##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233043_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50163674 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.753622511780137 33.0 31.0 34.0 30.0 34.0 2 32.02386685233621 33.0 31.0 34.0 30.0 34.0 3 32.05771002737957 34.0 31.0 34.0 30.0 34.0 4 35.480913180322474 37.0 35.0 37.0 33.0 37.0 5 35.43198654069875 37.0 35.0 37.0 33.0 37.0 6 35.43875257222986 37.0 35.0 37.0 33.0 37.0 7 35.53337006376367 37.0 35.0 37.0 33.0 37.0 8 35.537640345083176 37.0 35.0 37.0 33.0 37.0 9 37.12427566609256 39.0 37.0 39.0 33.0 39.0 10-11 37.02493490608363 39.0 37.0 39.0 33.0 39.0 12-13 36.982842385109194 39.0 37.0 39.0 33.0 39.0 14-15 38.226741057682496 40.0 38.0 41.0 33.0 41.0 16-17 37.9557333161044 40.0 38.0 41.0 33.0 41.0 18-19 37.54045375544064 40.0 37.0 41.0 32.0 41.0 20-21 37.255094064681145 40.0 37.0 41.0 32.0 41.0 22-23 36.981686897574534 40.0 37.0 41.0 31.0 41.0 24-25 36.70919558244478 40.0 37.0 41.0 30.5 41.0 26-27 36.293685197380086 40.0 36.0 41.0 28.0 41.0 28-29 35.92965335832459 40.0 36.0 41.0 27.0 41.0 30-31 35.66557114616445 40.0 36.0 41.0 25.5 41.0 32-33 35.459872915209516 40.0 36.0 41.0 24.0 41.0 34-35 35.27251737980755 40.0 36.0 41.0 23.5 41.0 36-37 35.148532760977595 40.0 36.0 41.0 23.0 41.0 38-39 35.00068409064296 40.0 35.5 41.0 20.5 41.0 40-41 34.92668204485979 40.0 35.0 41.0 19.5 41.0 42-43 34.987134983374624 40.0 35.5 41.0 20.0 41.0 44-45 34.901573008787196 40.0 35.0 41.0 19.0 41.0 46-47 34.78833750295084 40.0 35.0 41.0 18.0 41.0 48-49 34.66317945531661 40.0 35.0 41.0 17.5 41.0 50-51 34.23228519904663 38.5 34.5 40.5 15.0 41.0 52-53 34.249363064994 39.0 35.0 40.0 14.5 41.0 54-55 34.402961732826824 39.0 35.0 41.0 13.5 41.0 56-57 34.29281334138325 39.0 35.0 41.0 10.5 41.0 58-59 34.12557410567655 39.0 34.5 41.0 8.5 41.0 60-61 33.95085059957929 39.0 34.0 41.0 8.0 41.0 62-63 33.694871920665136 38.5 34.0 40.5 7.0 41.0 64-65 33.4355144421838 38.0 34.0 40.0 6.0 41.0 66-67 33.168635674492265 38.0 34.0 40.0 2.0 41.0 68-69 32.88731350698117 37.5 33.0 40.0 2.0 41.0 70-71 32.52586174210445 37.0 33.0 39.5 2.0 41.0 72-73 32.17765088338625 36.0 32.0 39.0 2.0 41.0 74-75 31.79939789697222 36.0 32.0 39.0 2.0 40.5 76-77 31.395273958601997 35.5 31.5 38.5 2.0 40.0 78-79 30.985363791336333 35.0 31.0 37.5 2.0 39.5 80-81 30.571362436491395 35.0 31.0 37.0 2.0 39.0 82-83 30.247777295977166 35.0 31.0 36.5 2.0 39.0 84-85 29.86355098312775 35.0 30.5 36.0 2.0 37.5 86-87 29.48808993735188 35.0 30.0 36.0 2.0 37.0 88-89 29.172581348407615 34.0 30.0 35.0 2.0 37.0 90-91 28.88526748459453 34.0 30.0 35.0 2.0 36.0 92-93 28.499155753623626 34.0 29.0 35.0 2.0 36.0 94-95 28.135241788709497 34.0 29.0 35.0 2.0 36.0 96-97 27.725958788425267 34.0 29.0 35.0 2.0 35.5 98-99 27.258020225950755 34.0 28.0 35.0 2.0 35.0 100-101 24.758983841574285 31.5 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3627683770256098 1101 2 0.2993293769645291 1101 3 0.29684819068511814 1101 4 0.328443818665086 1101 5 0.33894553664192273 1101 6 0.34722388272439275 1101 7 0.31433610595890826 1101 8 0.2881056717210484 1101 9 0.4224628580599159 1101 10-11 0.4776339796131239 1101 12-13 0.43631831592215775 1101 14-15 0.5039910432632766 1101 16-17 0.5013855310126161 1101 18-19 0.5207865696378278 