##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233044_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53018484 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.912493669189033 31.0 30.0 34.0 25.0 34.0 2 30.60411959346103 33.0 31.0 34.0 26.0 34.0 3 30.086548513910735 33.0 30.0 34.0 25.0 34.0 4 33.45547394376648 37.0 35.0 37.0 27.0 37.0 5 33.58182519892496 37.0 35.0 37.0 28.0 37.0 6 33.71827025457763 37.0 35.0 37.0 29.0 37.0 7 33.88285696739273 37.0 35.0 37.0 30.0 37.0 8 33.90086165043874 37.0 35.0 37.0 30.0 37.0 9 35.369273270808726 39.0 35.0 39.0 30.0 39.0 10-11 35.16835055110214 39.0 35.0 39.0 29.0 39.0 12-13 34.98699652558908 39.0 35.0 39.0 28.0 39.0 14-15 36.137218003064746 40.0 36.0 41.0 27.0 41.0 16-17 35.96037078314046 40.0 36.0 41.0 27.0 41.0 18-19 35.52076313611683 40.0 35.5 41.0 26.0 41.0 20-21 35.06065756237014 40.0 35.0 41.0 23.0 41.0 22-23 34.76162590767401 40.0 35.0 41.0 18.5 41.0 24-25 34.41609259329256 40.0 35.0 41.0 13.5 41.0 26-27 33.90109725694911 40.0 34.0 41.0 5.0 41.0 28-29 33.47687135867559 40.0 34.0 41.0 2.0 41.0 30-31 33.074165426910355 39.0 33.0 41.0 2.0 41.0 32-33 32.77181159121788 39.0 33.0 41.0 2.0 41.0 34-35 32.5554075820048 39.0 32.5 41.0 2.0 41.0 36-37 32.39923098329254 39.0 32.0 41.0 2.0 41.0 38-39 32.30890679559981 39.0 32.0 41.0 2.0 41.0 40-41 32.18384768413974 39.0 32.0 41.0 2.0 41.0 42-43 31.92851659055359 39.0 31.0 41.0 2.0 41.0 44-45 31.85811685034223 39.0 31.0 41.0 2.0 41.0 46-47 31.690384281828955 38.0 31.0 40.0 2.0 41.0 48-49 31.52084417389226 38.0 31.0 40.0 2.0 41.0 50-51 31.283433915236053 38.0 30.0 40.0 2.0 41.0 52-53 30.97887296249361 38.0 30.0 40.0 2.0 41.0 54-55 30.785850129173816 38.0 30.0 40.0 2.0 41.0 56-57 30.652790892700743 38.0 29.0 40.0 2.0 41.0 58-59 30.454228010367103 38.0 29.0 40.0 2.0 41.0 60-61 30.18130095911456 37.0 28.0 40.0 2.0 41.0 62-63 29.865721971605225 37.0 28.0 40.0 2.0 41.0 64-65 29.423144124603787 36.0 26.5 39.5 2.0 41.0 66-67 29.078674175217834 36.0 26.0 39.0 2.0 40.0 68-69 28.664963798285896 35.5 26.0 39.0 2.0 40.0 70-71 28.06890822264929 35.0 23.5 38.5 2.0 40.0 72-73 27.52219829597542 34.5 21.5 38.0 2.0 40.0 74-75 27.12778975347541 34.0 20.5 37.0 2.0 39.5 76-77 26.335943234438766 33.5 19.5 36.0 2.0 39.0 78-79 26.44983389189325 34.0 18.5 36.0 2.0 39.0 80-81 26.207658710120796 34.0 18.0 36.0 2.0 38.5 82-83 25.8589453727119 34.0 14.5 35.5 2.0 37.0 84-85 25.433125398304487 33.0 7.5 35.0 2.0 37.0 86-87 24.988797491833225 33.0 4.5 35.0 2.0 36.0 88-89 24.57339051791824 32.0 2.0 35.0 2.0 36.0 90-91 24.166190860908056 32.0 2.0 34.0 2.0 35.5 92-93 23.717927666509667 31.5 2.0 34.0 2.0 35.0 94-95 23.311453407456916 31.0 2.0 34.0 2.0 35.0 96-97 22.86887280669889 31.0 2.0 34.0 2.0 35.0 98-99 22.20938548525831 31.0 2.0 34.0 2.0 35.0 100-101 19.59870979147574 27.0 2.0 32.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5731511106497607 1101 2 1.2275809952549253 1101 3 1.7727187590893791 1101 4 1.7355159460197882 1101 5 1.4294805375168806 1101 6 1.234997966477799 1101 7 1.1058379068077429 1101 8 1.0798946458579834 1101 9 1.2531347279936043 1101 10-11 1.2957313779635555 1101 12-13 1.4048386651166567 1101 14-15 1.5467833840088687 1101 16-17 1.4989903583798139 1101 18-19 1.5725779932913966 1101 20-21 1.6144296706818722 1101 22-23 1.478619759352398 1101 24-25 1.5015776175534867 1101 26-27 1.5353388171009072 1101 28-29 1.5412777120185908 1101 30-31 1.5648964379342019 1101 32-33 1.5448781902049333 1101 34-35 1.6059628438792757 1101 36-37 1.6151417199467986 1101 38-39 1.5998997641125783 1101 40-41 1.6052629077081733 1101 42-43 1.585489781670816 1101 44-45 1.6611812512181388 1101 46-47 1.5813814862600104 1101 48-49 1.5368842907312938 1101 50-51 1.5700195356964244 1101 52-53 1.6685769242372999 1101 54-55 1.715761366346765 1101 56-57 1.6776126350841203 1101 58-59 1.6670616958008182 1101 60-61 