FastQCFastQC Report
Fri 27 May 2016
SRR1233049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28435111
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1316280.46290658052996525No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1201070.4223897701682965Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1071120.3766892276242565Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA811250.285298692873047No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC545450.19182270820043573Illumina Single End PCR Primer 1 (97% over 44bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT543710.19121078866194685No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530410.18653347264935943No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT495060.17410165903695612Illumina Single End PCR Primer 1 (100% over 43bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT346070.12170516935910679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT713000.072.1918261
CGCGCGT10450.059.608011
GCGTGTT953850.053.939482
ACCACCG116900.048.8108521
CCACCGA120650.047.4524652
CGGCGAC18450.046.9055941
CACCGAG128050.044.637493
ACCGAGA132850.043.027584
GCGCGTT13500.042.9720082
CGTGTTT1248650.042.12393
GAGCGGC35050.041.2526259
CCGAGAT232800.040.380651
TACGGCG9250.038.554862
CACGCGT17050.037.6494831
CCGCGTG17300.037.380271
CTCGGAG100550.037.00372740-41
CGCGTCT16050.036.1438521
CGGAGAT101650.036.13502542-43
GGCGACC24150.035.6386342
TCTCGGA106800.035.48422240-41
Produced by FastQC (version 0.11.5)