##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233047_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47295056 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8891841675798 33.0 31.0 34.0 30.0 34.0 2 32.13026767533587 33.0 31.0 34.0 30.0 34.0 3 32.16655681727071 34.0 31.0 34.0 30.0 34.0 4 35.63665840674763 37.0 35.0 37.0 33.0 37.0 5 35.52493575649852 37.0 35.0 37.0 33.0 37.0 6 35.59358766802179 37.0 35.0 37.0 33.0 37.0 7 35.70360811075052 37.0 35.0 37.0 35.0 37.0 8 35.70664536267808 37.0 35.0 37.0 33.0 37.0 9 37.31129279136491 39.0 37.0 39.0 34.0 39.0 10-11 37.21648474208382 39.0 37.0 39.0 33.5 39.0 12-13 37.19985639725218 39.0 37.0 39.0 34.0 39.0 14-15 38.47633527487524 40.0 38.0 41.0 34.0 41.0 16-17 38.24836729234447 40.0 38.0 41.0 33.0 41.0 18-19 37.88184614899282 40.0 37.0 41.0 33.0 41.0 20-21 37.655436532309 40.0 37.0 41.0 33.0 41.0 22-23 37.38872178309716 40.0 37.0 41.0 32.0 41.0 24-25 37.150624475420855 40.0 37.0 41.0 32.0 41.0 26-27 36.78872207065365 40.0 37.0 41.0 31.0 41.0 28-29 36.485994582605 40.0 37.0 41.0 30.0 41.0 30-31 36.22372156616116 40.0 36.5 41.0 29.0 41.0 32-33 35.99774553602389 40.0 36.0 41.0 27.0 41.0 34-35 35.76321181435962 40.0 36.0 41.0 26.0 41.0 36-37 35.59535698615095 40.0 36.0 41.0 25.0 41.0 38-39 35.39469838031273 40.0 35.0 41.0 25.0 41.0 40-41 35.30391127985978 39.5 35.0 41.0 24.0 41.0 42-43 35.33361446913183 40.0 35.0 41.0 24.0 41.0 44-45 35.211015914644435 40.0 35.0 41.0 24.0 41.0 46-47 35.091720210670644 39.5 35.0 41.0 23.5 41.0 48-49 34.988025429127305 39.0 35.0 41.0 23.0 41.0 50-51 34.55323705505286 38.5 35.0 40.5 22.0 41.0 52-53 34.549585193429095 39.0 35.0 40.0 21.0 41.0 54-55 34.661328480084684 39.0 35.0 41.0 20.0 41.0 56-57 34.5141682462539 39.0 35.0 41.0 18.5 41.0 58-59 34.28299852314373 39.0 35.0 41.0 16.5 41.0 60-61 34.080071181224525 39.0 34.0 40.5 13.5 41.0 62-63 33.74010019144496 38.0 34.0 40.0 9.5 41.0 64-65 33.44700187055493 38.0 34.0 40.0 8.0 41.0 66-67 33.17406671428827 38.0 33.5 40.0 7.0 41.0 68-69 32.78189587089187 37.0 33.0 39.5 3.5 41.0 70-71 32.39173599879024 37.0 32.5 39.0 2.0 41.0 72-73 31.989445302697177 36.0 32.0 39.0 2.0 40.5 74-75 31.557348520741787 36.0 31.5 38.5 2.0 40.0 76-77 31.105199399700467 35.0 31.0 38.0 2.0 40.0 78-79 30.57446648334659 35.0 31.0 37.0 2.0 39.0 80-81 30.15193015100775 35.0 30.0 36.5 2.0 39.0 82-83 29.710462685571194 35.0 30.0 36.0 2.0 39.0 84-85 29.309692296378717 34.5 29.5 36.0 2.0 37.0 86-87 28.830099831153596 34.0 29.0 35.0 2.0 37.0 88-89 28.48414812110594 34.0 29.0 35.0 2.0 36.5 90-91 28.066980056012618 34.0 29.0 35.0 2.0 36.0 92-93 27.636495567316803 34.0 27.5 35.0 2.0 36.0 94-95 27.222693699738933 34.0 26.5 35.0 2.0 35.5 96-97 26.716529989942288 34.0 25.5 35.0 2.0 35.0 98-99 26.115700412745046 34.0 24.5 35.0 2.0 35.0 100-101 23.652856061741424 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32353773472072334 1101 2 0.2690000031045372 1101 3 0.29744539585353635 1101 4 0.3045596192782085 1101 5 0.30018972568015556 1101 6 0.3102610125572838 1101 7 0.26344472744615643 1101 8 0.26194167896355935 1101 9 0.38096622501883104 1101 10-11 0.41187884320748225 1101 12-13 0.3807668342018573 1101 14-15 0.46200173224186614 1101 16-17 0.46184618011226064 1101 18-19 0.4935522659398899 1101 20-21 0.4464059367601365 1101 22-23 0.48173647469203473 1101 24-25 0.47414479538804954 1101 26-27 0.48824481537002384 1101 28-29 0.3932778260965293 1101 30-31 0.4655172106586818 1101 32-33 0.419272981314208 1101 34-35 0.432873066807808 1101 36-37 0.42722752676097997 1101 38-39 0.41246209676238976 1101 40-41 0.46601616900196063 1101 42-43 0.4666249899579995 1101 44-45 0.47669312980850975 1101 46-47 0.3971946405919269 1101 48-49 0.42638153933474854 1101 50-51 0.44600870615097676 1101 52-53 0.4554770805706525 1101 54-55 0.48941373744011685 1101 56-57 0.48037254769149484 1101 58-59 0.5108145221951119 1101 60-61 0.47242821899181564 1101 62-63 0.4710150107713247 1101 64-65 0.4618922224672346 1101 66-67 0.41764281620350374 1101 68-69 0.41403718025478753 1101 70-71 0.35194204572988497 1101 72-73 0.38636149800556296 1101 74-75 0.3551324027124032 