##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233049_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28435111
Sequences flagged as poor quality	0
Sequence length	101
%GC	31
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50549181960289	34.0	33.0	34.0	31.0	34.0
2	32.75956383641337	34.0	33.0	34.0	31.0	34.0
3	32.873316935530866	34.0	33.0	34.0	31.0	34.0
4	36.113685295619206	37.0	37.0	37.0	35.0	37.0
5	36.0551260024974	37.0	37.0	37.0	35.0	37.0
6	35.89894300043351	37.0	37.0	37.0	35.0	37.0
7	35.980116166945855	37.0	37.0	37.0	35.0	37.0
8	35.78808913388803	37.0	37.0	37.0	35.0	37.0
9	37.57170443259392	39.0	39.0	39.0	35.0	39.0
10-11	37.716841038531555	39.0	39.0	39.0	36.0	39.0
12-13	37.78108043960159	39.0	39.0	39.0	37.0	39.0
14-15	39.34580589469125	41.0	40.0	41.0	37.5	41.0
16-17	39.15950136435198	41.0	39.5	41.0	37.0	41.0
18-19	38.75241556117013	41.0	39.0	41.0	36.0	41.0
20-21	38.38368728365435	41.0	39.0	41.0	35.0	41.0
22-23	37.94967797030931	41.0	38.0	41.0	34.5	41.0
24-25	37.52454636101122	41.0	38.0	41.0	33.5	41.0
26-27	37.096672912583315	41.0	38.0	41.0	32.0	41.0
28-29	36.56830295123518	41.0	38.0	41.0	28.5	41.0
30-31	36.070146745690565	41.0	38.0	41.0	23.5	41.0
32-33	35.715850783912884	41.0	37.5	41.0	17.5	41.0
34-35	35.4399543226682	41.0	37.0	41.0	13.5	41.0
36-37	35.168609452588385	40.0	37.0	41.0	9.0	41.0
38-39	35.00025433345416	40.0	36.0	41.0	7.5	41.0
40-41	34.893285874635765	40.0	36.0	41.0	7.0	41.0
42-43	34.90530028175378	40.0	36.0	41.0	3.5	41.0
44-45	34.818399900039076	40.0	35.5	41.0	2.0	41.0
46-47	34.58054452468991	40.0	35.0	41.0	2.0	41.0
48-49	34.446074801677405	40.0	35.0	41.0	2.0	41.0
50-51	33.902326686890724	39.5	35.0	40.5	2.0	41.0
52-53	33.953965679965165	40.0	35.0	41.0	2.0	41.0
54-55	34.02585222895736	40.0	35.0	41.0	2.0	41.0
56-57	33.93833792314017	40.0	35.0	41.0	2.0	41.0
58-59	33.702386883596134	40.0	35.0	41.0	2.0	41.0
60-61	33.47263370978225	40.0	35.0	41.0	2.0	41.0
62-63	33.25561540800737	39.0	35.0	41.0	2.0	41.0
64-65	32.960792838121854	39.0	35.0	41.0	2.0	41.0
66-67	32.54311265041308	39.0	34.0	41.0	2.0	41.0
68-69	32.24685157726306	38.5	34.0	41.0	2.0	41.0
70-71	31.82924933895985	37.5	34.0	40.0	2.0	41.0
72-73	31.457076235784697	37.0	33.0	40.0	2.0	41.0
74-75	31.066226047086644	36.5	33.0	39.5	2.0	41.0
76-77	30.538434332118484	36.0	32.0	39.0	2.0	41.0
78-79	30.099447844603105	35.5	31.0	39.0	2.0	40.5
80-81	29.708807062508036	35.0	31.0	37.5	2.0	39.5
82-83	29.269058946173974	35.0	31.0	37.0	2.0	39.0
84-85	28.823020842084983	35.0	30.0	37.0	2.0	39.0
86-87	28.390874788566855	35.0	29.5	36.0	2.0	37.5
88-89	27.894788101934964	35.0	29.0	36.0	2.0	37.0
90-91	27.374711074628827	34.5	27.0	35.0	2.0	37.0
92-93	26.89121751626009	34.0	25.5	35.0	2.0	36.0
94-95	26.440296065663325	34.0	24.0	35.0	2.0	36.0
96-97	25.906703986490506	34.0	19.0	35.0	2.0	36.0
98-99	25.320449056801642	34.0	3.5	35.0	2.0	35.0
100-101	23.699023471369607	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23741116036018894
1101	2	0.22050265918095135
1101	3	0.1988877575261867
1101	4	0.15420494019237196
1101	5	0.1646711623381094
1101	6	0.24624581223316966
1101	7	0.10410388575054697
1101	8	0.09755284662097807
1101	9	0.15060740166113362
1101	10-11	0.1146661479838258
1101	12-13	0.14305912763349227
1101	14-15	0.1989410896535091
1101	16-17	0.22867315716766257
1101	18-19	0.2453156213675527
1101	20-21	0.19948508793294906
1101	22-23	0.27814943221936517
1101	24-25	0.26378374808071925
1101	26-27	0.25089764581361607
1101	28-29	0.20830295738298332
