##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233050_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41815286
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.144028262774526	34.0	31.0	34.0	30.0	34.0
2	32.34740408089042	34.0	31.0	34.0	31.0	34.0
3	32.364402362332285	34.0	31.0	34.0	31.0	34.0
4	35.637863961997056	37.0	37.0	37.0	35.0	37.0
5	35.58915352151364	37.0	37.0	37.0	35.0	37.0
6	35.60024018489315	37.0	37.0	37.0	35.0	37.0
7	35.57995444536718	37.0	37.0	37.0	35.0	37.0
8	35.59914548952266	37.0	37.0	37.0	35.0	37.0
9	37.28553591621973	39.0	39.0	39.0	35.0	39.0
10-11	37.22133016141514	39.0	39.0	39.0	35.0	39.0
12-13	37.1711805941014	39.0	38.5	39.0	35.0	39.0
14-15	38.57362182097714	41.0	39.0	41.0	35.0	41.0
16-17	38.33801720260863	41.0	39.0	41.0	34.0	41.0
18-19	37.93272643166903	41.0	38.0	41.0	33.0	41.0
20-21	37.65452324061589	41.0	38.0	41.0	33.0	41.0
22-23	37.405779133018484	40.0	38.0	41.0	32.0	41.0
24-25	37.00926688627695	40.5	38.0	41.0	31.0	41.0
26-27	36.64536455639691	40.0	38.0	41.0	29.0	41.0
28-29	36.379994208338076	41.0	38.0	41.0	26.5	41.0
30-31	36.08968707041726	41.0	38.0	41.0	24.5	41.0
32-33	35.94575828083539	40.0	38.0	41.0	24.0	41.0
34-35	35.82278198455943	40.0	38.0	41.0	23.0	41.0
36-37	35.716756188155685	40.0	38.0	41.0	21.0	41.0
38-39	35.618993757450326	40.0	38.0	41.0	19.5	41.0
40-41	35.47114532470255	40.0	37.0	41.0	18.0	41.0
42-43	35.27543642772167	40.0	37.0	41.0	15.5	41.0
44-45	35.22240991009843	40.0	37.0	41.0	15.0	41.0
46-47	35.226773230727154	40.0	37.0	41.0	13.0	41.0
48-49	35.19333775452355	40.0	37.0	41.0	12.0	41.0
50-51	34.66675450934379	39.5	36.0	40.5	9.0	41.0
52-53	34.6956878520453	40.0	36.0	41.0	9.0	41.0
54-55	34.86250035453542	40.0	36.0	41.0	7.5	41.0
56-57	34.76611054388101	40.0	36.0	41.0	6.0	41.0
58-59	34.58711479337963	40.0	35.5	41.0	2.0	41.0
60-61	34.44454696543269	40.0	35.0	41.0	2.0	41.0
62-63	34.19583735478935	40.0	35.0	41.0	2.0	41.0
64-65	33.787067222259346	39.0	35.0	41.0	2.0	41.0
66-67	33.147111991533436	39.0	34.5	41.0	2.0	41.0
68-69	32.77651062819467	39.0	34.0	41.0	2.0	41.0
70-71	32.397822246151804	37.5	34.0	40.0	2.0	41.0
72-73	31.990717939846206	37.0	34.0	40.0	2.0	41.0
74-75	31.509896799462283	36.5	33.0	39.0	2.0	41.0
76-77	31.053409296303748	36.0	32.0	39.0	2.0	41.0
78-79	30.560218576527255	35.5	32.0	38.5	2.0	40.0
80-81	30.10611176974851	35.0	31.0	37.0	2.0	39.0
82-83	29.628461921795775	35.0	31.0	37.0	2.0	39.0
84-85	29.15885432422966	35.0	30.0	36.0	2.0	38.5
86-87	28.64845750427248	35.0	29.5	36.0	2.0	37.0
88-89	28.148756366272373	35.0	29.0	35.5	2.0	37.0
90-91	27.649722819066692	34.0	27.5	35.0	2.0	36.5
92-93	27.09587540546775	34.0	26.0	35.0	2.0	36.0
94-95	26.466386718005467	34.0	24.5	35.0	2.0	36.0
96-97	25.767939324867946	34.0	20.5	35.0	2.0	35.0
98-99	24.91610815480253	33.0	4.5	35.0	2.0	35.0
100-101	23.17504546064805	31.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.325694292416685
1101	2	0.32428112715739843
1101	3	0.3104171067132526
1101	4	0.30149824536199077
1101	5	0.26902783712170475
1101	6	0.21998958894916143
1101	7	0.21079150297820348
1101	8	0.1862450695692459
1101	9	0.29000059907910725
1101	10-11	0.304858564807553
1101	12-13	0.3054878863255013
1101	14-15	0.4070660071856693
1101	16-17	0.4125586437060633
1101	18-19	0.41419056440121693
1101	20-21	0.40749624119979444
1101	22-23	0.3323092892815538
1101	24-25	0.36591674945425723
1101	26-27	0.3659843204974891
1101	28-29	0.40453783256578646
1101	30-31	0.3960655506953614
1101	32-33	0.38764173349510855
1101	34-35	0.38042558256530157
1101	36-37	0.3704284767696038
1101	38-39	0.3143782617257429
1101	40-41	0.28686041053510536
1101	42-43	0.35594532247864663
1101	44-45	0.3440326099142865
1101	46-47	0.3940457212588271
1101	48-49	0.3886281276396275