1101 76-77 0.29022238891367635 1101 78-79 0.3896641510484393 1101 80-81 0.34281021291940306 1101 82-83 0.34892298338213124 1101 84-85 0.34806537648388414 1101 86-87 0.35148898441391907 1101 88-89 0.23425192394067906 1101 90-91 0.19601870999080973 1101 92-93 0.12753120911269278 1101 94-95 0.1719507322358389 1101 96-97 0.09683408146091566 1101 98-99 0.09963786804066999 1101 100-101 0.27702624098589723 1102 1 0.24497322103233188 1102 2 0.1964002180868647 1102 3 0.24293437816000107 1102 4 0.2242991687316831 1102 5 0.206789900149829 1102 6 0.24855514045307814 1102 7 0.19633152532479414 1102 8 0.186373256889226 1102 9 0.31351085977495075 1102 10-11 0.3007381949688508 1102 12-13 0.31076826902502575 1102 14-15 0.3618801219362169 1102 16-17 0.36223396819973175 1102 18-19 0.3543703382007166 1102 20-21 0.303422396834371 1102 22-23 0.314562401102485 1102 24-25 0.3206383287365284 1102 26-27 0.29302765367931727 1102 28-29 0.22877252960960703 1102 30-31 0.24965531901918325 1102 32-33 0.26637641096355935 1102 34-35 0.25111696986440535 1102 36-37 0.2631955772281742 1102 38-39 0.2260797079506247 1102 40-41 0.31163889129555145 1102 42-43 0.21914784916265972 1102 44-45 0.19363652844639745 1102 46-47 0.13945178622225995 1102 48-49 0.1856552354693477 1102 50-51 0.1887613721790018 1102 52-53 0.1721032627172292 1102 54-55 0.20676191295395085 1102 56-57 0.18216495530666776 1102 58-59 0.20264871451326627 1102 60-61 0.14525058245904887 1102 62-63 0.10240350361016937 1102 64-65 0.14438553516644248 1102 66-67 0.17973855628589064 1102 68-69 0.19505558517754906 1102 70-71 0.16639072698875168 1102 72-73 0.08728381800609242 1102 74-75 0.08911800986867746 1102 76-77 0.10088263665578978 1102 78-79 0.15100246124876904 1102 80-81 0.12719055921819233 1102 82-83 0.03695236229059162 1102 84-85 0.059322390086368415 1102 86-87 0.04429085040746372 1102 88-89 -0.024576832326875575 1102 90-91 -0.05640720219198769 1102 92-93 -0.045414452687431606 1102 94-95 0.0014789105349208853 1102 96-97 -0.018255025277266412 1102 98-99 -0.1903044737715831 1102 100-101 0.007822163917808211 1103 1 0.19260164686351544 1103 2 0.1689701195700195 1103 3 0.22967747409754224 1103 4 0.25827343233500244 1103 5 0.2392834448800869 1103 6 0.24043608838938013 1103 7 0.25121013022859984 1103 8 0.23348796068135158 1103 9 0.25310187007203666 1103 10-11 0.3121247036305377 1103 12-13 0.29691105762669423 1103 14-15 0.3472600752065702 1103 16-17 0.3095165382462497 1103 18-19 0.3063462425817889 1103 20-21 0.317831018567027 1103 22-23 0.38072602692655977 1103 24-25 0.38774353150380847 1103 26-27 0.41403339031928255 1103 28-29 0.41891950951652035 1103 30-31 0.4125069177598917 1103 32-33 0.38920717014258344 1103 34-35 0.41249566529871373 1103 36-37 0.41665865458240603 1103 38-39 0.3661372170469548 1103 40-41 0.44227546697788966 1103 42-43 0.4233947791959096 1103 44-45 0.426814702546622 1103 46-47 0.4472845349671175 1103 48-49 0.47151073591123094 1103 50-51 0.46446568667123245 1103 52-53 0.5221756239634345 1103 54-55 0.5070586764063876 1103 56-57 0.5117812322710762 1103 58-59 0.526972277335588 1103 60-61 0.5104068072385815 1103 62-63 0.42560855263933917 1103 64-65 0.4237422866604277 1103 66-67 0.44531727782667474 1103 68-69 0.40676691590117997 1103 70-71 0.4325939102005094 1103 72-73 0.3611166867565032 1103 74-75 0.42524295553867475 1103 76-77 0.35775549883747715 1103 78-79 0.41874513915525213 1103 80-81 0.4171037388227674 1103 82-83 0.39754535817671766 1103 84-85 0.36801175628391647 1103 86-87 0.32996076330635304 1103 88-89 0.26223360474132207 1103 90-91 0.30094848703057764 1103 92-93 0.3458712127071273 1103 94-95 0.38025392631342925 1103 96-97 0.37555331485170385 1103 98-99 0.3287400714494595 1103 100-101 0.21343045641884117 1104 1 0.24248943389005007 1104 2 0.17841110965147777 1104 3 0.20122012297108682 1104 4 0.2502182000923767 1104 5 0.2182099338382244 1104 6 0.21061949776620992 1104 7 0.21442797959699078 1104 8 0.21755320619651286 1104 9 0.30534734706886013 1104 10-11 0.324837169597302 1104 12-13 0.31830871865249577 1104 14-15 0.3448280269734738 1104 16-17 0.31075870208357514 1104 18-19 