1101	30-31	0.2731805609058995
1101	32-33	0.21619801167863528
1101	34-35	0.23487494573655
1101	36-37	0.2162335235622166
1101	38-39	0.2295414178221904
1101	40-41	0.23615386399895044
1101	42-43	0.2917123380316724
1101	44-45	0.2915634074573106
1101	46-47	0.2741551708370551
1101	48-49	0.2323107796583841
1101	50-51	0.11637316419236043
1101	52-53	0.15467922493127162
1101	54-55	0.21928620238697505
1101	56-57	0.24354485665002557
1101	58-59	0.2931009342589377
1101	60-61	0.33801851275313766
1101	62-63	0.31036794645356025
1101	64-65	0.24636891327640598
1101	66-67	0.24496775405325621
1101	68-69	0.29266955282255225
1101	70-71	0.22035322918936018
1101	72-73	0.2308570495243849
1101	74-75	0.25033812408726774
1101	76-77	0.39788879555270285
1101	78-79	0.31829310581722936
1101	80-81	0.3277484684337644
1101	82-83	0.3699158882399125
1101	84-85	0.3696417876926894
1101	86-87	0.35522966484513674
1101	88-89	0.35250041693802814
1101	90-91	0.466613711886378
1101	92-93	0.4763539548798441
1101	94-95	0.6336119395986586
1101	96-97	0.6548428867346097
1101	98-99	0.6655763723671377
1101	100-101	0.8097667689396317
1102	1	0.10424756960800607
1102	2	0.14616231431862303
1102	3	0.16729727582411869
1102	4	0.1389990582953189
1102	5	0.13574839550889095
1102	6	0.2524887871022585
1102	7	0.11911599824561847
1102	8	0.03057002732829517
1102	9	0.044340959841854044
1102	10-11	0.08545311408239797
1102	12-13	0.12015640895281621
1102	14-15	0.18200802268115268
1102	16-17	0.23051321089902643
1102	18-19	0.22115210746868996
1102	20-21	0.2547288472011715
1102	22-23	0.2629249335881383
1102	24-25	0.21917391827131638
1102	26-27	0.19940680290132207
1102	28-29	0.11512910277804167
1102	30-31	0.13030730873425966
1102	32-33	0.1410793844273357
1102	34-35	0.15425907639422576
1102	36-37	0.15948133773795092
1102	38-39	0.15865906609717229
1102	40-41	0.21914215057325492
1102	42-43	0.20425530035786466
1102	44-45	0.20099434127934046
1102	46-47	0.17225519883105989
1102	48-49	0.0993998969054104
1102	50-51	0.09798848082288458
1102	52-53	0.1512046358869057
1102	54-55	0.1784482730698116
1102	56-57	0.17077792926161806
1102	58-59	0.15093087842887343
1102	60-61	0.15406539823865728
1102	62-63	0.12583070713748867
1102	64-65	0.10886091369565776
1102	66-67	0.16236404942328164
1102	68-69	0.21838834723151734
1102	70-71	0.13913521617680757
1102	72-73	0.14592191680717193
1102	74-75	0.1352821084475515
1102	76-77	0.277510886776259
1102	78-79	0.27955604578626847
1102	80-81	0.23686985684847173
1102	82-83	0.25241532183908433
1102	84-85	0.2623239931868575
1102	86-87	0.26822468973133695
1102	88-89	0.34475174043118884
1102	90-91	0.4556908764289318
1102	92-93	0.4815690658580998
1102	94-95	0.5227963822348123
1102	96-97	0.5517043238997594
1102	98-99	0.6370888331831956
1102	100-101	0.8770487168312044
1103	1	0.1774658021467701
1103	2	0.16890049387104966
1103	3	0.15977218541491567
1103	4	0.13737725980796256
1103	5	0.11559749594704272
1103	6	0.17188226676630336
1103	7	0.07576720447737983
1103	8	0.07217771001824502
1103	9	0.05654884676238936
1103	10-11	0.050914419135210665
1103	12-13	0.0976901856606247
1103	14-15	0.06918389187399754
1103	16-17	0.11881821424797323
1103	18-19	0.11545333290316506
1103	20-21	0.09853751028945368
1103	22-23	0.1196510678123488
1103	24-25	0.1453277044657355
1103	26-27	0.12031199002505133
1103	28-29	0.11847980452907336
1103	30-31	0.10635831818069619
1103	32-33	0.12453369477007215
1103	34-35	0.1488405222162683
1103	36-37	0.14530652287995593
1103	38-39	0.11898246619744413
1103	40-41	0.15905792844804267