1101	50-51	0.3104538136060597
1101	52-53	0.3305715498390569
1101	54-55	0.35510060632760343
1101	56-57	0.32753828964921183
1101	58-59	0.3420041592966001
1101	60-61	0.32451451821074784
1101	62-63	0.3446882478039015
1101	64-65	0.3382291879480377
1101	66-67	0.4577257040153313
1101	68-69	0.4574008582293345
1101	70-71	0.46283916880348386
1101	72-73	0.43482473955941003
1101	74-75	0.4846822633688497
1101	76-77	0.5321428234861401
1101	78-79	0.47906271595210015
1101	80-81	0.48108613960365076
1101	82-83	0.44277474983817555
1101	84-85	0.4634201370786428
1101	86-87	0.4470010125211168
1101	88-89	0.4589263398749388
1101	90-91	0.5098907803611255
1101	92-93	0.5198097274466953
1101	94-95	0.5283115920077961
1101	96-97	0.5494958931208593
1101	98-99	0.4938322854993231
1101	100-101	0.40631620514929523
1102	1	-0.054886642118020745
1102	2	0.05081292711687979
1102	3	0.04102202331004179
1102	4	0.02741847822048271
1102	5	0.024408240515512603
1102	6	-0.02818189744498767
1102	7	-0.004239351991259355
1102	8	-0.006392703704896974
1102	9	0.006086629894184625
1102	10-11	-0.02333318702648768
1102	12-13	0.023696983481947598
1102	14-15	0.0294610739025174
1102	16-17	-0.0013186393128066243
1102	18-19	0.014694701815550104
1102	20-21	0.036704035649279376
1102	22-23	-0.017745522932486324
1102	24-25	-0.06857010727303958
1102	26-27	-0.1460888885981788
1102	28-29	-0.13035489659066002
1102	30-31	-0.10693584744080198
1102	32-33	-0.05238183445312217
1102	34-35	-0.09925400194544665
1102	36-37	-0.12558640109067198
1102	38-39	-0.14023853570402167
1102	40-41	-0.18928727409650037
1102	42-43	-0.14813554340410207
1102	44-45	-0.13594828288407257
1102	46-47	-0.11986573226747055
1102	48-49	0.07963668475280627
1102	50-51	0.07894073794208367
1102	52-53	-0.09705967390305403
1102	54-55	-0.09116262266945085
1102	56-57	-0.1409462895873972
1102	58-59	-0.20167058220594214
1102	60-61	-0.19942320579292527
1102	62-63	-0.1633085070020428
1102	64-65	-0.13115519323731917
1102	66-67	-0.06064047121521554
1102	68-69	-0.03964470707538226
1102	70-71	-0.07863215762882447
1102	72-73	-0.092167584295332
1102	74-75	-0.03173215916239869
1102	76-77	-0.022117077810001717
1102	78-79	-0.06531731701445764
1102	80-81	-0.023631364840955627
1102	82-83	-0.027516771696536324
1102	84-85	0.08124018279275447
1102	86-87	0.06117586622707094
1102	88-89	0.05799337200885191
1102	90-91	0.0515581029083485
1102	92-93	0.06704145961194641
1102	94-95	0.08202094875229449
1102	96-97	0.19134175437206125
1102	98-99	0.2534652226673728
1102	100-101	0.21379900327519863
1103	1	0.21447282621474528
1103	2	0.2568529141765552
1103	3	0.2550850009596317
1103	4	0.21128705558376737
1103	5	0.20508900372374228
1103	6	0.20602348932283832
1103	7	0.1661979885988245
1103	8	0.15815724838353873
1103	9	0.18720580996226488
1103	10-11	0.19864213271532805
1103	12-13	0.23133749924974012
1103	14-15	0.24545642148182623
1103	16-17	0.27738873372467765
1103	18-19	0.2970248178695485
1103	20-21	0.32659973548114607
1103	22-23	0.2856889598979393
1103	24-25	0.30880899144260354
1103	26-27	0.34666675745909714
1103	28-29	0.31899995149662175
1103	30-31	0.36463106445773974
1103	32-33	0.3553872880257529
1103	34-35	0.35744091737759476
1103	36-37	0.36598822234975614
1103	38-39	0.3238564352561397
1103	40-41	0.3798455362759299
1103	42-43	0.3998678719021811
1103	44-45	0.418280281315603
1103	46-47	0.39211202444764126
1103	48-49	0.40678455010719006
1103	50-51	0.3937759642390901
1103	52-53	0.43514310394545674
1103	54-55	0.4487545006383229
1103	56-57	0.4668251895835809
1103	58-59	0.48909601806357017
1103	60-61	0.4554240347730598
1103	62-63	0.4697178751547568
1103	64-65	0.5230390280156456
1103	66-67	0.5136323489339603
1103	68-69	0.5191553717695285
1103	70-71	0.5025554237421304
