##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233046_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38869220 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.760566792953394 33.0 31.0 34.0 30.0 34.0 2 32.01462118354832 33.0 31.0 34.0 30.0 34.0 3 32.03793106730724 34.0 31.0 34.0 30.0 34.0 4 35.48118503535702 37.0 35.0 37.0 33.0 37.0 5 35.42479833657583 37.0 35.0 37.0 33.0 37.0 6 35.447874024742454 37.0 35.0 37.0 33.0 37.0 7 35.56523938478827 37.0 35.0 37.0 33.0 37.0 8 35.59507178173372 37.0 35.0 37.0 33.0 37.0 9 37.21446478730471 39.0 37.0 39.0 34.0 39.0 10-11 37.12096836520003 39.0 37.0 39.0 33.5 39.0 12-13 37.1044411619271 39.0 37.0 39.0 33.0 39.0 14-15 38.41700780206034 40.0 38.0 41.0 33.5 41.0 16-17 38.15919830652635 40.0 38.0 41.0 33.0 41.0 18-19 37.762832583211086 40.0 37.0 41.0 33.0 41.0 20-21 37.35277603975588 40.0 36.5 41.0 32.0 41.0 22-23 36.939925781891176 40.0 36.0 41.0 31.5 41.0 24-25 36.570781186244545 40.0 35.5 41.0 30.5 41.0 26-27 36.050150800556324 40.0 35.0 41.0 28.0 41.0 28-29 35.5234959564406 40.0 35.0 41.0 25.0 41.0 30-31 35.12456453975665 40.0 35.0 41.0 22.0 41.0 32-33 34.82803075286821 40.0 35.0 41.0 18.5 41.0 34-35 34.56791421335443 39.5 35.0 41.0 15.5 41.0 36-37 34.41259303634084 39.0 35.0 41.0 12.0 41.0 38-39 34.22539973789029 39.0 35.0 41.0 9.0 41.0 40-41 34.074563729346764 39.0 35.0 41.0 8.0 41.0 42-43 34.093783152839194 39.0 35.0 41.0 7.5 41.0 44-45 33.96967524689201 39.0 35.0 41.0 7.0 41.0 46-47 33.8355207539539 39.0 35.0 41.0 3.5 41.0 48-49 33.68607684949685 39.0 34.0 41.0 2.0 41.0 50-51 33.220264106148775 38.5 33.5 40.5 2.0 41.0 52-53 33.20991064394912 38.5 33.5 40.0 2.0 41.0 54-55 33.3239993753412 39.0 34.0 41.0 2.0 41.0 56-57 33.20372515836438 39.0 34.0 41.0 2.0 41.0 58-59 33.02954763434924 38.5 34.0 41.0 2.0 41.0 60-61 32.83705299463174 38.0 33.0 40.5 2.0 41.0 62-63 32.59804845582185 38.0 33.0 40.0 2.0 41.0 64-65 32.35488533086077 38.0 33.0 40.0 2.0 41.0 66-67 32.082299940672854 37.5 32.5 40.0 2.0 41.0 68-69 31.818356452740755 37.0 32.0 40.0 2.0 41.0 70-71 31.493862264280065 36.5 31.5 40.0 2.0 41.0 72-73 31.1529191349865 36.0 31.0 39.0 2.0 41.0 74-75 30.797594021696344 36.0 31.0 39.0 2.0 40.5 76-77 30.40073791035683 35.0 31.0 38.5 2.0 40.0 78-79 30.00605297713718 35.0 30.0 37.5 2.0 39.5 80-81 29.62007632260179 35.0 30.0 37.0 2.0 39.0 82-83 29.298614868011242 35.0 29.5 37.0 2.0 39.0 84-85 28.91285284345814 35.0 29.0 36.0 2.0 38.5 86-87 28.55160696561444 35.0 29.0 36.0 2.0 37.0 88-89 28.200105507648466 34.0 29.0 35.5 2.0 37.0 90-91 27.87494289826243 34.0 29.0 35.0 2.0 36.5 92-93 27.47746310576852 34.0 27.0 35.0 2.0 36.0 94-95 27.11512511699489 34.0 26.0 35.0 2.0 36.0 96-97 26.69410722674651 34.0 25.5 35.0 2.0 35.5 98-99 26.21943023297097 34.0 24.5 35.0 2.0 35.0 100-101 23.98502267346759 31.5 10.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31188953363406213 1101 2 0.2547240980656653 1101 3 0.26728181894026193 1101 4 0.3013469343667978 1101 5 0.30304092966368046 1101 6 0.29991490329945236 1101 7 0.2751266491350606 1101 8 0.2383099524025809 1101 9 0.36992392182771994 1101 10-11 0.42046927693755265 1101 12-13 0.38232869123820734 1101 14-15 0.4386126234829604 1101 16-17 0.42112466734617726 1101 18-19 0.41568807272705754 1101 20-21 0.3643690878050947 1101 22-23 0.42984184617604626 1101 24-25 0.4158477826489104 1101 26-27 0.4181137757933513 1101 28-29 0.3664364672760456 1101 30-31 0.33810257977409464 1101 32-33 0.2899383035400973 1101 34-35 0.3003395013578398 1101 36-37 0.30337261601524546 1101 38-39 0.3535564863070704 1101 40-41 0.44151475912685356 1101 42-43 0.3944032745391439 1101 44-45 0.37706918452690985 