FastQCFastQC Report
Fri 27 May 2016
SRR1233045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47186955
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT2105830.4462737635857198No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT1509380.3198723036907128No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1128470.23914872235345552No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1056990.22400046792593417No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1043770.22119884616415703No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1009830.21400618030979116No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA948680.2010470902392409No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT882640.18705169680900155No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT864510.18320953322798642No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT829270.17574136750294653No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC827650.17539805227949123No Hit
ATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCA794690.16841307094301805No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT765040.16212955466187634No Hit
GGATAATACGACTCACTATAGGATAATACGACTCACTATAGGATAATACG706190.1496578874394417No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC695010.14728858855164526No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT622500.13192205345735913No Hit
ATACGACTCACTATAGGAGATAATACGACTCACTATAGGAGATAATACGA618820.13114217690037427No Hit
CTCACTATAGGATAATACGACTCACTATAGGATAATACGACTCACTATAG617650.13089422701676767No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC570020.12080033560122706No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA544630.11541961120398636No Hit
ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT540510.11454648853692721No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA536970.11379628119678414No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC518890.1099647137646411TruSeq Adapter, Index 11 (100% over 50bp)
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA511620.1084240337186411No Hit
GTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTATCCT496030.10512015450032748No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC472570.10014844144954893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT460050.063.6563841
CCCGAAA119550.047.903891
GCGTGTT616250.047.3739742
GTTTTTT6032950.039.8103871
TATGCCG163750.037.3244846-47
CGTGTTT791500.037.1775363
CCGAAAC162350.035.128022
TGCCGTC174700.034.64652648-49
CGGCCTT52950.034.1689831
CCCAGAT302300.033.8832471
CCGTCTT191650.031.59640750-51
CGAGCTA156350.031.52308812-13
TCGTCTA152750.031.37003544-45
CTCGTAT192350.031.18091242-43
TCACGGC116850.030.80515730-31
CGAAACC184550.030.772813
CGTCTGA204400.030.52311316-17
AGTCACG119850.030.32630228-29
GATATAT305900.030.1912125
ACGGCTA123650.029.7262232-33
Produced by FastQC (version 0.11.5)