##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233047_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47295056 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09885845150495 33.0 31.0 34.0 30.0 34.0 2 32.36513873670009 34.0 31.0 34.0 31.0 34.0 3 32.18984538257022 34.0 31.0 34.0 30.0 34.0 4 35.860590671464685 37.0 35.0 37.0 35.0 37.0 5 35.78844456807494 37.0 35.0 37.0 35.0 37.0 6 35.859799468257314 37.0 35.0 37.0 35.0 37.0 7 35.96698115760768 37.0 35.0 37.0 35.0 37.0 8 35.961397445009894 37.0 35.0 37.0 35.0 37.0 9 37.673755138380635 39.0 37.0 39.0 35.0 39.0 10-11 37.60010268303731 39.0 37.0 39.0 35.0 39.0 12-13 37.56184758508373 39.0 37.0 39.0 35.0 39.0 14-15 38.85156111243425 40.0 38.0 41.0 35.0 41.0 16-17 38.69549309762949 40.0 38.0 41.0 34.0 41.0 18-19 38.46472932604203 40.0 38.0 41.0 34.0 41.0 20-21 38.24638706422084 40.0 37.5 41.0 34.0 41.0 22-23 38.06506866172227 40.0 37.0 41.0 33.5 41.0 24-25 37.98987170033163 40.0 37.0 41.0 33.5 41.0 26-27 37.651220193078956 40.0 37.0 41.0 33.0 41.0 28-29 37.35612509899555 40.0 37.0 41.0 32.5 41.0 30-31 37.06868936786965 40.0 37.0 41.0 31.5 41.0 32-33 36.81970523515186 40.0 37.0 41.0 31.0 41.0 34-35 36.61241379014331 40.0 37.0 41.0 30.0 41.0 36-37 36.453848875874044 40.0 37.0 41.0 30.0 41.0 38-39 36.30033915172867 40.0 36.0 41.0 30.0 41.0 40-41 36.1603957293126 40.0 36.0 41.0 30.0 41.0 42-43 36.02574793441411 40.0 36.0 41.0 29.0 41.0 44-45 35.892981202940106 40.0 36.0 41.0 29.0 41.0 46-47 35.92442170911056 40.0 36.0 41.0 29.0 41.0 48-49 35.93649770707535 40.0 36.0 41.0 28.5 41.0 50-51 35.84219212046181 40.0 36.0 41.0 28.0 41.0 52-53 35.69609368894711 40.0 35.0 41.0 27.0 41.0 54-55 35.5587234001795 40.0 35.0 41.0 27.0 41.0 56-57 35.393860385745185 39.0 35.0 41.0 26.0 41.0 58-59 35.160475462805245 39.0 35.0 41.0 26.0 41.0 60-61 34.94129935061288 39.0 35.0 41.0 25.0 41.0 62-63 34.654892120225 39.0 35.0 40.0 23.5 41.0 64-65 34.36572929525657 38.0 34.0 40.0 22.5 41.0 66-67 34.095338495846164 38.0 34.0 40.0 22.0 41.0 68-69 33.70461064682955 37.5 34.0 40.0 21.0 41.0 70-71 33.31978870053563 37.0 33.5 39.0 19.5 41.0 72-73 32.93392344223041 36.5 33.0 39.0 18.0 41.0 74-75 32.51996680160396 36.0 33.0 39.0 15.5 40.0 76-77 31.766373667048832 35.0 31.5 38.0 13.0 39.5 78-79 31.738923831700294 35.0 32.0 37.0 9.0 39.0 80-81 31.446297515748793 35.0 32.0 37.0 7.0 39.0 82-83 31.005593185046656 35.0 32.0 36.0 4.0 39.0 84-85 30.60643441251026 35.0 31.0 36.0 2.0 37.5 86-87 30.134430129441014 35.0 31.0 36.0 2.0 37.0 88-89 29.88881187708077 35.0 31.0 35.0 2.0 37.0 90-91 29.545418024243382 34.0 31.0 35.0 2.0 36.0 92-93 29.192353646858987 34.0 30.5 35.0 2.0 36.0 94-95 28.842290682560986 34.0 30.0 35.0 2.0 36.0 96-97 28.422025306408347 34.0 29.5 35.0 2.0 36.0 98-99 27.50085671745478 34.0 29.0 35.0 2.0 35.0 100-101 25.29525851497036 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3887142404905646 1101 2 0.27225367783831445 1101 3 0.555623366207918 1101 4 0.3267840559286128 1101 5 0.2362964161423875 1101 6 0.22768294968982872 1101 7 0.1814209062317218 1101 8 0.20801703067204613 1101 9 0.2320411868244534 1101 10-11 0.208122277567945 1101 12-13 0.210124602825708 1101 14-15 0.300204996705439 1101 16-17 0.3643653088257679 1101 18-19 0.37683623867055616 1101 20-21 0.37648837182633343 1101 22-23 0.4310643705177952 1101 24-25 0.3753080382582539 1101 26-27 0.3967103122379285 1101 28-29 0.4065865269795097 1101 30-31 0.4062997218957989 1101 32-33 0.42608187593264546 1101 34-35 0.44588745673001995 1101 36-37 0.4421013714917166 1101 38-39 0.4290046428576275 1101 40-41 0.3670600579672083 1101 42-43 0.3499636798991972 1101 44-45 0.3894004369124815 1101 46-47 0.4540238752725756 1101 48-49 0.4741705472973763 1101 50-51 0.5038105001464572 1101 52-53 0.49094965336602314 1101 54-55 0.47885261272733004 1101 56-57 0.4696235513940721 1101 58-59 0.4824561105244527 1101 60-61 0.5268189929243903 1101 62-63 0.49778531967773887 1101 64-65 0.4574150691183263 1101 66-67 0.4770919509644216 1101 68-69 0.46857193767704075 1101 70-71 0.45684482258678827 1101 72-73 0.4814833788101538 1101 74-75 0.47588465568730953 