##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233048_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38114768
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776498049260066	34.0	31.0	34.0	30.0	34.0
2	31.97651920641364	34.0	31.0	34.0	30.0	34.0
3	31.937394765199674	34.0	31.0	34.0	30.0	34.0
4	35.216793920928495	37.0	35.0	37.0	33.0	37.0
5	35.20740729682521	37.0	35.0	37.0	33.0	37.0
6	35.164858303742	37.0	35.0	37.0	33.0	37.0
7	35.20952660658987	37.0	35.0	37.0	33.0	37.0
8	35.18136303492651	37.0	35.0	37.0	33.0	37.0
9	36.778349798692204	39.0	37.0	39.0	33.0	39.0
10-11	36.752341166027826	39.0	37.0	39.0	33.0	39.0
12-13	36.73714343479672	39.0	37.0	39.0	33.0	39.0
14-15	38.0605346069534	41.0	38.0	41.0	33.0	41.0
16-17	37.81429185138947	40.5	38.0	41.0	32.5	41.0
18-19	37.36008751253582	40.0	37.0	41.0	32.0	41.0
20-21	36.99119151138477	40.0	37.0	41.0	30.5	41.0
22-23	36.62562891632976	40.0	36.5	41.0	29.5	41.0
24-25	36.31330227433104	40.0	36.0	41.0	27.5	41.0
26-27	35.90490357963087	40.0	36.0	41.0	26.0	41.0
28-29	35.516836072569035	40.0	36.0	41.0	23.0	41.0
30-31	35.16212633119005	40.0	36.0	41.0	17.0	41.0
32-33	35.09583567450811	40.0	36.0	41.0	16.5	41.0
34-35	34.962456271542834	40.0	36.0	41.0	15.5	41.0
36-37	34.8051500536485	40.0	35.5	41.0	11.0	41.0
38-39	34.6871593839952	40.0	35.0	41.0	9.0	41.0
40-41	34.59401693065533	40.0	35.0	41.0	9.0	41.0
42-43	34.597019651280576	40.0	35.0	41.0	8.5	41.0
44-45	34.55921747969187	40.0	35.0	41.0	7.5	41.0
46-47	34.187031415224666	40.0	35.0	41.0	6.0	41.0
48-49	34.22899016465219	40.0	35.0	41.0	3.5	41.0
50-51	33.61947433341323	39.5	34.0	40.5	2.0	41.0
52-53	33.733076966387415	39.5	35.0	40.5	2.0	41.0
54-55	33.57320750319141	39.5	34.5	41.0	2.0	41.0
56-57	33.54741803754387	39.5	34.0	41.0	2.0	41.0
58-59	32.969521695632515	39.0	33.0	41.0	2.0	41.0
60-61	33.33463458310962	39.0	34.0	41.0	2.0	41.0
62-63	33.20990327161377	39.0	34.0	41.0	2.0	41.0
64-65	32.888486203038156	39.0	34.0	41.0	2.0	41.0
66-67	32.55240338862878	38.0	34.0	41.0	2.0	41.0
68-69	32.25014834145127	37.5	33.0	40.0	2.0	41.0
70-71	31.85499316170572	37.0	33.0	40.0	2.0	41.0
72-73	31.471541411455004	36.5	32.0	39.0	2.0	41.0
74-75	31.017416477518637	36.0	31.5	39.0	2.0	41.0
76-77	30.576151952440064	35.5	31.0	39.0	2.0	40.5
78-79	30.031201186899523	35.0	30.5	37.5	2.0	39.5
80-81	29.346720318486526	35.0	29.5	37.0	2.0	39.0
82-83	29.00107172369513	35.0	29.0	36.5	2.0	39.0
84-85	28.69168008578722	35.0	29.0	36.0	2.0	37.5
86-87	28.242150667163973	35.0	29.0	36.0	2.0	37.0
88-89	27.78040608826479	34.0	27.5	35.0	2.0	37.0
90-91	27.34877470590927	34.0	26.5	35.0	2.0	36.0
92-93	26.746776538164944	34.0	25.5	35.0	2.0	36.0
94-95	26.10590234997626	34.0	22.5	35.0	2.0	35.5
96-97	25.4627483105761	33.5	18.5	35.0	2.0	35.0
98-99	24.7021207632695	33.0	2.0	35.0	2.0	35.0
100-101	23.125045992671396	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4590986956433234
1101	2	0.38442389832469104
1101	3	0.4633011236376916
1101	4	0.48023936395800604
1101	5	0.441547286069337
1101	6	0.38665354678738595
1101	7	0.328231106283404
1101	8	0.28326198674614034
1101	9	0.3797615466386475
1101	10-11	0.42114934923627345
1101	12-13	0.44956154956735617
1101	14-15	0.5643231844904477
1101	16-17	0.5348427572053325
1101	18-19	0.5525562601072593
1101	20-21	0.634377118530729
1101	22-23	0.6537054469539143
1101	24-25	0.5747039435834154
1101	26-27	0.5551471422416157
1101	28-29	0.4683989495445431
1101	30-31	0.6218889956602638
1101	32-33	0.5955976660726208
1101	34-35	0.5421572091544036
1101	36-37	0.5825389163437578
1101	38-39	0.5440755336487015
1101	40-41	0.6292540356160359
1101	42-43	0.6024228647685845
1101	44-45	0.6121202451419236
1101	46-47	0.8864453200331255
