##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233045_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47186955 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825427027448583 33.0 31.0 34.0 30.0 34.0 2 32.10079484891534 33.0 31.0 34.0 30.0 34.0 3 32.130477798366094 34.0 31.0 34.0 30.0 34.0 4 35.57328903719259 37.0 35.0 37.0 33.0 37.0 5 35.50811401159494 37.0 35.0 37.0 33.0 37.0 6 35.52461664457899 37.0 35.0 37.0 33.0 37.0 7 35.63158139786727 37.0 35.0 37.0 33.0 37.0 8 35.62386151002963 37.0 35.0 37.0 33.0 37.0 9 37.22085165275021 39.0 37.0 39.0 34.0 39.0 10-11 37.11504445455317 39.0 37.0 39.0 33.0 39.0 12-13 37.08387488660796 39.0 37.0 39.0 33.0 39.0 14-15 38.33116725798475 40.0 38.0 41.0 33.0 41.0 16-17 38.05308468198467 40.0 38.0 41.0 33.0 41.0 18-19 37.62428673348386 40.0 37.0 41.0 32.5 41.0 20-21 37.351856122099846 40.0 37.0 41.0 32.0 41.0 22-23 37.06173706271151 40.0 36.0 41.0 31.5 41.0 24-25 36.7661966638873 40.0 36.0 41.0 31.0 41.0 26-27 36.30341008441846 40.0 36.0 41.0 29.0 41.0 28-29 35.872287309066664 40.0 36.0 41.0 26.5 41.0 30-31 35.54512179266494 40.0 36.0 41.0 24.5 41.0 32-33 35.298190718176244 40.0 36.0 41.0 23.5 41.0 34-35 35.08214962164861 40.0 36.0 41.0 21.0 41.0 36-37 34.94139946093152 40.0 36.0 41.0 19.0 41.0 38-39 34.77927297915282 40.0 35.0 41.0 18.0 41.0 40-41 34.70953266639053 40.0 35.0 41.0 17.0 41.0 42-43 34.7702756302033 40.0 35.0 41.0 17.0 41.0 44-45 34.68419803736011 40.0 35.0 41.0 16.0 41.0 46-47 34.58018422676352 40.0 35.0 41.0 15.0 41.0 48-49 34.45822947253113 39.5 35.0 41.0 13.0 41.0 50-51 34.02101978184437 38.5 34.5 40.5 9.5 41.0 52-53 34.035246393839145 39.0 35.0 40.0 9.0 41.0 54-55 34.17134586243168 39.0 35.0 41.0 8.0 41.0 56-57 34.06010864655285 39.0 34.5 41.0 7.5 41.0 58-59 33.874906316798786 39.0 34.0 41.0 6.5 41.0 60-61 33.704487119374406 39.0 34.0 41.0 2.0 41.0 62-63 33.41639585559187 38.5 34.0 40.0 2.0 41.0 64-65 33.16645853711052 38.0 33.5 40.0 2.0 41.0 66-67 32.899474886650346 38.0 33.0 40.0 2.0 41.0 68-69 32.59354838641315 37.0 33.0 40.0 2.0 41.0 70-71 32.21174965623444 37.0 32.0 39.5 2.0 41.0 72-73 31.839017944684073 36.0 32.0 39.0 2.0 41.0 74-75 31.43887999342191 36.0 31.0 39.0 2.0 40.0 76-77 31.02136320302931 35.0 31.0 38.0 2.0 40.0 78-79 30.57602036410275 35.0 31.0 37.0 2.0 39.0 80-81 30.162200951512975 35.0 30.0 37.0 2.0 39.0 82-83 29.805104959622845 35.0 30.0 36.0 2.0 39.0 84-85 29.423970321882393 35.0 30.0 36.0 2.0 37.5 86-87 29.029625073285615 34.0 29.0 36.0 2.0 37.0 88-89 28.702647034545883 34.0 29.0 35.0 2.0 37.0 90-91 28.385623908133084 34.0 29.0 35.0 2.0 36.0 92-93 27.981783323378252 34.0 29.0 35.0 2.0 36.0 94-95 27.601346526810218 34.0 27.5 35.0 2.0 36.0 96-97 27.158297298480058 34.0 26.5 35.0 2.0 35.0 98-99 26.643752039096398 34.0 25.5 35.0 2.0 35.0 100-101 24.140833997446965 31.5 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35536541582780856 1101 2 0.2800508117488434 1101 3 0.2870866096653373 1101 4 0.2969138856822653 1101 5 0.2979353919536294 1101 6 0.30642602268886776 1101 7 0.2741946097102357 1101 8 0.26234220369615 1101 9 0.3851875587359004 1101 10-11 0.45200205756719214 1101 12-13 0.39751703168754204 1101 14-15 0.5019068070300321 1101 16-17 0.49478306498212277 1101 18-19 0.5074696350683823 1101 20-21 0.44283372073572735 1101 22-23 0.48060593131311435 1101 24-25 0.45268242588954877 1101 26-27 0.4706185868292465 1101 28-29 0.3908029577766001 1101 30-31 0.4368334705803747 1101 32-33 0.3861552028267994 1101 34-35 0.41180263933122063 1101 36-37 0.3593696899577026 1101 38-39 0.362749232271419 1101 40-41 0.41254746622228566 1101 42-43 0.4157040256183464 1101 44-45 0.40955702536987104 1101 46-47 0.36794852730205463 1101 48-49 0.3901716104751145 1101 50-51 0.3808225171978705 1101 52-53 0.42090103846627613 1101 54-55 0.43151870665188596 1101 