1103	42-43	0.17933578705270747
1103	44-45	0.19022180889566442
1103	46-47	0.187073091486468
1103	48-49	0.20001174256204735
1103	50-51	0.1389795086665444
1103	52-53	0.17589278811183107
1103	54-55	0.23429143563965482
1103	56-57	0.21926930709838643
1103	58-59	0.24277656862955865
1103	60-61	0.2559959830620784
1103	62-63	0.25407987544850386
1103	64-65	0.2593674329777613
1103	66-67	0.23476151893128616
1103	68-69	0.21680006992715306
1103	70-71	0.191965742976965
1103	72-73	0.2077519217767403
1103	74-75	0.23053689747519357
1103	76-77	0.3750538727789241
1103	78-79	0.3356463721548941
1103	80-81	0.32503455672069137
1103	82-83	0.34707572865228187
1103	84-85	0.3932576705589135
1103	86-87	0.3632746868336021
1103	88-89	0.41910464677826553
1103	90-91	0.5382270647954392
1103	92-93	0.5453714726653693
1103	94-95	0.6569440888592872
1103	96-97	0.6811138382854516
1103	98-99	0.6837797324780794
1103	100-101	0.8893668636288581
1104	1	0.1646684158075189
1104	2	0.1619077480479021
1104	3	0.13359348675887617
1104	4	0.12157704679940196
1104	5	0.09666296819825249
1104	6	0.19464902036515497
1104	7	0.07907092636560265
1104	8	0.0904140716250339
1104	9	0.11518726703273785
1104	10-11	0.07138856654603387
1104	12-13	0.0705164624093797
1104	14-15	0.0825414360072898
1104	16-17	0.09541503908210558
1104	18-19	0.1287493400142381
1104	20-21	0.10803685836282284
1104	22-23	0.09794669782175447
1104	24-25	0.10335063771464092
1104	26-27	0.06716915537928259
1104	28-29	0.06556078943164323
1104	30-31	0.05360305238346541
1104	32-33	0.04533652356075635
1104	34-35	0.04745428425443521
1104	36-37	0.0853204367021334
1104	38-39	0.0917377520988154
1104	40-41	0.074707141254855
1104	42-43	0.05985271992052077
1104	44-45	0.06905284013033253
1104	46-47	0.06532016486456627
1104	48-49	0.09203775154493599
1104	50-51	0.02039604035235243
1104	52-53	-0.013448657449998791
1104	54-55	0.046196460444591025
1104	56-57	0.05106878307213236
1104	58-59	0.08219028650754723
1104	60-61	0.16763235230816065
1104	62-63	0.15032636961600332
1104	64-65	0.09590624655503888
1104	66-67	0.10951419725360978
1104	68-69	0.07872388307350064
1104	70-71	0.08520969840375514
1104	72-73	0.06745984763193036
1104	74-75	0.0925166752588602
1104	76-77	0.21422926751095517
1104	78-79	0.20395666473648433
1104	80-81	0.1261177980935635
1104	82-83	0.1436833824775512
1104	84-85	0.06621584939888336
1104	86-87	-0.001958597277035068
1104	88-89	0.010631640842547085
1104	90-91	0.05687715334335053
1104	92-93	-0.02692157741426726
1104	94-95	-0.10113931895869754
1104	96-97	-0.18715932880318675
1104	98-99	-0.2135470490980218
1104	100-101	-0.09777871066463106
1105	1	0.10993683391448172
1105	2	0.09717932459008694
1105	3	0.07769598121455346
1105	4	0.05385557697317722
1105	5	0.0545690564489405
1105	6	0.14039119055733096
1105	7	0.050787406958868075
1105	8	0.0925191761468298
1105	9	0.12077245951308413
1105	10-11	0.06712230119082108
1105	12-13	0.024869560946747526
1105	14-15	0.031211415319013724
1105	16-17	-0.0037984686429410885
1105	18-19	-0.0030063272764238036
1105	20-21	0.01645881881991329
1105	22-23	0.046241431681480094
1105	24-25	0.01542908396471887
1105	26-27	-2.694619853471636E-4
1105	28-29	8.761993037609273E-4
1105	30-31	-0.025719068919343613
1105	32-33	0.003528584649700406
1105	34-35	-0.019732686997741666
1105	36-37	-0.01988946545018422
1105	38-39	0.005620348781214091
1105	40-41	-9.176580496728093E-5
1105	42-43	0.011910004834767562
1105	44-45	0.03005271915899499
1105	46-47	0.02366534391290287
1105	48-49	0.03355542438113446