1.621768971870182 1101 62-63 1.6263686528784973 1101 64-65 1.6085467959038482 1101 66-67 1.5782625466802678 1101 68-69 1.5272213611319678 1101 70-71 1.5003127027273884 1101 72-73 1.611221520009007 1101 74-75 1.4932937390288537 1101 76-77 1.477687439433769 1101 78-79 1.5769479196826488 1101 80-81 1.5263812238138783 1101 82-83 1.5160195485746435 1101 84-85 1.4609629149640462 1101 86-87 1.44565962436738 1101 88-89 1.5496351934122323 1101 90-91 1.516297099136338 1101 92-93 1.4811943302065664 1101 94-95 1.4043874402568797 1101 96-97 1.4865752124918181 1101 98-99 1.4699468168514045 1101 100-101 1.658811889501255 1102 1 1.3714040325003474 1102 2 1.0248853270592662 1102 3 1.5809132008924998 1102 4 1.5418837185278278 1102 5 1.2014652248997209 1102 6 0.9998091639792648 1102 7 0.8953410531870745 1102 8 0.8502201592174288 1102 9 0.9881308914795923 1102 10-11 1.0547846662326563 1102 12-13 1.1599000690702752 1102 14-15 1.2259453077011457 1102 16-17 1.1936101069613159 1102 18-19 1.2183121667007555 1102 20-21 1.2250497557491045 1102 22-23 1.1391692277271517 1102 24-25 1.1946548735389655 1102 26-27 1.2495535897810655 1102 28-29 1.2870859929044514 1102 30-31 1.2777787485185215 1102 32-33 1.2601993754845324 1102 34-35 1.2554837986439153 1102 36-37 1.2740709702877595 1102 38-39 1.2936085610634436 1102 40-41 1.2378043048044276 1102 42-43 1.2643088374347613 1102 44-45 1.332274064748141 1102 46-47 1.3267916167829874 1102 48-49 1.3346597897990549 1102 50-51 1.3185516279718072 1102 52-53 1.3739327711793194 1102 54-55 1.404793308456771 1102 56-57 1.3800441886570134 1102 58-59 1.3775890116167133 1102 60-61 1.3584938619898566 1102 62-63 1.3686564579488483 1102 64-65 1.3525092372598593 1102 66-67 1.387306648332526 1102 68-69 1.3212236044552483 1102 70-71 1.2807236944501703 1102 72-73 1.3793591740846303 1102 74-75 1.2675383589498175 1102 76-77 1.229329925046219 1102 78-79 1.355883083518048 1102 80-81 1.3415474695593943 1102 82-83 1.2860042644601215 1102 84-85 1.2473935316946019 1102 86-87 1.2653524521484876 1102 88-89 1.3743953795319932 1102 90-91 1.3306934477965129 1102 92-93 1.2702905974175636 1102 94-95 1.266979384336917 1102 96-97 1.3621653312963495 1102 98-99 1.3285730512039535 1102 100-101 1.5293327749921914 1103 1 1.197502272299598 1103 2 0.8776555329666174 1103 3 1.4416738808034069 1103 4 1.4145873613688948 1103 5 1.0387072158799597 1103 6 0.8819744869818109 1103 7 0.7477251089152048 1103 8 0.723376968231527 1103 9 0.807073123551973 1103 10-11 0.84960041664133 1103 12-13 0.9503403577121787 1103 14-15 1.0466177078738639 1103 16-17 1.009025511726577 1103 18-19 1.033078220287102 1103 20-21 1.0858290829656099 1103 22-23 1.066057456486405 1103 24-25 1.0206448652521445 1103 26-27 1.0337252879047938 1103 28-29 1.024238071554116 1103 30-31 1.0466488082730123 1103 32-33 1.0332481528174853 1103 34-35 1.0585857824101481 1103 36-37 1.0271427270238647 1103 38-39 1.0547852470577368 1103 40-41 1.0481524119757495 1103 42-43 1.054821286643005 1103 44-45 1.130774963057327 1103 46-47 1.110462694370458 1103 48-49 1.087201988525699 1103 50-51 1.1050139220002535 1103 52-53 1.1678021494533581 1103 54-55 1.1772858150019196 1103 56-57 1.0830350816391388 1103 58-59 1.105886405163325 1103 60-61 1.1036621482819164 1103 62-63 1.1022084069987024 1103 64-65 1.1600115250749532 1103 66-67 1.1768901549227344 1103 68-69 1.1121928473249234 1103 70-71 1.0950350779939662 1103 72-73 1.192486933656884 1103 74-75 1.0684873675874194 1103 76-77 1.0112291356822816 1103 78-79 1.1574791026972235 1103 80-81 1.0438748563942681 1103 82-83 1.0376122831793104 1103 84-85 0.9935864506448198 1103 86-87 1.0285390225130868 1103 88-89 1.1170673479784519 1103 90-91 0.9972879766291172 1103 92-93 0.9542884349785723 1103 94-95 0.9640393530573483 1103 96-97 0.999573457611266 1103 98-99 0.9912569077912821 1103 100-101 1.1721388151644767 1104 1 