56-57 0.45487226945211745 1101 58-59 0.4715830416185085 1101 60-61 0.40640856072326415 1101 62-63 0.3913829710571335 1101 64-65 0.40618434769033485 1101 66-67 0.3645109911507589 1101 68-69 0.34791104738386025 1101 70-71 0.2923382135206296 1101 72-73 0.32495788093883604 1101 74-75 0.2941225123256963 1101 76-77 0.25638240943945334 1101 78-79 0.33612291842423403 1101 80-81 0.2810577828214136 1101 82-83 0.2885797005567241 1101 84-85 0.2944197291816977 1101 86-87 0.274713738103614 1101 88-89 0.21205740749864788 1101 90-91 0.14793997369601897 1101 92-93 0.0503626095332379 1101 94-95 0.14785261621825185 1101 96-97 0.0999890963473753 1101 98-99 0.13336383275993668 1101 100-101 0.3695432065441331 1102 1 0.23967870984038697 1102 2 0.22213583441514118 1102 3 0.2395049937703675 1102 4 0.18853135812812383 1102 5 0.19119685397772912 1102 6 0.2467257778982983 1102 7 0.18854145962726676 1102 8 0.1825264414924277 1102 9 0.31609887963714556 1102 10-11 0.2906084176603727 1102 12-13 0.2885937945837682 1102 14-15 0.34964867508595177 1102 16-17 0.2956639583330798 1102 18-19 0.3172350170529157 1102 20-21 0.26147284751125 1102 22-23 0.2889208406813921 1102 24-25 0.27258320587674945 1102 26-27 0.27197672131598694 1102 28-29 0.17088767856048293 1102 30-31 0.1739386973279764 1102 32-33 0.18471610843300112 1102 34-35 0.1293928622548819 1102 36-37 0.16254736117094382 1102 38-39 0.16278672135807426 1102 40-41 0.21392739672633354 1102 42-43 0.14844681051611985 1102 44-45 0.11855961341883159 1102 46-47 0.052298342355548755 1102 48-49 0.06533120258439595 1102 50-51 0.08689136208406012 1102 52-53 0.05303612365245414 1102 54-55 0.12049129550683801 1102 56-57 0.0678307406094305 1102 58-59 0.07788762396419457 1102 60-61 0.047282589599959124 1102 62-63 0.012374402662821637 1102 64-65 0.040883012932290796 1102 66-67 0.07929846158579323 1102 68-69 0.14122201335080575 1102 70-71 0.10017737991377373 1102 72-73 0.007068804785451022 1102 74-75 0.009935764361376442 1102 76-77 0.02211495071306757 1102 78-79 0.04197171152751977 1102 80-81 0.0336390541101288 1102 82-83 -0.040688012530157636 1102 84-85 -0.017139230351912005 1102 86-87 -0.006027847349663773 1102 88-89 -0.11504031128912118 1102 90-91 -0.1696039047877136 1102 92-93 -0.18245922356722843 1102 94-95 -0.13723856323601424 1102 96-97 -0.12930635576873328 1102 98-99 -0.3362490772032274 1102 100-101 -0.09858883191296286 1103 1 0.19109749961585365 1103 2 0.2052489805368367 1103 3 0.23427510991999867 1103 4 0.257906082456536 1103 5 0.22326927421675435 1103 6 0.24153790114712592 1103 7 0.24147040461897262 1103 8 0.24637723227606756 1103 9 0.25901123182466534 1103 10-11 0.3211160734541991 1103 12-13 0.2855482798917848 1103 14-15 0.3469077118631745 1103 16-17 0.3296266980360869 1103 18-19 0.2915791812166546 1103 20-21 0.28657208928311917 1103 22-23 0.2966291433845569 1103 24-25 0.251915713070467 1103 26-27 0.29100593725348745 1103 28-29 0.29040837755861304 1103 30-31 0.22408467199769433 1103 32-33 0.16319797186792329 1103 34-35 0.17793206538021167 1103 36-37 0.19014602534518588 1103 38-39 0.11936188256031244 1103 40-41 0.19638943281042742 1103 42-43 0.1669299341640169 1103 44-45 0.19511755171040335 1103 46-47 0.24239096800928195 1103 48-49 0.26830950591765657 1103 50-51 0.2879163353045442 1103 52-53 0.32211259757655597 1103 54-55 0.3249398473120948 1103 56-57 0.28720231440539834 1103 58-59 0.3249523779076142 1103 60-61 0.3084197217753015 1103 62-63 0.2286059457936105 1103 64-65 0.185975533568282 1103 66-67 0.21038161463865634 1103 68-69 0.19079039040652646 1103 70-71 0.2217564267726928 1103 72-73 0.18615557044884312 1103 74-75 0.26717673808593645 1103 76-77 0.1802611774253826 1103 78-79 0.24499202195998038 1103 80-81 0.2620841613332523 1103 82-83 0.20968480047524096 1103 84-85 0.19416698039769997 1103 86-87 