1105	50-51	-0.10114433289560765
1105	52-53	-0.023643614096990007
1105	54-55	0.04948526601329917
1105	56-57	0.03053436262370468
1105	58-59	0.021183908028376663
1105	60-61	0.08649047696808054
1105	62-63	0.10484786806785706
1105	64-65	0.10502598538577246
1105	66-67	0.0924723818498947
1105	68-69	0.10725303451499002
1105	70-71	0.09556033587952228
1105	72-73	0.09603314286289688
1105	74-75	0.09085989211105172
1105	76-77	0.2030534738973664
1105	78-79	0.18725277393201267
1105	80-81	0.1783282174944425
1105	82-83	0.1014466338002542
1105	84-85	0.07309248759591824
1105	86-87	-0.002233984649887333
1105	88-89	-0.0028161439322431647
1105	90-91	0.10602126509627041
1105	92-93	0.08143592314793935
1105	94-95	0.13331781195568126
1105	96-97	0.14581292219290987
1105	98-99	0.15673931893630666
1105	100-101	0.245746916498625
1106	1	0.022635036606608594
1106	2	0.017709929895424636
1106	3	-0.010250602367960937
1106	4	-0.014395736623157518
1106	5	0.007630867300477462
1106	6	0.05661281289616227
1106	7	-0.01254816984226892
1106	8	0.007524835022202581
1106	9	0.03375195928695973
1106	10-11	-0.027475939189088194
1106	12-13	-0.07248334257729994
1106	14-15	-0.11542004003603523
1106	16-17	-0.09044188474271664
1106	18-19	-0.10027021180886209
1106	20-21	-0.09675101299584554
1106	22-23	-0.088800632944384
1106	24-25	-0.10072745594528243
1106	26-27	-0.09640703395913164
1106	28-29	-0.05062439121416418
1106	30-31	-0.09314868948126076
1106	32-33	-0.13034414861960641
1106	34-35	-0.13773944508091773
1106	36-37	-0.09921055776348453
1106	38-39	-0.08795466430818522
1106	40-41	-0.0806289134393765
1106	42-43	-0.1034373722933779
1106	44-45	-0.09621060669179116
1106	46-47	-0.08257057225873865
1106	48-49	-0.057845789275617676
1106	50-51	-0.2018362027539311
1106	52-53	-0.21442293009306468
1106	54-55	-0.16668050778535104
1106	56-57	-0.13216971365444152
1106	58-59	-0.10032401218099807
1106	60-61	-0.07648051644908804
1106	62-63	-0.08817119368565329
1106	64-65	-0.13867376881258764
1106	66-67	-0.1183588408885825
1106	68-69	-0.1117511823986348
1106	70-71	-0.13129836613197554
1106	72-73	-0.11926629122179122
1106	74-75	-0.09561571393386004
1106	76-77	0.05568413730342314
1106	78-79	-0.0023371999626320417
1106	80-81	0.005727629269440371
1106	82-83	-0.01389713966274897
1106	84-85	0.04913309300526336
1106	86-87	0.012443635000533249
1106	88-89	-0.04097418437494582
1106	90-91	0.09651004997727952
1106	92-93	0.10295138566668882
1106	94-95	0.15235554674038454
1106	96-97	0.11620015042327836
1106	98-99	0.11546497415905677
1106	100-101	0.23863235520679282
1107	1	-0.06791796384526094
1107	2	-0.05525563202632355
1107	3	-0.06879037101848695
1107	4	-0.0774522365874617
1107	5	-0.07467733621049177
1107	6	0.03449744404706934
1107	7	-0.020760979263137358
1107	8	-0.03593693483799143
1107	9	-0.08876328633067487
1107	10-11	-0.1096644673869065
1107	12-13	-0.14307755931977795
1107	14-15	-0.19237877817196392
1107	16-17	-0.20156089287657863
1107	18-19	-0.23844203213179327
1107	20-21	-0.24712653562250608
1107	22-23	-0.22727273823991112
1107	24-25	-0.25020526955945144
1107	26-27	-0.37665709065449704
1107	28-29	-0.306911533135775
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2308	30-31	-0.6329854970751114
2308	32-33	-0.6528306046210375
2308	34-35	-0.6067310894516496
2308	36-37	-0.656066280754942
2308	38-39	-0.6592083930582078
2308	40-41	-0.5808435446862816
2308	42-43	-0.6817947824106696
2308	44-45	-0.6098832124952125
2308	46-47	-0.5605698893170867
2308	48-49	-0.6379382777987601