1101 46-47 0.3313120723872274 1101 48-49 0.33301337356445515 1101 50-51 0.3499930380727321 1101 52-53 0.3710235907054198 1101 54-55 0.3868650630858923 1101 56-57 0.3911760889649756 1101 58-59 0.3860968516135159 1101 60-61 0.33235423296449795 1101 62-63 0.32770571202538434 1101 64-65 0.32933134953923826 1101 66-67 0.2852514494017484 1101 68-69 0.3268416856651264 1101 70-71 0.2600603255955747 1101 72-73 0.2705015593288387 1101 74-75 0.24793081322938093 1101 76-77 0.17998688991713152 1101 78-79 0.3160674995061328 1101 80-81 0.2582975483886294 1101 82-83 0.2411536120700113 1101 84-85 0.2484587138405736 1101 86-87 0.23134190296221036 1101 88-89 0.1395926667680918 1101 90-91 0.11308385477108729 1101 92-93 0.044825421379098174 1101 94-95 0.09269233092734552 1101 96-97 -2.3228893333993028E-4 1101 98-99 0.040215927358826065 1101 100-101 0.1975059154482217 1102 1 0.19116500586308405 1102 2 0.17118327159813873 1102 3 0.18251524824996324 1102 4 0.1766812076502049 1102 5 0.19008810474376503 1102 6 0.2153468832938117 1102 7 0.16434942531154917 1102 8 0.1602019026423207 1102 9 0.27012341601706424 1102 10-11 0.2629998949258763 1102 12-13 0.25663449505800173 1102 14-15 0.2751393262779587 1102 16-17 0.27152666177462237 1102 18-19 0.3095243750172685 1102 20-21 0.23800545640832382 1102 22-23 0.24325115072511494 1102 24-25 0.2653461003691078 1102 26-27 0.22527397900319102 1102 28-29 0.15409507288014623 1102 30-31 0.17495960465240046 1102 32-33 0.17389971696363915 1102 34-35 0.10511035797297552 1102 36-37 0.12212673082014192 1102 38-39 0.13741686244815554 1102 40-41 0.23415431240943008 1102 42-43 0.1596201168172584 1102 44-45 0.1355548373644524 1102 46-47 0.08170053191201276 1102 48-49 0.0853222403076046 1102 50-51 0.13186030557194783 1102 52-53 0.07469988206326406 1102 54-55 0.11647677075892204 1102 56-57 0.10301255805803322 1102 58-59 0.1044999760972587 1102 60-61 0.0313229790650027 1102 62-63 0.056491949653711515 1102 64-65 0.07795970328968593 1102 66-67 0.10568444410247935 1102 68-69 0.1260413808648444 1102 70-71 0.12030982421609338 1102 72-73 0.05046602365153774 1102 74-75 0.04985405425306766 1102 76-77 0.06280284588012464 1102 78-79 0.10111885720826308 1102 80-81 0.0742185139423519 1102 82-83 0.026601611457216734 1102 84-85 0.02656254635436639 1102 86-87 0.009566476646757138 1102 88-89 -0.05789352630267075 1102 90-91 -0.05845378752175989 1102 92-93 -0.08525967659870304 1102 94-95 -0.05013449267354986 1102 96-97 -0.08696101802091505 1102 98-99 -0.31013322532241006 1102 100-101 -0.12499135447712462 1103 1 0.1338397744439952 1103 2 0.14369882311891047 1103 3 0.20241481824216834 1103 4 0.19292335864984267 1103 5 0.1702688365268159 1103 6 0.20209558712107878 1103 7 0.23064674576331612 1103 8 0.18771575198920232 1103 9 0.23356294416564793 1103 10-11 0.28285097970601925 1103 12-13 0.2504951499079695 1103 14-15 0.275782006171589 1103 16-17 0.29327110999235373 1103 18-19 0.27485608847116794 1103 20-21 0.30591106025565296 1103 22-23 0.3305131496527025 1103 24-25 0.3235034847169018 1103 26-27 0.3375812800881448 1103 28-29 0.3482992808889378 1103 30-31 0.2948856633930248 1103 32-33 0.21984832634455387 1103 34-35 0.24998270332109485 1103 36-37 0.25688261866726947 1103 38-39 0.1760742873838268 1103 40-41 0.2467645717810143 1103 42-43 0.2328411073726997 1103 44-45 0.24573333733847846 1103 46-47 0.2723471335527634 1103 48-49 0.26896870861315847 1103 50-51 0.2898702975319907 1103 52-53 0.3531788909275022 1103 54-55 0.3351221682575982 1103 56-57 0.3423504854779509 1103 58-59 0.36645733693833904 1103 60-61 0.3118138567903159 1103 62-63 0.23703141397339067 1103 64-65 0.21479054775741702 