0.1856400445240638 1103 88-89 0.1243056381763381 1103 90-91 0.1478122385405065 1103 92-93 0.20049849628743033 1103 94-95 0.21069573766083494 1103 96-97 0.21062559273056536 1103 98-99 0.1833052274742606 1103 100-101 0.09957965856192885 1104 1 0.2736839739692165 1104 2 0.22335328511907449 1104 3 0.211639954661635 1104 4 0.27573743877855605 1104 5 0.23959477909915705 1104 6 0.24122634340118765 1104 7 0.2431172528104213 1104 8 0.2530355483962978 1104 9 0.3335730675443074 1104 10-11 0.3704079961216422 1104 12-13 0.34091731572407724 1104 14-15 0.3867679153091004 1104 16-17 0.3450990323203129 1104 18-19 0.3708324111610537 1104 20-21 0.39247423372470536 1104 22-23 0.4225299246970664 1104 24-25 0.38226355010443314 1104 26-27 0.4273001550719471 1104 28-29 0.38717499109605313 1104 30-31 0.3697482620994421 1104 32-33 0.3544430446734097 1104 34-35 0.37729422038569993 1104 36-37 0.3247227413540017 1104 38-39 0.32232444020731066 1104 40-41 0.3981193379688577 1104 42-43 0.264044817035348 1104 44-45 0.2943178143089469 1104 46-47 0.2747913538408753 1104 48-49 0.2934886835115762 1104 50-51 0.31971847784916463 1104 52-53 0.37026324300678226 1104 54-55 0.4109672326853371 1104 56-57 0.3392614610441953 1104 58-59 0.3613725993513057 1104 60-61 0.4067116648970739 1104 62-63 0.38647232626207284 1104 64-65 0.3598437299081283 1104 66-67 0.36618746676776226 1104 68-69 0.34915864502557525 1104 70-71 0.2768849340705444 1104 72-73 0.3045478680177176 1104 74-75 0.3356274072169434 1104 76-77 0.22644769864304592 1104 78-79 0.3397788050181738 1104 80-81 0.3098388696343797 1104 82-83 0.30656056085098626 1104 84-85 0.27051689979269966 1104 86-87 0.3254109300220982 1104 88-89 0.24088665763312989 1104 90-91 0.2566111735772303 1104 92-93 0.26899445829467084 1104 94-95 0.2903946123821015 1104 96-97 0.255193284884367 1104 98-99 0.14565658118124603 1104 100-101 -0.030634973509584995 1105 1 0.14429903796847654 1105 2 0.09713386961847448 1105 3 0.1269264888507351 1105 4 0.1544802063023667 1105 5 0.1577386535268488 1105 6 0.18395426501049172 1105 7 0.17221214329059364 1105 8 0.1785414062084456 1105 9 0.17477393790410645 1105 10-11 0.22215436205618033 1105 12-13 0.17406663029608893 1105 14-15 0.23987700471199247 1105 16-17 0.24288056504364874 1105 18-19 0.19418339923458916 1105 20-21 0.20070560334891496 1105 22-23 0.2671243696006016 1105 24-25 0.2530707151305407 1105 26-27 0.2919167627201986 1105 28-29 0.22368997760025167 1105 30-31 0.2366302998238936 1105 32-33 0.18763708604698337 1105 34-35 0.1802354065917413 1105 36-37 0.1822036412550645 1105 38-39 0.15794400308064382 1105 40-41 0.2516146693432617 1105 42-43 0.20362672052849717 1105 44-45 0.16861962410819586 1105 46-47 0.12455927748137441 1105 48-49 0.2332777513491493 1105 50-51 0.2077881032459743 1105 52-53 0.22424303604287843 1105 54-55 0.2762720329057302 1105 56-57 0.28341650701117516 1105 58-59 0.3257685289480676 1105 60-61 0.26628871419839584 1105 62-63 0.21099810642041206 1105 64-65 0.2631938460771721 1105 66-67 0.22900419955576723 1105 68-69 0.25059802813458276 1105 70-71 0.15595516335572057 1105 72-73 0.20411411819945258 1105 74-75 0.25719001517462203 1105 76-77 0.24620322745215617 1105 78-79 0.31708405165982967 1105 80-81 0.2776037354594685 1105 82-83 0.2604645835564945 1105 84-85 0.2671398366088198 1105 86-87 0.29746360984308495 1105 88-89 0.26825016723533324 1105 90-91 0.2349243378326804 1105 92-93 0.2260111736163708 1105 94-95 0.13487246336726955 1105 96-97 0.16698122083698763 1105 98-99 0.12832658948131836 1105 100-101 0.06841450647580416 1106 1 0.09064356691975917 1106 2 0.04045270459291572 1106 3 0.0498189102202673 1106 4 0.08403677252371011 1106 5 0.11042238827305795 1106 6 0.060285695494116 1106 7 0.08055951266442918 1106 8 0.07755263904733312 1106 9 0.09548277652054793 1106 10-11 0.06256570440560694 