FastQCFastQC Report
Fri 27 May 2016
SRR1233044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53018484
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3468610.6542265523850135Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1425030.26877984666630605No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA1023490.19304399575061407No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG972370.18340207539695025Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT868670.1638428590300696No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735330.13869313954733223No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA705320.13303284944926No Hit
CAAGCAGAAGACGGCATACGAGATCTGAAGAGCGTCGTGTAGGGAAAGAG688540.12986791549905502Illumina Single End PCR Primer 1 (96% over 30bp)
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC592950.11183835433695162No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT573250.10812266906764063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC306100.067.9455959
CGCGTGT484900.064.471811
AAGACGG350300.060.497928
GCGTGTT626150.049.8904152
GAAGACG450400.047.178737
ATCGGGA108450.042.2892152
GAGCGGC130900.041.892369
GTTTTTT9367700.040.3884661
TCGGGAG117750.037.629863
CGGCATA271800.035.8477612-13
CGCCGTA836150.035.31802446-47
CAAGCAG592250.035.051431
CCGTATC849150.034.9451848-49
ACGGCAT285800.034.2688910-11
GTCGCCG875600.034.10414544-45
AGAGCGG168050.033.95328
CCGAGAT121650.033.5590171
AGAGCGT1385300.033.401779
GACGGCA299950.032.9932310-11
CCCGAAA100700.032.989511
Produced by FastQC (version 0.11.5)