2308	50-51	-0.5872905412068334
2308	52-53	-0.5372269193963533
2308	54-55	-0.5664114326775902
2308	56-57	-0.660341486070493
2308	58-59	-0.7712034106593606
2308	60-61	-0.6072897500420709
2308	62-63	-0.5728497804280792
2308	64-65	-0.5837145873355425
2308	66-67	-0.5413854259528392
2308	68-69	-0.5824293469470838
2308	70-71	-0.617118089998506
2308	72-73	-0.6354496747627607
2308	74-75	-0.6917721146131335
2308	76-77	-0.5498015588466636
2308	78-79	-0.6179006547354682
2308	80-81	-0.7736212274409198
2308	82-83	-0.7686154214609857
2308	84-85	-0.9239575550891104
2308	86-87	-1.0447023058100768
2308	88-89	-1.1032327152999457
2308	90-91	-1.1837022268800759
2308	92-93	-1.4708295456105205
2308	94-95	-1.76692337919361
2308	96-97	-2.2371428375793236
2308	98-99	-2.61173157586067
2308	100-101	-3.035870975739492
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129469.0
3	35109.0
4	26204.0
5	28194.0
6	42792.0
7	78050.0
8	157042.0
9	314127.0
10	511323.0
11	546544.0
12	446716.0
13	348271.0
14	257270.0
15	212777.0
16	185186.0
17	176255.0
18	182137.0
19	174260.0
20	180022.0
21	183934.0
22	192260.0
23	198429.0
24	220635.0
25	278973.0
26	217855.0
27	236136.0
28	237634.0
29	256463.0
30	291395.0
31	340004.0
32	409990.0
33	510474.0
34	690151.0
35	1207763.0
36	1932141.0
37	4310888.0
38	8618671.0
39	4062275.0
40	7292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.454479436759556	17.066770414268202	10.595395201163942	41.8833549478083
2	15.80358149995417	18.975832798046575	52.46485196881605	12.755733733183206
3	18.330866024645097	22.25247436518639	40.196297923896054	19.22036168627246
4	19.510493669298917	28.384446857789968	36.315107292997084	15.78995217991403
5	19.091772267683837	31.43538678455709	36.69082783710812	12.78201311065095
6	16.641559333168395	33.221132734056106	37.70234417347039	12.434963759305115
7	12.060573160844584	18.81916881102612	56.54109767704184	12.579160351087454
8	12.487205187028987	22.86546141802095	49.23503955984504	15.412293835105025
9	14.211256177531991	21.592378913991226	49.07258614855651	15.12377875992027
10-11	15.848110638891393	32.441092037809575	39.14547007658647	12.565327246712563
12-13	15.343449063597959	25.458729257105034	44.96548362888346	14.232338050413547
14-15	15.209555061953534	27.308126837568025	43.93923315491717	13.543084945561274
16-17	16.575059908730204	27.451155890289407	42.0526251002887	13.921159100691682
18-19	16.47144724504522	28.10487762772466	41.305726108937044	14.117949018293071
20-21	16.705247782472807	28.78476513319907	40.33743178214703	14.172555302181097
22-23	17.063628196078234	28.97881484732575	40.07149177250401	13.886065184092011
24-25	16.1580852374218	30.365307494240863	39.070574600658304	14.406032667679034
26-27	16.50465947666855	30.632494055857013	38.21756929931392	14.645277168160517
28-29	16.989530165341233	30.959873389554502	37.337686328282985	14.712910116821284
30-31	17.071593943494264	30.988247092187386	37.24837290337817	14.691786060940181
32-33	16.262733853228887	31.666026245267798	37.08465083525218	14.98658906625114
34-35	16.744155505175037	32.43225319876807	35.84725436556731	14.976336930489579
36-37	15.948974299935228	32.21491899337806	36.42320613302364	15.412900573663075
38-39	16.072723865862717	31.57647537642226	36.6238117535708	15.72698900414423
40-41	16.80166609319358	31.39479324536011	36.69174268778607	15.11179797366024
42-43	16.297594035910464	31.507171692926487	37.352000534523846	14.843233736639197
44-45	17.105269587148765	30.624643862846295	37.20541725546216	15.064669294542782
