##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233044_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53018484 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.397262396261652 33.0 31.0 34.0 26.0 34.0 2 30.556427829962093 33.0 31.0 34.0 26.0 34.0 3 30.49733651380903 33.0 31.0 34.0 25.0 34.0 4 33.72377827702505 37.0 35.0 37.0 28.0 37.0 5 33.667376475721184 37.0 35.0 37.0 28.0 37.0 6 33.66332747273574 37.0 35.0 37.0 28.0 37.0 7 33.833168485164535 37.0 35.0 37.0 28.0 37.0 8 33.82488205434165 37.0 35.0 37.0 28.0 37.0 9 35.238896627070666 39.0 37.0 39.0 28.0 39.0 10-11 35.08411362723989 39.0 35.0 39.0 27.0 39.0 12-13 34.984413492471795 39.0 35.0 39.0 27.0 39.0 14-15 36.04559271253399 40.0 36.0 41.0 26.5 41.0 16-17 35.75885628868603 40.0 36.0 41.0 25.5 41.0 18-19 35.2907343031536 39.5 35.5 41.0 24.0 41.0 20-21 34.862668470490405 39.0 35.0 41.0 18.5 41.0 22-23 34.28335482960999 39.0 34.0 41.0 14.5 41.0 24-25 33.87825854281311 39.0 34.0 41.0 10.5 41.0 26-27 33.45371139808524 39.0 33.5 41.0 8.0 41.0 28-29 32.980587609785296 39.0 33.0 41.0 2.0 41.0 30-31 32.69498965681478 39.0 32.0 41.0 2.0 41.0 32-33 32.55218458339925 39.0 32.0 41.0 2.0 41.0 34-35 32.39458029392165 39.0 32.0 41.0 2.0 41.0 36-37 32.224055171023 39.0 31.0 41.0 2.0 41.0 38-39 32.09317605158231 39.0 31.0 41.0 2.0 41.0 40-41 31.939731905574668 39.0 31.0 41.0 2.0 41.0 42-43 31.80785485114965 39.0 30.5 41.0 2.0 41.0 44-45 31.68314262814455 38.5 30.0 41.0 2.0 41.0 46-47 31.532171308406326 39.0 30.0 41.0 2.0 41.0 48-49 31.48022533047154 39.0 30.0 41.0 2.0 41.0 50-51 30.93041222189605 38.5 29.5 40.5 2.0 41.0 52-53 30.98404275384411 38.0 30.0 40.0 2.0 41.0 54-55 31.182507161087443 38.0 30.0 41.0 2.0 41.0 56-57 31.012985659869113 38.0 29.5 41.0 2.0 41.0 58-59 30.845022652854425 38.0 29.0 41.0 2.0 41.0 60-61 30.67876337241178 38.0 28.5 41.0 2.0 41.0 62-63 30.21272083147455 38.0 27.5 40.0 2.0 41.0 64-65 30.00660615833527 37.0 27.5 40.0 2.0 41.0 66-67 29.662457662878477 37.0 26.0 40.0 2.0 41.0 68-69 29.370255569736774 36.5 26.0 40.0 2.0 41.0 70-71 28.988291743686975 36.0 25.5 39.0 2.0 41.0 72-73 28.608213637342025 35.5 24.5 39.0 2.0 41.0 74-75 28.147151020010305 35.0 22.5 39.0 2.0 40.5 76-77 27.742205473095005 35.0 22.0 38.0 2.0 40.0 78-79 27.320403163545755 35.0 20.5 37.0 2.0 39.0 80-81 26.773715210340605 34.0 18.0 36.5 2.0 39.0 82-83 26.38935421088238 34.0 17.5 36.0 2.0 39.0 84-85 25.78304772916555 34.0 9.0 36.0 2.0 37.0 86-87 25.34613346356716 34.0 4.5 35.0 2.0 37.0 88-89 24.979371175531917 33.5 2.0 35.0 2.0 36.5 90-91 24.56776469693098 33.0 2.0 35.0 2.0 36.0 92-93 24.013112945666272 32.5 2.0 35.0 2.0 36.0 94-95 23.384030510944072 32.0 2.0 35.0 2.0 35.0 96-97 22.713474728926613 31.0 2.0 35.0 2.0 35.0 98-99 21.93664021966377 31.0 2.0 34.0 2.0 35.0 100-101 20.438385158278006 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9374917468445467 1101 2 1.0012164877702716 1101 3 1.017226055202542 1101 4 1.0485444100039132 1101 5 1.0782115803495316 1101 6 1.0517735495937615 1101 7 0.904040267180406 1101 8 0.8877267086237666 1101 9 1.1072453843823666 1101 10-11 1.1207663586303056 1101 12-13 1.1284564718283718 1101 14-15 1.360519521111641 1101 16-17 1.3471178626127056 1101 18-19 1.292456109691848 1101 20-21 1.3671971539649164 1101 22-23 1.4984348434078854 1101 24-25 1.4186957445186792 1101 26-27 1.3233795845181433 1101 28-29 1.2775478218874028 1101 30-31 1.225103121464663 1101 32-33 1.2766349043437089 1101 34-35 1.263459686644154 1101 36-37 1.3087013125538434 1101 38-39 1.2785198064157157 1101 40-41 1.2664354594877665 1101 42-43 1.2489733514810908 1101 44-45 1.2656325215484188 1101 46-47 1.435978320690399 1101 48-49 1.4171668085170026 1101 50-51 1.2145104003711253 1101 52-53 1.2415296230159925 1101 54-55 1.3221347925917257 