FastQCFastQC Report
Fri 27 May 2016
SRR1233047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47295056
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT5801361.2266313840499523No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT5303631.1213920541715818No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT3076220.6504316222820414No Hit
GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA2787680.5894231312465302No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT2736310.5785615308289307No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA2653010.5609486962019878No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA1957100.4138064663672245No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG1862690.39384454899471943No Hit
GTCGTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTA1843100.38970246699781896No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT1806180.38189615421958695No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1757690.3716434969439512No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC1524530.32234447507578806No Hit
CTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTA1443610.3052348642953293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1442780.30505937026483276No Hit
ATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGA1420390.3003252602132451No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC1377830.2913264337820004No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1328830.2809659428249752No Hit
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT1317920.2786591477976049No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA1242410.2626934197942381No Hit
CGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTC1167430.24683975424407995No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC1155910.24440398167622426No Hit
GGAGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGAT1141570.2413719522818622No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG1095250.23157811674860898No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT1033010.2184181788472774No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA1014590.21452348000179977No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC1002870.2120454197157521No Hit
AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA986030.20848479384399082No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT925270.19563778505727955No Hit
GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC911380.19270090302884937No Hit
CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT883770.1868630835324521No Hit
GTCGTATTATCTCTCTATAGTGAGTCGTATTATCTCTCTATAGTGAGTCG858770.18157711875845967No Hit
ATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATC839020.17740120658700564No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT820860.1735614817751775No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT820380.17345999125151684No Hit
GGGAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAAT810810.17143652393603254No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA718870.15199685988319794No Hit
ATACGACTCACTATAGGAGAGATAATACGACTCACTATAGGAGAGATAAT713980.150962925173405No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG705290.14912552381796523No Hit
ATACGACTCACTATAGAGAGATAATACGACTCACTATAGAGAGATAATAC681440.14408271342357645No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT679650.1437042383457586No Hit
GTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTATTA648350.13708621044872005No Hit
AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA647990.13701009255597454No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC605810.1280916127892945No Hit
GAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAAT599650.12678915106898275No Hit
GTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTA598620.12657136932029428No Hit
GAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAA594400.12567909846644434No Hit
CTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATA585130.12371906272824797No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA575080.121594104889103No Hit
CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT554610.11726595693215798No Hit
CTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTCACT553910.11711794991848619No Hit
CGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGACTC528680.11178335426857303No Hit
AGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAA515530.10900293679745299No Hit
GATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGA477340.10092809700870213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT657250.059.1959761
CCCGAAA109100.050.8515241
CGGTCGT7350.050.407261
GCGTGTT818600.047.817382
CCGAAAC140900.038.9020422
CGCCGTA81200.034.8199646-47
CGAAACC158550.034.541463
CGCGCGT9850.033.2735181
TCGTCTA120800.033.22963744-45
CGGCCTT45150.033.033741
CGAGCTA122750.032.9649812-13
CGTGTTT1203500.032.899493
ACTCGTC122500.032.6309142-43
CTCGTAT54650.032.2455371
CTAACGA115400.032.2393994-95
GTCGCCG90550.032.1665944-45
CCAAACG174250.031.4076258
GTTTTTT6308000.030.8706841
TCGGTGG110300.030.11666738-39
CGAGTCG49800.029.4723641
Produced by FastQC (version 0.11.5)