1103 66-67 0.27971975478924804 1103 68-69 0.2506824822709852 1103 70-71 0.30788770942182353 1103 72-73 0.2339021306718152 1103 74-75 0.3155723908645456 1103 76-77 0.272199992450755 1103 78-79 0.30942146646010116 1103 80-81 0.2940520735679719 1103 82-83 0.2895843039600088 1103 84-85 0.20585450144494288 1103 86-87 0.1899573704464146 1103 88-89 0.10931326681499343 1103 90-91 0.14951262752801497 1103 92-93 0.14501561690481068 1103 94-95 0.21062738204323495 1103 96-97 0.1927763200112409 1103 98-99 0.11094847280178399 1103 100-101 0.04019463764904074 1104 1 0.20674565514288545 1104 2 0.14666498484763224 1104 3 0.14674811742683147 1104 4 0.21644612300099908 1104 5 0.18435291392776065 1104 6 0.12903267092313087 1104 7 0.16894464193138248 1104 8 0.1692515727091788 1104 9 0.2354033664616395 1104 10-11 0.26003175010043833 1104 12-13 0.26836269766686627 1104 14-15 0.2800604261609152 1104 16-17 0.25878682072902137 1104 18-19 0.2715659180809169 1104 20-21 0.2866836608970189 1104 22-23 0.3387663487537935 1104 24-25 0.3092104175864634 1104 26-27 0.2775961128842468 1104 28-29 0.271378099518941 1104 30-31 0.26989898364698917 1104 32-33 0.28097474843415426 1104 34-35 0.2646808717487801 1104 36-37 0.20711820413584547 1104 38-39 0.20738753713840197 1104 40-41 0.29512148744747435 1104 42-43 0.2155664821049541 1104 44-45 0.22546352928532087 1104 46-47 0.15815149357717928 1104 48-49 0.24314529565182852 1104 50-51 0.21971313294946526 1104 52-53 0.30120756863438203 1104 54-55 0.35353147281916364 1104 56-57 0.2683635361624894 1104 58-59 0.2798821898851003 1104 60-61 0.3113169624279877 1104 62-63 0.24324697830444109 1104 64-65 0.2521935363252652 1104 66-67 0.2441523171935529 1104 68-69 0.26111778313053335 1104 70-71 0.19617226753014094 1104 72-73 0.2385442007674463 1104 74-75 0.2696099602992241 1104 76-77 0.1891452737652486 1104 78-79 0.29803194438428093 1104 80-81 0.2512070647118527 1104 82-83 0.2557732348376156 1104 84-85 0.2549088032647866 1104 86-87 0.24936641228954315 1104 88-89 0.17069133383530755 1104 90-91 0.21484700312499783 1104 92-93 0.20828317794919826 1104 94-95 0.21061726937439573 1104 96-97 0.2128944916187372 1104 98-99 0.11932639515772081 1104 100-101 -0.06576381554464916 1105 1 0.12112519907489983 1105 2 0.08468103188582887 1105 3 0.10266204584203109 1105 4 0.11431067107600512 1105 5 0.14510067422160233 1105 6 0.16206091791699606 1105 7 0.15702911799500896 1105 8 0.15436386717532002 1105 9 0.15929557355134705 1105 10-11 0.20157244945423258 1105 12-13 0.16384112496019299 1105 14-15 0.20240232235207856 1105 16-17 0.23506680820192827 1105 18-19 0.1933087654545531 1105 20-21 0.1418824031130157 1105 22-23 0.18797265904069604 1105 24-25 0.16941997067291936 1105 26-27 0.18744118842559487 1105 28-29 0.12388649596350376 1105 30-31 0.17986218351828853 1105 32-33 0.1468571151158713 1105 34-35 0.08232142553616484 1105 36-37 0.05358466433436604 1105 38-39 0.04974297506820591 1105 40-41 0.16101709198161274 1105 42-43 0.11483364120109485 1105 44-45 0.10158075892956475 1105 46-47 0.02253573851366042 1105 48-49 0.09304123198769787 1105 50-51 0.1071803134717868 1105 52-53 0.07283557488565862 1105 54-55 0.12154754839962578 1105 56-57 0.12459166326867432 1105 58-59 0.1480287967884948 1105 60-61 0.09909803250398852 1105 62-63 0.02138008471573727 1105 64-65 0.05569554285084166 1105 66-67 0.04730060711668216 1105 68-69 0.046556320785374083 1105 70-71 -0.02005962037961595 1105 72-73 0.01998173939214709 1105 74-75 0.0654561093855115 1105 76-77 0.022270718610656104 1105 78-79 0.03516688826540104 1105 80-81 0.02653615175205104 1105 82-83 0.01753260843768345 1105 84-85 0.0061524224272382355 