1101	48-49	0.7122893624748698
1101	50-51	0.5080407568824725
1101	52-53	0.5340118492287687
1101	54-55	0.9567459446742035
1101	56-57	0.8416740826798446
1101	58-59	1.2240237353259005
1101	60-61	0.7164694887515637
1101	62-63	0.6053880112228356
1101	64-65	0.5922438496292699
1101	66-67	0.6059974123995175
1101	68-69	0.5922793434091815
1101	70-71	0.6026517231344997
1101	72-73	0.5499357617503016
1101	74-75	0.6048565325805093
1101	76-77	0.6640360235014384
1101	78-79	0.6949691984627329
1101	80-81	0.9534056784954217
1101	82-83	0.8229488584413431
1101	84-85	0.6467759749488096
1101	86-87	0.6953116587490555
1101	88-89	0.6915471789326268
1101	90-91	0.6236833085766165
1101	92-93	0.7592418000432701
1101	94-95	0.780514431011774
1101	96-97	0.6900625845966033
1101	98-99	0.6070477007606243
1101	100-101	0.7626812056616927
1102	1	0.30541584105022324
1102	2	0.27406307024365617
1102	3	0.2665511629520694
1102	4	0.30994618256005424
1102	5	0.29873868094048106
1102	6	0.332651361366473
1102	7	0.31707964035135205
1102	8	0.29200501936532675
1102	9	0.4074553520037014
1102	10-11	0.4061739834693583
1102	12-13	0.4039320378132132
1102	14-15	0.48556445011111293
1102	16-17	0.5160319971617966
1102	18-19	0.5387531940264552
1102	20-21	0.624492168932619
1102	22-23	0.6622503998439768
1102	24-25	0.5610020404060521
1102	26-27	0.4459654580567758
1102	28-29	0.4217054934257547
1102	30-31	0.4720239647186233
1102	32-33	0.5395879671305011
1102	34-35	0.5225905340340731
1102	36-37	0.5672527759067947
1102	38-39	0.553603260415656
1102	40-41	0.6283490410040358
1102	42-43	0.5715576873289905
1102	44-45	0.5845434649383492
1102	46-47	0.862435613052682
1102	48-49	0.6242629334224219
1102	50-51	0.4975059752262041
1102	52-53	0.5357879714285829
1102	54-55	0.9123084743837708
1102	56-57	0.8585614375263688
1102	58-59	1.2536428274638993
1102	60-61	0.6733105851163259
1102	62-63	0.5235187109120076
1102	64-65	0.5726587159088297
1102	66-67	0.5526121386189402
1102	68-69	0.5271725394479816
1102	70-71	0.5159927933970891
1102	72-73	0.5017904956915196
1102	74-75	0.5551170775873047
1102	76-77	0.5964980000921258
1102	78-79	0.6505300108538208
1102	80-81	0.9465763249726891
1102	82-83	0.859873745177989
1102	84-85	0.7233026213939908
1102	86-87	0.7325492523068853
1102	88-89	0.7819437388747303
1102	90-91	0.7394496733819338
1102	92-93	0.8080552735126574
1102	94-95	0.9119808484976346
1102	96-97	1.0322867119965196
1102	98-99	1.0339459750135482
1102	100-101	1.1446353827710176
1103	1	0.22315613863638717
1103	2	0.2309320307103846
1103	3	0.2725828821131415
1103	4	0.24605194715661582
1103	5	0.24738408979708026
1103	6	0.21437666000172584
1103	7	0.15437689264724241
1103	8	0.1923924638107266
1103	9	0.21413533889658964
1103	10-11	0.1999848863166136
1103	12-13	0.21008414958916433
1103	14-15	0.2339781189639467
1103	16-17	0.2708546755399013
1103	18-19	0.32648649448333344
1103	20-21	0.440940641320573
1103	22-23	0.46310658030233043
1103	24-25	0.3949496882290333
1103	26-27	0.36528399467417216
1103	28-29	0.35776143061706733
1103	30-31	0.4834365310787163
1103	32-33	0.4237160239632374
1103	34-35	0.3364617480547878
1103	36-37	0.39823419094962986
1103	38-39	0.41250427929629296
1103	40-41	0.4495410957990984
1103	42-43	0.4513236024281042
1103	44-45	0.4480933143880321
1103	46-47	0.6922757751714883
1103	48-49	0.539264103470039
1103	50-51	0.5078412689776926
1103	52-53	0.45297767421216406
1103	54-55	0.8359485334087324
1103	56-57	0.7529534383306142
1103	58-59	1.194910863673563
1103	60-61	0.6562475199192193
1103	62-63	0.4624452302724009
1103	64-65	0.45330108393054047
1103	66-67	0.42641751611319734
1103	68-69	0.4796314017716057
1103	70-71	0.48394723478121904
1103	72-73	0.46966935958763756
1103	74-75	0.4824568921384369