1101 56-57 1.4034647005928989 1101 58-59 1.456943334463773 1101 60-61 1.4084061665060297 1101 62-63 1.5504750734940274 1101 64-65 1.3644273897826764 1101 66-67 1.3503155577562929 1101 68-69 1.320817047793387 1101 70-71 1.3328289713223676 1101 72-73 1.2470414099208789 1101 74-75 1.329434777460147 1101 76-77 1.3129084436906346 1101 78-79 1.264641903559994 1101 80-81 1.3736591865016656 1101 82-83 1.2870338700260398 1101 84-85 1.4065874283563744 1101 86-87 1.2945202955517239 1101 88-89 1.1309261603335834 1101 90-91 1.0469917909260218 1101 92-93 1.0002858911271169 1101 94-95 0.9839251047140216 1101 96-97 0.8842958273312824 1101 98-99 0.7771385452405788 1101 100-101 0.6242704465523659 1102 1 0.7468109539456727 1102 2 0.8024124443795451 1102 3 0.7344204874188094 1102 4 0.7860266211140825 1102 5 0.8143693771929961 1102 6 0.8450223745780647 1102 7 0.8100264229093312 1102 8 0.8331439634023141 1102 9 0.9781218630406983 1102 10-11 0.9839542205747165 1102 12-13 1.0086844662721006 1102 14-15 1.181168502249534 1102 16-17 1.2199141827914346 1102 18-19 1.1914104392014266 1102 20-21 1.2062418699081476 1102 22-23 1.3964228926059974 1102 24-25 1.3045614686373384 1102 26-27 1.1963162211695106 1102 28-29 1.0718972215466707 1102 30-31 1.0980941694309507 1102 32-33 1.1315841493637748 1102 34-35 1.2149411135051054 1102 36-37 1.2640616274635548 1102 38-39 1.1956168332010506 1102 40-41 1.203981240870874 1102 42-43 1.1860884608351938 1102 44-45 1.2159995223097368 1102 46-47 1.359891329238426 1102 48-49 1.2716430486959283 1102 50-51 1.1022038613621987 1102 52-53 1.1553159830819908 1102 54-55 1.2053301363038145 1102 56-57 1.2561028022240848 1102 58-59 1.2841257932683519 1102 60-61 1.2250196974751368 1102 62-63 1.4298638879379055 1102 64-65 1.2102564565967704 1102 66-67 1.1606203601011238 1102 68-69 1.1867440619041751 1102 70-71 1.1441492510712052 1102 72-73 1.1206170601343253 1102 74-75 1.2543755458432138 1102 76-77 1.2409933626971643 1102 78-79 1.2034325894219826 1102 80-81 1.3427591236280527 1102 82-83 1.33893711649959 1102 84-85 1.5366198531080997 1102 86-87 1.4608571543106166 1102 88-89 1.3463188007683193 1102 90-91 1.3096033334990729 1102 92-93 1.282759628321692 1102 94-95 1.410537833502456 1102 96-97 1.4306251671387074 1102 98-99 1.508345204577708 1102 100-101 1.5398073488178987 1103 1 0.5711300818849807 1103 2 0.6554017217343286 1103 3 0.6191880534138292 1103 4 0.6815808228058344 1103 5 0.6620771314917278 1103 6 0.7025605083844866 1103 7 0.6278305624907006 1103 8 0.5938426170293312 1103 9 0.7092741159148943 1103 10-11 0.6933237856878804 1103 12-13 0.7974949541168996 1103 14-15 0.8574027730446758 1103 16-17 0.8352015435481945 1103 18-19 0.8741654780147741 1103 20-21 0.8790000964246119 1103 22-23 1.0357699399840428 1103 24-25 0.9408861203215722 1103 26-27 0.8623679887120161 1103 28-29 0.922360413422119 1103 30-31 0.8793496487145376 1103 32-33 0.8723142183476256 1103 34-35 0.8695638629895726 1103 36-37 0.936141335881409 1103 38-39 0.8891139386158571 1103 40-41 0.8768919250998977 1103 42-43 0.843898744557734 1103 44-45 0.8392302021592783 1103 46-47 1.0292998715976331 1103 48-49 0.9997565288874526 1103 50-51 0.9001768046409993 1103 52-53 0.892307577088971 1103 54-55 0.9461864070697743 1103 56-57 1.0673627180950191 1103 58-59 1.0741668833287576 1103 60-61 1.0524843381447688 1103 62-63 1.232062978381009 1103 64-65 1.0716167106201375 1103 66-67 1.050497832775914 1103 68-69 0.9942124118543347 1103 70-71 1.0155149735099336 1103 72-73 1.011595256986027 1103 74-75 1.1325529580852773 1103 76-77 1.1414462638071932 1103 78-79 1.1053214561636047 1103 80-81 1.2353033047912199 1103 82-83 1.199044248885798 1103 84-85 1.349897698842831 1103 86-87 1.2540496170718178 1103 88-89 1.213181391814203 1103 90-91 