46-47	15.975814593365511	31.72001746991918	36.620858604442304	15.683309332272998
48-49	15.695767965842345	31.514973178735	36.72376674378475	16.065492111637912
50-51	16.328060535227618	31.620081985867714	36.94771415271034	15.10414332619433
52-53	15.83432408059908	32.69069807010193	36.55981730252326	14.915160546775732
54-55	16.03252401581974	33.090514399609695	35.21988009823489	15.657081486335677
56-57	16.053624689560735	32.880077380390745	35.874138138585074	15.192159791463448
58-59	15.680807435567951	34.348897037890936	34.76403520985025	15.206260316690868
60-61	15.55507379591379	34.752466765471745	34.30341981081065	15.389039627803811
62-63	15.550871245060376	35.37203178141278	33.89289037767428	15.18420659585257
64-65	15.580222282234102	34.94484336635788	34.22154743830612	15.253386913101904
66-67	15.896823824601917	35.48582068134005	33.616019645571285	15.001335848486752
68-69	16.201786587012094	35.142892531701385	33.75364175648901	14.901679124797509
70-71	15.569016066088153	35.48879235955857	33.34113061841046	15.601060955942813
72-73	15.839259428247003	35.181000348477625	33.57046153257499	15.409278690700381
74-75	15.997122360450783	35.06339750177167	33.32374366324788	15.615736474529676
76-77	15.78723210757631	35.006895349228145	33.56391683126366	15.641955711931885
78-79	15.798231038650453	35.55094289889968	33.29357195552426	15.357254106925616
80-81	15.873245580085833	35.71012611837527	33.29138753845554	15.125240763083358
82-83	15.995360454193408	35.28108084403117	33.205548239287694	15.518010462487734
84-85	15.858144531245191	35.2312850123919	33.28873940390104	15.621831052461864
86-87	16.434963098965923	35.58614559303109	32.63245394048224	15.346437367520737
88-89	16.410936465132842	35.448073334406885	32.87373311115262	15.267257089307652
90-91	16.188445686039348	35.82069540716757	32.368020297160086	15.622838609633
92-93	16.635614076042792	35.615500339123194	32.30654169808618	15.442343886747834
94-95	16.78894448486591	35.897164248804934	31.362789475307483	15.95110179102167
96-97	16.425936869952242	36.232697504648876	32.02901913837359	15.312346487025286
98-99	16.820013517095877	36.025008807775684	31.49412300332174	15.660854671806698
100-101	17.290449472836592	36.41770556126895	30.578725365271126	15.71311960062333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82376.0
1	101017.5
2	106206.0
3	109726.0
4	143824.5
5	179459.5
6	195437.5
7	196292.5
8	203868.0
9	211286.5
10	219190.0
11	223330.5
12	228032.5
13	231749.0
14	246044.5
15	255089.5
16	247243.5
17	250415.5
18	263234.5
19	282202.0
20	311807.5
21	354639.5
22	409424.5
23	473631.5
24	555299.5
25	670515.0
26	788604.5
27	885076.0
28	961532.5
29	1037774.5
30	1102504.0
31	1102693.0
32	1081426.5
33	1089711.5
34	1107338.0
35	1089055.0
36	1067024.5
37	1040654.0
38	1017021.0
39	1003386.0
40	963801.5
41	890990.0
42	768688.5
43	659392.5
44	579452.0
45	513859.0
46	464158.5
47	419453.0
48	378342.5
49	331621.5
50	272740.5
51	215531.5
52	172858.5
53	141484.0
54	122396.0
55	124793.5
56	104889.5
57	65938.0
58	46447.0
59	32657.0
60	22883.0
61	16516.5
62	12035.0
63	8462.5
64	5856.5
65	4074.5
66	2894.5
67	2075.0
68	1475.0
69	1080.0
70	840.5
71	679.5
72	570.0
73	473.5
74	369.5
75	282.5
76	220.5
77	197.0
78	164.0
79	116.5
80	90.5
81	74.5
82	61.5
83	49.5
84	28.0
85	22.5
86	18.0
87	8.5
88	11.5
89	9.5
90	5.5
91	5.0
92	3.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14263703771017458
2	0.1704125579112387
3	0.1589689591857053
4	0.12203926336000588