1103	76-77	0.5182598929089011
1103	78-79	0.6077911317178035
1103	80-81	0.8799125467001652
1103	82-83	0.7729672901140106
1103	84-85	0.6127257178793855
1103	86-87	0.6623159394977982
1103	88-89	0.6347349701309106
1103	90-91	0.6972521414801349
1103	92-93	0.7847437047962593
1103	94-95	0.852091241418055
1103	96-97	0.8861380854694758
1103	98-99	0.9120584819111954
1103	100-101	0.994260060703855
1104	1	0.09375860616320963
1104	2	0.128078966933856
1104	3	0.1627309194622626
1104	4	0.13506186661864916
1104	5	0.14239500987425657
1104	6	0.14971096274603468
1104	7	0.10833041516384156
1104	8	0.11376825306564342
1104	9	0.16390321906026628
1104	10-11	0.13796884164559486
1104	12-13	0.12487897334917619
1104	14-15	0.13256007744853804
1104	16-17	0.15924675211957862
1104	18-19	0.19251310850451375
1104	20-21	0.22853087236343583
1104	22-23	0.2925533140449019
1104	24-25	0.20083801475946927
1104	26-27	0.1507916214580476
1104	28-29	0.21218404854644035
1104	30-31	0.3251114758471587
1104	32-33	0.25747416361849673
1104	34-35	0.22705817278522744
1104	36-37	0.23909122577562414
1104	38-39	0.17923893165515636
1104	40-41	0.24651313084338256
1104	42-43	0.25594033112872694
1104	44-45	0.21771595005662192
1104	46-47	0.5040723708964734
1104	48-49	0.29321540184350425
1104	50-51	0.18273150695745954
1104	52-53	0.1751275847391227
1104	54-55	0.5692048830449963
1104	56-57	0.5409577766999689
1104	58-59	0.9515010544946634
1104	60-61	0.3784646281956441
1104	62-63	0.2695847537201317
1104	64-65	0.28634026504553844
1104	66-67	0.30397807441836733
1104	68-69	0.2907154972333714
1104	70-71	0.2895432025801341
1104	72-73	0.2889571200698029
1104	74-75	0.3091310575239845
1104	76-77	0.3335903623573415
1104	78-79	0.36112210235173947
1104	80-81	0.6128640349323895
1104	82-83	0.5506758689308526
1104	84-85	0.3705996470564443
1104	86-87	0.3696111386985592
1104	88-89	0.34590498505560063
1104	90-91	0.37488099463448776
1104	92-93	0.5096328779716188
1104	94-95	0.5444014663384849
1104	96-97	0.5933413018422584
1104	98-99	0.5445888823279468
1104	100-101	0.614179645583647
1105	1	-0.09348350989947107
1105	2	-0.06698274614199562
1105	3	-0.04331777115983115
1105	4	-0.005083348375819696
1105	5	-0.023995423184253184
1105	6	-0.04589412695423789
1105	7	-0.03097251511975685
1105	8	-0.010672011363212164
1105	9	-0.05717040498824133
1105	10-11	-0.06474310612357215
1105	12-13	-0.07593616586211738
1105	14-15	-0.10191275831247992
1105	16-17	-0.11180317978662657
1105	18-19	-0.06619385112425391
1105	20-21	-0.007850346652610085
1105	22-23	0.02090777464735538
1105	24-25	-0.06638218547563213
1105	26-27	-0.11165862891560607
1105	28-29	-0.052193767179154804
1105	30-31	0.07881286813955768
1105	32-33	-0.02833932694856145
1105	34-35	-0.028090105891500627
1105	36-37	-0.01220240033455866
1105	38-39	-0.0413000085266404
1105	40-41	-0.018708269629648555
1105	42-43	-0.04484127229354584
1105	44-45	-0.055678168190887334
1105	46-47	0.23205353048874855
1105	48-49	0.019316919342905692
1105	50-51	-0.13566538701305575
1105	52-53	-0.09149490102503677
1105	54-55	0.2374672408112346
1105	56-57	0.16308597029234306
1105	58-59	0.6014463902814455
1105	60-61	0.10208603788572645
1105	62-63	-0.075380696963947
1105	64-65	-0.04302383372053953
1105	66-67	0.009397595897432609
1105	68-69	0.029985709025261542
1105	70-71	0.02505718377656052
1105	72-73	-0.0019013843941557695
1105	74-75	0.08512375648739479
1105	76-77	0.09360107710676857
1105	78-79	0.1326958921565229
1105	80-81	0.3552941036121773
1105	82-83	0.27504834195258354
1105	84-85	0.11320215327529581
1105	86-87	0.15026956209711528
1105	88-89	0.09919242223890379
1105	90-91	0.16613845454682163
1105	92-93	0.24167052782364706
1105	94-95	0.3036337896072894
