FastQCFastQC Report
Fri 27 May 2016
SRR1233049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28435111
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1913830.6730517070954989TruSeq Adapter, Index 5 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1091740.3839408258332454No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1030210.3623020849118542TruSeq Adapter, Index 5 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA785030.2760776984482319No Hit
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC480310.168914410075628TruSeq Adapter, Index 5 (100% over 42bp)
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448380.15768533486646139No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT424450.1492696828227609No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT330430.11620492707062054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT107250.079.100721
CGCGTGT685700.074.474281
GTGGTCG42600.067.827333
CGGAGAT74650.067.66661
GTCGCCG16450.065.150816
TCGGCAC29950.057.380517
GATCGGC35550.057.2398225
CCGAGAT84800.056.5527041
CGGCACT27350.055.4124768
GCGTGTT929350.054.8868332
CGCCGTA5950.054.750998
GGTAGAT102300.053.997155
GGTCGCC20850.052.987135
GGTGGTC59750.050.334352
TCGCCGA16600.050.0569577
CGCGCGT13600.049.777941
CGCCGAG18300.049.0187648
GTGGTAG112650.048.156153
GGTGGTA112900.047.7149662
GGTCGAG24600.046.445215
Produced by FastQC (version 0.11.5)