0.3389674819623778 1104 20-21 0.3747597669294862 1104 22-23 0.4216864923991892 1104 24-25 0.380785261812278 1104 26-27 0.3958210781024789 1104 28-29 0.4312962200126904 1104 30-31 0.3789731099917404 1104 32-33 0.3960637911386087 1104 34-35 0.43672598378163485 1104 36-37 0.38800460966663053 1104 38-39 0.3680386497352899 1104 40-41 0.4728599295308271 1104 42-43 0.3563834891528046 1104 44-45 0.39870141428853856 1104 46-47 0.33989351221286057 1104 48-49 0.4256060009023699 1104 50-51 0.40609255540885414 1104 52-53 0.465659372421662 1104 54-55 0.5036151969267024 1104 56-57 0.4334243197366163 1104 58-59 0.46443389366863386 1104 60-61 0.4941435337764162 1104 62-63 0.4775412067408311 1104 64-65 0.44553064037175716 1104 66-67 0.4027415357399704 1104 68-69 0.38147485222032884 1104 70-71 0.324607462691759 1104 72-73 0.3871113974162981 1104 74-75 0.3951426290482907 1104 76-77 0.2924524480848447 1104 78-79 0.38204748233140506 1104 80-81 0.36397353324325366 1104 82-83 0.38842365168131465 1104 84-85 0.35954218422551776 1104 86-87 0.3789671068471705 1104 88-89 0.32612231359946975 1104 90-91 0.36465117959257043 1104 92-93 0.359449079008062 1104 94-95 0.3429120656849882 1104 96-97 0.3526653352479592 1104 98-99 0.28274245542763055 1104 100-101 0.05466686075733662 1105 1 0.10662691036185379 1105 2 0.07896963988807215 1105 3 0.11066899626224824 1105 4 0.12634036876310972 1105 5 0.14544840037845574 1105 6 0.16448142071904925 1105 7 0.1556132018382712 1105 8 0.15459561056852067 1105 9 0.16700961327460817 1105 10-11 0.23024712398241576 1105 12-13 0.17849852540351918 1105 14-15 0.23581836592851602 1105 16-17 0.25859169979526087 1105 18-19 0.1993837944845609 1105 20-21 0.1974783687409314 1105 22-23 0.2512617184147885 1105 24-25 0.20906805540879958 1105 26-27 0.2571748310217501 1105 28-29 0.20490424028229626 1105 30-31 0.2283876485302372 1105 32-33 0.22526786523911113 1105 34-35 0.206999479731671 1105 36-37 0.21179819003589984 1105 38-39 0.1916003887801807 1105 40-41 0.3078705764406351 1105 42-43 0.21157847553309495 1105 44-45 0.21448084628163144 1105 46-47 0.17750518385393121 1105 48-49 0.2579656150760741 1105 50-51 0.26190132685094625 1105 52-53 0.265198214739506 1105 54-55 0.3358218431326918 1105 56-57 0.2717049596369492 1105 58-59 0.31563331685585894 1105 60-61 0.27227511072106125 1105 62-63 0.2153075369065931 1105 64-65 0.26763009350508327 1105 66-67 0.2419491493907131 1105 68-69 0.2482374195390875 1105 70-71 0.1950026927317623 1105 72-73 0.23656751166522128 1105 74-75 0.2546494876600782 1105 76-77 0.26481909189667974 1105 78-79 0.24885431140048908 1105 80-81 0.2611678065249414 1105 82-83 0.24195311069160397 1105 84-85 0.24905394240571255 1105 86-87 0.24295710413792904 1105 88-89 0.21231985579260382 1105 90-91 0.2047033759493111 1105 92-93 0.17443923755387303 1105 94-95 0.08903130775711787 1105 96-97 0.11400687546097643 1105 98-99 0.0783106973510641 1105 100-101 -0.023342892651765368 1106 1 0.09990429018687763 1106 2 0.050927113653905565 1106 3 0.06582692969434589 1106 4 0.09845117418653615 1106 5 0.12906695720201355 1106 6 0.08600807040784275 1106 7 0.113336212966054 1106 8 0.08651227229287883 1106 9 0.11947608499642826 1106 10-11 0.11188900722012818 1106 12-13 0.1385641043826098 1106 14-15 0.11010270850434978 1106 16-17 0.0903517273559089 1106 18-19 0.08466518004677681 1106 20-21 0.12301948000307306 1106 22-23 0.11779760037604348 1106 24-25 0.08442115325826904 1106 26-27 0.09677322551895173 1106 28-29 0.1395840911464603 1106 30-31 0.12566099324925517 1106 32-33 0.07594814027450525 1106 34-35 0.13803289266530072 1106 36-37 0.09367022142942005 1106 38-39 0.10069809532544838 1106 40-41 0.18335301163445195 1106 42-43 0.1105780765445914 1106 44-45 0.07477494920026118 1106 46-47 0.10496314737864054 1106 48-49 0.11337327247113649 1106 50-51 0.08206854405932518 1106 52-53 0.14309908046175934 1106 54-55 0.13910810160486875 1106 56-57 0.16807271945258861 1106 58-59 0.17065388841624696 1106 60-61 0.19895732473285221 1106 62-63 0.180967626873759 1106 64-65 0.16918760348715267 1106 66-67 0.10784753860335883 1106 68-69 0.12361908347215689 1106 70-71 