1101 76-77 0.5118538827453172 1101 78-79 0.47388495811156517 1101 80-81 0.4669199804518094 1101 82-83 0.4007862979950012 1101 84-85 0.36723220177426086 1101 86-87 0.39158347567888185 1101 88-89 0.443989901026427 1101 90-91 0.4060037781856387 1101 92-93 0.44636647658200346 1101 94-95 0.49776759168099005 1101 96-97 0.506506910228083 1101 98-99 0.4582745028109052 1101 100-101 0.8009293349918956 1102 1 0.33150512827835144 1102 2 0.2391972332215886 1102 3 0.5123247921689327 1102 4 0.32245269859193826 1102 5 0.1744716466334637 1102 6 0.2186620235777923 1102 7 0.1841733190556667 1102 8 0.18464403874624224 1102 9 0.24674250547516152 1102 10-11 0.23328911796074436 1102 12-13 0.23621791697180328 1102 14-15 0.2702339343153426 1102 16-17 0.30358563315608933 1102 18-19 0.33472183985843884 1102 20-21 0.33042031177355824 1102 22-23 0.3484529139974555 1102 24-25 0.36541366656309293 1102 26-27 0.3697691683221507 1102 28-29 0.36216847135781194 1102 30-31 0.3681733115641066 1102 32-33 0.3771420052717005 1102 34-35 0.4064412321536679 1102 36-37 0.4169930091856102 1102 38-39 0.40684083307659336 1102 40-41 0.379933339570556 1102 42-43 0.32186113103834657 1102 44-45 0.3437013675580758 1102 46-47 0.3938940539201212 1102 48-49 0.4020089982671138 1102 50-51 0.43043639291693836 1102 52-53 0.4254727705977075 1102 54-55 0.3743308157741012 1102 56-57 0.40252651816224017 1102 58-59 0.427773559119494 1102 60-61 0.4539631522596892 1102 62-63 0.4770363540465681 1102 64-65 0.435594483505497 1102 66-67 0.46008642689526624 1102 68-69 0.40383243946725145 1102 70-71 0.38066133161381543 1102 72-73 0.43353228216516726 1102 74-75 0.44145109432963636 1102 76-77 0.4288271293220909 1102 78-79 0.43454708076965787 1102 80-81 0.47400238813330375 1102 82-83 0.4274289263498794 1102 84-85 0.348562325042451 1102 86-87 0.3781483643504693 1102 88-89 0.41103848787633623 1102 90-91 0.3951464592913716 1102 92-93 0.4540938011010205 1102 94-95 0.4983392963654474 1102 96-97 0.5103632205260737 1102 98-99 0.3003070684736535 1102 100-101 0.6162349854822402 1103 1 0.3147465368862825 1103 2 0.25370693916820386 1103 3 0.5234044468523535 1103 4 0.3279667785423044 1103 5 0.2080784967285112 1103 6 0.20549503713023398 1103 7 0.16345617472945406 1103 8 0.15465252802832197 1103 9 0.22069250830570297 1103 10-11 0.22396941028773654 1103 12-13 0.2000939760267002 1103 14-15 0.24297860646624514 1103 16-17 0.29303020743110864 1103 18-19 0.3122512073004913 1103 20-21 0.33457307721158713 1103 22-23 0.3055830816637055 1103 24-25 0.31614545835135033 1103 26-27 0.3132871371076078 1103 28-29 0.3343146900598697 1103 30-31 0.2988888897359132 1103 32-33 0.28439481370586606 1103 34-35 0.32959475248046033 1103 36-37 0.3623081185169923 1103 38-39 0.36623146567197296 1103 40-41 0.31233043655890214 1103 42-43 0.31101425438768615 1103 44-45 0.35395908358795936 1103 46-47 0.3979960193444825 1103 48-49 0.3655190298428508 1103 50-51 0.40410632897479815 1103 52-53 0.4104453974000748 1103 54-55 0.39432345285998593 1103 56-57 0.3841770215071918 1103 58-59 0.37011573075692183 1103 60-61 0.42335730665561044 1103 62-63 0.4164023410560347 1103 64-65 0.41308361282656847 1103 66-67 0.37044368905618086 1103 68-69 0.3734209689476842 1103 70-71 0.3428536834467195 1103 72-73 0.38314448174774185 1103 74-75 0.3979959881048316 1103 76-77 0.440971723387495 1103 78-79 0.37373906556801373 1103 80-81 0.4402035724220674 1103 82-83 0.31814822048326974 1103 84-85 0.2948815381896317 1103 86-87 0.34481537372306903 1103 88-89 0.4159900353398207 1103 90-91 0.379954687836225 1103 92-93 0.38793268065614583 1103 94-95 0.4256423164819303 1103 96-97 0.38928808885915345 1103 98-99 0.2593982661478407 1103 100-101 0.5192645491906873 1104 1 0.30946187747994003 1104 2 0.2610180542585496 1104 3 0.49523423547325507 1104 4 0.32243791579165304 1104 5 0.21340790769317408 1104 6 0.19574554117917842 1104 7 0.1385378084612583 1104 8 0.1615520186118573 1104 9 0.196154073710467 1104 10-11 0.2127533115605118 1104 12-13 0.20230162615266067 1104 14-15 0.24559262123188574 1104 16-17 0.27156810653698216 1104 18-19 0.2707862307401214 1104 20-21 0.31427398602158974 