FastQCFastQC Report
Fri 27 May 2016
SRR1233050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41815286
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5043151.2060541688032458TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4688991.121357868985997TruSeq Adapter, Index 6 (100% over 49bp)
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT2343590.5604625064623497No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT1856620.4440050942136328No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT1034670.2474382215154525No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT969200.23178126774022306No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA876000.2094927677883155No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG765880.18315790067775692No Hit
ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT670170.16026914176791712No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG657160.15715783936046737No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT653580.1563016931176795No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT649540.15533553925710325No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA566460.13546720689654018No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT562880.1346110606537523No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT558500.13356359681481075No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA541940.1296033225744289No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT539990.1291369859338042No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT533060.1274796972571227No Hit
AGATAATACGACTCACTATAGATAATACGACTCACTATAGATAATACGAC528420.12637005519943115No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT501590.11995374131842598No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT493300.11797121272828315No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT479640.11470446477395849No Hit
GTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATA477800.11426443430280495No Hit
GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA455820.1090079833484817No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA449970.10760897342660766No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT436390.10436135723189839No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT431700.10323975782444728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT397800.066.906291
GCGTGTT504750.052.3271522
AGATCGG1183500.044.3902631
CGGTGGT61050.042.5592421
GTTTTTT5697550.042.245221
TATGCCG1331500.041.96148346-47
TGCCGTC1332550.041.6642948-49
ACGCCAA1343250.041.41539432-33
CCGTCTT1347300.041.28593450-51
TCACGCC1369450.041.07391430-31
CTCGTAT1360450.040.8419542-43
ATCTCGT1361100.040.4471740-41
AGTCACG1346300.040.42197428-29
GCCAATA1422600.039.0318734-35
CGTCTGA1451950.038.7745516-17
CAATATC1420950.038.09951436-37
CCAGTCA1428250.038.0743826-27
ATATCTC1467400.037.65780338-39
CACGTCT1524150.037.252414-15
CGGAAGA1706600.037.147964
Produced by FastQC (version 0.11.5)