5	0.13326482179021562
6	0.21428085861876892
7	0.2324977736151619
8	0.23278614948962217
9	0.25621141412108434
10-11	0.2536898836090353
12-13	0.24344374811830347
14-15	0.26413295872134984
16-17	0.277303295914688
18-19	0.29203156618590304
20-21	0.29776391588554024
22-23	0.3053056483584678
24-25	0.29799250651773435
26-27	0.28094843730344504
28-29	0.2428230366324225
30-31	0.24109805655409608
32-33	0.2907866264351843
34-35	0.30310941989992585
36-37	0.309420279737962
38-39	0.3021985741501062
40-41	0.30214933924471055
42-43	0.31083754165756555
44-45	0.31709916659020604
46-47	0.3098264677074762
48-49	0.04991012695536866
50-51	0.022187358438657052
52-53	0.0012132887401072568
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.0550336870497886E-5
78-79	2.233154637588719E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	5.9785242266154686E-5
94-95	0.0
96-97	5.0993294874073114E-5
98-99	3.165101061149366E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.8435111E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.70269439325346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8250622059265	33.3050050067678
2	8.572592063737444	6.978551898566704
3	3.039786486427678	3.7118250113342235
4	1.5805919496702914	2.5733740435146606
5	0.9841158658090728	2.002808366678936
6	0.6503888571948195	1.5883547334706865
7	0.47326296479863783	1.3484154476690817
8	0.3579311981131858	1.1655011336489647
9	0.2766352238226697	1.013381907629705
>10	1.8765088378780164	14.82652145872595
>50	0.2000042551660737	5.676302776931969
>100	0.14096289793138764	11.154800370946155
>500	0.014124598254108191	3.942121966662894
>1k	0.0072444709405633185	5.629563900255709
>5k	5.283031216807197E-4	1.4269277519377817
>10k+	2.598212073721144E-4	3.6565442252590437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131628	0.46290658052996525	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	120107	0.4223897701682965	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	107112	0.3766892276242565	Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81125	0.285298692873047	No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGAGATC	54545	0.19182270820043573	Illumina Single End PCR Primer 1 (97% over 44bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	54371	0.19121078866194685	No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53041	0.18653347264935943	No Hit
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	49506	0.17410165903695612	Illumina Single End PCR Primer 1 (100% over 43bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	34607	0.12170516935910679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.4349552213810595	3.5167789568326286E-6	0.0	1.7583894784163143E-5	0.0
2	0.44075790665983333	1.0550336870497886E-5	0.0	2.46174526978284E-5	0.0
3	0.4409583630603728	1.0550336870497886E-5	0.0	3.8684568525158914E-5	0.0
4	0.7258913109219092	1.0550336870497886E-5	0.0	3.8684568525158914E-5	0.0
5	0.7306917141979857	1.0550336870497886E-5	0.0	5.275168435248943E-5	0.0
6	0.7325169224765818	1.0550336870497886E-5	0.0	6.681880017981994E-5	0.0
7	0.7484795821616451	1.0550336870497886E-5	0.0	8.791947392081571E-5	0.0
8	0.9530435805226855	1.0550336870497886E-5	0.0	8.791947392081571E-5	0.0
9	0.9545593122530803	1.0550336870497886E-5	0.0	1.0550336870497886E-4	0.0
10-11	1.0283958448412598	1.0550336870497886E-5	0.0	1.1077853714022779E-4	0.0
12-13	1.0686418632232524	1.0550336870497886E-5	0.0	1.1605370557547674E-4	0.0
14-15	1.0835020127053485	1.0550336870497886E-5	0.0	1.353959898380562E-4	0.0