1105 86-87 0.0590445926439962 1105 88-89 0.024835316493263093 1105 90-91 -0.002289776269474686 1105 92-93 0.0363510263216007 1105 94-95 -0.02625319695228967 1105 96-97 -0.01722610979036432 1105 98-99 -0.07503777169169723 1105 100-101 -0.19080479635314163 1106 1 0.05576397096528041 1106 2 0.017508613626880276 1106 3 0.02893736506845812 1106 4 0.05028280478476432 1106 5 0.08617002055501644 1106 6 0.052467828811536776 1106 7 0.08776414194441884 1106 8 0.06657350771590842 1106 9 0.10006851445167797 1106 10-11 0.0679418077458891 1106 12-13 0.12083071549422897 1106 14-15 0.0783010277885623 1106 16-17 0.08875823737576383 1106 18-19 0.06075579958704935 1106 20-21 0.05189683983363125 1106 22-23 0.06664314632038781 1106 24-25 0.011694945775829524 1106 26-27 0.03741987259478208 1106 28-29 0.019698166983673104 1106 30-31 -0.04105332070323442 1106 32-33 -0.09242672463377488 1106 34-35 -0.056447854921231055 1106 36-37 -0.09482689998904448 1106 38-39 -0.0742293347994547 1106 40-41 -0.022768877750152683 1106 42-43 -0.12074728804445556 1106 44-45 -0.12125983574610188 1106 46-47 -0.05223876339089628 1106 48-49 -0.06181186673759953 1106 50-51 -0.0984470301157927 1106 52-53 -0.03881302355502214 1106 54-55 -0.004924469667443532 1106 56-57 0.007554075834526941 1106 58-59 0.018562192616741413 1106 60-61 -0.013418002732251466 1106 62-63 0.0034861901067486656 1106 64-65 -0.016539703763946534 1106 66-67 -0.07171892343129116 1106 68-69 -0.02171054941860362 1106 70-71 -0.041028195953931146 1106 72-73 -0.02660212436070708 1106 74-75 -0.045994912718811065 1106 76-77 -0.08406345037991514 1106 78-79 -0.04231367558470822 1106 80-81 -0.05782030749119471 1106 82-83 -0.12817040603656338 1106 84-85 -0.06159422237982426 1106 86-87 0.0047255572715521055 1106 88-89 -0.10333103751182549 1106 90-91 -0.07898742092002209 1106 92-93 -0.12406387979686073 1106 94-95 -0.08722124347842097 1106 96-97 -0.18168358457912603 1106 98-99 -0.2158165197852 1106 100-101 -0.13831472822070978 1107 1 -0.019671656960618122 1107 2 -0.054447316756068176 1107 3 -0.029637738260703372 1107 4 -0.0016934788307096937 1107 5 -0.004077708562640225 1107 6 0.05278124503383452 1107 7 0.05757505100714155 1107 8 0.023063847861429565 1107 9 0.07654459735979913 1107 10-11 0.0914458309557844 1107 12-13 0.06681741063103885 1107 14-15 0.007440464608329478 1107 16-17 -0.009078559173737233 1107 18-19 -0.007408671308162695 1107 20-21 -0.07867797102765905 1107 22-23 0.008369048634804699 1107 24-25 -0.06961948253321992 1107 26-27 -0.007688765382987128 1107 28-29 -0.03989209848549535 1107 30-31 -0.08042557422076868 1107 32-33 -0.10336741425481222 1107 34-35 -0.06373119694735863 1107 36-37 -0.05471239173935061 1107 38-39 -0.020283369756228353 1107 40-41 0.009477788745428484 1107 42-43 -0.04436141816992034 1107 44-45 -0.036524463559146625 1107 46-47 -0.040494175341692085 1107 48-49 -0.014159107919638814 1107 50-51 -0.05469849270578209 1107 52-53 -0.02085858493168047 1107 54-55 -0.009814139173762726 1107 56-57 0.019999290045603857 1107 58-59 -0.005495423660818233 1107 60-61 -0.016045840623164054 1107 62-63 0.024464096005367253 1107 64-65 -0.02693536257323359 1107 66-67 0.04532344391905241 1107 68-69 0.049119464420577685 1107 70-71 -0.02838610698107047 1107 72-73 -0.014479901129622164 1107 74-75 -0.1516103220449594 1107 76-77 -0.05625197824262784 1107 78-79 0.015032854479500202 1107 80-81 0.007742700688805115 1107 82-83 -0.0614533941522204 1107 84-85 -0.03320219268465863 1107 86-87 -0.06555123306019439 1107 88-89 -0.11555838430778564 1107 90-91 -0.07719453267744925 1107 92-93 -0.1385434065073916 1107 94-95 -0.04330148951218149 1107 96-97 -0.1504345902317965 1107 98-99 -0.17951672242405436 1107 100-101 -0.18293563354404796 1108 1 -0.10575553527358394 1108 2 -0.16751978029397208 1108 3 -0.1990503669225241 1108 4 -0.11000449351838171 1108 5 -0.10859208677645427 1108 6 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2316 46-47 -0.57307513100384 2316 48-49 -0.47141188915665566 2316 50-51 -0.5975619921763169 2316 52-53 -0.5175786502644328 2316 54-55 -0.4473015741273798 2316 56-57 -0.4607381466827789 2316 58-59 -0.37739663336875395 2316 60-61 -0.627298093630337 2316 62-63 -0.45809024266505105 2316 64-65 -0.4711114125545919 2316 66-67 -0.45438597645121703 2316 68-69 -0.5014510283980336 2316 70-71 -0.40461293732765924 2316 72-73 -0.4063708362043492 2316 74-75 -0.36870220360376393 2316 76-77 -0.44949029711847643 2316 78-79 -0.40802808405792135 2316 80-81 -0.4041561583431026 2316 82-83 -0.28299704131424974 2316 84-85 -0.40112923196475236 2316 86-87 -0.41322997374276227 2316 88-89 -0.36371369776346896 2316 90-91 -0.434400470794305 2316 92-93 -0.470308138987118 2316 94-95 -0.47144360480765357 2316 96-97 -0.4405146122866981 2316 98-99 -0.38641932579194815 2316 100-101 -0.46216051472544706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 139152.0 3 31142.0 4 26146.0 5 35759.0 6 74677.0 7 160875.0 8 301682.0 9 513999.0 10 713760.0 11 706480.0 12 538901.0 13 373761.0 14 280030.0 15 236958.0 16 211084.0 17 200162.0 18 193388.0 19 197115.0 20 207920.0 21 220336.0 22 238613.0 23 264573.0 24 291267.0 25 318811.0 26 356715.0 27 410272.0 28 477561.0 29 567662.0 30 682886.0 31 836661.0 32 1044674.0 33 1342170.0 34 1825437.0 35 2714811.0 36 3632994.0 37 6112845.0 38 9187304.0 39 3197462.0 40 3175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.06732875411252 16.980136347046116 9.684246371894984 41.26828852694638 2 17.150242295077263 17.708097014355143 51.51438989941578 13.627270791151815 3 21.532055535501613 20.285071881595343 35.66045468965115 22.5224178932519 4 22.48103886826131 25.607268330895423 34.50433934043672 17.40735346040654 5 19.528534814945175 29.585426757275137 37.52418103206146 13.36185739571823 6 16.426260192612332 32.29924224822149 38.62969133169367 12.644806227472507 7 11.916864211595353 20.347954913054114 55.66524941737465 12.069931457975878 8 12.277992715058343 22.528460823242657 50.47362154424504 14.719924917453964 9 13.438646311914674 21.128924120422276 50.48121109710975 14.951218470553307 10-11 14.972468124810174 31.572226450289282 40.80411622363552 12.651189201265026 12-13 14.64789665100186 25.944356213326436 45.54232238146759 13.865424754204112 14-15 14.906046480994473 27.589016450548787 43.82941309344515 13.675523975011592 16-17 15.955311140351633 28.126893289145816 41.708595971694294 14.209199598808258 18-19 16.276039658395312 28.810704157192852 40.48144935330862 14.431806831103215 20-21 16.677740456070147 29.03684840836907 39.568036281516115 14.717374854044667 22-23 16.555118129987818 29.412847615918885 39.52638250416191 14.505651749931392 24-25 16.44404088465325 29.89564226310489 38.46945931088508 15.190857541356785 26-27 16.195639959582156 30.752120126080307 37.90699818011644 15.145241734221099 28-29 16.838088527435183 30.836465803683822 36.71764822835634 15.607797440524656 30-31 16.821441233692095 31.18948885460131 36.36081167368646 15.628258238020132 32-33 16.739248315452564 31.27118881831522 36.28827391458418 15.701288951648037 34-35 16.569610094759938 32.00452834761429 35.68067006474976 15.74519149287601 36-37 16.709359843160172 31.845363768529484 35.72456156748649 15.720714820823856 38-39 16.58165152234713 31.26303164405998 36.11092737130287 16.044389462290017 40-41 