1104 22-23 0.27056173558612073 1104 24-25 0.2498122345954883 1104 26-27 0.24718128475003454 1104 28-29 0.2334578866574475 1104 30-31 0.227083815437787 1104 32-33 0.24127031936952648 1104 34-35 0.26549991399513573 1104 36-37 0.2963052641574748 1104 38-39 0.27029183625170106 1104 40-41 0.26923346094167044 1104 42-43 0.23974602061468886 1104 44-45 0.23226283137507409 1104 46-47 0.21856253598799924 1104 48-49 0.24178586717123096 1104 50-51 0.25233230051345856 1104 52-53 0.2615913784235673 1104 54-55 0.24658089726091958 1104 56-57 0.2307441712649947 1104 58-59 0.1730712322809822 1104 60-61 0.28317264745714255 1104 62-63 0.2587865879729705 1104 64-65 0.22314931142527428 1104 66-67 0.2725275541297876 1104 68-69 0.27830026965929733 1104 70-71 0.2219433192293181 1104 72-73 0.22490079258606954 1104 74-75 0.2376315090083665 1104 76-77 0.2402872984888198 1104 78-79 0.2631875530945216 1104 80-81 0.3610966982322985 1104 82-83 0.2536624870098301 1104 84-85 0.23556581795012477 1104 86-87 0.24271703009494416 1104 88-89 0.2914664727683345 1104 90-91 0.3160311142624508 1104 92-93 0.38758737242050856 1104 94-95 0.4066803599835964 1104 96-97 0.42908448114709685 1104 98-99 0.3510388320694524 1104 100-101 0.49757139276409745 1105 1 0.20689961511072852 1105 2 0.17400436191582003 1105 3 0.4406953793287727 1105 4 0.2699438843593924 1105 5 0.16175647584094577 1105 6 0.12165170965742078 1105 7 0.074145492461831 1105 8 0.06352243030816851 1105 9 0.10685028869520607 1105 10-11 0.09378992119209073 1105 12-13 0.12385313596650604 1105 14-15 0.08340240368335117 1105 16-17 0.15009345862345214 1105 18-19 0.19901180357968684 1105 20-21 0.1864955931223662 1105 22-23 0.17395593952504385 1105 24-25 0.1515093726906187 1105 26-27 0.14690793952113523 1105 28-29 0.12465075480261589 1105 30-31 0.1271075631486056 1105 32-33 0.15280478415326115 1105 34-35 0.19255293598440915 1105 36-37 0.18357247251842068 1105 38-39 0.16021781875627283 1105 40-41 0.16497206452250168 1105 42-43 0.1688077728198465 1105 44-45 0.16007231525293975 1105 46-47 0.2047616313625582 1105 48-49 0.177769320581028 1105 50-51 0.20077028082683768 1105 52-53 0.22022084669058728 1105 54-55 0.2573466097640207 1105 56-57 0.270290077431234 1105 58-59 0.27262667343450886 1105 60-61 0.28620254010741064 1105 62-63 0.276790950379727 1105 64-65 0.25972387214754633 1105 66-67 0.29054662917531715 1105 68-69 0.2940414029106364 1105 70-71 0.2812449325880024 1105 72-73 0.2428310042555637 1105 74-75 0.2594799713573437 1105 76-77 0.2780210921143045 1105 78-79 0.2631113644414178 1105 80-81 0.3035017638893791 1105 82-83 0.23221923449279558 1105 84-85 0.20087255957280092 1105 86-87 0.25059442518638875 1105 88-89 0.24976907804279946 1105 90-91 0.2562125313215269 1105 92-93 0.3336676538179937 1105 94-95 0.31105651551974134 1105 96-97 0.3144542018795562 1105 98-99 0.17267986416318948 1105 100-101 0.2254809765736958 1106 1 0.14691256302064204 1106 2 0.11174802129889372 1106 3 0.374822107293177 1106 4 0.24773018432468064 1106 5 0.1354122479341271 1106 6 0.07821198190787726 1106 7 0.05005086100812406 1106 8 0.06778187218542087 1106 9 0.09659621269647545 1106 10-11 0.04650394443321204 1106 12-13 0.05125386478509597 1106 14-15 0.0735780427092223 1106 16-17 0.04009103557469729 1106 18-19 0.05997829219029427 1106 20-21 0.0811814537206601 1106 22-23 0.08926331390359366 1106 24-25 0.06558573109187193 1106 26-27 0.11874362259683124 1106 28-29 0.1208897474320807 1106 30-31 0.09668991881986244 1106 32-33 0.10183667504289673 1106 34-35 0.11028240676192524 1106 36-37 0.12279009058816825 1106 38-39 0.1484875857622896 1106 40-41 0.14959687894727836 1106 42-43 0.11799159539711468 1106 44-45 0.11746907834376685 1106 46-47 0.11559819685962225 1106 48-49 0.13196685327547897 1106 50-51 0.11082904917180514 1106 52-53 0.11073353557844001 1106 54-55 0.1251513562775841 1106 56-57 0.1375179501047441 1106 58-59 0.16772876853948304 1106 60-61 0.18024282056710206 1106 62-63 0.15353482275160246 1106 64-65 0.13415778989954674 1106 66-67 0.151444821224473 1106 68-69 0.17918750680163953 1106 70-71 0.19382210459278326 1106 72-73 0.19243871555494962 