0.14020254169136592 1106 72-73 0.14234703703315432 1106 74-75 0.109720478646814 1106 76-77 0.12043220084742146 1106 78-79 0.11102632356081088 1106 80-81 0.13188171363509582 1106 82-83 0.02060487434642866 1106 84-85 0.09911394495080117 1106 86-87 0.15370146748784919 1106 88-89 0.06777248193874996 1106 90-91 0.11229012128350391 1106 92-93 0.035468459676376796 1106 94-95 0.06413185052350201 1106 96-97 -0.024544269477523528 1106 98-99 -0.10976007223283091 1106 100-101 -0.0018590976129253534 1107 1 0.008290580593925512 1107 2 -0.0367124255882203 1107 3 -0.0015515601753577357 1107 4 0.04144016208842061 1107 5 0.0138741122947863 1107 6 0.05712348539599077 1107 7 0.05770959404684817 1107 8 0.040143743833915835 1107 9 0.11120037458798748 1107 10-11 0.09273721110233879 1107 12-13 0.07726145813209939 1107 14-15 0.03146106913423097 1107 16-17 -0.0014530269377814875 1107 18-19 -0.020337348667361255 1107 20-21 -0.02658259561960108 1107 22-23 0.016914952598789057 1107 24-25 -0.03671391877124819 1107 26-27 -0.010592729033866988 1107 28-29 -0.0473702118674737 1107 30-31 -0.047731993071060685 1107 32-33 -0.046781103556682524 1107 34-35 -0.04196740228361051 1107 36-37 -0.0568791645265776 1107 38-39 -0.020973387520420772 1107 40-41 0.0064679569104271195 1107 42-43 -0.05019787345198523 1107 44-45 -0.016009072342782815 1107 46-47 -0.0599920211609728 1107 48-49 -0.06303317181881596 1107 50-51 -0.0555754604588401 1107 52-53 -0.016973601189583576 1107 54-55 0.025451031906854382 1107 56-57 0.04483798923237714 1107 58-59 -0.056381154570992464 1107 60-61 -0.03831505272506064 1107 62-63 5.331508233012983E-4 1107 64-65 -0.044558603536749786 1107 66-67 -0.015938057208693124 1107 68-69 -0.016088623858287576 1107 70-71 -0.08496582923912399 1107 72-73 -0.07582196273241237 1107 74-75 -0.20644107450037907 1107 76-77 -0.12497941869699503 1107 78-79 -0.03676114694696864 1107 80-81 -0.07101509603553069 1107 82-83 -0.16372839293291008 1107 84-85 -0.15900117145682202 1107 86-87 -0.1684356892793062 1107 88-89 -0.1660849375013136 1107 90-91 -0.19316536830723763 1107 92-93 -0.2469313985330075 1107 94-95 -0.16853333616862187 1107 96-97 -0.21268450880102918 1107 98-99 -0.2745963169714827 1107 100-101 -0.2752730711352065 1108 1 -0.07651016238813924 1108 2 -0.11352479935692372 1108 3 -0.16351879152162496 1108 4 -0.10469806429156847 1108 5 -0.08581655924092502 1108 6 -0.055108795591706894 1108 7 -0.05641845729716266 1108 8 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42-43 -0.7143552316831077 2316 44-45 -0.6620642487581421 2316 46-47 -0.7453586645786388 2316 48-49 -0.6395306270597771 2316 50-51 -0.7490918297803262 2316 52-53 -0.666371954904001 2316 54-55 -0.6120525691531711 2316 56-57 -0.6163839283107606 2316 58-59 -0.5704632056659378 2316 60-61 -0.8261917612314917 2316 62-63 -0.6687656030332079 2316 64-65 -0.6899477219229695 2316 66-67 -0.5976180222147605 2316 68-69 -0.614517123031753 2316 70-71 -0.5072932629728442 2316 72-73 -0.47715845227341447 2316 74-75 -0.4460477825814735 2316 76-77 -0.5698408444867304 2316 78-79 -0.4837890418854158 2316 80-81 -0.4807685319326751 2316 82-83 -0.32057335171964496 2316 84-85 -0.40944829596393717 2316 86-87 -0.3877054535447009 2316 88-89 -0.3478048489086838 2316 90-91 -0.37015447510388455 2316 92-93 -0.44009825992734264 2316 94-95 -0.4623101480557281 2316 96-97 -0.4117146910416558 2316 98-99 -0.3283830988957135 2316 100-101 -0.41400619140977213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128290.0 3 27323.0 4 23567.0 5 31555.0 6 65139.0 7 142273.0 8 278148.0 9 464965.0 10 609343.0 11 573322.0 12 431885.0 13 314536.0 14 252321.0 15 228345.0 16 214881.0 17 214818.0 18 218922.0 19 231037.0 20 251043.0 21 272742.0 22 301999.0 23 337535.0 24 375646.0 25 418948.0 26 476003.0 27 551437.0 28 643103.0 29 764079.0 30 917640.0 31 1119269.0 32 1395666.0 33 1782091.0 34 2362429.0 35 3343134.0 36 4869881.0 37 8523642.0 38 1.1482987E7 39 2653701.0 40 1411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.230871464674856 19.21435867515631 8.555189356842636 37.9995805033262 2 16.64710829346491 16.767101556081087 53.008509139849814 