16.684489360738432 31.37712702932072 36.269198762704086 15.669184847236759 42-43 16.549877864422914 31.305234614348805 36.400383429234594 15.744504091993687 44-45 16.885784817989506 30.740488808837835 36.366697498809316 16.007028874363343 46-47 16.29369631596937 31.360225054908504 36.071414250336 16.274664378786134 48-49 16.068761499870952 31.570067171737946 35.9194906566238 16.441680671767294 50-51 16.358924131668783 31.846373897253947 35.725199706388985 16.069502264688285 52-53 16.18626522791555 31.982177030852277 35.73319735374437 16.098360387487798 54-55 16.40751024528317 32.248698977558384 35.155193584224776 16.188597192933667 56-57 16.587143985177462 32.3957557147926 34.82538850743037 16.191711792599566 58-59 16.43369232344721 33.10278150439295 34.54486064174629 15.918665530413554 60-61 16.224205105562227 33.62773734647856 33.92806453638148 16.219993011577728 62-63 16.11934569964824 34.10217437484456 33.455976156541915 16.32250376896529 64-65 16.17546047561151 33.42589547750736 33.742386686880366 16.656257360000765 66-67 16.398925674026387 33.57305727642314 33.851466130369275 16.1765509191812 68-69 16.61916446703883 33.31555180027053 33.9488855830852 16.116398149605434 70-71 16.264444761122583 33.57484036803718 33.60298375475877 16.557731116081477 72-73 16.503861884031725 33.39723441152434 33.56534813199594 16.533555572447987 74-75 16.793973255972766 33.43189711957162 33.278239619057445 16.495890005398163 76-77 16.36843784418335 33.42083515528734 33.57935399901436 16.631373001514945 78-79 16.306737968437442 33.89208206622685 33.29685542255732 16.50432454277839 80-81 16.31269852448613 34.37843545291489 33.09271198916337 16.216154033435608 82-83 16.593820131703968 34.140034981163666 32.817073554965134 16.449071332167225 84-85 16.245012320098816 34.21166366201049 33.09588460163383 16.447439416256866 86-87 16.293672403795696 34.46314270251602 32.97754437093282 16.26564052275546 88-89 16.46883809726863 34.18570053017555 32.95411950632601 16.39134186622982 90-91 16.33876573168728 34.55382126706578 32.47097245312035 16.63644054812659 92-93 16.895850379377407 34.24535965982614 32.43120914763773 16.427580813158723 94-95 17.174267118166437 34.861394622747085 31.54697534607583 16.417362913010646 96-97 16.992181831527176 35.17501150381745 31.625713316799516 16.207093347855864 98-99 17.293908661149437 34.75533464638557 31.59763669131378 16.353120001151208 100-101 17.758446309550813 35.331239995431076 30.499996299817727 16.41031739520038 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 263012.0 1 202033.5 2 127321.0 3 131401.0 4 152531.5 5 183459.0 6 194745.5 7 181840.5 8 189954.5 9 201445.5 10 214435.5 11 224677.5 12 238664.5 13 250439.5 14 280142.5 15 303024.0 16 297386.0 17 303288.5 18 320421.0 19 345242.0 20 382929.0 21 437018.0 22 510044.0 23 586943.0 24 675634.5 25 827467.0 26 998055.5 27 1142963.5 28 1295668.0 29 1457488.5 30 1590977.0 31 1650681.0 32 1632431.0 33 1621784.0 34 1595439.0 35 1535445.0 36 1511856.0 37 1458657.0 38 1399767.0 39 1339942.5 40 1260537.5 41 1173624.0 42 1015053.0 43 856386.0 44 756585.0 45 692147.5 46 647877.0 47 604503.5 48 563386.5 49 508198.0 50 439522.5 51 378091.0 52 326979.0 53 280504.5 54 236530.0 55 198679.5 56 169352.0 57 143025.5 58 118790.0 59 97871.5 60 75943.5 61 55496.5 62 41256.5 63 31094.5 64 22562.5 65 15538.0 66 10218.0 67 6964.5 68 4642.0 69 2973.5 70 2042.5 71 1550.0 72 1263.0 73 1083.5 74 905.0 75 679.5 76 542.0 77 425.5 78 320.5 79 232.5 80 165.0 81 129.5 82 92.0 83 56.5 84 45.5 85 45.5 86 29.5 87 21.0 88 22.0 89 17.0 90 13.5 91 12.5 92 11.5 93 9.5 94 7.0 95 3.0 96 3.0 97 4.