1106 74-75 0.20564875815093586 1106 76-77 0.15184631652963887 1106 78-79 0.09237104962884146 1106 80-81 0.18178444975959707 1106 82-83 0.0896638090479982 1106 84-85 0.18231960457567808 1106 86-87 0.20445061841882506 1106 88-89 0.24485949137153185 1106 90-91 0.2129054872421321 1106 92-93 0.26535111277346957 1106 94-95 0.2253625242399444 1106 96-97 0.26181684789915494 1106 98-99 0.13408037769694658 1106 100-101 0.2986746618167082 1107 1 0.11869417061577536 1107 2 0.07706929754648684 1107 3 0.3217111309774481 1107 4 0.2040640885803171 1107 5 0.07132661276209973 1107 6 0.05138551441462624 1107 7 0.05353208752507754 1107 8 0.026112074895358717 1107 9 0.02923710348184727 1107 10-11 0.013160909003794075 1107 12-13 -0.01075672710159381 1107 14-15 -0.03534462767711233 1107 16-17 -0.021799204384372217 1107 18-19 0.012564658424082609 1107 20-21 0.057438750500367064 1107 22-23 0.04705968246376102 1107 24-25 0.04141286907519515 1107 26-27 0.05981165553867385 1107 28-29 0.03168835774289391 1107 30-31 -0.030976668468994717 1107 32-33 0.01361371234285258 1107 34-35 -0.013141720428940573 1107 36-37 -0.013087173904381189 1107 38-39 0.031576163621210185 1107 40-41 0.02010814253235793 1107 42-43 0.0544665861921203 1107 44-45 0.06150175993170848 1107 46-47 0.011097560365399772 1107 48-49 0.0018818791109325161 1107 50-51 -0.003095560079138693 1107 52-53 0.054000637813011565 1107 54-55 0.06808282298134571 1107 56-57 0.06821536259119654 1107 58-59 0.05631649193924915 1107 60-61 0.1027503618136052 1107 62-63 0.13856269766856855 1107 64-65 0.024427186894747877 1107 66-67 0.05117325063055489 1107 68-69 0.061389367758934554 1107 70-71 0.05154547919374863 1107 72-73 0.1394610385088484 1107 74-75 0.18185298195959376 1107 76-77 0.09653453965727365 1107 78-79 0.13485726355581207 1107 80-81 0.20696986062644385 1107 82-83 0.14163691764391828 1107 84-85 0.17493239949809336 1107 86-87 0.13380309931173784 1107 88-89 0.17753964970222924 1107 90-91 0.1028844829831641 1107 92-93 0.227552358986145 1107 94-95 0.1847762964686268 1107 96-97 0.2011281061000716 1107 98-99 0.12362125022375992 1107 100-101 0.19269073955518579 1108 1 0.06820169526329778 1108 2 0.02022573908363512 1108 3 0.267989671211609 1108 4 0.16061409800625626 1108 5 0.05341385877692062 1108 6 0.012895619801476244 1108 7 0.014637505327286249 1108 8 0.01392484038686348 1108 9 0.0029648733037745956 1108 10-11 -0.050127302537781304 1108 12-13 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-0.15667091501941854 2316 98-99 0.09941758168126924 2316 100-101 -0.041209778574543776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3948.0 3 719.0 4 1539.0 5 4400.0 6 13065.0 7 42287.0 8 135304.0 9 304048.0 10 510594.0 11 530448.0 12 394645.0 13 267882.0 14 206410.0 15 185244.0 16 181562.0 17 179140.0 18 177433.0 19 182187.0 20 194091.0 21 208296.0 22 231282.0 23 262342.0 24 298749.0 25 347078.0 26 410144.0 27 490234.0 28 590478.0 29 725380.0 30 895439.0 31 1109044.0 32 1379740.0 33 1747914.0 34 2303749.0 35 3262808.0 36 4813457.0 37 8540033.0 38 1.2918184E7 39 3242128.0 40 3631.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.10472996032295 20.00237752751855 9.203103692593727 37.68978881956478 2 17.163900629101942 17.59135654369331 51.499736772918446 13.74500605428631 3 17.62331938028532 26.641199353314505 30.235507030720356 25.499974235679822 4 23.394779361293068 27.421747846117363 29.03495663479075 20.148516157798817 5 19.988347733669297 29.28967819970667 37.32866783141402 13.393306235210007 6 13.707897924890924 40.269403634916934 31.641812624135596 14.380885816056546 7 10.681087574648958 21.63938184292245 52.19357068228347 15.485959900145119 8 11.630529899143102 22.755706443911812 47.37152631417024 18.24223734277485 9 13.042645833408217 25.97201304411534 41.28729340527063 19.698047717205814 10-11 15.768184099411997 32.37971639149767 34.40965161347943 17.442447895610908 12-13 15.204994154145837 26.965834441553465 40.07215468779654 17.75701671650415 14-15 15.678112978745396 30.30611209148634 38.70790791040967 15.30786701935859 16-17 19.36562584043624 29.840753907074735 36.04515203946531 