1103	72-73	0.5358270892982588
1103	74-75	0.587353729046793
1103	76-77	0.6143040856175475
1103	78-79	0.5951897862092217
1103	80-81	0.577966985142151
1103	82-83	0.596285824089275
1103	84-85	0.6602727495493887
1103	86-87	0.6448114917097776
1103	88-89	0.7097565485362871
1103	90-91	0.814133917677804
1103	92-93	0.915872731621068
1103	94-95	0.9620260500577551
1103	96-97	1.1065741742329251
1103	98-99	1.15518545816348
1103	100-101	1.190822383385708
1104	1	0.1462463029379748
1104	2	0.14243237158635935
1104	3	0.1390815026453751
1104	4	0.16444387425977425
1104	5	0.14248268746551673
1104	6	0.11318244119285481
1104	7	0.10513364734438824
1104	8	0.10846850813918252
1104	9	0.1514897385607057
1104	10-11	0.14788982429282527
1104	12-13	0.16473071762045066
1104	14-15	0.14794205535879712
1104	16-17	0.20704423901258906
1104	18-19	0.20718293853766312
1104	20-21	0.2112627567316352
1104	22-23	0.18455518877955512
1104	24-25	0.20041458652132604
1104	26-27	0.18906361403725924
1104	28-29	0.2062282650125411
1104	30-31	0.21176039874210772
1104	32-33	0.20709098969598472
1104	34-35	0.19984901321945614
1104	36-37	0.19580370727700824
1104	38-39	0.1844186798294345
1104	40-41	0.18715389331580923
1104	42-43	0.18611585669801656
1104	44-45	0.22809012146390728
1104	46-47	0.2607907230221116
1104	48-49	0.2957073488854647
1104	50-51	0.2509612147464182
1104	52-53	0.22496897101802205
1104	54-55	0.2521065795780686
1104	56-57	0.2675401227129939
1104	58-59	0.2661457236589371
1104	60-61	0.2583382563101111
1104	62-63	0.2589805065689035
1104	64-65	0.2980665380650649
1104	66-67	0.2811295988445295
1104	68-69	0.27878330381267347
1104	70-71	0.29736396505976614
1104	72-73	0.28812759121712617
1104	74-75	0.35630399626500164
1104	76-77	0.39773186194816645
1104	78-79	0.3738745440448277
1104	80-81	0.37487209210956607
1104	82-83	0.3520543868855377
1104	84-85	0.41250902420062374
1104	86-87	0.39570922182837265
1104	88-89	0.4578137872889947
1104	90-91	0.5184061999720377
1104	92-93	0.5884961622103475
1104	94-95	0.6091230460305432
1104	96-97	0.7104908275745885
1104	98-99	0.7446429136725286
1104	100-101	0.7146515266615587
1105	1	0.1261088040856464
1105	2	0.10778385865834395
1105	3	0.10616660637424502
1105	4	0.11187959462042585
1105	5	0.08710780958396214
1105	6	0.062239226950836724
1105	7	0.08655395674518473
1105	8	0.0936117484197112
1105	9	0.11961352136858494
1105	10-11	0.07472970074271501
1105	12-13	0.09985585994309076
1105	14-15	0.09689898408286268
1105	16-17	0.11478344053502099
1105	18-19	0.10213086304879226
1105	20-21	0.09729757655770044
1105	22-23	0.11848832502003148
1105	24-25	0.09364931735466797
1105	26-27	0.10098250196223546
1105	28-29	0.10024704825305975
1105	30-31	0.09121386559220923
1105	32-33	0.10255401289725796
1105	34-35	0.09586387911792116
1105	36-37	0.10912236734301217
1105	38-39	0.09420296348836388
1105	40-41	0.14289229123438218
1105	42-43	0.14188335532460172
1105	44-45	0.11861420266713907
1105	46-47	0.11311890890910803
1105	48-49	0.10871036909322385
1105	50-51	0.10967345975257103
1105	52-53	0.14548134810750213
1105	54-55	0.12334203359821316
1105	56-57	0.10127631179943108
1105	58-59	0.13318931743211238
1105	60-61	0.14613409436388025
1105	62-63	0.18542997248125204
1105	64-65	0.14287356168197363
1105	66-67	0.13168687000565882
1105	68-69	0.12018771331155165
1105	70-71	0.17348149074667418
1105	72-73	0.19378813007953255
1105	74-75	0.2553978757877182
1105	76-77	0.28903972158949287
1105	78-79	0.26163782389657086
1105	80-81	0.27351135977665564
1105	82-83	0.2631255404226387
1105	84-85	0.34716195105797354
1105	86-87	0.38463337200050063
1105	88-89	0.39324257573384713
1105	90-91	0.43692024203948776
1105	92-93	0.512114640721304
1105	94-95	0.539708284378861
1105	96-97	0.6153396904027773
1105	98-99	0.669936207642909