1106 12-13 0.11524624665619143 1106 14-15 0.07697752238453148 1106 16-17 0.09626176645054585 1106 18-19 0.0802715817698143 1106 20-21 0.08870664173172571 1106 22-23 0.08878031350068483 1106 24-25 0.06286323646076397 1106 26-27 0.07354858496678673 1106 28-29 0.07813725817806727 1106 30-31 0.03982222218187559 1106 32-33 -0.014400834057418876 1106 34-35 0.04625840761723765 1106 36-37 -0.006908412379765139 1106 38-39 0.01693697279430495 1106 40-41 0.08760507775917148 1106 42-43 -0.0033088616711367536 1106 44-45 -0.019905611030566206 1106 46-47 -0.002276031995101846 1106 48-49 0.013107876971595545 1106 50-51 -0.010967362134927328 1106 52-53 0.05312252291901842 1106 54-55 0.06986274552481575 1106 56-57 0.11117930316083857 1106 58-59 0.12295102380108602 1106 60-61 0.0913596268849659 1106 62-63 0.08444197940320919 1106 64-65 0.05613865494675707 1106 66-67 0.029980714207795245 1106 68-69 0.06298210089368439 1106 70-71 0.08480008793910798 1106 72-73 0.068121205091348 1106 74-75 0.045930749804309556 1106 76-77 0.052164722277133535 1106 78-79 0.05863116023279602 1106 80-81 0.08218725464046983 1106 82-83 -0.01294922001589427 1106 84-85 0.09416044331066686 1106 86-87 0.10633261664678884 1106 88-89 0.01569755183529864 1106 90-91 0.07293536030887893 1106 92-93 0.022354264546933678 1106 94-95 0.03129168109472147 1106 96-97 -0.05725551000070084 1106 98-99 -0.07814546902612918 1106 100-101 0.005050118682152771 1107 1 -0.02158550883367738 1107 2 -0.0339590851079663 1107 3 -0.017082986214099094 1107 4 0.04016763972538229 1107 5 -0.017009906780501183 1107 6 0.03844339606976632 1107 7 0.0345066995046821 1107 8 0.03868210219073376 1107 9 0.10412976200401403 1107 10-11 0.10841222010640905 1107 12-13 0.09176428388496305 1107 14-15 0.04734852119226929 1107 16-17 0.006387189021772599 1107 18-19 -0.031148963322415568 1107 20-21 -0.041554530684607016 1107 22-23 0.04920304222781624 1107 24-25 -0.014119241178974562 1107 26-27 0.06036844764508231 1107 28-29 -0.029445509127221214 1107 30-31 -0.05575085625254417 1107 32-33 -0.08239826316995646 1107 34-35 -0.06953535265002131 1107 36-37 -0.10610718740813496 1107 38-39 -0.04757944652453716 1107 40-41 0.00664185802423134 1107 42-43 -0.039594162856083415 1107 44-45 -0.019592998942307815 1107 46-47 -0.05680338696593168 1107 48-49 -0.04378043864905834 1107 50-51 -0.08539238370597957 1107 52-53 -0.03504151390145438 1107 54-55 -0.01692364372951971 1107 56-57 0.005453596884571255 1107 58-59 -0.0618531474501367 1107 60-61 -0.08850456366463533 1107 62-63 -0.035413255299410196 1107 64-65 -0.09342733112443824 1107 66-67 -0.05895025079908578 1107 68-69 -0.04197319180185133 1107 70-71 -0.10559177150926047 1107 72-73 -0.08387869497380862 1107 74-75 -0.23643315443782242 1107 76-77 -0.16414266539699085 1107 78-79 -0.042852305925382694 1107 80-81 -0.04761278242796507 1107 82-83 -0.17241430180001416 1107 84-85 -0.10739854881674304 1107 86-87 -0.17036245790306737 1107 88-89 -0.18997446067866264 1107 90-91 -0.19715776114319183 1107 92-93 -0.23854455591076373 1107 94-95 -0.1794993578372761 1107 96-97 -0.22383073810510723 1107 98-99 -0.3036681508812755 1107 100-101 -0.3325641982104628 1108 1 -0.0986404281265969 1108 2 -0.13741095171019424 1108 3 -0.1927828951841235 1108 4 -0.11334136023448593 1108 5 -0.08826272404075297 1108 6 -0.07066211696447056 1108 7 -0.07353757420177942 1108 8 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2316 74-75 -0.20885173705804405 2316 76-77 -0.29964140210403656 2316 78-79 -0.1964333105011029 2316 80-81 -0.20967521867741468 2316 82-83 -0.08387462040376548 2316 84-85 -0.19202225716817978 2316 86-87 -0.2204015103675374 2316 88-89 -0.2122325406773271 2316 90-91 -0.23285395955004873 2316 92-93 -0.3000015843005954 2316 94-95 -0.31508597993983756 2316 96-97 -0.22625482174205302 2316 98-99 -0.19273740115478688 