FastQCFastQC Report
Fri 27 May 2016
SRR1233043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50163674
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1413670.2818114957050395No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1128580.22497953399505788No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1120250.22331896981867796No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC795990.1586785688783481No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC772650.1540257996254421No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT750190.14954845611986076No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT718770.14328495955061027No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA662850.13213745069788949No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC595680.11874728314357516No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT564020.11243594318869068No Hit
CTACATTTTCTTATAAAAGAACATTACTATACCCTTTATGAAACTAAAGG555460.1107295291010782No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA549470.10953543793462975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT386450.061.7054751
CCCGAAA171550.051.447971
GCGTGTT508550.046.786842
GTTTTTT7167150.045.969481
CGGGCTA25650.038.5202671
CCGAAAC242150.036.2709352
CGCCGTA111100.036.17534346-47
GTCGCCG124500.034.4345144-45
CGTGTTT726900.033.0921823
TCGTCTA228150.032.67041844-45
CGAGCTA232050.032.40032212-13
ACTCGTC230650.032.17024642-43
CGAAACC275300.031.9379163
CCCAGAT340050.031.4865041
CTAACGA224800.031.2929294-95
CCGTATC130450.031.2289448-49
TCGGTGG155150.030.67067138-39
CGGCCTT62600.029.8216381
GATCTCG153200.029.0379734-35
CAAAACG570050.028.9761689
Produced by FastQC (version 0.11.5)