1105	100-101	0.6528417520773075
1106	1	-0.020942339655874775
1106	2	0.023007916576510468
1106	3	-0.011184936064431383
1106	4	-0.001411179534791529
1106	5	-0.009502450962330045
1106	6	-0.010733340263925584
1106	7	0.001174779512531643
1106	8	0.02565280409749704
1106	9	0.00667727548160002
1106	10-11	-0.0455970861385282
1106	12-13	-0.020503968923776483
1106	14-15	-0.08022563494366608
1106	16-17	-0.08407786516735172
1106	18-19	-0.10043312513958114
1106	20-21	-0.06088320461613961
1106	22-23	-0.06441345574613422
1106	24-25	-0.0909987543586297
1106	26-27	-0.06580606148241941
1106	28-29	-0.047636503187398205
1106	30-31	-0.06339290694200628
1106	32-33	-0.059214872672470165
1106	34-35	-0.058309817713471546
1106	36-37	-0.041939581889181454
1106	38-39	-0.06080600404479242
1106	40-41	-0.04705958224572271
1106	42-43	-0.04882520359064557
1106	44-45	-0.05975057730697131
1106	46-47	-0.08110448870163367
1106	48-49	-0.06428333622201876
1106	50-51	-0.2207047651677172
1106	52-53	-0.24512298930299892
1106	54-55	-0.18681829820647522
1106	56-57	-0.2033899051739425
1106	58-59	-0.20016591702545128
1106	60-61	-0.1604617791987053
1106	62-63	-0.13601957990486824
1106	64-65	-0.140012278204388
1106	66-67	-0.1564763539232743
1106	68-69	-0.16828591949166594
1106	70-71	-0.1635695771617165
1106	72-73	-0.16909880620164586
1106	74-75	-0.112798707158003
1106	76-77	-0.06492882976989023
1106	78-79	-0.02191086655532004
1106	80-81	-0.012765172690574644
1106	82-83	-0.0031336716665215647
1106	84-85	0.057578460568606715
1106	86-87	0.09995478350220566
1106	88-89	0.04424784216267241
1106	90-91	0.08950315576652557
1106	92-93	0.20334563457869592
1106	94-95	0.2235467131388127
1106	96-97	0.25360107216651073
1106	98-99	0.3018308384850812
1106	100-101	0.23659202741424679
1107	1	-0.28533557154326417
1107	2	-0.29173209103493747
1107	3	-0.308278122981676
1107	4	-0.21433116246639372
1107	5	-0.18599460022077352
1107	6	-0.23124225171328305
1107	7	-0.17117748947303824
1107	8	-0.1376386970508463
1107	9	-0.2215715502513973
1107	10-11	-0.24117873163875458
1107	12-13	-0.28270167587353257
1107	14-15	-0.4254278077502107
1107	16-17	-0.4855541880608456
1107	18-19	-0.39468901021756153
1107	20-21	-0.45010809999842394
1107	22-23	-0.4632998021315018
1107	24-25	-0.4559296276675866
1107	26-27	-0.4761496714886704
1107	28-29	-0.5316816058927643
1107	30-31	-0.5368894430352569
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2308	48-49	-1.202696698362864
2308	50-51	-1.0697715761469553
2308	52-53	-1.0799250154160873
2308	54-55	-1.1014277927499947
2308	56-57	-1.172251249099837
2308	58-59	-1.0818289235528695
2308	60-61	-1.1092820216611017
2308	62-63	-1.033257271853877
2308	64-65	-1.1158698873813577
2308	66-67	-1.0791688780588018
2308	68-69	-1.078714186766799
2308	70-71	-1.1494766255698678
2308	72-73	-1.1562346654961573
2308	74-75	-1.1710070710625615
2308	76-77	-1.1372719666712463
2308	78-79	-1.343444125658852
2308	80-81	-1.48496692931678
2308	82-83	-1.5860288472028081
2308	84-85	-1.7884106543326048
2308	86-87	-2.0036990910358483
2308	88-89	-2.1739780783181857
2308	90-91	-2.390424252357274
2308	92-93	-2.7150529180132636
2308	94-95	-2.9892782752391653
2308	96-97	-3.421390039818082
2308	98-99	-3.8170790130968015
2308	100-101	-4.069375935328658
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	402239.0
3	105746.0
4	68722.0
5	67249.0
6	106246.0
7	184208.0
8	289634.0
9	458306.0
10	613877.0
11	544963.0
12	380568.0
13	291934.0
14	246827.0
15	225898.0
16	216574.0
17	215032.0
18	223496.0
19	227311.0
20	245971.0
21	260650.0
22	289181.0
23	333513.0
24	442630.0
25	515307.0
26	306912.0
27	337574.0
28	372121.0
29	425393.0
30	498930.0
31	599328.0
32	740975.0
33	947993.0
34	1288389.0
35	1878882.0
36	3005564.0