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0472353188461204 2 0.0012812194327542462 3 0.004708095505904157 4 0.027734027078495527 5 0.06626837379293951 6 0.01297170357419058 7 0.015202260297479599 8 0.0 9 0.0 10-11 1.2863648923235403E-6 12-13 7.33227988624418E-5 14-15 0.0 16-17 6.431824461617702E-5 18-19 5.788642015455931E-5 20-21 0.0029946574693292018 22-23 0.0016439743323894843 24-25 0.008846331364508986 26-27 0.007004256838701676 28-29 1.0290919138588323E-5 30-31 0.0023553341178444024 32-33 0.0017867608354373973 34-35 0.001897388216177222 36-37 2.8300027631117887E-5 38-39 0.0021893930467346656 40-41 0.20029473192412917 42-43 0.003994162990664593 44-45 0.00178032901097578 46-47 0.007644866555078799 48-49 0.007777362138988124 50-51 0.017798144650188506 52-53 0.004172967710697565 54-55 0.01033722827471197 56-57 0.008398676381980394 58-59 0.011937466200762454 60-61 0.012709285136156578 62-63 0.023373250093518726 64-65 0.01070126953923953 66-67 0.03451445642593291 68-69 0.024739369609166326 70-71 0.01019186904187941 72-73 0.006108946873644493 74-75 0.02255640838689328 76-77 0.010611223996776884 78-79 0.03888037887047901 80-81 0.08818031336877868 82-83 0.05372888882256963 84-85 0.08303485379948453 86-87 0.034632801996026676 88-89 0.013878590823278678 90-91 0.019774001124797463 92-93 0.027321103948059672 94-95 0.05685218278113119 96-97 0.05186494609359282 98-99 0.059400471632824116 100-101 0.050895026964780876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.886922E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.23688728957486 #Duplication Level Percentage of deduplicated Percentage of total 1 68.56241631368324 22.788013033187994 2 15.198959054555297 10.103321780302355 3 6.140553493569386 6.122786530841126 4 2.873336504099722 3.820030461271341 5 1.6085047272661346 2.6730845162446433 6 0.9919732813287371 1.9782062487475784 7 0.6782815488699643 1.5780777173272487 8 0.5184777549086591 1.378606936164074 9 0.41377775515125886 1.2377416149806126 >10 2.5442782709058602 16.193026044308553 >50 0.26384235257995714 6.093573448056993 >100 0.17910137990898403 11.489248544509472 >500 0.016125871318981955 3.6944116109334693 >1k 0.009367277549990168 5.798326111929188 >5k 6.490061738883257E-4 1.4266714792939275 >10k+ 3.554081299499941E-4 3.624873921901501 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 288565 0.7423997703066848 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138396 0.35605551127601737 No Hit CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81083 0.20860464912853924 No Hit CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74067 0.19055437695945532 No Hit CTCGAGATGATTGTCATAAGCTTCTTGCAATACAAATGTTTTCTCTCAAA 62816 0.161608594152391 No Hit GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57075 0.14683855245873212 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 41210 0.1060221944253062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.12010274453667967 0.0 0.0 5.1454595692941615E-5 0.0 2 0.1204706448958842 2.5727297846470806E-6 0.0 6.68909744008241E-5 0.0 3 0.12048865400437672 2.5727297846470806E-6 0.0 9.26182722472949E-5 0.0 4 0.12155119140543597 2.5727297846470806E-6 0.0 1.0805465095517738E-4 0.0 5 0.12176730070734633 2.5727297846470806E-6 0.0 1.1062738073982447E-4 0.0 6 0.12193452814334839 2.5727297846470806E-6 0.0 1.2606375944770694E-4 0.0 7 0.1231591475208404 2.5727297846470806E-6 0.0 1.6465470621741316E-4 0.0 8 0.137880307348591 2.5727297846470806E-6 0.0 1.852365444945898E-4 0.0 9 0.13799350745911546 2.5727297846470806E-6 0.0 2.0324565298711938E-4 0.0 10-11 0.14054822813527001 2.5727297846470806E-6 0.0 2.1225020723338414E-4 0.0 12-13 