16-17	1.09356000052189	1.4067115827330514E-5	0.0	1.4594632670855408E-4	0.0
18-19	1.0988545112414014	1.4067115827330514E-5	0.0	1.600134425358846E-4	0.0
20-21	1.1032487265479638	1.4067115827330514E-5	0.0	1.8463089523371298E-4	0.0
22-23	1.1070855323898683	1.4067115827330514E-5	0.0	2.0045640053945981E-4	0.0
24-25	1.110011492481953	1.582550530574683E-5	0.0	2.2507385323728823E-4	0.0
26-27	1.1131256002482284	1.7583894784163143E-5	0.0	2.971678218523571E-4	0.0
28-29	1.116380379172777	1.7583894784163143E-5	0.0	3.3761077985593234E-4	0.0
30-31	1.1204668763206165	1.7583894784163143E-5	0.0	3.481611167264302E-4	0.0
32-33	1.1242931318256502	1.7583894784163143E-5	0.0	3.5167789568326286E-4	0.0
34-35	1.1283831457524467	1.7583894784163143E-5	0.0	3.6222823255376073E-4	0.0
36-37	1.1329250657751961	1.7583894784163143E-5	0.0	3.798121273379239E-4	0.0
38-39	1.1382283684420997	1.7583894784163143E-5	0.0	3.798121273379239E-4	0.0
40-41	1.1445392282801357	1.7583894784163143E-5	0.0	3.9915441160050334E-4	0.0
42-43	1.152497699059448	2.2859063219412086E-5	0.0	4.25530253776748E-4	0.0
44-45	1.162415015717716	2.813423165466103E-5	0.0	4.483893169961601E-4	0.0
46-47	1.21939210998684	3.69261790467426E-5	0.0	4.835571065644864E-4	0.0
48-49	1.236536407401399	3.8684568525158914E-5	0.0	5.011410013486496E-4	0.0
50-51	1.2516128387893404	5.9785242266154686E-5	0.0	5.046577803054823E-4	0.0
52-53	1.2649256055304303	8.264430548556676E-5	0.0	5.064161697838985E-4	0.0
54-55	1.2785619159355488	1.0374497922656254E-4	0.0	5.134497276975638E-4	0.0
56-57	1.2926430988787068	1.1605370557547674E-4	0.0	5.20483285611229E-4	0.0
58-59	1.3097856379037873	1.2836243192439092E-4	0.0	5.240000645680616E-4	0.0
60-61	1.3339248086634865	1.371543793164725E-4	0.0	5.292752330033106E-4	0.0
62-63	1.354956905214824	1.371543793164725E-4	0.0	5.433423488306411E-4	0.0
64-65	1.3789501296478146	1.3891276879488882E-4	0.0	5.538926857011389E-4	0.0
66-67	1.4034568038085027	1.4946310566538672E-4	0.0	5.626846330932206E-4	0.0
68-69	1.4301755319330387	1.5825505305746827E-4	0.0	5.626846330932206E-4	0.0
70-71	1.4626529855993882	1.600134425358846E-4	0.0	5.662014120500532E-4	0.0
72-73	1.4993892585824615	1.6704700044954985E-4	0.0	5.767517489205511E-4	0.0
74-75	1.5414499349061799	1.6880538992796617E-4	0.0	5.943356437047142E-4	0.0
76-77	1.5914778739566025	1.740805583632151E-4	0.0	6.1543631744571E-4	0.0
78-79	1.6487503776581003	1.7935572679846404E-4	0.0	6.224698753593752E-4	0.0
80-81	1.7155111509851326	1.8287250575529669E-4	0.0	6.330202122298732E-4	0.0
82-83	1.7938544358064927	1.8463089523371298E-4	0.0	6.506041070140362E-4	0.0
84-85	1.8894738972532936	1.8990606366896194E-4	0.0	6.506041070140362E-4	0.0
86-87	2.0041648509829977	1.9166445314737826E-4	0.0	6.541208859708689E-4	0.0
88-89	2.142284586123121	1.9342284262579456E-4	0.0	6.541208859708689E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	71300	0.0	72.191826	1
CGCGCGT	1045	0.0	59.60801	1
GCGTGTT	95385	0.0	53.93948	2
ACCACCG	11690	0.0	48.810852	1
CCACCGA	12065	0.0	47.452465	2
CGGCGAC	1845	0.0	46.905594	1
CACCGAG	12805	0.0	44.63749	3
ACCGAGA	13285	0.0	43.02758	4
GCGCGTT	1350	0.0	42.972008	2
CGTGTTT	124865	0.0	42.1239	3
GAGCGGC	3505	0.0	41.252625	9
CCGAGAT	23280	0.0	40.38065	1
TACGGCG	925	0.0	38.55486	2
CACGCGT	1705	0.0	37.649483	1
CCGCGTG	1730	0.0	37.38027	1
CTCGGAG	10055	0.0	37.003727	40-41
CGCGTCT	1605	0.0	36.143852	1
CGGAGAT	10165	0.0	36.135025	42-43
GGCGACC	2415	0.0	35.638634	2
TCTCGGA	10680	0.0	35.484222	40-41
>>END_MODULE