25764.0
65	17174.5
66	11370.5
67	7738.5
68	5142.5
69	3252.5
70	2141.5
71	1548.5
72	1215.5
73	956.5
74	659.0
75	428.0
76	326.0
77	246.0
78	177.0
79	125.0
80	81.0
81	56.0
82	55.0
83	44.5
84	29.0
85	22.0
86	17.0
87	15.5
88	14.5
89	11.0
90	7.5
91	6.0
92	6.5
93	7.0
94	7.5
95	8.5
96	5.5
97	3.0
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19555409597233767
2	1.37153411424422E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.466933455450667E-4
24-25	2.4763810396076197E-5
26-27	0.0
28-29	3.42883528561055E-5
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.3334359444041027E-5
42-43	0.0
44-45	5.08610567365565E-4
46-47	9.505493375109247E-4
48-49	0.0
50-51	5.714725476017583E-6
52-53	4.190798682412894E-5
54-55	4.8384675696948873E-4
56-57	7.829173902144089E-4
58-59	2.457331954687561E-4
60-61	0.0012572396047238683
62-63	9.315002525908661E-4
64-65	0.0012153316178997392
66-67	0.0014477304539244544
68-69	8.038713836264733E-4
70-71	6.514787042660045E-4
72-73	2.7621173134084986E-4
74-75	3.619326134811136E-4
76-77	0.0016953685578852164
78-79	0.0033069211421221747
80-81	0.0015677396889208235
82-83	0.004084123806860566
84-85	0.011071328155538065
86-87	0.00889211284068336
88-89	0.027059225128943258
90-91	0.025861037687471566
92-93	0.02563435357692287
94-95	0.028145022969386597
96-97	0.023641819294284744
98-99	0.01979961886590892
100-101	0.01089417166578152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.624798E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.650752330814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31293000412538	28.275656242082608
2	9.490055671799533	6.766552487219238
3	3.395052885422462	3.631085687046349
4	1.828146331497051	2.6069916835475024
5	1.139710378215247	2.0315766211305064
6	0.8130171520476842	1.7390803877013443
7	0.5674377660518951	1.416070828246654
8	0.4544224718397407	1.2960402397691917
9	0.3353540778252713	1.0760062654509546
>10	2.231122521579607	15.4671873057911
>50	0.2342688573613168	5.8273241789463395
>100	0.16977628080976762	11.796129300655618
>500	0.016925182061533428	4.1480182198742
>1k	0.010528333669775387	7.304419303573001
>5k	8.743012174594949E-4	2.083269073512115
>10k+	3.777844766795469E-4	4.534592175452986
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG	164082	0.6251223903706113	TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC	135656	0.5168245327830941	TruSeq Adapter, Index 10 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	107931	0.41119735690136916	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	77843	0.2965675834864245	No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC	77141	0.29389309196364827	TruSeq Adapter, Index 10 (100% over 42bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	53382	0.20337565024051374	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44072	0.1679062541193646	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	42545	0.16208866358477872	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	37136	0.14148136351825932	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31809	0.12118646844442886	No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	31196	0.11885105063322969	No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC	28873	0.11000084577937046	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	26528	0.10106682495186296	No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT	26456	0.10079251812901412	TruSeq Adapter, Index 10 (100% over 46bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7061114798167326	0.0	0.0	3.809816984011722E-5	0.0
2	0.7084735663468198	0.0	0.0	4.9527620792152386E-5	0.0
3	0.7086716768299884	0.0	0.0	5.714725476017583E-5	0.0
4	0.826200720969766	0.0	0.0	7.238652269622272E-5	0.0
5	0.8344642140080875	0.0	0.0	8.381597364825788E-5	0.0
6	0.8369596441326151	0.0	0.0	1.1429450952035166E-4	0.0
7	0.8620777675082044	3.8098169840117222E-6	0.0	1.714417642805275E-4	0.0
8	1.1990713190119773	3.8098169840117222E-6	0.0	2.0192030015262128E-4	0.0
9	1.2011971968890558	3.8098169840117222E-6	0.0	2.4382828697675022E-4	3.8098169840117222E-6
10-11	1.2969150387953663	3.8098169840117222E-6	0.0	2.495430124527678E-4	3.8098169840117222E-6
12-13	1.360927583760731	3.8098169840117222E-6	0.0	2.590675549127971E-4	3.8098169840117222E-6
14-15	1.3927528899366732	3.8098169840117222E-6	0.0	3.200246266569847E-4	3.8098169840117222E-6
16-17	1.4126972818479746	3.8098169840117222E-6	0.0	3.6002770498910774E-4	3.8098169840117222E-6