0.14263213926083415 2.5727297846470806E-6 0.0 2.302593157259137E-4 0.0 14-15 0.14407415430512882 1.8009108492529566E-5 0.0 2.4698205932611973E-4 0.0 16-17 0.144798377739507 1.8009108492529566E-5 0.0 2.559866135723845E-4 0.0 18-19 0.1456190785408094 1.8009108492529566E-5 0.0 3.0744120926532614E-4 0.0 20-21 0.14668290230676098 1.8009108492529566E-5 0.0 3.4345942625038525E-4 0.0 22-23 0.1479345353469918 2.0581838277176645E-5 0.0 3.730458187738267E-4 0.0 24-25 0.14899835911294335 2.0581838277176645E-5 0.0 3.949140219433269E-4 0.0 26-27 0.1501702375298501 2.3154568061823725E-5 0.0 5.042550377908278E-4 0.0 28-29 0.1513241068382643 2.3154568061823725E-5 0.0 5.955869451457992E-4 0.0 30-31 0.15230817598089183 2.3154568061823725E-5 0.0 6.290324323462112E-4 0.0 32-33 0.1529963811982849 2.3154568061823725E-5 0.0 6.35464256807829E-4 0.0 34-35 0.15343374526167491 2.3154568061823725E-5 0.0 6.483279057310643E-4 0.0 36-37 0.15381193654001804 2.3154568061823725E-5 0.0 6.714824737928881E-4 0.0 38-39 0.15428660518528542 2.3154568061823725E-5 0.0 6.804870280391528E-4 0.0 40-41 0.15489762850913913 2.3154568061823725E-5 0.0 6.946370418547118E-4 0.0 42-43 0.155835388515643 3.0872757415764966E-5 0.0 7.16505245024212E-4 0.0 44-45 0.1567782939817161 3.0872757415764966E-5 0.0 7.306552588397709E-4 0.0 46-47 0.1579900497102849 3.344548720041205E-5 0.0 7.589552864708889E-4 0.0 48-49 0.15929442371110097 3.344548720041205E-5 0.0 7.743916651787713E-4 0.0 50-51 0.16094097077327513 3.601821698505913E-5 0.0 7.859689492096831E-4 0.0 52-53 0.16320111388908756 4.245004144667683E-5 0.0 7.911144087789773E-4 0.0 54-55 0.16540980240920708 4.759550101597099E-5 0.0 7.94973503455948E-4 0.0 56-57 0.16799025038320808 5.402732547758869E-5 0.0 8.16841706625448E-4 0.0 58-59 0.17118300804595513 6.0459149939206394E-5 0.0 8.284189906563599E-4 0.0 60-61 0.17461631594356666 6.560460950850056E-5 0.0 8.374235449026248E-4 0.0 62-63 0.17856931525767689 6.946370418547117E-5 0.0 8.605781129644484E-4 0.0 64-65 0.1823782417038469 6.946370418547117E-5 0.0 8.747281267800075E-4 0.0 66-67 0.19350529802244554 6.946370418547117E-5 0.0 8.888781405955663E-4 0.0 68-69 0.20821102147148823 7.203643397011826E-5 0.0 8.965963299495076E-4 0.0 70-71 0.2134002174471214 7.203643397011826E-5 0.0 9.146054384420372E-4 0.0 72-73 0.22085598836302864 7.460916375476534E-5 0.0 9.339009118268903E-4 0.0 74-75 0.23198947650608887 7.718189353941242E-5 0.0 9.416191011808316E-4 0.0 76-77 0.23941180193479572 7.718189353941242E-5 0.0 9.57055479888714E-4 0.0 78-79 0.2482246878121043 7.718189353941242E-5 0.0 9.802100479505377E-4 0.0 80-81 0.25820687937653497 7.718189353941242E-5 0.0 0.0010175146298279205 0.0 82-83 0.271168292031587 7.718189353941242E-5 0.0 0.0010432419276743912 0.0 84-85 0.3038818376082669 7.718189353941242E-5 0.0 0.0011320011052447154 0.0 86-87 0.3908092315719225 7.846825843173597E-5 0.0 0.0011770238764760394 0.0 88-89 0.5433836850855253 8.232735310870658E-5 0.0 0.0011860284307223042 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 96895 0.0 61.87776 1 GCGTGTT 111960 0.0 53.822426 2 CGTGTTT 195345 0.0 37.92383 1 CCCGAAA 7070 0.0 36.614277 1 CGCCGTA 9835 0.0 36.08585 46-47 CCGAAAC 7085 0.0 35.664734 2 GTCGCCG 10580 0.0 34.62012 44-45 CGCGCGT 1095 0.0 32.9665 1 CGAAACC 7825 0.0 32.352665 3 GAGCGGC 2890 0.0 32.027283 9 CCGTATC 11300 0.0 31.807707 48-49 TCGGTGG 13510 0.0 31.097618 38-39 CGGGTGT 2885 0.0 30.457851 1 CTCGAGA 28135 0.0 29.830671 1 TCTCGGT 12645 0.0 29.210167 36-37 CGGGCCT 2770 0.0 28.978792 1 GTGTTTT 367855 0.0 28.916637 1 TCGAGAT 28910 0.0 28.849855 2 GATCTCG 13770 0.0 28.709528 34-35 CGAGATG 28860 0.0 28.389647 3 >>END_MODULE