1101 20-21 0.47599426266475575 1101 22-23 0.4900884355265518 1101 24-25 0.46545720401690005 1101 26-27 0.4786120574054067 1101 28-29 0.39778333701169544 1101 30-31 0.4231298346841541 1101 32-33 0.3899885054894483 1101 34-35 0.4182120742499009 1101 36-37 0.3988068466961252 1101 38-39 0.3986272991478188 1101 40-41 0.44997664584008135 1101 42-43 0.4316721183881782 1101 44-45 0.44726444212462724 1101 46-47 0.3895497908520795 1101 48-49 0.4004843554161184 1101 50-51 0.41540377517201676 1101 52-53 0.4497354697818068 1101 54-55 0.46985070470886825 1101 56-57 0.4460422175552594 1101 58-59 0.4589607969798877 1101 60-61 0.44603138401579656 1101 62-63 0.4215586582139892 1101 64-65 0.4107199993750683 1101 66-67 0.3700344491246952 1101 68-69 0.373718027425781 1101 70-71 0.29281223228976216 1101 72-73 0.3081649227803638 1101 74-75 0.29775948345602643 1101 76-77 0.2602564119655888 1101 78-79 0.3640816521080872 1101 80-81 0.31621543943950314 1101 82-83 0.30640252322930905 1101 84-85 0.28553882421963195 1101 86-87 0.28554719455392785 1101 88-89 0.1985305154034407 1101 90-91 0.16718143038944433 1101 92-93 0.07864788322578065 1101 94-95 0.17434926560478559 1101 96-97 0.10581420359298832 1101 98-99 0.1308032683590845 1101 100-101 0.3695339760505014 1102 1 0.28647125529092676 1102 2 0.25792536201812766 1102 3 0.2733294000495974 1102 4 0.25624046872503925 1102 5 0.2511474791193038 1102 6 0.2915231465648347 1102 7 0.23852746654699075 1102 8 0.22265903665829256 1102 9 0.35770787752776556 1102 10-11 0.34918130259146807 1102 12-13 0.35852356674808306 1102 14-15 0.406013330806978 1102 16-17 0.3860675872552477 1102 18-19 0.41359420200458885 1102 20-21 0.3501093346574251 1102 22-23 0.363883241115623 1102 24-25 0.3692571905496891 1102 26-27 0.34411625448778693 1102 28-29 0.2987775810385571 1102 30-31 0.3320213862740431 1102 32-33 0.3270769628123844 1102 34-35 0.3278966509656982 1102 36-37 0.3566339442133426 1102 38-39 0.35238830545491595 1102 40-41 0.42419918299518855 1102 42-43 0.3416536462207347 1102 44-45 0.3023346880538753 1102 46-47 0.24236354878094346 1102 48-49 0.2823559048729649 1102 50-51 0.2971166374774228 1102 52-53 0.29697632040598165 1102 54-55 0.34685220534364447 1102 56-57 0.32472299193764087 1102 58-59 0.3150480436893943 1102 60-61 0.2690580692458866 1102 62-63 0.26301811337699377 1102 64-65 0.2718542407971043 1102 66-67 0.3174924198992741 1102 68-69 0.3669815364515472 1102 70-71 0.33260046116316033 1102 72-73 0.2715513990278353 1102 74-75 0.2519051252216613 1102 76-77 0.22475237547457283 1102 78-79 0.26097298165752747 1102 80-81 0.26329149440776334 1102 82-83 0.20839520600438632 1102 84-85 0.19684970387069356 1102 86-87 0.20153200310998898 1102 88-89 0.12541899050375704 1102 90-91 0.09255838814536688 1102 92-93 0.08056264964969628 1102 94-95 0.11412534237145167 1102 96-97 0.1296011674557498 1102 98-99 -0.08107340400393426 1102 100-101 0.1230631237890023 1103 1 0.16942306265435647 1103 2 0.20607152065655043 1103 3 0.2659000766083892 1103 4 0.2804610773106688 1103 5 0.2461556974064294 1103 6 0.25213743231682173 1103 7 0.2517680537815181 1103 8 0.24053981272768254 1103 9 0.299454669026062 1103 10-11 0.35409121201674765 1103 12-13 0.32748174127944907 1103 14-15 0.3751882724304423 1103 16-17 