FastQCFastQC Report
Fri 27 May 2016
SRR1233045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47186955
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT2155390.4567766663477225No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT1526170.32343049048195627No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1314280.2785261307918682No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1063190.22531439038607176No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1041710.22076228483062743No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1012790.21463347232301808No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA909110.19266129802187915No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT858490.18193375690378835No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT829060.1756968636776838No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC768320.16282466202788462No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA757700.16057404000745545No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT753010.1595801212432546No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG679310.14396139780581307No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT669490.14188031416733712No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT643890.1364550859448337No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG626960.1328672299367484No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA602040.1275861093389052No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA585930.12417203017232199No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC560290.11873832503072088No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC540350.11451258086053658No Hit
ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT500710.10611195403475387No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT486310.1030602631595957No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA478600.10142633700352141No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT476930.10107242563119404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT449550.063.7119831
GCGTGTT599000.047.7040252
CCCGAAA129550.045.7204861
GTTTTTT7151250.045.1650351
CGTGTTT793450.036.126983
CCGAAAC170850.034.528732
CGGCCTT51850.033.986541
CGCCGTA66650.033.69475646-47
GTCGCCG71200.033.64003844-45
CCCAGAT317300.033.5619661
TCGTCTA158750.031.1323844-45
CAAAACG497100.031.0333489
GATATAT297150.031.0097735
CGAGCTA161850.030.7940812-13
CGAAACC192150.030.5775933
CTCGTAT48100.029.7237241
ACTCGTC165650.029.70491642-43
CTAACGA157450.029.08987694-95
TCAACGG156100.029.05660816-17
CGGACCA156150.029.02114920-21
Produced by FastQC (version 0.11.5)