1105	96-97	0.3317554110303078
1105	98-99	0.3110836835636732
1105	100-101	0.3740781450891859
1106	1	-0.16599864342355275
1106	2	-0.16025755154081622
1106	3	-0.14092296488847111
1106	4	-0.12874446389785277
1106	5	-0.13937926479056273
1106	6	-0.11818071853269885
1106	7	-0.20215122995853818
1106	8	-0.124471454502455
1106	9	-0.13830463043002794
1106	10-11	-0.23263682886653214
1106	12-13	-0.2580147875604766
1106	14-15	-0.28194560412658376
1106	16-17	-0.30484983406761756
1106	18-19	-0.33115506757503965
1106	20-21	-0.2234949284080514
1106	22-23	-0.22337382849479326
1106	24-25	-0.34643971079695746
1106	26-27	-0.41516689248469874
1106	28-29	-0.3076462550816217
1106	30-31	-0.1979467808155917
1106	32-33	-0.28478936975918145
1106	34-35	-0.27213028856154153
1106	36-37	-0.2606883484081308
1106	38-39	-0.37300263280240387
1106	40-41	-0.2741080720915079
1106	42-43	-0.29625911149322803
1106	44-45	-0.37477604686477406
1106	46-47	-0.0519426466131776
1106	48-49	-0.22270556788217277
1106	50-51	-0.561721261378672
1106	52-53	-0.5353407349553052
1106	54-55	-0.17495673130606093
1106	56-57	-0.23491237371049323
1106	58-59	0.10491226967209144
1106	60-61	-0.42504771934058994
1106	62-63	-0.5336078690216439
1106	64-65	-0.5857183953231981
1106	66-67	-0.5078806845061123
1106	68-69	-0.4816232384652217
1106	70-71	-0.4806566184136152
1106	72-73	-0.4367983294568525
1106	74-75	-0.45586052038505187
1106	76-77	-0.3587044955406995
1106	78-79	-0.3289648706637358
1106	80-81	-0.038632002309153535
1106	82-83	-0.07272774805853643
1106	84-85	-0.2575079441086672
1106	86-87	-0.2871163139110351
1106	88-89	-0.3843413239421629
1106	90-91	-0.29269127910829695
1106	92-93	-0.25054777249407323
1106	94-95	-0.2337020162981034
1106	96-97	-0.17342328981975186
1106	98-99	-0.18608329579722493
1106	100-101	-0.12352327714717504
1107	1	-0.5164756538273494
1107	2	-0.3876188584628224
1107	3	-0.4592826653302211
1107	4	-0.5368291037200024
1107	5	-0.4259711939310975
1107	6	-0.40312827305189813
1107	7	-0.370312609719619
1107	8	-0.31165438905265574
1107	9	-0.4605299882183189
1107	10-11	-0.49165923417216106
1107	12-13	-0.5007026669643224
1107	14-15	-0.6819504251173285
1107	16-17	-0.7218932412198669
1107	18-19	-0.6495016364945769
1107	20-21	-0.6252390662560074
1107	22-23	-0.6026100529459413
1107	24-25	-0.6819995219104698
1107	26-27	-0.764810506139078
1107	28-29	-0.781052301541969
1107	30-31	-0.7122227879023839
1107	32-33	-0.6984347662730244
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2308	68-69	-1.6240117967176104
2308	70-71	-1.6285623358337276
2308	72-73	-1.62078911639232
2308	74-75	-1.6418689216087543
2308	76-77	-1.753437227813322
2308	78-79	-1.7125905606302467
2308	80-81	-1.7673612804026426
2308	82-83	-1.8382995264002524
2308	84-85	-2.038911520277324
2308	86-87	-2.223760051278468
2308	88-89	-2.567728281515709
2308	90-91	-2.78988815939816
2308	92-93	-3.0844562045863206
2308	94-95	-3.3521941975940877
2308	96-97	-3.74499495022787
2308	98-99	-4.1862048843768775
2308	100-101	-4.5152091322432355
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	407522.0
3	94230.0
4	73920.0
5	80358.0
6	119042.0
7	192714.0
8	307058.0
9	481604.0
10	629202.0
11	578989.0
12	398167.0
13	303176.0
14	251256.0
15	221407.0
16	204637.0
17	199476.0
18	201157.0
19	209392.0
20	225574.0
21	239206.0
22	263008.0
23	295389.0
24	346094.0
25	351816.0
26	352138.0
27	391981.0
28	438351.0
29	501847.0
30	582772.0
31	697229.0
32	852210.0
33	1079132.0
34	1440125.0
35	2132414.0
36	3246517.0
37	6204341.0
38	9784066.0
39	3728006.0
40	9245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.795165017818782	16.58591408027098	9.606054681044581	42.01286622086566
2	15.184274722009466	17.977967503541855	52.879060966779456	13.958696807669222