1.0865704390116342 1104 2 0.7635846121510355 1104 3 1.3017384978961495 1104 4 1.3063974995748495 1104 5 0.8994323397965474 1104 6 0.7321810117007601 1104 7 0.6698032304548818 1104 8 0.6087456470844117 1104 9 0.6600376254195268 1104 10-11 0.6613278236253777 1104 12-13 0.7660105359331197 1104 14-15 0.831356975850376 1104 16-17 0.8231991550776101 1104 18-19 0.8677893319783507 1104 20-21 0.9029217708547677 1104 22-23 0.8230509122441703 1104 24-25 0.7921798564154443 1104 26-27 0.7921253910840207 1104 28-29 0.7596009823914898 1104 30-31 0.8025692961082385 1104 32-33 0.8377212419514208 1104 34-35 0.8111325697441387 1104 36-37 0.7859513660887032 1104 38-39 0.7705376493054956 1104 40-41 0.804337281866168 1104 42-43 0.7884838960420382 1104 44-45 0.8189756983169545 1104 46-47 0.8124141182396691 1104 48-49 0.7668118825074686 1104 50-51 0.7883429052096389 1104 52-53 0.9046083881157827 1104 54-55 0.8420480317248753 1104 56-57 0.8009555116806233 1104 58-59 0.8469770263421523 1104 60-61 0.7864346951822156 1104 62-63 0.7803358737026613 1104 64-65 0.8232461870911933 1104 66-67 0.7800387241712414 1104 68-69 0.7530688197559563 1104 70-71 0.7699284953082426 1104 72-73 0.9136916692360444 1104 74-75 0.7637700704300201 1104 76-77 0.7677639886108416 1104 78-79 0.855853344790706 1104 80-81 0.7648041311876739 1104 82-83 0.7694448063980595 1104 84-85 0.7516788848456635 1104 86-87 0.7728548387306802 1104 88-89 0.7987667604425148 1104 90-91 0.7979889690718238 1104 92-93 0.7225219609756124 1104 94-95 0.7384483938767268 1104 96-97 0.7648282573607688 1104 98-99 0.6347601012183155 1104 100-101 0.8523254352925989 1105 1 0.8240923026922324 1105 2 0.5516725609354296 1105 3 1.1080597889309693 1105 4 1.1529191639408722 1105 5 0.6965806523613907 1105 6 0.49271456903771593 1105 7 0.4467386506462532 1105 8 0.3966748134569045 1105 9 0.3879040830114775 1105 10-11 0.3759994320526445 1105 12-13 0.45288386770132405 1105 14-15 0.49307423109608095 1105 16-17 0.4855636537019734 1105 18-19 0.5446837304082024 1105 20-21 0.5839523039101593 1105 22-23 0.5224862330085642 1105 24-25 0.480837652613026 1105 26-27 0.4635008541066554 1105 28-29 0.46720629682197057 1105 30-31 0.45334959263704633 1105 32-33 0.4858319813977232 1105 34-35 0.459942123076317 1105 36-37 0.45753577039848636 1105 38-39 0.44889178392287477 1105 40-41 0.44601159411182323 1105 42-43 0.4587404433041513 1105 44-45 0.47821009239682866 1105 46-47 0.49315275167189654 1105 48-49 0.4496814017377062 1105 50-51 0.45377476224868474 1105 52-53 0.5004275707076502 1105 54-55 0.4912413043132524 1105 56-57 0.37068092913069606 1105 58-59 0.45308644378265583 1105 60-61 0.45693693831382376 1105 62-63 0.37662744289145067 1105 64-65 0.49467431829673103 1105 66-67 0.5061229852101405 1105 68-69 0.4706113787186972 1105 70-71 0.4378604056548241 1105 72-73 0.5444443519431346 1105 74-75 0.4072138294171985 1105 76-77 0.39724333086838826 1105 78-79 0.39194738502304816 1105 80-81 0.37676622182967634 1105 82-83 0.35200503402992567 1105 84-85 0.32144234210765177 1105 86-87 0.355541541792892 1105 88-89 0.3774198406557083 1105 90-91 0.33000309895719226 1105 92-93 0.28115607238380846 1105 94-95 0.29140451200663264 1105 96-97 0.278559476429745 1105 98-99 0.16159909260520777 1105 100-101 0.44164753967116965 1106 1 0.5266704056994911 1106 2 0.26455295885654806 1106 3 0.8227870013320882 1106 4 0.8680722540516115 1106 5 0.4058899208619309 1106 6 0.1887670047819654 1106 7 0.14821103076923237 1106 8 0.1267393121317113 1106 9 -0.031280750374861555 1106 10-11 0.004709099970646946 1106 12-13 0.0342995996878841 1106 14-15 -0.022564187358476318 1106 16-17 -0.08469898795104314 1106 18-19 -0.014706560426425597 1106 20-21 0.07877849647848478 1106 22-23 0.033752988720010535 1106 24-25 -0.02067071683958943 1106 26-27 -0.038136614777776856 1106 28-29 -0.05911315490617852 1106 30-31 -0.037828807337028536 1106 