FastQCFastQC Report
Fri 27 May 2016
SRR1233048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38114768
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2879030.7553581330994852TruSeq Adapter, Index 3 (100% over 50bp)
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT2473560.6489767955559902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA913720.23972860073554694No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA907300.238044214253121No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG861060.2259124337317231No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT858330.22519617592844854No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT821680.2155804805108613No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG771300.20236250683724483No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT704380.18480500786466808No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG673540.17671365597712677TruSeq Adapter, Index 3 (100% over 49bp)
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC665860.17469868897011256No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT640450.16803198172424924No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT626230.16430114437532456No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT623290.1635297898179519No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA604370.15856583463921387No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA560400.14702962379306625No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT499950.1311696295777007No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT462310.12129419231936556No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC437670.11482950650519505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT414000.068.196211
CGGTGGT55250.059.611951
GCGTGTT570650.049.314112
GTTTTTT5466700.041.7276151
CGGAGAT57850.040.47821
GAGCACA930050.039.5723659
CGGAAGA947500.038.8886834
CCCGAAA66150.038.6370471
TCGGAAG956650.038.467083
ATCGGAA979300.038.072082
AGAGCAC967450.038.0572978
CGTGTTT751100.037.7826843
GATCGGA1000200.037.2878271
TATGCCG589450.036.60769346-47
TGCCGTC597200.035.76667448-49
CCGTCTT604850.035.60862450-51
CTCGTAT608150.035.05252542-43
CCGAGAT61500.034.748071
ATCTCGT613850.033.72873740-41
GTGGTCG25700.033.4501573
Produced by FastQC (version 0.11.5)