3	18.367538233907936	22.303191142013198	36.78360719020966	22.545663433869205
4	20.427267113867106	27.370235569443224	33.596576752447056	18.605920564242616
5	20.392055668329455	30.296875815504322	35.572264672261525	13.738803843904696
6	15.651012549285484	35.18598582609195	35.02072711833946	14.142274506283107
7	12.595834799046921	19.55079845662265	53.47534601837359	14.378020725956834
8	13.138865314403148	22.802857417803974	46.31943317634114	17.738844091451732
9	14.907567546166275	22.95913460818021	44.36394196544505	17.769355880208472
10-11	17.358549823915467	32.1061421014959	35.502363794609856	15.032944279978771
12-13	16.69302956765456	25.817428265576304	40.87681196417029	16.612730202598847
14-15	17.117076170708657	28.22706434897359	39.2582405218818	15.397618958435947
16-17	18.97738292780881	28.18309844910374	37.231289838628875	15.608228784458579
18-19	18.97109774142518	28.34433207788044	36.77666665189081	15.907903528803574
20-21	19.2216417625594	29.59258576562692	34.843703762610275	16.342068709203406
22-23	19.192341293286148	29.15093848359664	35.36918680630605	16.28753341681117
24-25	18.407388109785153	30.17629544440207	34.411886036456295	17.00443040935648
26-27	17.864470173458137	31.270638526188993	33.96152546594348	16.90336583440939
28-29	18.266457108809483	30.61183885310384	33.75609817414122	17.365605863945454
30-31	18.880737424638617	30.60867871326713	33.194971580667385	17.31561228142687
32-33	18.0158166721338	30.256321825334314	33.73523820266929	17.992623299862593
34-35	18.535499293833528	31.60650961771989	32.453583088150076	17.404408000296513
36-37	18.607321926740944	31.309394276960045	32.84544453186798	17.237839264431027
38-39	18.82786680366427	30.345316781922165	33.43147202872186	17.39534438569169
40-41	19.598166928566467	30.92677558368807	32.327363024807326	17.147694462938137
42-43	18.481458065065723	30.612417218000594	33.509923879094636	17.396200837839054
44-45	19.209967393753622	29.762024194848575	33.357522514795505	17.670485896602294
46-47	18.171309215236285	30.98311377588879	32.760175311916896	18.08540169695803
48-49	17.376650408151573	30.71044747676276	33.10810542111571	18.804796693969955
50-51	17.82858329786888	30.566178335260254	33.621337900089664	17.983900466781204
52-53	17.7295529366232	30.934619599483838	33.209522290038485	18.12630517385448
54-55	18.14556412970527	31.65267604724516	31.762391503567823	18.439368319481744
56-57	18.896794275887398	31.40875330639745	32.42314201896382	17.27131039875132
58-59	18.34728005644442	32.12241755409737	32.2362339814993	17.29406840795891
60-61	18.616023975026888	33.25551868189883	30.672200341712426	17.45625700136186
62-63	18.188921873858767	33.250383428060246	31.17201778955972	17.388676908521262
64-65	18.539339653015162	31.970195560870994	31.855796946286972	17.634667839826864
66-67	18.0842206776236	33.19095656655875	30.921672374699146	17.80315038111851
68-69	18.06764297907235	32.62748663167335	31.59970960242416	17.70516078683014
70-71	17.790674657430145	32.13338035018274	31.642334834331585	18.43361015805553
72-73	17.842300375850833	32.33605409896874	31.441236067199434	18.380409457981
74-75	18.369112481453456	32.22113409876937	31.06466945598081	18.34508396379637
76-77	18.59396033244555	32.25407041391777	30.948786671842036	18.203182581794632
78-79	18.626751930679024	32.7952600977511	30.946603960364193	17.631384011205686
80-81	18.958509980019908	33.1915064720956	30.251923076140226	17.59806047174427
82-83	18.715522519874376	32.17071264998951	31.168679026794134	17.945085803341975
84-85	18.802201234391696	32.44275118991447	30.847491213249484	17.907556362444353
86-87	18.25536123920734	32.818508844854115	31.19950154199084	17.726628373947698