32-33 -0.049185887275591256 1106 34-35 -0.11944454070792432 1106 36-37 -0.07293723946478536 1106 38-39 -0.07906342084605456 1106 40-41 -0.080799109309865 1106 42-43 -0.08679592615573739 1106 44-45 -0.04064387548272208 1106 46-47 -0.0447665838507767 1106 48-49 -0.20098545393563683 1106 50-51 -0.21308191102562546 1106 52-53 -0.17181435083858787 1106 54-55 -0.276117051403471 1106 56-57 -0.32361048956277116 1106 58-59 -0.19192241982121772 1106 60-61 -0.16419267180762986 1106 62-63 -0.18445324734517854 1106 64-65 -0.10040846222680955 1106 66-67 -0.05365255134251612 1106 68-69 -0.1537814047259829 1106 70-71 -0.11977338357609213 1106 72-73 -0.1334302638337661 1106 74-75 -0.26198273548647677 1106 76-77 -0.2831988383068662 1106 78-79 -0.07157521385027721 1106 80-81 -0.09484065316939194 1106 82-83 -0.15335402309739266 1106 84-85 -0.18076743154491837 1106 86-87 -0.06352759079990022 1106 88-89 -0.05716782335032278 1106 90-91 -0.07690839410893346 1106 92-93 -0.10356373702814281 1106 94-95 -0.15557488581120893 1106 96-97 -0.1762308266553667 1106 98-99 -0.29205639457451227 1106 100-101 -0.0376573394050439 1107 1 -0.6248711504192563 1107 2 -0.6791911654008409 1107 3 -4.993099013348786 1107 4 -1.8179100798633208 1107 5 -1.3028946118243567 1107 6 -1.016393621033174 1107 7 -0.9251634537141129 1107 8 -0.8294713644060323 1107 9 -0.8833144619597633 1107 10-11 -0.9149355600694946 1107 12-13 -0.8890878403313636 1107 14-15 -1.0472862029581549 1107 16-17 -1.0655263316280212 1107 18-19 -0.9558026786195555 1107 20-21 -0.9052481958736962 1107 22-23 -1.1707683827471982 1107 24-25 -1.2003858842450086 1107 26-27 -1.2605865508346739 1107 28-29 -1.2185871319692083 1107 30-31 -1.207417200394044 1107 32-33 -1.2513813877502145 1107 34-35 -1.3195558220795434 1107 36-37 -1.2602975110985817 1107 38-39 -1.2918602157201562 1107 40-41 -1.2992176249302574 1107 42-43 -1.31466097743019 1107 44-45 -1.1720973566503652 1107 46-47 -1.209993212110458 1107 48-49 -1.2946305602536299 1107 50-51 -1.3173100975787229 1107 52-53 -1.223954066112654 1107 54-55 -1.3097348693156583 1107 56-57 -1.2290918974473541 1107 58-59 -1.2382798930873733 1107 60-61 -1.2089789026050823 1107 62-63 -1.2254657504665332 1107 64-65 -1.3226598993970526 1107 66-67 -1.1961104392709991 1107 68-69 -1.1765629602384955 1107 70-71 -1.1461449050309405 1107 72-73 -1.0753427067894137 1107 74-75 -1.2699807476199076 1107 76-77 -1.2854698110054272 1107 78-79 -1.1057203416007475 1107 80-81 -1.1493586815247454 1107 82-83 -1.2430307113330095 1107 84-85 -1.1996322114915436 1107 86-87 -1.1632883908527276 1107 88-89 -1.2409703996898536 1107 90-91 -1.3241981040092767 1107 92-93 -1.344281213268463 1107 94-95 -1.2747649209000294 1107 96-97 -1.356617251452498 1107 98-99 -1.5822180873111122 1107 100-101 -1.2324759305561024 1201 1 1.5252242344282188 1201 2 1.1903982578872245 1201 3 1.7537191971457915 1201 4 1.6942292575461693 1201 5 1.371155213418298 1201 6 1.190566848580886 1201 7 1.077310271715163 1201 8 1.0445939705839749 1201 9 1.176310640656581 1201 10-11 1.2011924070465412 1201 12-13 1.3128912397832266 1201 14-15 1.4068829615340874 1201 16-17 1.3925827107102577 1201 18-19 1.4306423356992823 1201 20-21 1.473543540380966 1201 22-23 1.3701189058339338 1201 24-25 1.3714987439496085 1201 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2307 5 -6.668087182083365 2307 6 -5.237979896617766 2307 7 -4.4308176298258 2307 8 -4.2695009205651395 2307 9 -4.5990757618760085 2307 10-11 -4.485310095401694 2307 12-13 -4.371560861495851 2307 14-15 -4.793913288855407 2307 16-17 -5.137511795542114 2307 18-19 -4.826127434748592 2307 20-21 -4.777612440844219 2307 22-23 -5.0567596158660635 2307 24-25 -5.049535853887491 2307 26-27 -4.991978692817263 2307 28-29 -4.966054770653983 2307 30-31 -4.953736201032989 2307 32-33 -4.955301093973148 2307 34-35 -5.0011579545439275 2307 36-37 -5.039584372044423 2307 38-39 -5.00471574206729 2307 40-41 -4.943271205506015 2307 42-43 -4.961811099308136 2307 44-45 -5.004499159910651 2307 46-47 -5.016117077748678 2307 48-49 -5.0124468598796454 2307 50-51 -5.05156420140802 2307 52-53 -5.80665497972506 2307 54-55 -5.170204711393776 2307 56-57 -5.02903312954566 2307 58-59 -4.987455809277257 2307 60-61 -4.953215409632069 2307 62-63 -4.8911222788611575 2307 64-65 -4.822358960009769 2307 66-67 -4.829678703787216 2307 68-69 -4.792678090080038 2307 70-71 -4.6351055810980775 2307 72-73 -4.462207574505957 2307 74-75 -4.582212289141449 2307 76-77 -4.8116964699663605 2307 78-79 -4.7954312110562825 2307 80-81 -4.5492154478458176 2307 82-83 -4.623915808370992 2307 84-85 -4.621833849933271 2307 86-87 -4.537312288117519 2307 88-89 -4.610629842020643 2307 90-91 -4.580296195409979 2307 92-93 -4.687581205751449 2307 94-95 -4.6106048448914905 2307 96-97 -4.46120167327695 2307 98-99 -4.379839543840411 2307 100-101 -3.673233128053102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1667371.0 3 436619.0 4 308381.0 5 324469.0 6 590966.0 7 554481.0 8 481917.0 9 692605.0 10 1006638.0 11 947891.0 12 668646.0 13 478490.0 14 403958.0 15 376754.0 16 364416.0 17 354981.0 18 355300.0 19 361640.0 20 377106.0 21 398815.0 22 434175.0 23 472063.0 24 525481.0 25 596249.0 26 690980.0 27 728103.0 28 694279.0 29 798427.0 30 948253.0 31 1141858.0 32 1390731.0 33 1780955.0 34 2406613.0 35 3511188.0 36 5879361.0 37 1.0499221E7 38 8723989.0 39 645087.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.50714628710801 17.49773351417477 8.564560489005785 41.43055970971144 2 15.910756502664642 17.454376586376604 52.751394345948235 13.883472565010521 3 19.86175069401067 20.91317678995868 37.68693388508269 21.538138630947966 4 20.296238572193047 25.72882506410406 35.19568571594767 18.779250647755223 5 21.25225272854374 28.507452768876846 36.066295974556645 14.173998528022766 6 17.620697612070796 32.67624077570344 36.50439622798166 13.198665384244107 7 12.961720384321096 20.00750530772488 53.50424518968189 13.52652911827214 8 12.3191383986609 23.722100719106397 47.71240316221368 16.246357720019024 9 15.172183722818172 21.255557116233174 47.17398136691757 16.39827779403108 10-11 17.019642621241303 31.244187404528578 37.653760526234585 14.082409447995534 12-13 16.082536422580475 25.745589971980337 41.87390382569219 16.297969779747003 14-15 16.03842256221434 27.95479780221554 40.01558116976713 15.991198465802983 16-17 17.94743037164171 27.623773625816987 37.993248731895086 16.435547270646214 18-19 17.895993593479588 27.21859323627586 38.5726636393451 16.312749530899453 20-21 18.649487412729492 27.770818569614324 37.08187695445988 16.497817063196297 22-23 17.993426594393007 30.800289385867767 35.561378933430085 15.644905086309144 24-25 17.349992995367227 29.43590284765317 35.20903111476267 18.005073042216928 26-27 17.64407795281042 29.63006064651764 34.23248492852787 18.493376472144075 28-29 18.663390671361658 30.92622860740087 33.01155166237108 17.398829058866394 30-31 18.4133971060837 30.23734844077436 33.55272922367707 17.796525229464866 32-33 17.901079828812126 31.001252726473087 32.28884070955989 18.808826735154895 34-35 19.070596347132376 31.27689686670603 31.803506759783946 17.84900002637765 36-37 18.056760697269993 31.089979478745033 33.03989290470065 17.81336691928432 38-39 19.063742907785887 31.054194833478643 32.23532446668862 17.64673779204686 40-41 18.415715457302824 31.309670861009685 33.33054077650735 16.944072905180143 42-43 18.14161912797822 30.30385406490965 33.632520801343574 17.92200600576855 44-45 18.59440921186499 29.557884866174376 32.97015391991076 18.87755200204987 46-47 17.95246237962361 31.16989215509393 33.464747139444256 17.412898325838196 48-49 18.048393500256566 30.730423256530898 33.74835057696703 17.47283266624551 50-51 