2316 100-101 -0.31628108578838976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 132203.0 3 29678.0 4 27672.0 5 39965.0 6 91117.0 7 205021.0 8 385638.0 9 632441.0 10 826634.0 11 736477.0 12 497462.0 13 329073.0 14 249891.0 15 211966.0 16 193021.0 17 186449.0 18 186239.0 19 192889.0 20 206649.0 21 225572.0 22 249917.0 23 280907.0 24 314488.0 25 356138.0 26 412779.0 27 484473.0 28 577466.0 29 694649.0 30 846407.0 31 1045344.0 32 1315403.0 33 1695165.0 34 2256824.0 35 3140964.0 36 4677833.0 37 8374570.0 38 1.1690219E7 39 3186036.0 40 1316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.990896289723953 16.94010110305206 8.709591521339059 43.35941108588492 2 14.941932054224075 16.456512332378693 55.79812256420161 12.80343304919562 3 17.838028646760044 22.494729901616424 37.41622240627944 22.25101904534409 4 20.2580993744406 26.932341911419766 34.640920324306094 18.16863838983354 5 19.617520191951602 30.296224543658894 36.77832563524865 13.307929629140853 6 14.443420014908629 35.66988471776507 35.854576646016284 14.032118621310014 7 12.003455045943392 19.35067569645592 54.176153887909095 14.46971536969159 8 12.902718558550768 21.820420495452613 47.853551897976885 17.423309048019732 9 13.924681090356433 22.897843270454725 45.2600724077237 17.917403231465137 10-11 16.785589542550365 32.031721818368254 36.17217127246472 15.010517366616657 12-13 16.327439913808323 25.985245954689606 41.74619629256181 15.941117838940249 14-15 16.986224264735878 28.317575270538224 39.7895064430413 14.906694021684594 16-17 18.730870361302024 28.18966297885806 37.751477787669174 15.327988872170744 18-19 18.602515273300313 28.773423864070807 36.96027945879918 15.663781403829704 20-21 18.82523021965556 29.172200009729675 35.65752868604254 16.345041084572223 22-23 18.099189087390112 29.394592942486025 35.99225761233381 16.513960357790058 24-25 17.90490004866086 29.885589701874636 34.938620332211066 17.270889917253438 26-27 17.429326000952493 30.56487783049456 34.88887437819209 17.116921790360855 28-29 18.08207161177054 30.30763198625501 34.0263572240456 17.583939177928848 30-31 18.090782528395163 30.699645636039065 33.39359438971995 17.81597744584582 32-33 17.862632783678862 30.210813042743933 33.52386614460189 18.402688028975316 34-35 18.44714884771991 31.318898334485155 32.44562106437904 17.788331753415896 36-37 18.857905247220614 31.25597477251281 32.6351603239057 17.25095965636087 38-39 18.994715996732033 30.70618676959061 32.8191999774848 17.47989725619255 40-41 18.734886375185216 30.745218096780796 33.06439067228665 17.455504855747343 42-43 18.295448699272242 30.988385033790642 33.234425899190164 17.48174036774695 44-45 18.41769936661804 30.178445088129212 33.30491397150822 18.09894157374452 46-47 17.937991532512203 31.04529064500748 32.89587557020707 18.12084225227325 48-49 17.405856006934677 31.071854532892285 32.98306546772234 18.539223992450697 50-51 17.403675064255058 30.93391855329684 33.65292530354896 18.00948107889914 52-53 18.01173392285524 30.837277144286535 32.726181141586345 18.424807791271878 54-55 18.45588822585426 31.580767198416794 31.802881721769694 18.160462853959256 56-57 19.191534990306465 31.441587350286593 31.79810466409266 17.568772995314287 58-59 18.440559115448245 31.742687838102214 32.3349747083459 17.48177833810364 60-61 18.892825782169773 32.27610469498289 30.747390275241866 18.083679247605474 62-63 18.38902639936098 32.75161666041452 31.22038563796723 17.63897130225727 64-65 18.644165543185604 31.201722764101365 31.987556698575087 18.166554994137947 66-67 18.35174597868616 32.71808779463145 30.745705114382503 18.184461112299886 68-69 18.35773021036434 31.69004019374234 31.71753985328223 18.234689742611092 70-71 18.04584901142353 31.555204023832268 31.543072300788054 18.855874663956147 72-73 