1.1951647977911968 1103 92-93 1.2085932189261293 1103 94-95 1.3138713639190733 1103 96-97 1.3976404432827962 1103 98-99 1.50181872769177 1103 100-101 1.5311129517115845 1104 1 0.37336003502718285 1104 2 0.4944011680656608 1104 3 0.5057523924295069 1104 4 0.551905836449869 1104 5 0.5229261582108506 1104 6 0.5442681762952404 1104 7 0.4824854085950179 1104 8 0.48579036805855225 1104 9 0.6136221216938509 1104 10-11 0.5660306568086071 1104 12-13 0.5951059746267973 1104 14-15 0.6612852828803 1104 16-17 0.6620702513000367 1104 18-19 0.6084747488337356 1104 20-21 0.652681731202712 1104 22-23 0.791594083346908 1104 24-25 0.7133393527520084 1104 26-27 0.5635727855173016 1104 28-29 0.632378705307957 1104 30-31 0.624930829725912 1104 32-33 0.6266901264006037 1104 34-35 0.606450183746631 1104 36-37 0.6337909536879778 1104 38-39 0.6238362797232426 1104 40-41 0.5848451989861019 1104 42-43 0.6133538032953112 1104 44-45 0.6482825339613214 1104 46-47 0.7671276565769851 1104 48-49 0.7462438960438433 1104 50-51 0.5982359557551398 1104 52-53 0.5902613373390899 1104 54-55 0.6657207088029509 1104 56-57 0.7849278134155497 1104 58-59 0.7847271137623508 1104 60-61 0.710221951983911 1104 62-63 0.9030095566675271 1104 64-65 0.7117855671238829 1104 66-67 0.7245773509343465 1104 68-69 0.6845928672554393 1104 70-71 0.7183828099009979 1104 72-73 0.722893048237534 1104 74-75 0.8323722757405534 1104 76-77 0.843313848542703 1104 78-79 0.7895281813514998 1104 80-81 0.8828664753211903 1104 82-83 0.9073179057327252 1104 84-85 1.0832013481985392 1104 86-87 1.0065064852042234 1104 88-89 0.8623656941581075 1104 90-91 0.8461755178253583 1104 92-93 0.8359182542394947 1104 94-95 0.930585116719481 1104 96-97 0.9676495038118595 1104 98-99 1.050622699109617 1104 100-101 1.0310806778681396 1105 1 0.21155950887620278 1105 2 0.34344676219664905 1105 3 0.29030902028453553 1105 4 0.33097457173212774 1105 5 0.2985027344318212 1105 6 0.31081002003182334 1105 7 0.27340904448612235 1105 8 0.27424723783546057 1105 9 0.35983237971051096 1105 10-11 0.34503187019490866 1105 12-13 0.35427904565623436 1105 14-15 0.33626348818470575 1105 16-17 0.2909304637206276 1105 18-19 0.3397796850062349 1105 20-21 0.3339457471794347 1105 22-23 0.540467723839221 1105 24-25 0.4552537603693807 1105 26-27 0.39467234609640656 1105 28-29 0.3730885645908515 1105 30-31 0.3312724313703157 1105 32-33 0.3343339904135405 1105 34-35 0.34661499275530616 1105 36-37 0.400145961574502 1105 38-39 0.3980717489002146 1105 40-41 0.39223915517781194 1105 42-43 0.31263549893277087 1105 44-45 0.3685892618654236 1105 46-47 0.5027097859908416 1105 48-49 0.4182456433521935 1105 50-51 0.29316769054105407 1105 52-53 0.3137797728349625 1105 54-55 0.3123613473746687 1105 56-57 0.40164119888115835 1105 58-59 0.3871595180749523 1105 60-61 0.35934786387712236 1105 62-63 0.5428617919926815 1105 64-65 0.3814205105637818 1105 66-67 0.37179048013144467 1105 68-69 0.3861978016766585 1105 70-71 0.3786803754897967 1105 72-73 0.37419407276890837 1105 74-75 0.5202486413509639 1105 76-77 0.4937371206359167 1105 78-79 0.45669852172678205 1105 80-81 0.610437080141029 1105 82-83 0.5642124495182195 1105 84-85 0.7206213534928807 1105 86-87 0.6183492491933293 1105 88-89 0.540772334894573 1105 90-91 0.4995274344955014 1105 92-93 0.5191260177354557 1105 94-95 0.5677882912269467 1105 96-97 0.5944233049628558 1105 98-99 0.56517686416543 1105 100-101 0.5641372233811239 1106 1 -0.12278859635366501 1106 2 -0.002695493911353708 1106 3 -0.04976519099631105 1106 4 -0.006598400041987418 1106 5 -0.031100402000191707 1106 6 -0.0347805333475506 1106 7 -0.0745266237049762 1106 8 -0.06622524088423631 1106 9 -0.07131015022815035 1106 10-11 -0.14699393168604047 1106 12-13 -0.1687354600425408 1106 14-15 -0.24994175789691297 