17.85084992900691 30.402385841611206 32.55331158862683 19.19345264075505 52-53 19.212894769919487 30.4075308078447 32.4343796107525 17.94519481148331 54-55 17.95694003317848 30.90392531525988 32.265137648286164 18.87399700327548 56-57 18.506419569569136 30.955123505315274 32.71255261146283 17.825904313652753 58-59 18.5420416483612 31.654903768987126 32.03275298099747 17.770301601654204 60-61 18.764824416656964 31.656339925048204 30.99778684923192 18.58104880906291 62-63 18.753615835813722 32.01038495163733 32.009342675984165 17.22665653656479 64-65 18.54344061027038 33.032207510462435 30.571521381390383 17.852830497876802 66-67 18.334305441342195 34.09799803354712 30.134703682386395 17.432992842724293 68-69 18.498730829889315 33.82142195358604 30.369387752791475 17.31045946373317 70-71 18.14463359507049 33.401061950730046 30.279416021919044 18.17488843228042 72-73 18.400535336391215 33.28794070236104 30.141241962311323 18.17028199893642 74-75 18.73611827467968 32.472644938418824 30.49561543372687 18.295621353174624 76-77 18.11374084396433 32.9744470197404 30.65328519111737 18.2585269451779 78-79 18.1854081387767 33.592010677041124 30.127607608606034 18.09497357557614 80-81 18.46097649269782 33.416051395282025 30.218676707490122 17.904295404530032 82-83 18.607888842564545 33.23059387582518 30.371034009781628 17.79048327182864 84-85 18.469300469480558 33.39299680967318 30.095060007971192 18.042642712875075 86-87 18.391102822032096 33.762592086392814 30.034023745629845 17.812281345945244 88-89 18.4199211362663 33.47231827701161 30.0213818055518 18.08637878117029 90-91 17.965765084034885 34.01615738142173 29.719504148971353 18.29857338557204 92-93 18.764019082462667 33.30161300798011 30.030061977284017 17.904305932273207 94-95 18.737224795898594 33.7349116189439 29.35436082436261 18.173502760794904 96-97 18.3669441744388 34.1471075139592 29.71656021033014 17.769388101271858 98-99 19.035129806532698 33.4466162529708 29.36977899988225 18.148474940614257 100-101 19.070251283648414 34.35484117543157 28.628841762964957 17.946065777955063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29493.0 1 27352.0 2 23920.5 3 27163.0 4 37696.5 5 52545.5 6 68389.0 7 84701.5 8 103442.5 9 123284.0 10 143750.0 11 163767.0 12 183855.0 13 203544.5 14 226256.0 15 249344.5 16 268952.0 17 291326.0 18 321932.0 19 355909.5 20 399814.0 21 464405.5 22 545079.0 23 627417.5 24 732989.0 25 881604.0 26 1018896.5 27 1163832.5 28 1313967.5 29 1476614.0 30 1657819.5 31 1762383.5 32 1844181.0 33 1969914.5 34 2086474.5 35 2120940.0 36 2221182.5 37 2359314.5 38 2377008.5 39 2296033.0 40 2291955.5 41 2289047.5 42 1988108.0 43 1671721.5 44 1519808.0 45 1415734.0 46 1344069.0 47 1253217.0 48 1152107.5 49 1052833.5 50 907051.0 51 739171.0 52 603417.0 53 497222.0 54 402376.0 55 325512.5 56 270748.0 57 226958.0 58 189449.0 59 155912.0 60 117081.5 61 82168.5 62 61817.5 63 48125.5 64 35204.5 65 23908.5 66 15961.5 67 11255.5 68 7727.0 69 5228.0 70 3918.0 71 3469.0 72 3153.0 73 2796.0 74 2190.0 75 1463.0 76 1175.0 77 999.0 78 776.5 79 540.5 80 402.5 81 288.0 82 195.5 83 133.0 84 105.5 85 76.5 86 50.5 87 39.0 88 19.5 89 10.0 90 10.5 91 10.0 92 9.5 93 4.5 94 1.5 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1815348020890224 2 4.8473660619945304E-4 3 3.9042987347582403E-4 4 0.0 5 1.8861346544725798E-6 6 1.4711850304886123E-4 7 9.4306732723629E-6 8 3.4327650711400953E-4 9 3.828853348579337E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.82920198170887E-6 26-27 4.432416438010562E-5 28-29 0.00132689572942146 30-31 3.583655843497902E-5 32-33 2.3859603379078136E-4 34-35 2.0841787931922005E-4 36-37 5.356622418702127E-4 38-39 8.39329921240298E-5 40-41 0.004815301772868496 42-43 0.007770874776427028 44-45 0.008336715172768802 46-47 0.005656517828763267 48-49 0.0056131367317103975 50-51 0.0038778928495956243 52-53 