18.16054325604462 31.999283655666204 31.19977026116632 18.640402827122852 74-75 18.509072635603907 31.34096069055914 31.286196787410454 18.863769886426503 76-77 18.387898383607755 31.917620817078024 30.973071016190946 18.721409783123274 78-79 18.4393747566965 32.56303142774194 30.95203841109569 18.045555404465873 80-81 18.60502208416117 32.77405202852872 30.867846138812443 17.75307974849766 82-83 18.596901438789985 32.15558836647361 31.143047822120977 18.104462372615426 84-85 18.525611917677917 32.294548946786115 30.958088656015686 18.221750479520278 86-87 18.235983375504276 32.58698784358156 31.207582677682744 17.96944610323142 88-89 18.49316645071182 32.16681011963721 31.06753024302238 18.272493186628587 90-91 17.849624366464376 33.15741824963711 30.654091292719492 18.33886609117902 92-93 18.646587912723447 32.01777679267721 30.984273712859462 18.35136158173988 94-95 18.666295405482757 32.98514750995076 30.000477523618496 18.34807956094799 96-97 18.630483606657137 32.96259864933512 30.320973420820813 18.08594432318693 98-99 19.145547979118884 32.511565289233694 29.96809332623618 18.374793405411243 100-101 19.349412684710916 33.71043150112497 28.828331184976342 18.111824629187772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14749.0 1 21383.5 2 26320.0 3 29625.0 4 41155.5 5 55532.5 6 68753.0 7 81543.0 8 96973.5 9 112152.5 10 127107.5 11 141776.5 12 155625.0 13 168071.5 14 182113.5 15 196188.0 16 207927.5 17 222916.0 18 242969.0 19 266195.0 20 297892.5 21 341829.5 22 398486.5 23 464838.5 24 551627.5 25 680236.0 26 805281.0 27 944725.5 28 1086231.5 29 1226002.5 30 1410672.5 31 1604626.5 32 1763862.0 33 1977931.5 34 2128375.0 35 2343856.5 36 2384592.0 37 2288282.0 38 2392541.0 39 2271258.0 40 2152314.0 41 2089757.0 42 1756441.5 43 1433532.0 44 1272049.5 45 1149538.5 46 1049718.0 47 944752.0 48 873051.0 49 800893.5 50 682655.5 51 558848.5 52 475769.0 53 417696.0 54 355776.5 55 286658.0 56 234948.5 57 197077.5 58 161194.0 59 128898.5 60 97049.0 61 68912.0 62 51152.5 63 38766.5 64 27282.0 65 18282.0 66 12069.5 67 7982.0 68 5218.0 69 3336.0 70 2535.5 71 2516.0 72 2534.5 73 2921.5 74 2691.0 75 1752.5 76 1420.5 77 1274.5 78 1011.5 79 639.5 80 406.5 81 260.5 82 160.0 83 107.0 84 61.5 85 38.5 86 35.5 87 22.5 88 12.0 89 11.0 90 13.5 91 15.0 92 10.5 93 8.0 94 6.5 95 3.0 96 5.5 97 6.0 98 3.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04873804635200555 2 0.0014431954763768928 3 0.004984428429425039 4 0.028603244265284763 5 0.0681374757070042 6 0.013192205345735914 7 0.015394085081353521 8 0.0 9 0.0 10-11 1.0596148872077038E-6 12-13 6.781535278129304E-5 14-15 0.0 16-17 7.205381233012386E-5 18-19 7.417304210453928E-5 20-21 0.003010365894557087 22-23 0.0015597531139697402 24-25 0.009050170751641 26-27 0.007146042799328755 28-29 1.1655763759284743E-5 30-31 0.0024296969363672653 32-33 0.0018151203017867969 34-35 0.0020026721368225607 36-37 2.5430757292984895E-5 38-39 0.002215654729151309 40-41 0.20029476366932342 42-43 0.004084815390185698 44-45 0.0017261126512613495 46-47 0.007750023285037146 48-49 0.008010688547290243 50-51 0.018159679936965628 52-53 0.0043507787268748325 54-55 0.010571777729671262 56-57 0.008419699893752415 58-59 0.012036165503792308 60-61 0.012862665115814317 62-63 0.02363788890382946 64-65 0.010815489153729034 66-67 0.035008616258455326 68-69 0.025060951697349405 70-71 0.01031852977162862 72-73 0.006235833611217338 74-75 0.022902516172107312 76-77 0.010722243043654756 78-79 0.03920787005645946 80-81 0.0882192970493646 82-83 0.054009630415863874 84-85 0.08331539935136735 86-87 0.03484331633605093 88-89 0.0139191011583604 90-91 0.020043675206420926 92-93 0.02750442362725037 94-95 0.05718529623282536 96-97 0.05234709465783499 98-99 0.05969658351550763 100-101 