37	6321711.0
38	1.2463684E7
39	5650849.0
40	20899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.70993693727389	18.192427731776696	9.565472852158008	40.532162478791406
2	16.3871329354147	17.75915297359866	52.942297231752754	12.911416859233887
3	17.895501755993052	23.714940193634167	35.92703974475771	22.462518305615074
4	20.110171489291943	27.034612572530843	33.86515157135695	18.990064366820274
5	20.780416294044887	28.79715882769046	35.570948307599686	14.851476570664968
6	17.339861319216904	34.77466558082442	34.05980107341278	13.825672026545895
7	13.509385890979555	20.610318630562574	51.7041199672408	14.176175511217071
8	12.687543772528137	24.67221229261204	45.75603410033525	16.884209834524576
9	15.047542625269381	24.204195188640423	43.15094122932627	17.59732095676392
10-11	18.05319318844972	32.3610866664067	34.25973081007137	15.32598933507221
12-13	17.453801082477618	25.9127719666743	39.584071451773575	17.049355499074515
14-15	17.038329289358643	28.070300705814272	39.637826617067134	15.253543387759946
16-17	19.62167899284474	27.729231727354183	36.90670860654268	15.742380673258397
18-19	19.511299065786314	28.227072437325084	37.07980716046901	15.18182133641959
20-21	18.99507930427684	29.795136772256086	35.18290368857726	16.026880234889816
22-23	20.30365297258023	28.707696087301915	34.46039784279169	16.52825309732616
24-25	18.188615673205877	31.059452700330237	34.052774305368764	16.699157321095118
26-27	17.452976796302714	31.85019432551866	34.26607544676051	16.430753431418115
28-29	18.53969887901562	31.37050739075344	33.15931469926522	16.930479030965724
30-31	19.161546256274796	29.645739789390923	33.78051137987714	17.412202574457133
32-33	18.268708801884433	30.53713243302981	33.76929010916193	17.424868655923827
34-35	19.51128219458261	32.137286999233304	31.7623788130486	16.589051993135488
36-37	18.4564448745808	31.12402647442013	33.400851837393255	17.01867681360582
38-39	18.424950970097697	30.360480838097665	33.276477685151384	17.938090506653257
40-41	19.989687687681926	30.132714506649577	32.293529494724275	17.584068310944218
42-43	19.3912610999686	29.963392955370168	33.497770738454044	17.14757520620719
44-45	19.83187744156456	29.859262816284254	32.979502889327264	17.329356852823917
46-47	17.75118333351222	30.389480856287403	33.20199354198123	18.657342268219146
48-49	17.758718885880516	30.296786653341513	31.91668052693085	20.02781393384712
50-51	18.53332842175588	30.338437187881112	33.06468200665527	18.06355238370774
52-53	17.48936766429588	31.483360496872	33.54256816153908	17.484703677293048
54-55	18.03175590565063	31.44083371769284	31.881093102888432	18.646317273768098
56-57	18.580599083076656	31.52667570664142	33.25968602002845	16.63303919025348
58-59	18.12261907551457	32.40678864133768	32.49217815960565	16.97841412354211
60-61	18.141389894145473	34.2701498323829	29.980651294312487	17.607808979159138
62-63	17.596223576500122	34.3005402904586	30.965090258325368	17.13814587471591
64-65	17.792911758730398	32.99128250401025	31.83831876529841	17.37748697196094
66-67	17.597447501518147	34.473734223736926	30.349430745997697	17.57938752874723
68-69	17.725833962729727	32.8714862303373	31.523526282687797	17.87915352424518
70-71	17.846997746148542	33.078256728871445	31.233329206872497	17.841416318107523
72-73	18.270225257812818	33.11796108959433	30.849219755695874	17.762593896896977
74-75	18.715292709117378	32.28478250177454	31.12906930635846	17.870855482749626
76-77	18.809317410877323	32.975999667374424	30.316512950938634	17.89816997080962
78-79	18.409366703289066	32.71197612964504	31.239541359203994	17.639115807861906
80-81	19.018719935596724	32.857215810273225	30.499385333562483	17.624678920567575
