FastQCFastQC Report
Fri 27 May 2016
SRR1233044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53018484
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC5202650.9812898460091767TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG3782090.7133531015334199TruSeq Adapter, Index 9 (100% over 49bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1043850.19688416590712024No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT926750.17479752910324634No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA835920.1576657680366719No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC740890.13974183041521895No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT724090.13657312419570503No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA660320.12454524350413339No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC622840.11747601081917017No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552730.10425232075666291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT488700.070.992721
GCGTGTT628600.055.7512172
CGGTGGT61450.045.216871
GTTTTTT8807850.039.5569421
CCCGAAA83150.036.964071
CGGCCTT57200.034.5192681
CGGAAGA2041750.034.381974
CGTGTTT1056650.033.6920553
TCGGAAG2005250.033.5301253
ATCGGAA2045850.033.3476072
GATCGGA2054050.032.954281
CCGAGAT80100.032.13471
GTGTTTT2606900.031.8059061
GAGCACA2233850.031.6888839
AGATCGG1455200.031.5576131
AGAGCAC2308200.030.7771848
CTCGTAT1792300.030.10361342-43
CCGAAAC101400.029.737752
GAAGAGC2427350.029.3231756
ACGATCA2064800.028.99976532-33
Produced by FastQC (version 0.11.5)