13.577281010604189 3 17.369958849738005 25.90274404858442 30.70071531381383 26.02658178786375 4 23.11189672356952 26.61266103895074 30.03423900255653 20.241203234923212 5 19.674173683037985 28.71082999005549 38.25270777310961 13.362288553796914 6 13.566998157475862 39.81946520065496 32.35923236126246 14.25430428060672 7 10.644322258787879 21.25334958239684 52.775801792441335 15.326526366373946 8 11.585388544629275 22.33728404930951 48.11840797904965 17.95891942701157 9 12.875882840692693 26.036850447962255 41.596664987562335 19.490601723782714 10-11 15.631077240856122 31.831186493293924 35.22636388969591 17.311372376154043 12-13 15.172531746761797 26.581445291899175 40.640722420238724 17.605300541100306 14-15 15.569485740750578 29.839971010923428 39.490495581610055 15.100047666715946 16-17 19.29899670394424 29.537287578290762 36.70011994710732 14.463595770657681 18-19 19.24450038831783 30.104341215960133 36.71120158716073 13.939956808561307 20-21 19.61197448062892 31.04263888156467 34.7951813480552 14.550205289751212 22-23 18.8487610877503 30.035444010072247 34.95596721888955 16.159827683287904 24-25 17.84113403506635 29.78200453566237 34.70876747585721 17.668093953414065 26-27 16.502041612730533 31.411743181130834 35.47898167874535 16.607233527393284 28-29 15.520465745117148 30.890218053038232 35.95139643886222 17.6379197629824 30-31 16.230850604052275 31.06055810133734 34.42318378345462 18.285407511155768 32-33 16.10387988598771 29.555346735019235 34.52040703765804 19.820366341335014 34-35 16.964474445933494 31.36891587125556 33.50054341876958 18.166066264041365 36-37 18.189000175620933 31.748056656927666 33.403774467203185 16.659168700248227 38-39 19.632545348485426 31.597656885321452 33.13558015385132 15.634217612341802 40-41 19.34901497399858 31.94460057381126 33.192451090854526 15.513933361335633 42-43 18.624576791077757 32.00281135964544 33.15541637537172 16.21719547390508 44-45 18.258948721229597 30.94619538084389 33.64773375019346 17.147122147733057 46-47 17.072002546762317 31.303385016953655 34.298287844723625 17.3263245915604 48-49 15.870378822127362 31.89477651003537 34.20909618134817 18.02574848648909 50-51 15.444907540236821 31.820474714977493 34.74991399738525 17.984703747400438 52-53 15.929990892942062 30.775231170623808 34.18979535867789 19.10498257775624 54-55 16.721421226947484 31.8303324425554 32.77065349266892 18.677592837828193 56-57 18.205662922600897 32.43990166029479 32.45337357695743 16.90106184014688 58-59 17.594614763185625 31.85163102002273 34.12137390069798 16.432380316093663 60-61 19.430004110369364 33.35859047372337 30.804423338968018 16.40698207693924 62-63 18.261450563301942 33.90619811901353 31.845380120422046 15.98697119726248 64-65 19.07738804829993 30.901027015796707 33.49968941587452 16.521895520028842 66-67 17.727658216009353 34.41221278234887 29.966704235398122 17.89342476624366 68-69 16.86574663941182 32.841974463091375 33.0335894699949 17.25868942750191 70-71 16.78790057729601 31.239502565059883 33.66289756398186 18.309699293662245 72-73 16.150565781811483 33.61579437541258 31.917568773313672 18.316071069462268 74-75 16.49082588355404 31.64047894953528 32.94129567503537 18.927399491875306 76-77 17.61653514254479 31.95750318812154 31.919290030790766 18.506671638542908 78-79 17.632704768596334 33.629029730879985 31.706104351615288 17.032161148908393 80-81 18.580286140797668 33.54500370161077 31.09999677899972 16.774713378591844 82-83 18.63783685713758 32.68176057828299 32.20565716200227 16.474745402577167 84-85 19.15416147998664 33.967092146016135 30.425569569470095 16.45317680452713 86-87 17.432375686959432 33.36510503401264 32.50659756230995 16.69592171671798 88-89 18.535331875895302 33.445476972606 30.74013829166778 17.27905285983092 90-91 16.584080413804543 34.15703875307433 31.660622990386084 17.598257842735045 92-93 17.653941582857946 32.30870103814841 31.934840668740282 18.10251671025336 94-95 17.067854775517684 34.59869153007959 30.377298261298936 17.956155433103785 96-97 18.275395211696857 33.24290325741631 30.61237906034662 17.86932247054021 98-99 18.500139909993003 