88-89	18.3537861259411	32.5651332388454	30.96140228821877	18.119678346994732
90-91	18.125608914478416	32.989626074390515	30.719176728911325	18.165588282219744
92-93	18.568246869206458	32.0947504592221	31.06424216466268	18.27276050690876
94-95	18.666021161545448	33.205796387346936	29.988331135722397	18.13985131538522
96-97	18.955272796109472	33.18659316695041	30.141281261450835	17.71685277548928
98-99	19.522858787278036	32.550055220153446	30.211426854169975	17.71565913839854
100-101	19.884380999453008	33.760678546118584	28.64068341119002	17.714257043238387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93980.0
1	90795.5
2	74593.5
3	66684.5
4	80021.5
5	97521.0
6	107514.0
7	112373.5
8	120036.0
9	127377.0
10	134379.5
11	140821.0
12	148411.0
13	154847.0
14	166401.0
15	177574.5
16	182778.5
17	191984.0
18	207044.5
19	227955.5
20	257827.5
21	298210.0
22	348361.0
23	401450.5
24	464941.0
25	563834.0
26	664630.0
27	770220.0
28	873545.0
29	965844.0
30	1059586.5
31	1122083.5
32	1214415.0
33	1384204.0
34	1534251.0
35	1882132.5
36	1961054.5
37	1700059.5
38	1642719.5
39	1557698.0
40	1534527.5
41	1549286.0
42	1366020.5
43	1160946.0
44	1047410.5
45	958475.0
46	893200.5
47	821012.0
48	764186.5
49	709307.0
50	616587.0
51	520679.0
52	456445.5
53	410888.0
54	362233.5
55	311693.0
56	271617.0
57	234776.5
58	201069.0
59	163630.0
60	122468.5
61	88044.0
62	66614.5
63	50798.5
64	35563.5
65	23623.5
66	15473.5
67	10120.5
68	6505.5
69	4203.0
70	2885.5
71	2118.0
72	1748.0
73	1630.0
74	1381.5
75	970.0
76	796.5
77	683.5
78	564.5
79	422.0
80	292.0
81	197.0
82	135.5
83	96.5
84	69.5
85	48.5
86	38.0
87	28.5
88	24.0
89	19.5
90	11.5
91	8.0
92	7.0
93	5.5
94	3.0
95	3.0
96	2.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13968863722324112
2	0.15331590107015738
3	0.15879146896552013
4	0.11855509654420564
5	0.11544344176514468
6	0.1745937427718306
7	0.19153205917454358
8	0.18640806104342547
9	0.20242547455621404
10-11	0.1969945087951211
12-13	0.20393669981147466
14-15	0.22142204827273249
16-17	0.25392650953562146
18-19	0.2684615579976769
20-21	0.26505474203594787
22-23	0.2914014851146411
24-25	0.28782019609826826
26-27	0.27508235128179187
28-29	0.22931269055605952
30-31	0.23783956916647114
32-33	0.28958985136679827
34-35	0.29582103188979136
36-37	0.30061576132379975
38-39	0.29809311708259645
40-41	0.2946915484307815
42-43	0.30419311485773703
44-45	0.3089458658124326
46-47	0.3022017607453363
48-49	0.048066670640629375
50-51	0.07479646734305191
52-53	0.09138583763647729
54-55	0.10830710028196945
56-57	0.11313200174798389
58-59	0.09881602847484208
60-61	0.10971600299390515
62-63	0.10278693025233684
64-65	0.10152232856303887
66-67	0.1085524120204536
68-69	0.10512985412898224
70-71	0.09503009437181933
72-73	0.09435843870281461
74-75	0.08095025004481203
76-77	0.09032456920635068
78-79	0.07543139184265794
80-81	0.10263082278239238
82-83	0.1258869003216811
84-85	0.12912842602111602
86-87	0.13164844660736227
88-89	0.12455145994854278
90-91	0.10856553029523885
92-93	0.10294566137723835
94-95	0.10816148743185319
96-97	0.1201043647963435
98-99	0.12012797769095697
100-101	0.12461180401255492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.8114768E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.18330726708213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48686685839637	22.1201948510075
2	9.371942230970031	5.282646551695433
3	3.483235323998758	2.9450727425943417
4	1.8400606285501468	2.074359763379563
5	1.168150004387298	1.6461165253845282
6	0.838139886654078	1.4172932375021565
7	0.6063554653936731	1.1962371675985135
8	0.4714624252973195	1.062989631763049
9	0.37915625296759625	0.9617289461659159