1106 16-17 -0.16214728217226337 1106 18-19 -0.16984735491055858 1106 20-21 -0.17214769251340556 1106 22-23 -0.047598909784881016 1106 24-25 -0.1571381632099218 1106 26-27 -0.2756193412893353 1106 28-29 -0.1040087271884218 1106 30-31 -0.13106930234201286 1106 32-33 -0.15147948181902393 1106 34-35 -0.1784261780772738 1106 36-37 -0.17485259515563456 1106 38-39 -0.2237277647550826 1106 40-41 -0.22618384264115576 1106 42-43 -0.22618738895592827 1106 44-45 -0.20045921018710544 1106 46-47 -0.09942512855166896 1106 48-49 -0.15593924325297337 1106 50-51 -0.42028197599119466 1106 52-53 -0.42150304603109845 1106 54-55 -0.5404396799563358 1106 56-57 -0.37298374443921034 1106 58-59 -0.3457223691564941 1106 60-61 -0.39800856193295076 1106 62-63 -0.26222154191723135 1106 64-65 -0.34199827628104984 1106 66-67 -0.3449434837329548 1106 68-69 -0.36376337392817604 1106 70-71 -0.4608335002937629 1106 72-73 -0.4910238464906769 1106 74-75 -0.2923114057403673 1106 76-77 -0.2421507611069913 1106 78-79 -0.21590725372100295 1106 80-81 -0.14012086504047616 1106 82-83 -0.1513044160800554 1106 84-85 0.08711340789092858 1106 86-87 -0.0815510649390454 1106 88-89 -0.29095976215715424 1106 90-91 -0.3331901533496371 1106 92-93 -0.3596316669321844 1106 94-95 -0.32633250529901403 1106 96-97 -0.27885172686041315 1106 98-99 -0.20830175810034746 1106 100-101 -0.25449029379894483 1107 1 -0.7578263367764961 1107 2 -0.5726164475890876 1107 3 -0.9146436640052151 1107 4 -0.8577141507185502 1107 5 -0.8691762608551841 1107 6 -0.7094050873123834 1107 7 -0.6168835642399983 1107 8 -0.6208903555381369 1107 9 -0.7877766102517612 1107 10-11 -0.8285518333434894 1107 12-13 -0.8313228561387049 1107 14-15 -1.0357987081289224 1107 16-17 -1.1086839661024328 1107 18-19 -1.1557683076311633 1107 20-21 -1.154998694706542 1107 22-23 -1.0316395005400665 1107 24-25 -1.2223644112802319 1107 26-27 -1.1600146166924006 1107 28-29 -1.254888786397892 1107 30-31 -1.2205547594514528 1107 32-33 -1.2785017197165658 1107 34-35 -1.3074508752096001 1107 36-37 -1.209313945891104 1107 38-39 -1.268661729534081 1107 40-41 -1.316102833110378 1107 42-43 -1.4555891993426755 1107 44-45 -1.3612921989672024 1107 46-47 -1.2025140297467836 1107 48-49 -1.518032419817935 1107 50-51 -1.6488777246627713 1107 52-53 -1.5429112887972423 1107 54-55 -1.4908477740045782 1107 56-57 -1.3446490776044868 1107 58-59 -1.412918904455875 1107 60-61 -1.3970663072343612 1107 62-63 -1.2273031174443823 1107 64-65 -1.3597561662697863 1107 66-67 -1.453798028461815 1107 68-69 -1.4568039183690793 1107 70-71 -1.4327188001271516 1107 72-73 -1.4577977477998836 1107 74-75 -1.3702853643251238 1107 76-77 -1.388917705694272 1107 78-79 -1.6515109334160627 1107 80-81 -1.5081582489685523 1107 82-83 -1.532673733314443 1107 84-85 -1.3780448547999669 1107 86-87 -1.4378962327424851 1107 88-89 -1.5206115379505185 1107 90-91 -1.6772748745766144 1107 92-93 -1.918844574994509 1107 94-95 -1.8526874801038282 1107 96-97 -1.9451333205191688 1107 98-99 -1.984757693835597 1107 100-101 -2.0557835599768595 1201 1 0.88078210082395 1201 2 0.9123463884102847 1201 3 0.9160014766941664 1201 4 0.9420947045946022 1201 5 0.9279284958126723 1201 6 0.93329085615499 1201 7 0.8610956109150862 1201 8 0.8511005885446394 1201 9 1.0232081258151666 1201 10-11 1.0253848637812126 1201 12-13 1.0486709076119922 1201 14-15 1.1426335268438947 1201 16-17 1.2268665952262552 1201 18-19 1.1506679234235122 1201 20-21 1.1523358825310126 1201 22-23 1.2975553748528341 1201 24-25 1.2135298775637793 1201 26-27 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-3.7759589564798866 2307 70-71 -3.730568704383902 2307 72-73 -3.750432199952339 2307 74-75 -3.6483805216459757 2307 76-77 -3.665264593695216 2307 78-79 -3.689526765025299 2307 80-81 -3.445421945147441 2307 82-83 -3.5001807703319514 2307 84-85 -3.236087897634551 2307 86-87 -3.2739478221969343 2307 88-89 -3.4292527665655825 2307 90-91 -3.4744054864792595 2307 92-93 -3.437260562968575 2307 94-95 -3.5360574866754817 2307 96-97 -3.5452871340304775 2307 98-99 -3.5070425494124393 2307 100-101 -3.3379357129357174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1424574.0 3 306821.0 4 251901.0 5 266214.0 6 333507.0 7 472525.0 8 682951.0 9 987090.0 10 1149830.0 11 993642.0 12 692225.0 13 537058.0 14 455024.0 15 410754.0 16 390946.0 17 388932.0 18 402489.0 19 404957.0 20 431631.0 21 458271.0 22 499806.0 23 551613.0 24 625880.0 25 602126.0 26 607770.0 27 670814.0 28 745793.0 29 842468.0 30 966655.0 31 1133940.0 32 1361276.0 33 1677432.0 34 2147740.0 35 2924052.0 36 4360100.0 37 7614012.0 38 1.044885E7 39 3790035.0 40 6780.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.26299978609708 16.90608453246217 8.105821571804427 42.725094109636316 2 15.419918750849085 16.851975459329108 53.83908887190678 13.889016917915026 3 19.232775853549814 21.095484592602602 37.2055059160622 22.466233637785383 4 20.19397275079951 25.033357418009427 35.980391240504325 18.79227859068674 5 20.487692625291988 28.588312282661597 35.99936109971514 14.924633992331271 6 16.850551520388763 33.03756381315098 36.60243953397805 13.50944513248221 7 14.00616458352747 19.185257796930753 53.69600292872132 13.112574690820455 8 12.537711410981059 23.82851366915058 47.7114111700169 15.922363749851456 9 14.23716307971737 22.26070147968276 47.000818071290865 16.50131736930901 10-11 17.058243579637875 31.475724176701558 37.56094688826554 13.90508535539503 12-13 17.096895604233037 25.29819506703546 41.50714559724268 16.097763731488822 14-15 17.23626844151729 26.93140232498311 40.56574328059972 15.266585952899883 16-17 18.378602915756893 27.404286709868074 38.64655829456162 15.57055207981341 18-19 18.600457517010728 27.690167243974873 37.95056445592412 15.758810783090272 20-21 18.83516398341368 28.83361540438747 36.558825462165615 15.772395150033244 22-23 20.120706801962722 28.836911440972777 35.45265041276893 15.589731344295569 24-25 18.482715779518546 30.13580624821513 34.640429323619 16.74104864864732 26-27 18.158131880251865 31.498088217186176 34.09212191754697 16.251657985014987 28-29 19.030602718734652 31.13654489876548 33.1914748423244 16.641377540175466 30-31 19.530265694566094 30.493848754568305 33.10721200136466 16.868673549500947 32-33 18.87090625747974 30.252973194415457 33.576570436472004 17.2995501116328 34-35 19.113157348761174 31.59267877469201 32.342518844393105 16.95164503215371 36-37 18.341867280024733 31.234441628402408 33.269624943456776 17.154066148116076 38-39 18.381307274483202 29.949803620074267 33.818547697211 17.850341408231525 40-41 19.426277417017463 30.14205959564006 33.120224091516015 17.31143889582646 42-43 19.671694135904385 29.96644922889404 33.66501897424231 16.69683766095927 44-45 20.10841545577727 29.56155413213707 33.0355430857556 17.294487326330067 46-47 18.341618844357214 30.903361687352422 32.74484282218861 18.010176646101762 48-49 18.20978266365694 30.322014723024154 32.702181492243305 18.7660211210756 50-51 18.53040058188748 30.4650080011038 33.05931641275052 17.945275004258193 52-53 18.18261754453395 31.228416242088713 33.29199977319123 17.29696644018611 54-55 18.06169319122789 31.54449783674917 31.974912796056763 18.418896175966175 56-57 18.544885213994426 31.43805186885389 32.89898858669743 17.11807433045426 58-59 17.895875710063684 32.77721218886606 32.10758723316193 17.21932486790833 60-61 18.168188286937816 33.80595435357978 30.61494836404602 17.410908995436383 62-63 18.297203842128003 34.31448015671742 30.318661415504273 17.069654585650305 64-65 