33.49650241942947 30.65686377099597 17.346493899581564 100-101 19.168337430727362 34.84858216119643 28.85707447229696 17.126005935779236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 102412.0 1 94400.0 2 80656.0 3 86063.5 4 108661.0 5 136920.5 6 154880.0 7 163611.0 8 177917.0 9 189291.5 10 200444.5 11 211211.0 12 222545.0 13 230582.5 14 250003.5 15 266430.0 16 262981.0 17 265598.5 18 275568.0 19 290156.5 20 314449.5 21 349716.0 22 396832.0 23 451703.0 24 522383.5 25 625712.5 26 734147.0 27 855273.5 28 961455.0 29 1052428.0 30 1173540.5 31 1329792.5 32 1580976.0 33 2130809.5 34 2559629.5 35 3185861.0 36 3299868.0 37 3034944.0 38 2881745.5 39 2228848.0 40 1831244.0 41 1618329.5 42 1281669.0 43 972700.5 44 842707.5 45 779252.5 46 740744.0 47 686661.5 48 648536.5 49 619153.0 50 556333.5 51 480113.0 52 429506.0 53 386662.5 54 337309.5 55 294675.5 56 262698.5 57 232383.0 58 203766.0 59 177297.0 60 140816.5 61 101540.5 62 76423.5 63 58889.0 64 43760.5 65 30277.0 66 19674.0 67 13240.0 68 8741.5 69 5549.5 70 4098.0 71 3722.5 72 3335.0 73 3290.0 74 2963.0 75 1961.0 76 1494.0 77 1276.0 78 1052.5 79 705.0 80 421.0 81 256.5 82 170.0 83 114.0 84 75.0 85 50.5 86 39.5 87 23.5 88 18.0 89 18.5 90 15.5 91 11.5 92 10.5 93 8.5 94 6.5 95 7.5 96 7.0 97 3.5 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0510011025253887 2 0.0015519592576441815 3 0.005340938807641965 4 0.029984106583994738 5 0.07147258690210664 6 0.014094496473373455 7 0.016530269041229173 8 0.0 9 0.0 10-11 3.1715788643954664E-6 12-13 7.506069979069271E-5 14-15 0.0 16-17 9.091859411267004E-5 18-19 7.400350683589422E-5 20-21 0.0029897416761701266 22-23 0.0015773318885593454 24-25 0.00904640011421067 26-27 0.007185740513765328 28-29 6.343157728790933E-6 30-31 0.0024019423933021667 32-33 0.0018120287245246098 34-35 0.0020276960873035015 36-37 2.1143859095969775E-5 38-39 0.0022919943260031237 40-41 0.1991117211067474 42-43 0.0040701928759741815 44-45 0.0018067427597506175 46-47 0.007693193132068603 48-49 0.007974406458045001 50-51 0.018401500571222498 52-53 0.004368321289227356 54-55 0.010426036920222698 56-57 0.00864043801956805 58-59 0.012358585641594335 60-61 0.012936870187869109 62-63 0.02401096638938328 64-65 0.010848914102142093 66-67 0.03556502819237596 68-69 0.02529651302241824 70-71 0.010428151306132295 72-73 0.006194093522164346 74-75 0.023243444304199574 76-77 0.010701964281425103 78-79 0.03976419860883556 80-81 0.09018701658794949 82-83 0.05501420698180376 84-85 0.08499197040806972 86-87 0.0351178355724962 88-89 0.013946489459701665 90-91 0.02017758473528396 92-93 0.02797861154874201 94-95 0.05826718970371871 96-97 0.05328252492184384 98-99 0.061219929626470895 100-101 0.05236699582298835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.7295056E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.58929258028667 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2340068273414 18.454848767783012 2 9.61560667222347 4.5365071825607215 3 3.5331072368703675 2.500305009841899 4 1.891907801715947 1.785150666784178 5 1.220858115959026 1.4399589648187505 6 0.8072331226065648 1.1425234985798804 7 0.5933367341478939 0.9797475674312507 8 0.47023740664669755 0.887405421406735 9 0.36345359750915496 0.7716251925901086 >10 2.642163614014706 12.399907377918566 >50 0.3342412530920514 5.493499388803956 >100 0.24790139849143958 11.567381222254774 >500 0.024509073688818106 4.005748074793309 >1k 0.017241698569317038 7.963009520015044 >5k 0.002151975035100677 3.460311458315378 >10k+ 0.002043472088067172 22.612070686102413 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT 580136 1.2266313840499523 No Hit GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT 530363 1.1213920541715818 No Hit ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 307622 0.6504316222820414 No Hit GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA 278768 0.5894231312465302 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 273631 0.5785615308289307 No Hit ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA 265301 0.5609486962019878 No Hit ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA 195710 0.4138064663672245 No Hit GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG 186269 0.39384454899471943 No Hit GTCGTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTA 184310 0.38970246699781896 No Hit GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT 180618 0.38189615421958695 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 175769 0.3716434969439512 No Hit GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC 152453 0.32234447507578806 No Hit CTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTA 144361 0.3052348642953293 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144278 0.30505937026483276 No Hit ATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGA 142039 0.3003252602132451 No Hit CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC 137783 0.2913264337820004 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132883 0.2809659428249752 No Hit CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT 131792 0.2786591477976049 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 124241 0.2626934197942381 No Hit CGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTC 116743 0.24683975424407995 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 115591 0.24440398167622426 No Hit GGAGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGAT 114157 0.2413719522818622 No Hit CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG 109525 0.23157811674860898 No Hit GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT 103301 0.2184181788472774 No Hit CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA 101459 0.21452348000179977 No Hit ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC 100287 0.2120454197157521 No Hit AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA 98603 0.20848479384399082 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 92527 0.19563778505727955 No Hit GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC 91138 0.19270090302884937 No Hit CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT 88377 0.1868630835324521 No Hit GTCGTATTATCTCTCTATAGTGAGTCGTATTATCTCTCTATAGTGAGTCG 85877 0.18157711875845967 No Hit ATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATC 83902 0.17740120658700564 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 82086 0.1735614817751775 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 82038 0.17345999125151684 No Hit GGGAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAAT 81081 0.17143652393603254 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 71887 0.15199685988319794 No Hit ATACGACTCACTATAGGAGAGATAATACGACTCACTATAGGAGAGATAAT 71398 0.150962925173405 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 70529 0.14912552381796523 No Hit ATACGACTCACTATAGAGAGATAATACGACTCACTATAGAGAGATAATAC 68144 0.14408271342357645 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 67965 0.1437042383457586 No Hit GTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTATTA 64835 0.13708621044872005 No Hit AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA 64799 0.13701009255597454 No Hit GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 60581 0.1280916127892945 No Hit GAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAAT 59965 0.12678915106898275 No Hit GTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTA 59862 0.12657136932029428 No Hit GAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAA 59440 0.12567909846644434 No Hit CTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATA 58513 0.12371906272824797 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 57508 0.121594104889103 No Hit CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT 55461 0.11726595693215798 No Hit CTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTCACT 55391 0.11711794991848619 No Hit CGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGACTC 52868 0.11178335426857303 No Hit AGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAA 51553 0.10900293679745299 No Hit GATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGA 47734 0.10092809700870213 