0.009120404121702159 54-55 0.008679991679882811 56-57 0.02213756243954467 58-59 0.017932425227398054 60-61 0.0118449256300878 62-63 0.017870182783800457 64-65 0.015980275860018933 66-67 0.025274204369932572 68-69 0.04380359121547119 70-71 0.044611799914912695 72-73 0.05187624753661384 74-75 0.04465518101196556 76-77 0.040248227391790375 78-79 0.0417844840678583 80-81 0.036549517334369654 82-83 0.049389378994691736 84-85 0.06165774185470863 86-87 0.0503032112347837 88-89 0.03624773578965403 90-91 0.037923566430152926 92-93 0.04143837835876258 94-95 0.025387372449200925 96-97 0.018519013104939027 98-99 0.015628511746959797 100-101 0.0036260938732235347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.3018484E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.72325074264215 #Duplication Level Percentage of deduplicated Percentage of total 1 81.07221715220095 24.908020558290033 2 8.130488921002494 4.995900995604672 3 3.021477807298814 2.784888608609102 4 1.6968344316154742 2.0852907884508354 5 1.0699692328189756 1.643646651340492 6 0.7314768729446648 1.3484008427953638 7 0.5428077816810375 1.167377370715071 8 0.42065906052015056 1.033921103481989 9 0.33187982851564074 0.9176784469119998 >10 2.4103099566465582 14.65869046080651 >50 0.30560784872256674 6.568989076053369 >100 0.2269261708239133 13.768016718469081 >500 0.022255906375085673 4.691717040494454 >1k 0.014673795604989414 8.793656656495285 >5k 0.0015721204971057554 3.3492955169710887 >10k+ 8.431127317046782E-4 7.284509164510812 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC 520265 0.9812898460091767 TruSeq Adapter, Index 9 (100% over 50bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG 378209 0.7133531015334199 TruSeq Adapter, Index 9 (100% over 49bp) CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 104385 0.19688416590712024 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 92675 0.17479752910324634 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 83592 0.1576657680366719 No Hit GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 74089 0.13974183041521895 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 72409 0.13657312419570503 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 66032 0.12454524350413339 No Hit ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC 62284 0.11747601081917017 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55273 0.10425232075666291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.8843236634227414 0.0 0.0 1.88613465447258E-5 0.0 2 0.889131420656992 0.0 0.0 2.6405885162616116E-5 0.0 3 0.8892483610055693 0.0 0.0 3.583655843497902E-5 0.0 4 0.8988035191651274 0.0 0.0 3.583655843497902E-5 0.0 5 0.9000238482865711 0.0 0.0 5.281177032523223E-5 0.0 6 0.9009763462870798 0.0 0.0 5.8470174288649974E-5 0.0 7 0.9041846613343376 0.0 1.8861346544725798E-6 7.355925152443062E-5 0.0 8 0.9383727380813076 0.0 1.8861346544725798E-6 7.54453861789032E-5 0.0 9 0.9389725289014299 0.0 1.8861346544725798E-6 9.053446341468384E-5 0.0 10-11 0.9510607659019447 0.0 1.8861346544725798E-6 9.33636653963927E-5 0.0 12-13 0.9590617491062174 0.0 1.8861346544725798E-6 9.902206935981044E-5 0.0 14-15 0.9634677596590653 0.0 1.8861346544725798E-6 1.0750967530493704E-4 0.0 16-17 0.9659499128643512 0.0 1.8861346544725798E-6 1.225987525407177E-4 0.0 18-19 0.9680727574179602 0.0 1.8861346544725798E-6 1.386308971037346E-4 0.0 20-21 1.018584386532063 0.0 1.8861346544725798E-6 1.6315064761187817E-4 0.0 22-23 1.0701183006288901 9.430673272362899E-7 1.8861346544725798E-6 1.782397248476588E-4 0.0 24-25 1.0727466292698975 2.8292019817088694E-6 1.8861346544725798E-6 2.1973468724605555E-4 0.0 26-27 1.0757946228715254 3.7722693089451595E-6 1.8861346544725798E-6 3.743977289128071E-4 0.0 28-29 1.079061408093072 3.7722693089451595E-6 1.8861346544725798E-6 4.8379353887221675E-4 0.0 30-31 