82-83	18.424975549266346	32.600891668737624	31.24728388324693	17.7268488987491
84-85	18.62228258093012	32.58475637769169	30.858699293295345	17.934261748082843
86-87	18.457145565650837	32.851880886518586	30.743740499286172	17.947233048544405
88-89	18.57955378571407	32.745258709580796	30.80212453872324	17.873062965981898
90-91	18.299870160768155	33.14447300610057	30.751737341901137	17.80391949123014
92-93	19.10694428803127	32.02620606760624	30.798649145232798	18.0682004991297
94-95	19.150340322675437	33.206792656118154	30.06943422576117	17.57343279544524
96-97	19.562334453111983	32.55833989658154	30.252624355225688	17.62670129508079
98-99	19.576734898883714	32.48328524195864	29.902499088829632	18.03748077032801
100-101	19.801650613641147	33.44199788805035	29.133971813102804	17.622379685205694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40384.0
1	39832.5
2	35196.0
3	35333.5
4	44307.0
5	56113.5
6	66336.5
7	73821.5
8	82852.5
9	91898.5
10	100650.0
11	109677.5
12	118536.5
13	126857.0
14	139298.0
15	152755.0
16	162730.0
17	176590.5
18	197214.5
19	222712.5
20	259426.5
21	306121.0
22	363695.0
23	433802.0
24	513695.5
25	617318.5
26	726824.0
27	843373.0
28	962753.0
29	1080464.5
30	1252032.5
31	1495770.5
32	1665483.5
33	1816302.5
34	1877728.5
35	2111557.0
36	2215908.0
37	2097763.5
38	2137494.0
39	1925187.0
40	1742764.5
41	1666303.5
42	1451139.0
43	1254215.5
44	1135448.5
45	1022026.5
46	928282.5
47	842729.0
48	768074.5
49	701697.0
50	608602.0
51	512186.0
52	437475.5
53	373240.5
54	314503.5
55	266231.0
56	227118.0
57	189069.5
58	155430.0
59	126048.5
60	95239.0
61	67515.0
62	48579.0
63	36311.5
64	26582.0
65	17861.5
66	11905.0
67	7945.0
68	5146.5
69	3456.0
70	2654.0
71	2317.0
72	2131.5
73	2061.0
74	1773.5
75	1310.5
76	1043.0
77	867.5
78	709.5
79	502.0
80	328.0
81	234.5
82	162.5
83	114.0
84	84.5
85	58.0
86	47.5
87	39.0
88	28.5
89	23.5
90	16.0
91	14.5
92	12.0
93	7.5
94	3.5
95	2.0
96	3.0
97	3.0
98	2.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12612612526433517
2	0.15113850949148117
3	0.14756326191335867
4	0.0749391024134093
5	0.07510889677999572
6	0.14272531820062165
7	0.1427396670203332
8	0.1494668719950881
9	0.17296067280276403
10-11	0.15719131993979427
12-13	0.1756606423784833
14-15	0.1911836738364052
16-17	0.22169404748301852
18-19	0.24232406302326856
20-21	0.25967059031953055
22-23	0.26238490871496134
24-25	0.26480627204128176
26-27	0.2387727301446653
28-29	0.2270593103201542
30-31	0.2291291275635422
32-33	0.2809008648177128
34-35	0.28413174072275865
36-37	0.2906365389919849
38-39	0.28469851910136407
40-41	0.29273027093489207
42-43	0.299793477437892
44-45	0.2944891970845303
46-47	0.29213838212179155
48-49	0.06255965820728812
50-51	0.02224306202282103
52-53	0.013306138812490723
54-55	0.028637852674258883
56-57	0.03680591829504646
58-59	0.033247411006587395
60-61	0.028633069734355036
62-63	0.030541462755988322
64-65	0.03486404469408627
66-67	0.04408555282869523
68-69	0.03979525573494822
70-71	0.03813796705826668
72-73	0.03981080028963571
74-75	0.030682559483151687
76-77	0.03475284134132193
78-79	0.027823557155629643
80-81	0.04164147053783155
82-83	0.055204692370153824
84-85	0.06009046548192926
86-87	0.05035957424756105
88-89	0.045919810281818955
90-91	0.049999658019796875
92-93	0.04528248353963189
94-95	0.04191648958230251
96-97	0.04491539290201196
98-99	0.03503742626560057
100-101	0.039381531433265816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.1815286E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.65178246482958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10119317239756	27.756491209832124
2	9.218861246453383	6.388999489711006
3	3.269920630468812	3.39925735092791
4	1.7686547799290402	2.4514816275792857
5	1.0704009287872285	1.8545650067243287