18.30664051918121 33.328100252586445 30.811493897434726 17.553765330797614 66-67 18.24299789908091 33.69559707620718 30.513680022312982 17.547725002398927 68-69 18.931611414614455 32.86152370383195 30.76346739124961 17.443397490303987 70-71 18.247440443143805 32.735484276182696 30.762880585637586 18.254194695035906 72-73 18.332292561168657 32.931106390227924 30.749315595165505 17.987285453437913 74-75 18.881485648793692 32.10404905725983 30.766121474405537 18.248343819540942 76-77 18.319647259454594 32.69673670293705 30.85919962473126 18.124416412877096 78-79 18.364728182009788 33.37707426665949 30.537063146332578 17.721134404998146 80-81 18.606749730466436 33.41587002092838 30.417041858434057 17.560338390171136 82-83 18.8299933283645 33.25472584240621 30.33482341743306 17.580457411796232 84-85 18.59309713791827 33.213526032964516 30.211132829327862 17.982243999789358 86-87 18.70809774486732 33.39271797641415 30.293812475978893 17.605371802739636 88-89 18.844541585620465 33.15577600242452 30.2155610465456 17.784121365409426 90-91 18.34792470989082 33.69803216370798 29.99246289377292 17.96158023262829 92-93 19.251365281247747 32.91351980200074 30.249436138938986 17.585678777812525 94-95 19.32463310758245 33.28302602313824 29.50689298452232 17.885447884756996 96-97 19.00809630844971 33.706373988362245 29.924858847340865 17.36067085584718 98-99 19.614821190510845 33.045299103046496 29.559254325478708 17.780625380963958 100-101 19.97443287891823 33.82169320420403 28.7417865437269 17.46208737315084 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31635.0 1 33252.0 2 33647.0 3 38414.5 4 51423.5 5 68799.5 6 86654.5 7 103619.5 8 122672.0 9 142078.5 10 161578.0 11 179887.0 12 197928.0 13 216397.5 14 238275.0 15 259975.0 16 277563.5 17 299339.0 18 329550.0 19 363267.0 20 406484.0 21 469853.5 22 551788.0 23 635494.5 24 741169.5 25 887265.5 26 1021374.5 27 1165641.5 28 1314368.5 29 1473090.5 30 1653305.0 31 1758246.0 32 1840225.5 33 1964875.5 34 2094782.0 35 2135299.0 36 2211242.0 37 2338937.0 38 2374501.5 39 2302126.5 40 2283588.5 41 2275852.5 42 1961837.5 43 1617500.5 44 1442286.5 45 1321927.0 46 1250110.0 47 1170895.0 48 1084716.5 49 1008383.5 50 884479.0 51 730389.0 52 610407.5 53 523342.5 54 444969.0 55 373655.0 56 312516.5 57 257260.0 58 213320.5 59 176029.5 60 133382.5 61 95036.0 62 71712.0 63 55778.5 64 41600.5 65 29187.0 66 20064.0 67 14240.0 68 10117.5 69 7238.5 70 5532.5 71 4551.5 72 3953.5 73 3553.0 74 3092.5 75 2501.0 76 2204.0 77 1970.0 78 1713.0 79 1466.5 80 1226.0 81 1015.0 82 839.0 83 673.0 84 506.5 85 414.0 86 347.0 87 270.5 88 203.5 89 154.5 90 105.0 91 63.0 92 41.5 93 23.5 94 14.0 95 11.0 96 5.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12175942261947739 2 0.14919325116878107 3 0.12980567305545743 4 0.11222312580646401 5 0.11077834666113803 6 0.19142757835173108 7 0.17283029065863145 8 0.18202142482987632 9 0.24185527447370997 10-11 0.22994904946735178 12-13 0.23419190937258785 14-15 0.2614484412643711 16-17 0.2909070353652511 18-19 0.31066806814016035 20-21 0.3024388626426965 22-23 0.30082150217648623 24-25 0.3003565699841587 26-27 0.27385260581951004 28-29 0.24981381964825702 30-31 0.2433717267359059 32-33 0.276279118052489 34-35 0.29350424278445986 36-37 0.2947424901851211 38-39 0.29237067585712184 40-41 0.3005847922773499 42-43 0.2831116408383159 44-45 0.3046805336795371 46-47 0.30680149209849156 48-49 0.08755154145863545 50-51 0.016829979521858832 52-53 0.008793159759151167 54-55 0.006128994559708647 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.430673272362899E-7 64-65 2.82920198170887E-6 66-67 4.0551895071160465E-5 68-69 5.8470174288649974E-5 70-71 5.469790497970481E-5 72-73 2.9423700609772246E-4 74-75 5.941324161588626E-5 76-77 9.034584994923658E-4 