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09364403755014054 0.0 0.0 1.6915087276775823E-5 0.0 2 0.09393793719157453 0.0 0.0 1.6915087276775823E-5 0.0 3 0.0939569666647609 0.0 0.0 2.5372630915163734E-5 0.0 4 0.09469065857539105 0.0 0.0 2.748701682476071E-5 0.0 5 0.094864038219978 0.0 0.0 3.171578864395466E-5 0.0 6 0.09500358769001141 0.0 0.0 3.171578864395466E-5 0.0 7 0.09585568521157899 0.0 0.0 4.8630875920730486E-5 0.0 8 0.10607239792675158 0.0 0.0 5.497403364952142E-5 0.0 9 0.10619080353768902 0.0 0.0 7.61178927454912E-5 0.0 10-11 0.10777765016284155 0.0 0.0 7.61178927454912E-5 0.0 12-13 0.10919111714340712 0.0 0.0 8.880420820307307E-5 0.0 14-15 0.11036037255141426 0.0 0.0 9.620455888666248E-5 0.0 16-17 0.11103803323544008 0.0 0.0 1.0149052366065494E-4 0.0 18-19 0.11178018268970863 0.0 0.0 1.1523403207303528E-4 0.0 20-21 0.1127697152954 0.0 0.0 1.3954947003340054E-4 0.0 22-23 0.11401085982433343 0.0 0.0 1.4800701367178844E-4 0.0 24-25 0.11510928330436906 1.057192954798489E-6 0.0 1.691508727677582E-4 0.0 26-27 0.11609670152415086 2.114385909596978E-6 0.0 2.6006946688042823E-4 0.0 28-29 0.11715600886485894 2.114385909596978E-6 0.0 3.277298159875316E-4 0.0 30-31 0.11808316708621722 2.114385909596978E-6 0.0 3.446449032643074E-4 0.0 32-33 0.11866779479022077 4.228771819193956E-6 0.0 3.499308680382998E-4 0.0 34-35 0.11904309828917424 4.228771819193956E-6 0.0 3.583884116766877E-4 0.0 36-37 0.11932854038696983 4.228771819193956E-6 0.0 3.6261718349588166E-4 0.0 38-39 0.11963829792272579 4.228771819193956E-6 0.0 3.6578876236027715E-4 0.0 40-41 0.12024301229287053 4.228771819193956E-6 0.0 3.7953227077265747E-4 0.0 42-43 0.12138900945587208 4.228771819193956E-6 0.0 3.964473580494333E-4 0.0 44-45 0.12248108977817893 4.228771819193956E-6 0.0 4.049049016878213E-4 0.0 46-47 0.12368734693960401 4.228771819193956E-6 0.0 4.355634973769774E-4 0.0 48-49 0.12504583988652007 4.228771819193956E-6 0.0 4.524785846537532E-4 0.0 50-51 0.1264233623066225 6.343157728790934E-6 0.0 4.5776454942774564E-4 0.0 52-53 0.1275979036794036 7.4003506835894225E-6 0.0 4.619933212469396E-4 0.0 54-55 0.1289320811883593 8.457543638387911E-6 0.0 4.6727928602093206E-4 0.0 56-57 0.13079274078880465 9.5147365931864E-6 0.0 4.7362244374972304E-4 0.0 58-59 0.13552790803334708 1.2686315457581867E-5 0.0 4.8313718034290946E-4 0.0 60-61 0.1411976338499314 1.2686315457581867E-5 0.0 4.873659521621034E-4 0.0 62-63 0.14393999237467864 1.2686315457581867E-5 0.0 5.21196126715655E-4 0.0 64-65 0.14615164003611708 1.6915087276775823E-5 0.0 5.476259505856173E-4 0.0 66-67 0.15549088259880695 1.90294731863728E-5 0.0 5.6665542377199E-4 0.0 68-69 0.1683918082262129 1.90294731863728E-5 0.0 5.75112967410378E-4 0.0 70-71 0.1713234042898691 2.114385909596978E-5 0.0 5.814561251391688E-4 0.0 72-73 0.17358685440609267 2.5372630915163734E-5 0.0 5.899136687775568E-4 0.0 74-75 0.180358175281577 2.5372630915163734E-5 0.0 6.078859490091311E-4 0.0 76-77 0.18850384699829936 2.5372630915163734E-5 0.0 7.051477008505921E-4 0.0 78-79 0.19509121629964873 2.748701682476071E-5 0.0 7.527213838165242E-4 0.0 80-81 0.20403295431133436 2.748701682476071E-5 0.0 8.055810315564485E-4 0.0 82-83 0.214637656840918 2.85442097795592E-5 0.0 8.446971708839927E-4 0.0 84-85 0.26280125347562755 2.960140273435769E-5 0.0 8.49983135657985E-4 0.0 86-87 0.35928068253053763 3.0658595689156175E-5 0.0 8.531547145223806E-4 0.0 88-89 0.5001431862137979 3.171578864395466E-5 0.0 8.6055506520597E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 65725 0.0 59.195976 1 CCCGAAA 10910 0.0 50.851524 1 CGGTCGT 735 0.0 50.40726 1 GCGTGTT 81860 0.0 47.81738 2 CCGAAAC 14090 0.0 38.902042 2 CGCCGTA 8120 0.0 34.81996 46-47 CGAAACC 15855 0.0 34.54146 3 CGCGCGT 985 0.0 33.273518 1 TCGTCTA 12080 0.0 33.229637 44-45 CGGCCTT 4515 0.0 33.03374 1 CGAGCTA 12275 0.0 32.96498 12-13 CGTGTTT 120350 0.0 32.89949 3 ACTCGTC 12250 0.0 32.63091 42-43 CTCGTAT 5465 0.0 32.245537 1 CTAACGA 11540 0.0 32.23939 94-95 GTCGCCG 9055 0.0 32.16659 44-45 CCAAACG 17425 0.0 31.407625 8 GTTTTTT 630800 0.0 30.870684 1 TCGGTGG 11030 0.0 30.116667 38-39 CGAGTCG 4980 0.0 29.472364 1 >>END_MODULE