14.748468213023717 18-19 19.20474732073475 30.285998604167 36.289671588505996 14.219582486592255 20-21 19.617909593043546 31.113306010225898 34.565345592159815 14.70343880457074 22-23 18.604158535308613 30.425844958820896 34.818850935990824 16.151145569879667 24-25 17.740224558273603 29.690525476661588 34.76925446028147 17.799995504783336 26-27 16.47143836768055 31.153456082174845 35.4186672281348 16.95643832200981 28-29 15.564674599753744 30.796488709984054 35.78351277198954 17.855323918272656 30-31 16.14424526934816 30.9712890303733 34.335560314499354 18.548905385779182 32-33 16.12166713577842 29.748126315782354 34.01068707900462 20.119519469434607 34-35 17.086608402785807 31.354247557350234 33.07132037678444 18.48782366307952 36-37 18.375120887603718 31.86127037669691 32.926622881914795 16.83698585378458 38-39 19.73381860045734 31.883756804510575 32.5967762247527 15.785648370279384 40-41 19.247580407078885 32.323076146443945 32.82310376570829 15.606239680768876 42-43 18.616187376762745 32.12821505781493 32.82840616905431 16.42719139636801 44-45 18.33413902212102 31.051978562006905 33.302744542564625 17.31113787330745 46-47 17.119756617287834 31.53485841671851 34.117377409900726 17.228007556092937 48-49 15.996882098332343 31.97702686472883 34.18516096909596 17.840930067842866 50-51 15.440980010187502 31.853586856624162 34.45218782818015 18.25324530500819 52-53 16.179657071920808 30.622077327122234 33.96005224845863 19.238213352498338 54-55 16.79333019839585 31.73201594037442 32.671564318996175 18.803089542233554 56-57 18.27782555211087 32.29461285337581 32.36221582645541 17.065345768057906 58-59 17.781421118894023 31.603370513881647 34.02991058848956 16.585297778734766 60-61 19.545796155396815 32.96664186630314 30.819572632630145 16.667989345669902 62-63 18.509867741888083 33.52435574947256 31.918344819753536 16.047431688885823 64-65 19.205881617280824 30.966701657659275 33.220427230659915 16.606989494399986 66-67 17.775956963629117 34.541092075594925 29.800906535206543 17.88204442556941 68-69 16.886235921025104 33.038050488284625 32.83786057528707 17.2378530154032 70-71 16.801449818074182 31.47455969757848 33.423428024542815 18.300562459804524 72-73 16.206665346829514 33.725079613253875 31.71136874727149 18.35688629264512 74-75 16.555777908015962 31.63902958162652 32.85772777626956 18.947464734087948 76-77 17.6130069160579 32.11118358208916 31.717758001161712 18.558051500691235 78-79 17.61491256993299 33.730392964282935 31.60971230441092 17.044982161373156 80-81 18.49512705032083 33.66363045074976 31.024288898957497 16.816953599971914 82-83 18.601155918515676 32.83874175162274 32.05005516171517 16.510047168146404 84-85 19.124837526615224 34.029345018002054 30.326028286467988 16.519789168914734 86-87 17.39933033577906 33.50197554286352 32.3471612945601 16.751532826797327 88-89 18.428576206000542 33.64448494043554 30.615867963314198 17.31107089024972 90-91 16.477365508012284 34.35348640519248 31.516602136157505 17.652545950637734 92-93 17.510831566029225 32.467849134299406 31.780681288861413 18.240638010809953 94-95 16.83775294093802 34.727096001003915 30.324520211935457 18.110630846122604 96-97 18.037639495206037 33.24489843452879 30.657156574916804 18.06030549534837 98-99 18.087232175469815 33.67686424626123 30.654178011020367 17.581725567248586 100-101 18.438592818706674 34.86482297273177 29.2145464127174 17.48203779584415 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 144041.0 1 116556.5 2 80799.5 3 82949.5 4 105190.5 5 131154.5 6 148054.0 7 157639.0 8 172117.5 9 183245.5 10 192306.0 11 201579.5 12 210755.0 13 216860.5 14 230993.0 15 243950.0 16 242537.5 17 246473.5 18 257938.5 19 272358.5 20 295957.5 21 331352.5 22 378494.5 23 434586.5 24 508723.5 25 617176.0 26 730478.0 27 854800.5 28 961573.5 29 1051961.0 30 1174644.5 31 1333183.5 32 1585880.0 33 2141995.5 34 2551811.0 35 3199796.5 36 3337058.0 37 3056217.0 38 2894480.0 39 2228713.5 40 1838809.5 41 1630248.0 42 1292247.0 43 981856.0 44 851448.0 45 792383.5 46 756312.0 47 699517.0 48 660516.0 49 628982.0 50 562766.5 51 485179.5 52 434968.0 53 393110.5 54 343394.5 55 299783.0 56 267176.0 57 236375.5 58 207306.0 59 179095.0 60 141220.5 61 101544.0 62 76218.0 63 58846.0 64 43331.5 65 29398.0 66 18659.5 67 12602.0 68 8323.5 69 5039.5 70 3716.5 71 3629.0 72 3417.0 73 3493.0 74 3063.0 75 1792.0 76 1323.0 77 1201.5 78 942.5 79 563.5 80 343.5 81 216.0 82 137.5 83 77.5 84 46.0 85 28.5 86 20.0 87 12.0 88 5.0 89 3.0 90 4.0 91 2.