>10	2.7382004447357113	15.267646731080303
>50	0.330566573367299	6.495425794025948
>100	0.24153855744225164	13.494506533723058
>500	0.024535493467164524	4.763736478320994
>1k	0.017092671775644788	9.57780587819958
>5k	0.0015804543642244737	3.0688001244880994
>10k+	0.0011167282326380082	8.62543904307123
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT	259336	0.6804081819414459	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	114841	0.3013031589225468	Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94657	0.24834730726945525	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94360	0.24756808174721148	No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA	91042	0.23886279459972048	No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT	88374	0.23186288317431186	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	87113	0.22855445427347218	No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG	82050	0.21527088922592943	No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG	80559	0.2113590196849683	No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT	64335	0.16879284166179365	No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC	63601	0.16686707892331917	No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT	61954	0.16254591920905828	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	61035	0.1601347803035296	No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA	59586	0.15633310427076455	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	57096	0.14980020342771075	Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	54901	0.1440412807969866	No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT	48162	0.12636047004142856	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	44646	0.11713569921244174	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	43062	0.11297982976047499	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	39285	0.1030702849876982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.19698663783024994	0.0	0.0	2.8860204527546908E-5	0.0
2	0.19850573405038174	0.0	2.623654957049719E-6	3.410751444164634E-5	0.0
3	0.19859493831892142	0.0	2.623654957049719E-6	4.1978479312795505E-5	0.0
4	0.2893786471427558	0.0	2.623654957049719E-6	4.460213426984522E-5	0.0
5	0.2904333564354898	0.0	2.623654957049719E-6	5.7720409055093817E-5	0.0
6	0.2910210551458689	0.0	2.623654957049719E-6	6.296771896919326E-5	0.0
7	0.29588268778128207	0.0	2.623654957049719E-6	7.870964871149157E-5	0.0
8	0.3613297606848873	0.0	2.623654957049719E-6	8.133330366854128E-5	0.0
9	0.36178102933749984	0.0	2.623654957049719E-6	9.70752334108396E-5	0.0
10-11	0.37458315370042394	0.0	2.623654957049719E-6	9.70752334108396E-5	0.0
12-13	0.3821300971843774	0.0	2.623654957049719E-6	1.0232254332493904E-4	0.0
14-15	0.38656276223431296	0.0	2.623654957049719E-6	1.1412899063166276E-4	0.0
16-17	0.38940286872531926	0.0	2.623654957049719E-6	1.259354379383865E-4	0.0
18-19	0.3919845452030562	1.3118274785248595E-6	2.623654957049719E-6	1.4036554020215996E-4	0.0
20-21	0.3950476623654118	2.623654957049719E-6	2.623654957049719E-6	1.6004295238003285E-4	0.0
22-23	0.3977382205238662	2.623654957049719E-6	2.623654957049719E-6	1.6922574472970687E-4	0.0
24-25	0.39998144551214376	5.247309914099438E-6	2.623654957049719E-6	1.9152681186462947E-4	0.0
26-27	0.40258673488449415	5.247309914099438E-6	2.623654957049719E-6	2.7023646057612106E-4	0.0
28-29	0.40529697045512647	5.247309914099438E-6	2.623654957049719E-6	3.253332146741652E-4	0.0
30-31	0.40845453919593583	1.3118274785248594E-5	2.623654957049719E-6	3.3058052458826456E-4	0.0
32-33	0.4116462154511868	1.4430102263773454E-5	2.623654957049719E-6	3.371396619808889E-4	0.0