18-19	1.4199759371959289	3.8098169840117222E-6	0.0	4.628927635574242E-4	3.8098169840117222E-6
20-21	1.4251077606733928	3.8098169840117222E-6	0.0	6.571934297420221E-4	7.6196339680234444E-6
22-23	1.42881852241582	5.714725476017583E-6	0.0	6.743376061700748E-4	7.6196339680234444E-6
24-25	1.432329268766587	7.6196339680234444E-6	0.0	7.657732137863561E-4	7.6196339680234444E-6
26-27	1.4359924077967143	7.6196339680234444E-6	0.0	0.001097227291395376	7.6196339680234444E-6
28-29	1.4397069793561257	7.6196339680234444E-6	0.0	0.0012648592386918917	9.524542460029304E-6
30-31	1.4439511154763147	7.6196339680234444E-6	0.0	0.0013105770425000324	1.1429450952035166E-5
32-33	1.4477837913622307	7.6196339680234444E-6	0.0	0.0013258163104360792	1.1429450952035166E-5
34-35	1.452500344788437	7.6196339680234444E-6	0.0	0.0013543899378161673	1.1429450952035166E-5
36-37	1.4568378214247344	7.6196339680234444E-6	0.0	0.0015563102379687883	1.1429450952035166E-5
38-39	1.4613924576291204	7.6196339680234444E-6	0.0	0.0015867887738408822	1.1429450952035166E-5
40-41	1.4663661737017477	7.6196339680234444E-6	0.0	0.0016839391069331812	1.1429450952035166E-5
42-43	1.4720561353673691	7.6196339680234444E-6	0.0	0.0017925188909775152	1.1429450952035166E-5
44-45	1.4779613516925874	7.6196339680234444E-6	0.0	0.00189157413256182	1.1429450952035166E-5
46-47	1.4846113872381799	7.6196339680234444E-6	0.0	0.0021201631516025235	1.1429450952035166E-5
48-49	1.491787177527566	1.1429450952035166E-5	1.9049084920058611E-6	0.002183025131838717	1.1429450952035166E-5
50-51	1.500256400683024	1.1429450952035166E-5	3.8098169840117222E-6	0.002203979125250781	1.1429450952035166E-5
52-53	1.5095333050390924	1.3334359444041027E-5	3.8098169840117222E-6	0.002221123301678834	1.1429450952035166E-5
54-55	1.5190140346038057	1.5239267936046889E-5	3.8098169840117222E-6	0.0022496969290589216	1.1429450952035166E-5
56-57	1.529529129479678	1.5239267936046889E-5	3.8098169840117222E-6	0.002295414732867062	1.1429450952035166E-5
58-59	1.5416748260247075	1.5239267936046889E-5	3.8098169840117222E-6	0.0023563718046112504	1.1429450952035166E-5
60-61	1.5548682222403398	1.5239267936046889E-5	3.8098169840117222E-6	0.0023925650659593614	1.1429450952035166E-5
62-63	1.5699760514904386	1.5239267936046889E-5	3.8098169840117222E-6	0.0024763810396076194	1.1429450952035166E-5
64-65	1.5862534945546285	1.5239267936046889E-5	3.8098169840117222E-6	0.0025982951830959946	1.1429450952035166E-5
66-67	1.603963428804807	1.904908492005861E-5	3.8098169840117222E-6	0.0026897307907122756	1.1429450952035166E-5
68-69	1.6238125752915082	1.904908492005861E-5	3.8098169840117222E-6	0.0027259240520603875	1.1429450952035166E-5
70-71	1.647505827115077	1.904908492005861E-5	3.8098169840117222E-6	0.0027468780454724517	1.1429450952035166E-5
72-73	1.6738659508274543	1.904908492005861E-5	3.8098169840117222E-6	0.0027773565813445453	1.1429450952035166E-5
74-75	1.7032339250487085	1.904908492005861E-5	3.8098169840117222E-6	0.002916414901260973	1.1429450952035166E-5
76-77	1.7377299129304427	1.904908492005861E-5	3.8098169840117222E-6	0.0032954916911701396	1.1429450952035166E-5
78-79	1.7776263163870134	1.904908492005861E-5	3.8098169840117222E-6	0.00339645184124645	1.1429450952035166E-5
80-81	1.8238584454879958	1.904908492005861E-5	3.8098169840117222E-6	0.0035564641545749428	1.1429450952035166E-5
82-83	1.8789579236192653	1.904908492005861E-5	3.8098169840117222E-6	0.003674568481079306	1.1429450952035166E-5
84-85	1.9446258340641833	1.904908492005861E-5	3.8098169840117222E-6	0.003699332291475382	1.1429450952035166E-5
86-87	2.0251215522108748	1.904908492005861E-5	3.8098169840117222E-6	0.0037221911933794523	1.1429450952035166E-5
88-89	2.120109814164747	1.904908492005861E-5	3.8098169840117222E-6	0.0037717188141716046	1.1429450952035166E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	16180	0.0	84.48864	1
CGGAGAT	12950	0.0	77.26011	1
CGCGTGT	41355	0.0	75.446144	1
GTGGTCG	6500	0.0	72.99636	3
GTCGCCG	3195	0.0	64.516075	6
CCGAGAT	13850	0.0	62.273148	1
GGTAGAT	14235	0.0	62.259163	5
GTAGATC	15220	0.0	58.261116	6
CGCCGTA	1300	0.0	57.3595	8
GCGTGTT	54400	0.0	57.13385	2
GGTCGCC	3760	0.0	54.947823	5
GGTGGTA	16810	0.0	51.70499	2
GTGGTAG	16975	0.0	51.286343	3
TAGATCG	17445	0.0	50.72137	7
GGTCGAG	3155	0.0	50.28011	5
CGGTCGA	540	0.0	50.133747	4
TCGCCGT	1190	0.0	49.889847	7
GGTGGTC	10280	0.0	48.41916	2
CGAGATC	17840	0.0	47.86785	2
TGGTAGA	18470	0.0	47.2637	4
>>END_MODULE