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004710851806582066 2 0.0027275578233801013 3 0.002048839946399471 4 0.0 5 0.002647211158815416 6 0.0 7 4.651649001113351E-5 8 0.0038291528822801267 9 3.509880609930983E-4 10-11 0.0 12-13 0.0 14-15 0.002303623448505907 16-17 1.046621025250504E-4 18-19 0.0 20-21 9.5147365931864E-5 22-23 3.0658595689156175E-5 24-25 7.167768233533755E-4 26-27 0.0 28-29 3.4887367508350134E-5 30-31 3.0658595689156175E-5 32-33 0.0 34-35 6.343157728790933E-6 36-37 0.013310059300912974 38-39 0.0014631550494411086 40-41 3.5627402576709073E-4 42-43 0.0012918897907637534 44-45 0.006813608593676261 46-47 0.005551320205646865 48-49 0.011754928464404398 50-51 0.020446111745802777 52-53 0.04669092684867526 54-55 0.04521825706264097 56-57 0.05118082532770444 58-59 0.05598365292135398 60-61 0.04983924746806516 62-63 0.06474355374481426 64-65 0.03933497826918737 66-67 0.02316838360440888 68-69 0.04059092349948798 70-71 0.04029068070032521 72-73 0.03487890996471175 74-75 0.031748561625553416 76-77 0.03297067668130047 78-79 0.05320217825727915 80-81 0.029365648705437626 82-83 0.02017758473528396 84-85 0.02033827806441333 86-87 0.013282572284088214 88-89 0.008942795204640417 90-91 0.0065218233381518775 92-93 0.009666972378677382 94-95 0.008773644331872658 96-97 0.008371911009049233 98-99 0.21755656658911665 100-101 0.06514845864650208 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.7295056E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.404509576439786 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85009880307099 16.23534166204237 2 10.48444858443574 4.488289602584907 3 3.9346666054644786 2.5265882711028675 4 2.0711183585081865 1.7732509095451492 5 1.3412728916229253 1.4354644226680993 6 0.9674241167814018 1.242434326327579 7 0.6928021830238597 1.0380363672778814 8 0.487783920043724 0.8352620470247383 9 0.4180233633079621 0.8052826574790758 >10 3.044445730899161 12.941383175765184 >50 0.37477864051488174 5.61670330152699 >100 0.2809010555370546 11.826697155918431 >500 0.027999193426337197 4.157374693770188 >1k 0.01948815259445704 8.208355824157751 >5k 0.002374200386198346 3.4439847165628077 >10k+ 0.0023742003833192023 23.425550866245874 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT 569724 1.2046163979592286 No Hit GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT 524678 1.109371770275523 No Hit ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 294018 0.6216675163678842 No Hit GTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTA 274020 0.5793840269477639 No Hit ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA 273564 0.5784198669729876 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 268383 0.5674652335753657 No Hit ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA 204242 0.4318464069479059 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 195806 0.4140094474145459 No Hit GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG 181513 0.38378852960867627 No Hit GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT 177185 0.3746374673919405 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 173705 0.36727940442654305 No Hit GTCGTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTA 173421 0.36667891882821746 No Hit GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC 155923 0.3296813941820896 No Hit ATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGA 150601 0.31842863237121444 No Hit CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC 138598 0.2930496582983219 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132750 0.2806847294989988 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 131931 0.27895304743903887 No Hit CTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATCTCCTA 131528 0.2781009499174713 No Hit CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT 123400 0.2609152212442671 No Hit CGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTC 118583 0.2507302243177384 No Hit GGAGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGAT 117947 0.24938547487923474 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 117635 0.2487257864754405 No Hit CGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTA 109081 0.23063932940474793 No Hit CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG 107652 0.22761787193993385 No Hit ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC 106295 0.22474865026061078 No Hit GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT 104622 0.221211282633855 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 103498 0.21883471287146802 No Hit AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA 103488 0.21881356901237203 No Hit GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC 94562 0.19994056038330943 No Hit CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT 88619 0.18737476492257457 No Hit ATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTATC 87876 0.185803776191744 No Hit GGGAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAAT 87645 0.1853153530466271 No Hit GTCGTATTATCTCTCTATAGTGAGTCGTATTATCTCTCTATAGTGAGTCG 83032 0.17556169084565626 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 81533 0.1723922263671704 No Hit GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 77451 0.16376130308419554 No Hit ATACGACTCACTATAGGAGAGATAATACGACTCACTATAGGAGAGATAAT 71820 0.15185519602725495 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 71273 0.1506986269347054 No Hit ATACGACTCACTATAGAGAGATAATACGACTCACTATAGAGAGATAATAC 69833 0.14765391122488575 No Hit AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA 68668 0.14519065164020525 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 67924 0.14361754852346512 No Hit GAATAATACGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAA 66121 0.13980531072846175 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 64242 0.13583237960432903 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 63928 0.1351684624287156 No Hit GTATTATTCCCTATAGTGAGTCGTATTATTCCCTATAGTGAGTCGTATTA 62377 0.13188904988293068 No Hit GAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAAT 61550 0.13014045273569397 No Hit GTATTATCTCCTATAGTGAGTCGTATTATCTCCTATAGTGAGTCGTATTA 61029 0.12903885767679396 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 60563 0.12805355384292177 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 59396 0.1255860654864221 No Hit CTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATA 58861 0.12445486902478771 No Hit CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT 57122 0.12077795192799858 No Hit CGACTCACTATAGGGAATAATACGACTCACTATAGGGAATAATACGACTC 54662 0.11557656259039 No Hit AGATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAA 54406 0.11503527979753317 No Hit CTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGACTCACT 51115 0.10807683576904953 No Hit GATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGA 49833 0.1053661930329462 No Hit AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA 49077 0.10376771728529087 No Hit GATAATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAAT 47848 0.10116913700239619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09569499188244962 0.0 0.0 8.457543638387911E-6 0.0 2 0.09600369222525078 2.114385909596978E-6 0.0 2.114385909596978E-5 0.0 3 0.09602272169843715 2.114385909596978E-6 0.0 3.594456046314862E-5 0.0 4 0.09692767886774466 2.114385909596978E-6 0.0 4.4402104101536534E-5 0.0 5 0.09711374482778919 2.114385909596978E-6 0.0 5.074526183032747E-5 0.0 6 0.09726386622737057 2.114385909596978E-6 0.0 5.920280546871538E-5 0.0 7 0.09811807813484776 2.114385909596978E-6 0.0 7.188912092629724E-5 0.0 8 0.10950827502984667 2.114385909596978E-6 0.0 7.400350683589422E-5 0.0 9 0.10965205327169926 2.114385909596978E-6 0.0 8.880420820307307E-5 0.0 10-11 0.11140699357666475 