0.0512207240327332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.7186955E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.878099074001348 #Duplication Level Percentage of deduplicated Percentage of total 1 79.30393525497689 21.31539028741457 2 8.790386513543428 4.72537759219571 3 3.4445630331389774 2.777499194140561 4 1.801812903637533 1.9371722294713463 5 1.155240617913759 1.5525335891298278 6 0.8194842039273305 1.3215706573642745 7 0.5923348182770553 1.114458375144189 8 0.44096191323843975 0.9481774393507176 9 0.35968228726208884 0.8700818536974454 >10 2.648373498159389 14.186040150156712 >50 0.3375749969124804 6.329273073136892 >100 0.25670707573936713 13.563523586960827 >500 0.027190776049646342 5.036691370833316 >1k 0.018174242886853806 9.55426499314485 >5k 0.002086426451132517 3.854296218826235 >10k+ 0.001491437885624912 10.913649389032622 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT 215539 0.4567766663477225 No Hit GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT 152617 0.32343049048195627 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 131428 0.2785261307918682 No Hit GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT 106319 0.22531439038607176 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 104171 0.22076228483062743 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 101279 0.21463347232301808 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 90911 0.19266129802187915 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 85849 0.18193375690378835 No Hit ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 82906 0.1756968636776838 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 76832 0.16282466202788462 No Hit ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA 75770 0.16057404000745545 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 75301 0.1595801212432546 No Hit GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG 67931 0.14396139780581307 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66949 0.14188031416733712 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 64389 0.1364550859448337 No Hit CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG 62696 0.1328672299367484 No Hit ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA 60204 0.1275861093389052 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 58593 0.12417203017232199 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 56029 0.11873832503072088 No Hit GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 54035 0.11451258086053658 No Hit ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT 50071 0.10611195403475387 No Hit GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT 48631 0.1030602631595957 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 47860 0.10142633700352141 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 47693 0.10107242563119404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.07787957497999182 0.0 0.0 8.476919097661632E-6 0.0 2 0.07813600178269609 2.119229774415408E-6 0.0 1.2715378646492447E-5 0.0 3 0.0781529556208914 2.119229774415408E-6 0.0 1.4834608420907855E-5 0.0 4 0.07877177071502071 2.119229774415408E-6 0.0 1.4834608420907855E-5 0.0 5 0.07889892450148564 2.119229774415408E-6 0.0 1.6953838195323264E-5 0.0 6 0.07905150904524354 2.119229774415408E-6 0.0 2.7549987067400302E-5 0.0 7 0.07961946262478688 2.119229774415408E-6 0.0 4.874228481155438E-5 0.0 8 0.08781452416245125 4.238459548830816E-6 0.0 5.29807443603852E-5 0.0 9 0.08791412796184878 4.238459548830816E-6 0.0 7.205381233012387E-5 0.0 10-11 0.08915387737988179 5.29807443603852E-6 0.0 7.417304210453928E-5 0.0 12-13 0.09017640574603722 6.357689323246224E-6 0.0 8.159034631499321E-5 0.0 14-15 0.09103787264933709 6.357689323246224E-6 0.0 1.1231917804401662E-4 0.0 16-17 0.09170543002827794 6.357689323246224E-6 0.0 