34-35	0.41508976258231456	2.361289461344747E-5	2.623654957049719E-6	3.4501062685203803E-4	0.0
36-37	0.41855692260805577	2.6236549570497187E-5	2.623654957049719E-6	3.843654512077838E-4	0.0
38-39	0.422739028609593	2.8860204527546908E-5	2.623654957049719E-6	3.948600710359827E-4	0.0
40-41	0.4273435430592153	3.148385948459662E-5	2.623654957049719E-6	4.315912404346788E-4	0.0
42-43	0.43188115430743274	3.6731169398696064E-5	2.623654957049719E-6	4.6176327244075053E-4	0.0
44-45	0.43770698013956166	3.6731169398696064E-5	2.623654957049719E-6	4.8668799453272286E-4	0.0
46-47	0.45795503727059284	3.6731169398696064E-5	2.623654957049719E-6	6.5329008430538E-4	2.623654957049719E-6
48-49	0.4660529482955268	3.6731169398696064E-5	2.623654957049719E-6	6.847739437899766E-4	2.623654957049719E-6
50-51	0.47280492432749427	3.6731169398696064E-5	5.247309914099438E-6	6.965803910967004E-4	2.623654957049719E-6
52-53	0.48054470645079095	3.804299687722092E-5	5.247309914099438E-6	7.005158735322749E-4	2.623654957049719E-6
54-55	0.4889312195210004	3.9354824355745785E-5	5.247309914099438E-6	7.123223208389986E-4	2.623654957049719E-6
56-57	0.4982032161392141	3.9354824355745785E-5	5.247309914099438E-6	7.293760780598218E-4	2.623654957049719E-6
58-59	0.5090808895911422	4.1978479312795505E-5	5.247309914099438E-6	7.438061803235953E-4	2.623654957049719E-6
60-61	0.5217964333404836	4.1978479312795505E-5	5.247309914099438E-6	7.55612627630319E-4	2.623654957049719E-6
62-63	0.5353344929188603	4.1978479312795505E-5	5.247309914099438E-6	7.870964871149156E-4	2.623654957049719E-6
64-65	0.5503090035862215	4.1978479312795505E-5	5.247309914099438E-6	8.159566916424625E-4	2.623654957049719E-6
66-67	0.5670426749022845	4.460213426984522E-5	5.247309914099438E-6	8.316986213847609E-4	2.623654957049719E-6
68-69	0.5865456140255136	4.460213426984522E-5	5.247309914099438E-6	8.421932412129597E-4	2.623654957049719E-6
70-71	0.6095524443438827	4.722578922689494E-5	5.247309914099438E-6	8.526878610411586E-4	2.623654957049719E-6
72-73	0.6348890802641118	4.722578922689494E-5	5.247309914099438E-6	8.553115159982083E-4	2.623654957049719E-6
74-75	0.6647646392600369	4.722578922689494E-5	5.247309914099438E-6	8.95978167832479E-4	2.623654957049719E-6
76-77	0.6993221105268174	4.984944418394466E-5	5.247309914099438E-6	0.0010192899508138157	2.623654957049719E-6
78-79	0.7406840309247061	5.2473099140994375E-5	5.247309914099438E-6	0.001062580257605136	2.623654957049719E-6
80-81	0.7900074322897623	5.2473099140994375E-5	6.559137392624298E-6	0.0011045587369179317	2.623654957049719E-6
82-83	0.8500379695345384	5.2473099140994375E-5	7.870964871149157E-6	0.0011504726986663017	2.623654957049719E-6
84-85	0.9228325881453614	5.2473099140994375E-5	7.870964871149157E-6	0.0011622791459730253	2.623654957049719E-6
86-87	1.0110346204914589	5.2473099140994375E-5	7.870964871149157E-6	0.0011688382833656497	2.623654957049719E-6
88-89	1.1203728171715488	5.2473099140994375E-5	7.870964871149157E-6	0.0011727737658012243	2.623654957049719E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	40710	0.0	64.43726	1
GAGCGGC	6150	0.0	46.8994	9
GCGTGTT	55875	0.0	46.762386	2
ACCACCG	5320	0.0	44.29063	1
CCACCGA	5695	0.0	41.708805	2
GTTTTTT	574770	0.0	40.93039	1
ATCGGGA	6240	0.0	40.502193	2
ACCGAGA	6415	0.0	37.101254	4
CCCGAAA	7385	0.0	36.923527	1
CCGAGAT	11295	0.0	35.960163	1
CGTGTTT	73285	0.0	35.931896	3
AGAGCGG	8255	0.0	35.91431	8
CGCCGTA	34660	0.0	34.150505	46-47
CACCGAG	6995	0.0	33.549774	3
CCGTATC	35535	0.0	33.515633	48-49
GTCGCCG	36465	0.0	33.075417	44-45
TCGGTGG	43180	0.0	31.220165	38-39
TCTCGGT	40800	0.0	30.34214	36-37
CCCAGAT	31300	0.0	30.294094	1
AGCGGCG	5690	0.0	30.109818	10-11
>>END_MODULE