78-79 4.875658081811619E-4 80-81 7.733152083337577E-5 82-83 0.0 84-85 2.263361585367096E-5 86-87 1.320294258130806E-5 88-89 2.1784855259158296E-4 90-91 2.3670989913630874E-4 92-93 1.9615800406514833E-4 94-95 2.451975050814354E-5 96-97 0.0 98-99 3.206428912603386E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.3018484E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.489536531480816 #Duplication Level Percentage of deduplicated Percentage of total 1 82.26730909532174 26.728267441990795 2 7.6874445702855 4.995230224000491 3 2.837623541424483 2.7657922113510214 4 1.5464515173089235 2.0097397226308886 5 0.9733094321120433 1.581118617551954 6 0.7289173260056409 1.4209311655013748 7 0.5130353576855692 1.166779669682664 8 0.3857270063949096 1.0025673332356921 9 0.29770179373334316 0.8704973972688138 >10 2.235255815260431 14.353968053072888 >50 0.2780997161873187 6.319244259822352 >100 0.21227204636103517 13.65207188555678 >500 0.02056809794274628 4.580041378040105 >1k 0.014013312624648494 8.886978124289973 >5k 0.0014588967240166915 3.251345008318096 >10k+ 8.124746276866232E-4 6.415427507686203 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 346861 0.6542265523850135 Illumina Single End PCR Primer 1 (100% over 50bp) CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 142503 0.26877984666630605 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 102349 0.19304399575061407 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 97237 0.18340207539695025 Illumina Single End PCR Primer 1 (100% over 50bp) CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 86867 0.1638428590300696 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73533 0.13869313954733223 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 70532 0.13303284944926 No Hit CAAGCAGAAGACGGCATACGAGATCTGAAGAGCGTCGTGTAGGGAAAGAG 68854 0.12986791549905502 Illumina Single End PCR Primer 1 (96% over 30bp) GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 59295 0.11183835433695162 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 57325 0.10812266906764063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.9154147070670674 0.0 0.0 2.6405885162616116E-5 0.0 2 0.9200791180675781 1.8861346544725798E-6 0.0 3.0178154471561276E-5 0.0 3 0.9201771970696107 1.8861346544725798E-6 0.0 4.1494962398396754E-5 0.0 4 0.9662648973516481 1.8861346544725798E-6 0.0 4.9039501016287074E-5 0.0 5 0.9674852264730919 1.8861346544725798E-6 0.0 5.281177032523223E-5 0.0 6 0.9684546996854908 1.8861346544725798E-6 0.0 6.790084756101287E-5 0.0 7 0.9724136963252288 1.8861346544725798E-6 0.0 8.298992479679351E-5 0.0 8 1.005270162006141 1.8861346544725798E-6 0.0 8.676219410573866E-5 0.0 9 1.0058774973648812 1.8861346544725798E-6 0.0 1.1316807926835479E-4 0.0 10-11 1.0195246246573175 1.8861346544725798E-6 0.0 1.1599728125006366E-4 0.0 12-13 1.025109469369211 1.8861346544725798E-6 0.0 1.3202942581308058E-4 1.8861346544725798E-6 14-15 1.0278226740696699 1.8861346544725798E-6 0.0 1.584353109756967E-4 1.8861346544725798E-6 16-17 1.0301228152807989 1.8861346544725798E-6 0.0 1.7258132088424105E-4 1.8861346544725798E-6 18-19 1.0321598407076293 1.8861346544725798E-6 0.0 1.9993027337409345E-4 1.8861346544725798E-6 20-21 1.0434342106047394 1.8861346544725798E-6 0.0 2.4048216844525393E-4 1.8861346544725798E-6 22-23 1.0553102574566258 3.7722693089451595E-6 0.0 2.508559090448531E-4 1.8861346544725798E-6 24-25 1.0579206678184159 4.71533663618145E-6 0.0 2.876355348070684E-4 1.8861346544725798E-6 26-27 1.0609733767566798 5.65840396341774E-6 0.0 4.800212695632715E-4 1.8861346544725798E-6 28-29 1.0642184714296998 5.65840396341774E-6 0.0 6.129937627035885E-4 1.8861346544725798E-6 30-31 1.0679699932574458 5.65840396341774E-6 0.0 6.299689745938417E-4 