6	0.7418409941852838	1.5423667652400819
7	0.5385614993207329	1.306348114287615
8	0.40562752274181885	1.1244573343837776
9	0.3085039266036473	0.9621189858793804
>10	2.1474952298337424	14.561127181079488
>50	0.23571050757685902	5.683878182633137
>100	0.16837788442488394	11.24365058259819
>500	0.014273429417954987	3.3802023332724964
>1k	0.008914313737885211	5.946369437731465
>5k	8.97557832545127E-4	2.1390553269707597
>10k+	7.663762885676544E-4	10.259631071149364
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	441398	1.0555900538382064	Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	269395	0.6442500476978682	Illumina Single End PCR Primer 1 (100% over 50bp)
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT	243580	0.582514250889017	No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT	191659	0.4583467395153055	No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT	108270	0.25892445169453104	No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT	106516	0.25472981339886086	No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA	90003	0.21523947008278266	No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG	77484	0.1853006577546785	No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG	70722	0.16912953793978594	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70446	0.16846949223305563	No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT	67980	0.16257212733161744	No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT	67574	0.16160119053113736	No Hit
ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT	66768	0.15967366574988867	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	64332	0.15384804494700816	No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA	59391	0.14203179191456444	No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT	57954	0.13859524959365338	No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT	57852	0.1383513196585574	No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA	56133	0.13424038281120448	No Hit
AGATAATACGACTCACTATAGATAATACGACTCACTATAGATAATACGAC	54983	0.1314901923664949	No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT	53195	0.12721424409245938	No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT	51333	0.12276132704198171	No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT	49329	0.11796882125833122	No Hit
GTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATA	47416	0.11339393924030557	No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA	46657	0.1115788135467972	No Hit
GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA	46565	0.11135879831122046	No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT	44829	0.10720720647468487	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	42999	0.1028308164626687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2672925398620973	0.0	0.0	1.1957349759607049E-5	0.0
2	1.2683113060616158	2.39146995192141E-6	0.0	2.3914699519214097E-5	0.0
3	1.2684141392695485	2.39146995192141E-6	0.0	3.587204927882115E-5	0.0
4	1.311974764443797	2.39146995192141E-6	0.0	3.587204927882115E-5	0.0
5	1.313005487993075	2.39146995192141E-6	0.0	4.065498918266397E-5	0.0
6	1.3138496768861034	2.39146995192141E-6	0.0	5.0220868990349605E-5	0.0
7	1.3173723121252836	2.39146995192141E-6	0.0	5.500380889419243E-5	0.0
8	1.3672272862129893	2.39146995192141E-6	0.0	5.739527884611384E-5	0.0
9	1.3677964560615465	2.39146995192141E-6	0.0	6.696115865379947E-5	0.0
10-11	1.377817910895073	3.587204927882115E-6	0.0	7.054836358168158E-5	0.0
12-13	1.3838228919443478	1.1957349759607049E-5	0.0	8.130997836532794E-5	0.0
14-15	1.3868253824689851	1.1957349759607049E-5	0.0	8.848438822109216E-5	0.0
16-17	1.3900395180843677	1.1957349759607049E-5	0.0	9.685453305281709E-5	0.0