2.114385909596978E-6 0.0 9.5147365931864E-5 0.0 12-13 0.11317884896890702 2.114385909596978E-6 0.0 1.0571929547984889E-4 0.0 14-15 0.11454051349468747 2.114385909596978E-6 0.0 1.131196461634383E-4 0.0 16-17 0.11531860750941916 2.114385909596978E-6 0.0 1.1946280389222925E-4 0.0 18-19 0.11610304468187965 2.114385909596978E-6 0.0 1.2897754048541566E-4 0.0 20-21 0.11714860851417536 2.114385909596978E-6 0.0 1.437782418525945E-4 0.0 22-23 0.11842464041061712 2.114385909596978E-6 0.0 1.606933291293703E-4 0.0 24-25 0.1195600656440707 2.114385909596978E-6 0.0 1.712652586773552E-4 0.0 26-27 0.12057708526658685 2.114385909596978E-6 0.0 2.389256077844585E-4 0.0 28-29 0.12166916558889368 2.114385909596978E-6 0.0 2.87556483705189E-4 0.0 30-31 0.12260795293275475 2.114385909596978E-6 0.0 2.960140273435769E-4 0.0 32-33 0.12320632414517069 2.114385909596978E-6 0.0 3.023571850723678E-4 0.0 34-35 0.12353616834706782 2.114385909596978E-6 0.0 3.0658595689156176E-4 0.0 36-37 0.12377720834076188 2.114385909596978E-6 0.0 3.2032946530394213E-4 0.0 38-39 0.12400767640490795 2.114385909596978E-6 0.0 3.2350104416833757E-4 0.0 40-41 0.1245341584963976 4.228771819193956E-6 0.0 3.3513016667112097E-4 0.0 42-43 0.12562835320461402 4.228771819193956E-6 0.0 3.435877103095089E-4 0.0 44-45 0.12666017352849734 4.228771819193956E-6 0.0 3.5415963985749375E-4 2.114385909596978E-6 46-47 0.1278156854280921 4.228771819193956E-6 0.0 3.647315694054787E-4 2.114385909596978E-6 48-49 0.1290917173245339 4.228771819193956E-6 0.0 3.700175341794711E-4 2.114385909596978E-6 50-51 0.13043118079826355 4.228771819193956E-6 0.0 3.7107472713426956E-4 2.114385909596978E-6 52-53 0.13156449164580752 5.285964773992445E-6 0.0 3.7636069190826203E-4 2.114385909596978E-6 54-55 0.13283523757747534 6.343157728790934E-6 0.0 3.816466566822545E-4 2.114385909596978E-6 56-57 0.1346736961258699 6.343157728790934E-6 0.0 3.8587542850144845E-4 2.114385909596978E-6 58-59 0.1395748426643157 6.343157728790934E-6 0.0 3.9116139327544086E-4 2.114385909596978E-6 60-61 0.1453735460213854 6.343157728790934E-6 0.0 4.006761298686273E-4 2.114385909596978E-6 62-63 0.1481180189320423 6.343157728790934E-6 0.0 4.218199889645971E-4 2.114385909596978E-6 64-65 0.15039309817076862 6.343157728790934E-6 0.0 4.376778832865744E-4 2.114385909596978E-6 66-67 0.1598052870473396 6.343157728790934E-6 0.0 4.567073564729472E-4 2.114385909596978E-6 68-69 0.17279607407590342 6.343157728790934E-6 0.0 4.6093612829214113E-4 2.114385909596978E-6 70-71 0.17558600628361662 6.343157728790934E-6 0.0 4.651649001113351E-4 2.114385909596978E-6 72-73 0.1777743957000495 8.457543638387911E-6 0.0 4.7150805784012607E-4 2.114385909596978E-6 74-75 0.18466306499351645 9.5147365931864E-6 0.0 4.7996560147851397E-4 2.114385909596978E-6 76-77 0.19309840757985358 1.057192954798489E-5 0.0 5.444543717212217E-4 2.114385909596978E-6 78-79 0.19984964178919673 1.057192954798489E-5 0.0 5.867420899131613E-4 2.114385909596978E-6 80-81 0.20902079067207363 1.057192954798489E-5 0.0 6.195150715119145E-4 2.114385909596978E-6 82-83 0.2201054588031358 1.057192954798489E-5 0.0 6.438305094722797E-4 2.114385909596978E-6 84-85 0.26937805084742894 1.057192954798489E-5 0.0 6.480592812914737E-4 3.171578864395467E-6 86-87 0.367568018103203 1.057192954798489E-5 0.0 6.533452460654661E-4 4.228771819193956E-6 88-89 0.5099835382370623 1.057192954798489E-5 0.0 6.55459631975063E-4 4.228771819193956E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 62840 0.0 63.1798 1 CGGTCGT 785 0.0 53.83646 1 CCCGAAA 10550 0.0 51.355785 1 GCGTGTT 79635 0.0 50.046585 2 CCGAAAC 13855 0.0 38.86581 2 TATGCCG 18265 0.0 38.18303 46-47 TGCCGTC 19030 0.0 36.437366 48-49 CGTGTTT 116225 0.0 34.69034 3 CGAAACC 15520 0.0 34.41994 3 CGGCCTT 4505 0.0 33.940422 1 CCGTCTT 20530 0.0 33.915882 50-51 TCGTCTA 11945 0.0 33.277737 44-45 CGAGCTA 12190 0.0 33.10862 12-13 ACTCGTC 12195 0.0 32.515415 42-43 TCACGGC 9705 0.0 32.392506 30-31 CTAACGA 11325 0.0 32.36829 94-95 CTCGTAT 21945 0.0 31.117733 42-43 ATCTCGT 21475 0.0 31.056158 40-41 CGTCTGA 23055 0.0 30.582254 16-17 CCAAACG 17625 0.0 30.523119 8 >>END_MODULE