FastQCFastQC Report
Fri 27 May 2016
SRR1233050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41815286
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4413981.0555900538382064Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2693950.6442500476978682Illumina Single End PCR Primer 1 (100% over 50bp)
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT2435800.582514250889017No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT1916590.4583467395153055No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT1082700.25892445169453104No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1065160.25472981339886086No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA900030.21523947008278266No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG774840.1853006577546785No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG707220.16912953793978594No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704460.16846949223305563No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT679800.16257212733161744No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT675740.16160119053113736No Hit
ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT667680.15967366574988867No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT643320.15384804494700816No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA593910.14203179191456444No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT579540.13859524959365338No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT578520.1383513196585574No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA561330.13424038281120448No Hit
AGATAATACGACTCACTATAGATAATACGACTCACTATAGATAATACGAC549830.1314901923664949No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT531950.12721424409245938No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT513330.12276132704198171No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT493290.11796882125833122No Hit
GTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATA474160.11339393924030557No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA466570.1115788135467972No Hit
GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA465650.11135879831122046No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT448290.10720720647468487No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT429990.1028308164626687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT403900.060.5727231
GAGCGGC86650.059.395019
ATCGGGA71500.053.6394232
AGAGCGG108950.048.4099128
GCGTGTT505350.048.0181242
GATCGGG84850.045.703641
CGCCGTA1032900.042.6800346-47
GTCGCCG1052200.042.3426544-45
CCGTATC1044100.042.27456748-49
AGATCGG1230900.042.087781
TCGGTGG1103750.040.74804338-39
TCTCGGT1082250.040.46638536-37
GTTTTTT5927500.039.951381
GATCTCG1198750.039.29548634-35
TGGTCGC1086550.039.2734542-43
CCACCGA49150.039.2571832
GGTGGTC1090050.039.0500540-41
GGCGCCG84350.037.98375744-45
CGCCGGA54350.037.5910146-47
GTATCAT1189300.036.98683550-51
Produced by FastQC (version 0.11.5)