1.32451860900963E-4 0.0 18-19 0.09241855084736872 6.357689323246224E-6 0.0 1.6106146285557098E-4 0.0 20-21 0.09339975423292307 6.357689323246224E-6 0.0 1.875518350357636E-4 0.0 22-23 0.09441592490975526 6.357689323246224E-6 0.0 1.9814798390784064E-4 0.0 24-25 0.0953854725315503 6.357689323246224E-6 0.0 2.3841334962173337E-4 0.0 26-27 0.09623952213063972 7.417304210453928E-6 0.0 3.4013637879367297E-4 0.0 28-29 0.0971041678786012 8.476919097661632E-6 0.0 3.984151975900967E-4 0.0 30-31 0.09791583288220229 1.2715378646492447E-5 0.0 4.2596518465749697E-4 0.0 32-33 0.09850497875948977 1.2715378646492447E-5 0.0 4.2702479954470466E-4 0.0 34-35 0.09908140925813078 1.2715378646492447E-5 0.0 4.2914402931912006E-4 0.0 36-37 0.09940988987316515 1.2715378646492447E-5 0.0 4.588132461609358E-4 0.0 38-39 0.09968750897361357 1.3774993533700151E-5 0.0 4.672901652585974E-4 0.0 40-41 0.09997996268248291 1.4834608420907855E-5 0.0 4.8000554390508984E-4 0.0 42-43 0.10032539713571262 1.4834608420907855E-5 0.0 5.075555309724902E-4 0.0 44-45 0.1008181180582642 1.4834608420907855E-5 0.0 5.499401264607983E-4 0.0 46-47 0.10167746573178965 1.4834608420907855E-5 0.0 6.05040100595599E-4 0.0 48-49 0.10235455964471536 1.4834608420907855E-5 0.0 6.198747090165068E-4 0.0 50-51 0.10301575933433296 1.4834608420907855E-5 0.0 6.304708578885839E-4 0.0 52-53 0.10379669550620504 1.4834608420907855E-5 0.0 6.357689323246223E-4 0.0 54-55 0.10529393134182954 1.4834608420907855E-5 1.059614887207704E-6 6.410670067606608E-4 0.0 56-57 0.1061850674619712 1.589422330811556E-5 2.119229774415408E-6 6.527227705199456E-4 0.0 58-59 0.10751382453052968 1.6953838195323264E-5 2.119229774415408E-6 6.717958384896844E-4 0.0 60-61 0.11055385964192857 1.6953838195323264E-5 2.119229774415408E-6 6.802727575873459E-4 0.0 62-63 0.11367866394430409 1.6953838195323264E-5 2.119229774415408E-6 7.162996637524077E-4 0.0 64-65 0.11535073623631786 1.6953838195323264E-5 2.119229774415408E-6 7.544457996918852E-4 0.0 66-67 0.11992297447461911 1.6953838195323264E-5 2.119229774415408E-6 7.841150165337009E-4 0.0 68-69 0.12499429132479517 1.6953838195323264E-5 2.119229774415408E-6 7.841150165337009E-4 0.0 70-71 0.12621390805997124 1.8013453082530968E-5 2.119229774415408E-6 7.872938611953239E-4 0.0 72-73 0.13102244041811978 1.9073067969738672E-5 2.119229774415408E-6 7.947111654057779E-4 0.0 74-75 0.13369902762320646 1.9073067969738672E-5 2.119229774415408E-6 8.349765311196707E-4 0.0 76-77 0.14037142256795337 1.9073067969738672E-5 2.119229774415408E-6 9.706072366822568E-4 0.0 78-79 0.1504314063071033 1.9073067969738672E-5 2.119229774415408E-6 0.0010204091363810188 0.0 80-81 0.15665664376944857 1.9073067969738672E-5 2.119229774415408E-6 0.0010861052593878965 0.0 82-83 0.1669613985475435 1.9073067969738672E-5 2.119229774415408E-6 0.0011253110102145817 0.0 84-85 0.18704745834945272 1.9073067969738672E-5 2.119229774415408E-6 0.0011316686995378276 0.0 86-87 0.24070847546742527 1.9073067969738672E-5 2.119229774415408E-6 0.001145443693071528 0.0 88-89 0.33616176335175685 1.9073067969738672E-5 2.119229774415408E-6 0.0011549802270563972 2.119229774415408E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 44955 0.0 63.711983 1 GCGTGTT 59900 0.0 47.704025 2 CCCGAAA 12955 0.0 45.720486 1 GTTTTTT 715125 0.0 45.165035 1 CGTGTTT 79345 0.0 36.12698 3 CCGAAAC 17085 0.0 34.52873 2 CGGCCTT 5185 0.0 33.98654 1 CGCCGTA 6665 0.0 33.694756 46-47 GTCGCCG 7120 0.0 33.640038 44-45 CCCAGAT 31730 0.0 33.561966 1 TCGTCTA 15875 0.0 31.13238 44-45 CAAAACG 49710 0.0 31.033348 9 GATATAT 29715 0.0 31.009773 5 CGAGCTA 16185 0.0 30.79408 12-13 CGAAACC 19215 0.0 30.577593 3 CTCGTAT 4810 0.0 29.723724 1 ACTCGTC 16565 0.0 29.704916 42-43 CTAACGA 15745 0.0 29.089876 94-95 TCAACGG 15610 0.0 29.056608 16-17 CGGACCA 15615 0.0 29.021149 20-21 >>END_MODULE