18-19	1.3931352759371298	1.1957349759607049E-5	0.0	1.1120335276434555E-4	0.0
20-21	1.3967667230591223	1.1957349759607049E-5	0.0	1.2794364242779541E-4	0.0
22-23	1.4009565784148887	1.1957349759607049E-5	0.0	1.4468393209124528E-4	0.0
24-25	1.404507911293492	1.1957349759607049E-5	0.0	1.6381569170661656E-4	0.0
26-27	1.4085817803565903	1.1957349759607049E-5	0.0	2.3555979026425886E-4	0.0
28-29	1.41296893198339	1.434881971152846E-5	0.0	2.83389189302687E-4	0.0
30-31	1.4179109046390357	2.0327494591331985E-5	0.0	2.905635991584513E-4	0.0
32-33	1.422892336548888	2.1523229567292687E-5	0.0	2.929550691103727E-4	0.0
34-35	1.4281475917682354	2.2718964543253392E-5	0.0	2.965422740382548E-4	0.0
36-37	1.4335475309196737	2.5110434495174803E-5	0.0	3.0371668389401904E-4	0.0
38-39	1.4394078280368572	2.6306169471135508E-5	0.0	3.0849962379786184E-4	0.0
40-41	1.4462486278343283	2.7501904447096213E-5	0.0	3.3361005829303666E-4	0.0
42-43	1.4537219714340828	3.1089109374978326E-5	0.0	3.563290228362901E-4	0.0
44-45	1.4624783386630429	3.826351923074256E-5	0.0	3.8024372235550413E-4	0.0
46-47	1.4878291158883858	4.5437929086506784E-5	0.0	4.364432662256573E-4	0.0
48-49	1.4986373643360946	4.5437929086506784E-5	0.0	4.460091460333429E-4	0.0
50-51	1.50931049473152	4.5437929086506784E-5	0.0	4.4959635096122506E-4	0.0
52-53	1.5207345466918485	4.5437929086506784E-5	0.0	4.5437929086506785E-4	0.0
54-55	1.5329728941708063	4.5437929086506784E-5	0.0	4.603579657448714E-4	0.0
56-57	1.545793564583057	4.5437929086506784E-5	0.0	4.651409056487142E-4	0.0
58-59	1.5601124909201864	4.5437929086506784E-5	2.39146995192141E-6	4.8068546033620336E-4	0.0
60-61	1.5765131918504633	4.5437929086506784E-5	2.39146995192141E-6	4.938385450717711E-4	0.0
62-63	1.5941897420000908	4.663366406246749E-5	2.39146995192141E-6	5.309063293265529E-4	0.0
64-65	1.6132617148666637	4.90251340143889E-5	2.39146995192141E-6	5.643869086534527E-4	0.0
66-67	1.6338080289585966	5.141660396631031E-5	2.39146995192141E-6	5.83518668268824E-4	0.0
68-69	1.6568701694399506	5.500380889419243E-5	2.39146995192141E-6	5.91888813100549E-4	0.0
70-71	1.6842608705342825	5.739527884611384E-5	2.39146995192141E-6	6.074333677880381E-4	0.0
72-73	1.7141052197992859	5.859101382207454E-5	2.39146995192141E-6	6.122163076918809E-4	0.0
74-75	1.7483953117049111	6.217821874995665E-5	2.39146995192141E-6	6.325438022832129E-4	0.0
76-77	1.7874539946946673	6.217821874995665E-5	2.39146995192141E-6	7.389642151437156E-4	0.0
78-79	1.832560705192833	6.576542367783876E-5	2.39146995192141E-6	7.975552289657903E-4	0.0
80-81	1.8854755650840223	6.696115865379947E-5	2.39146995192141E-6	8.597334477157469E-4	0.0
82-83	1.9475724738556135	6.696115865379947E-5	2.39146995192141E-6	8.836481472349609E-4	0.0
84-85	2.0226096265370517	6.815689362976019E-5	2.39146995192141E-6	9.039756418262929E-4	0.0
86-87	2.1099090413969668	6.935262860572088E-5	2.39146995192141E-6	9.159329915858999E-4	0.0
88-89	2.2134106651811494	6.935262860572088E-5	2.39146995192141E-6	9.243031364176248E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	40390	0.0	60.572723	1
GAGCGGC	8665	0.0	59.39501	9
ATCGGGA	7150	0.0	53.639423	2
AGAGCGG	10895	0.0	48.409912	8
GCGTGTT	50535	0.0	48.018124	2
GATCGGG	8485	0.0	45.70364	1
CGCCGTA	103290	0.0	42.68003	46-47
GTCGCCG	105220	0.0	42.34265	44-45
CCGTATC	104410	0.0	42.274567	48-49
AGATCGG	123090	0.0	42.08778	1
TCGGTGG	110375	0.0	40.748043	38-39
TCTCGGT	108225	0.0	40.466385	36-37
GTTTTTT	592750	0.0	39.95138	1
GATCTCG	119875	0.0	39.295486	34-35
TGGTCGC	108655	0.0	39.27345	42-43
CCACCGA	4915	0.0	39.257183	2
GGTGGTC	109005	0.0	39.05005	40-41
GGCGCCG	8435	0.0	37.983757	44-45
CGCCGGA	5435	0.0	37.59101	46-47
GTATCAT	118930	0.0	36.986835	50-51
>>END_MODULE