FastQCFastQC Report
Fri 27 May 2016
SRR1233047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47295056
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT5697241.2046163979592286No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT5246781.109371770275523No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT2940180.6216675163678842No Hit
GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA2740200.5793840269477639No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA2735640.5784198669729876No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT2683830.5674652335753657No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA2042420.4318464069479059No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1958060.4140094474145459No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG1815130.38378852960867627No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT1771850.3746374673919405No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1737050.36727940442654305No Hit
GTCGTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTA1734210.36667891882821746No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC1559230.3296813941820896No Hit
ATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGA1506010.31842863237121444No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC1385980.2930496582983219No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1327500.2806847294989988No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA1319310.27895304743903887No Hit
CTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTA1315280.2781009499174713No Hit
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT1234000.2609152212442671No Hit
CGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTC1185830.2507302243177384No Hit
GGAGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGAT1179470.24938547487923474No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC1176350.2487257864754405No Hit
CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA1090810.23063932940474793No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG1076520.22761787193993385No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC1062950.22474865026061078No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT1046220.221211282633855No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1034980.21883471287146802No Hit
AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA1034880.21881356901237203No Hit
GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC945620.19994056038330943No Hit
CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT886190.18737476492257457No Hit
ATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATC878760.185803776191744No Hit
GGGAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAAT876450.1853153530466271No Hit
GTCGTATTATCTCTCTATAGTGAGTCGTATTATCTCTCTATAGTGAGTCG830320.17556169084565626No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT815330.1723922263671704No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC774510.16376130308419554No Hit
ATACGACTCACTATAGGAGAGATAATACGACTCACTATAGGAGAGATAAT718200.15185519602725495No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG712730.1506986269347054No Hit
ATACGACTCACTATAGAGAGATAATACGACTCACTATAGAGAGATAATAC698330.14765391122488575No Hit
AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA686680.14519065164020525No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT679240.14361754852346512No Hit
GAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAA661210.13980531072846175No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT642420.13583237960432903No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA639280.1351684624287156No Hit
GTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTATTA623770.13188904988293068No Hit
GAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAAT615500.13014045273569397No Hit
GTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTA610290.12903885767679396No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT605630.12805355384292177No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA593960.1255860654864221No Hit
CTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATA588610.12445486902478771No Hit
CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT571220.12077795192799858No Hit
CGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGACTC546620.11557656259039No Hit
AGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAA544060.11503527979753317No Hit
CTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTCACT511150.10807683576904953No Hit
GATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGA498330.1053661930329462No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA490770.10376771728529087No Hit
GATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAAT478480.10116913700239619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT628400.063.17981
CGGTCGT7850.053.836461
CCCGAAA105500.051.3557851
GCGTGTT796350.050.0465852
CCGAAAC138550.038.865812
TATGCCG182650.038.1830346-47
TGCCGTC190300.036.43736648-49
CGTGTTT1162250.034.690343
CGAAACC155200.034.419943
CGGCCTT45050.033.9404221
CCGTCTT205300.033.91588250-51
TCGTCTA119450.033.27773744-45
CGAGCTA121900.033.1086212-13
ACTCGTC121950.032.51541542-43
TCACGGC97050.032.39250630-31
CTAACGA113250.032.3682994-95
CTCGTAT219450.031.11773342-43
ATCTCGT214750.031.05615840-41
CGTCTGA230550.030.58225416-17
CCAAACG176250.030.5231198
Produced by FastQC (version 0.11.5)