FastQCFastQC Report
Fri 27 May 2016
SRR1233048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38114768
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT2593360.6804081819414459No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1148410.3013031589225468Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA946570.24834730726945525No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT943600.24756808174721148No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA910420.23886279459972048No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT883740.23186288317431186No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT871130.22855445427347218No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG820500.21527088922592943No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG805590.2113590196849683No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT643350.16879284166179365No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC636010.16686707892331917No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT619540.16254591920905828No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT610350.1601347803035296No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA595860.15633310427076455No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC570960.14980020342771075Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA549010.1440412807969866No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT481620.12636047004142856No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT446460.11713569921244174No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA430620.11297982976047499No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC392850.1030702849876982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT407100.064.437261
GAGCGGC61500.046.89949
GCGTGTT558750.046.7623862
ACCACCG53200.044.290631
CCACCGA56950.041.7088052
GTTTTTT5747700.040.930391
ATCGGGA62400.040.5021932
ACCGAGA64150.037.1012544
CCCGAAA73850.036.9235271
CCGAGAT112950.035.9601631
CGTGTTT732850.035.9318963
AGAGCGG82550.035.914318
CGCCGTA346600.034.15050546-47
CACCGAG69950.033.5497743
CCGTATC355350.033.51563348-49
GTCGCCG364650.033.07541744-45
TCGGTGG431800.031.22016538-39
TCTCGGT408000.030.3421436-37
CCCAGAT313000.030.2940941
AGCGGCG56900.030.10981810-11
Produced by FastQC (version 0.11.5)