1.8861346544725798E-6 32-33 1.0721477815171028 1.2259875254071769E-5 0.0 6.384565805389683E-4 1.8861346544725798E-6 34-35 1.0762944485549606 1.5089077235780638E-5 0.0 6.526025904475127E-4 1.8861346544725798E-6 36-37 1.0819019268827077 1.5089077235780638E-5 0.0 6.837238122463102E-4 1.8861346544725798E-6 38-39 1.0878517386502413 1.5089077235780638E-5 0.0 6.95983687500382E-4 1.8861346544725798E-6 40-41 1.095086951184798 1.6975211890253217E-5 0.0 7.478523904983779E-4 1.8861346544725798E-6 42-43 1.113055212970631 1.6975211890253217E-5 0.0 8.119809687504457E-4 1.8861346544725798E-6 44-45 1.1205686303667228 1.88613465447258E-5 0.0 8.412160558947706E-4 1.8861346544725798E-6 46-47 1.1500790931706006 2.0747481199198377E-5 0.0 9.157183747464375E-4 1.8861346544725798E-6 48-49 1.1637799753006894 2.0747481199198377E-5 0.0 9.515549331814165E-4 1.8861346544725798E-6 50-51 1.1804901852719893 2.734895248985241E-5 1.8861346544725798E-6 9.666440104171971E-4 2.8292019817088694E-6 52-53 1.219106906187661 3.0178154471561276E-5 1.8861346544725798E-6 9.741885490350874E-4 4.71533663618145E-6 54-55 1.2365696838860951 3.0178154471561276E-5 1.8861346544725798E-6 9.82676154980214E-4 5.65840396341774E-6 56-57 1.2495604363187751 3.0178154471561276E-5 1.8861346544725798E-6 9.89277626270868E-4 5.65840396341774E-6 58-59 1.2650720077171576 3.0178154471561276E-5 1.8861346544725798E-6 0.001005309770833885 5.65840396341774E-6 60-61 1.2794839626119827 3.1121221798797565E-5 3.7722693089451595E-6 0.0010166265787607204 5.65840396341774E-6 62-63 1.3024740579153489 3.2064289126033855E-5 3.7722693089451595E-6 0.0010534062045229357 5.65840396341774E-6 64-65 1.320542284837869 3.2064289126033855E-5 3.7722693089451595E-6 0.001099616503557514 5.65840396341774E-6 66-67 1.3402429613038351 3.2064289126033855E-5 3.7722693089451595E-6 0.0011439406679376197 5.65840396341774E-6 68-69 1.3633528261577603 3.395042378050643E-5 3.7722693089451595E-6 0.001155257475864455 5.65840396341774E-6 70-71 1.387588713400406 3.9608827743924175E-5 3.7722693089451595E-6 0.0011694034857729994 5.65840396341774E-6 72-73 1.4151347669616505 3.9608827743924175E-5 3.7722693089451595E-6 0.001180720293699835 5.65840396341774E-6 74-75 1.4477771563592805 3.9608827743924175E-5 3.7722693089451595E-6 0.0012156137848075778 5.65840396341774E-6 76-77 1.4837014200556924 3.9608827743924175E-5 3.7722693089451595E-6 0.0016098159275923468 5.65840396341774E-6 78-79 1.5253651915056643 4.1494962398396754E-5 3.7722693089451595E-6 0.0017003503910070308 5.65840396341774E-6 80-81 1.5741764702287604 4.243802972563304E-5 3.7722693089451595E-6 0.0018003155276940775 5.65840396341774E-6 82-83 1.632162850978538 4.338109705286933E-5 3.7722693089451595E-6 0.0018672733079278538 6.60147129065403E-6 84-85 1.7016197596294909 4.338109705286933E-5 3.7722693089451595E-6 0.0018748178465457443 7.544538617890319E-6 86-87 1.786113876813226 4.526723170734192E-5 3.7722693089451595E-6 0.0018851915871453437 7.544538617890319E-6 88-89 1.887807655911097 4.7153366361814496E-5 3.7722693089451595E-6 0.0019002806643811242 7.544538617890319E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGGC 30610 0.0 67.945595 9 CGCGTGT 48490 0.0 64.47181 1 AAGACGG 35030 0.0 60.49792 8 GCGTGTT 62615 0.0 49.890415 2 GAAGACG 45040 0.0 47.17873 7 ATCGGGA 10845 0.0 42.289215 2 GAGCGGC 13090 0.0 41.89236 9 GTTTTTT 936770 0.0 40.388466 1 TCGGGAG 11775 0.0 37.62986 3 CGGCATA 27180 0.0 35.84776 12-13 CGCCGTA 83615 0.0 35.318024 46-47 CAAGCAG 59225 0.0 35.05143 1 CCGTATC 84915 0.0 34.94518 48-49 ACGGCAT 28580 0.0 34.26889 10-11 GTCGCCG 87560 0.0 34.104145 44-45 AGAGCGG 16805 0.0 33.9532 8 CCGAGAT 12165 0.0 33.559017 1 AGAGCGT 138530 0.0 33.40177 9 GACGGCA 29995 0.0 32.99323 10-11 CCCGAAA 10070 0.0 32.98951 1 >>END_MODULE