FastQCFastQC Report
Fri 27 May 2016
SRR1233046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38869220
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4369751.124218597646158No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1297140.33371907128571143No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC874050.2248694468270781TruSeq Adapter, Index 11 (100% over 50bp)
CGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT636960.16387259636288046No Hit
CTCGAGATGATTGTCATAAGCTTCTTGCAATACAAATGTTTTCTCTCAAA586230.1508211381653658No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT565890.14558820578339365No Hit
CGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555900.14301804872853122No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT427550.10999706194258593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT806950.069.660261
GCGTGTT934250.060.4421042
CGCGCGT9250.040.0411221
CCCGAAA69450.039.8611371
CGTGTTT1681550.039.183921
TATGCCG219000.039.10307346-47
TGCCGTC226900.037.60718548-49
CTCGTAT226200.037.22142842-43
CCGAAAC73650.036.8783152
CCGTCTT234250.036.28685450-51
CGGGCCT27050.034.7577251
ACGGCTA188850.033.23137332-33
ATCTCGT251350.032.93836640-41
CGAAACC81950.032.9105763
TCACGGC194750.032.02844630-31
AGTCACG202150.031.00340728-29
CGTCTGA284800.030.60031516-17
GAGCACA419950.030.4039
CGGGTGT25500.030.1667271
CACGTCT292500.029.78757314-15
Produced by FastQC (version 0.11.5)