1.0829440162793036 4.71533663618145E-6 1.8861346544725798E-6 4.932242121445797E-4 0.0 32-33 1.0871236906736148 5.65840396341774E-6 1.8861346544725798E-6 5.054840873986513E-4 0.0 34-35 1.0912005707292574 5.65840396341774E-6 1.8861346544725798E-6 5.224592992889046E-4 0.0 36-37 1.0961893968903373 7.544538617890319E-6 1.8861346544725798E-6 5.743280022869005E-4 0.0 38-39 1.1014073884119358 7.544538617890319E-6 1.8861346544725798E-6 5.818725409047908E-4 0.0 40-41 1.1079447311243378 7.544538617890319E-6 1.8861346544725798E-6 6.092214933946432E-4 0.0 42-43 1.1217484075930952 7.544538617890319E-6 1.8861346544725798E-6 6.393996478662046E-4 0.0 44-45 1.1275973111566149 9.4306732723629E-6 1.8861346544725798E-6 6.639193983743481E-4 0.0 46-47 1.134853271172371 9.4306732723629E-6 1.8861346544725798E-6 7.346494479170698E-4 1.8861346544725798E-6 48-49 1.1441594595575384 9.4306732723629E-6 1.8861346544725798E-6 7.657706697158674E-4 1.8861346544725798E-6 50-51 1.1589835348743658 9.4306732723629E-6 1.8861346544725798E-6 7.704860063520488E-4 1.8861346544725798E-6 52-53 1.1943155522892732 9.4306732723629E-6 1.8861346544725798E-6 7.761444103154666E-4 1.8861346544725798E-6 54-55 1.208499284890907 9.4306732723629E-6 1.8861346544725798E-6 7.855750835878295E-4 1.8861346544725798E-6 56-57 1.220400794560629 9.4306732723629E-6 1.8861346544725798E-6 7.978349588419013E-4 1.8861346544725798E-6 58-59 1.234325183647273 1.131680792683548E-5 1.8861346544725798E-6 8.10094834095973E-4 1.8861346544725798E-6 60-61 1.2476271482979406 1.3202942581308058E-5 2.8292019817088694E-6 8.39329921240298E-4 1.8861346544725798E-6 62-63 1.265851924396782 1.5089077235780638E-5 3.7722693089451595E-6 8.959139608744754E-4 1.8861346544725798E-6 64-65 1.29082057495269 1.5089077235780638E-5 3.7722693089451595E-6 9.713593470533786E-4 1.8861346544725798E-6 66-67 1.3128336525050397 1.6032144563016927E-5 3.7722693089451595E-6 0.0010317156559965011 1.8861346544725798E-6 68-69 1.335157942275377 1.6975211890253217E-5 3.7722693089451595E-6 0.00106000767581359 1.8861346544725798E-6 70-71 1.3605905819562853 1.88613465447258E-5 3.7722693089451595E-6 0.0010703814164131891 1.8861346544725798E-6 72-73 1.3967911643795774 1.9804413871962088E-5 3.7722693089451595E-6 0.0010779259550310794 1.8861346544725798E-6 74-75 1.4331134024880834 2.263361585367096E-5 3.7722693089451595E-6 0.0011213070520839487 1.8861346544725798E-6 76-77 1.472353490907058 2.263361585367096E-5 3.7722693089451595E-6 0.0013683906918198568 1.8861346544725798E-6 78-79 1.5171086370557105 2.4519750508143537E-5 3.7722693089451595E-6 0.0014711850304886124 3.7722693089451595E-6 80-81 1.5672534129795186 2.4519750508143537E-5 3.7722693089451595E-6 0.0016003852543199839 3.7722693089451595E-6 82-83 1.6340678469795553 2.4519750508143537E-5 3.7722693089451595E-6 0.0016796029098078325 3.7722693089451595E-6 84-85 1.7091991917384888 2.5462817835379826E-5 3.7722693089451595E-6 0.0017003503910070308 3.7722693089451595E-6 86-87 1.79591611861252 2.6405885162616116E-5 3.7722693089451595E-6 0.0017116671989338662 3.7722693089451595E-6 88-89 1.8998534548818862 2.8292019817088698E-5 4.71533663618145E-6 0.0017267562761696467 3.7722693089451595E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 48870 0.0 70.99272 1 GCGTGTT 62860 0.0 55.751217 2 CGGTGGT 6145 0.0 45.21687 1 GTTTTTT 880785 0.0 39.556942 1 CCCGAAA 8315 0.0 36.96407 1 CGGCCTT 5720 0.0 34.519268 1 CGGAAGA 204175 0.0 34.38197 4 CGTGTTT 105665 0.0 33.692055 3 TCGGAAG 200525 0.0 33.530125 3 ATCGGAA 204585 0.0 33.347607 2 GATCGGA 205405 0.0 32.95428 1 CCGAGAT 8010 0.0 32.1347 1 GTGTTTT 260690 0.0 31.805906 1 GAGCACA 223385 0.0 31.688883 9 AGATCGG 145520 0.0 31.557613 1 AGAGCAC 230820 0.0 30.777184 8 CTCGTAT 179230 0.0 30.103613 42-43 CCGAAAC 10140 0.0 29.73775 2 GAAGAGC 242735 0.0 29.323175 6 ACGATCA 206480 0.0 28.999765 32-33 >>END_MODULE