0.3767279246465378 1103 18-19 0.3586567257424136 1103 20-21 0.35991241243996086 1103 22-23 0.3939331456162236 1103 24-25 0.3795348015889317 1103 26-27 0.3972185771866421 1103 28-29 0.44016108506772156 1103 30-31 0.4261711838161233 1103 32-33 0.33894065962265074 1103 34-35 0.35818245491757494 1103 36-37 0.3888781953200251 1103 38-39 0.2668240043868977 1103 40-41 0.3645970853172358 1103 42-43 0.3324260657425242 1103 44-45 0.3566117903855073 1103 46-47 0.3702299892831675 1103 48-49 0.41339432407117016 1103 50-51 0.4086943374897487 1103 52-53 0.44660114549967744 1103 54-55 0.436102026047557 1103 56-57 0.43757441582160794 1103 58-59 0.4648665308871571 1103 60-61 0.45025608581399723 1103 62-63 0.3737885333896216 1103 64-65 0.3524363780957813 1103 66-67 0.3756401964146434 1103 68-69 0.36286647758985424 1103 70-71 0.38827746958890685 1103 72-73 0.3399635126835818 1103 74-75 0.4026755878634276 1103 76-77 0.3535447856220095 1103 78-79 0.39962849343138984 1103 80-81 0.39891344832810915 1103 82-83 0.37861860294105654 1103 84-85 0.36108386851941177 1103 86-87 0.3360940385412903 1103 88-89 0.26187240122994027 1103 90-91 0.27811762243688776 1103 92-93 0.30424467166703195 1103 94-95 0.34308714037880605 1103 96-97 0.31515813834350936 1103 98-99 0.2130093967866955 1103 100-101 0.08552688852168089 1104 1 0.27270796696505784 1104 2 0.2147162035455139 1104 3 0.2291788239086472 1104 4 0.280466602645447 1104 5 0.24249708807094095 1104 6 0.26390881960274726 1104 7 0.2654906582046479 1104 8 0.2871193825978864 1104 9 0.3567267041673361 1104 10-11 0.3761030059406707 1104 12-13 0.37051270194286445 1104 14-15 0.418339839250244 1104 16-17 0.35468306256650095 1104 18-19 0.393860547594592 1104 20-21 0.43128093752532237 1104 22-23 0.47966503874149424 1104 24-25 0.4667062066467196 1104 26-27 0.48800491638093746 1104 28-29 0.4809402535493845 1104 30-31 0.4571045149447954 1104 32-33 0.45118123318610515 1104 34-35 0.46453702237058536 1104 36-37 0.4387277094347084 1104 38-39 0.4113052255395644 1104 40-41 0.5194123059525211 1104 42-43 0.3886430506541103 1104 44-45 0.40755300763320434 1104 46-47 0.37295428956216625 1104 48-49 0.4612716713116143 1104 50-51 0.4518878874331165 1104 52-53 0.48460763220291625 1104 54-55 0.5325684966963351 1104 56-57 0.47482709105393184 1104 58-59 0.5103861947950961 1104 60-61 0.5261976531917654 1104 62-63 0.5106236054681617 1104 64-65 0.48650640385061905 1104 66-67 0.4623947576613361 1104 68-69 0.4544499185038404 1104 70-71 0.4241832205179037 1104 72-73 0.4424070110799718 1104 74-75 0.4492851454760114 1104 76-77 0.34329333846609345 1104 78-79 0.4711209706537751 1104 80-81 0.4364924665135206 1104 82-83 0.43119241510138195 1104 84-85 0.4231145934657903 1104 86-87 0.4481375354693462 1104 88-89 0.3625785623225575 1104 90-91 0.4149233254139979 1104 92-93 0.3905785769586707 1104 94-95 0.42214950897470516 1104 96-97 0.4082870795934923 1104 98-99 0.32183953044956937 1104 100-101 0.16789164661351563 1105 1 0.15317986876285516 1105 2 0.09617930500498062 1105 3 0.1366830576041096 1105 4 0.17430999872656372 1105 5 0.18648311927002226 1105 6 0.2200378559592977 1105 7 0.1800989026420936 1105 8 0.19357991882968406 1105 9 0.19919018308434033 1105 10-11 0.24156296753340456 1105 12-13 0.2076445177505022 1105 14-15 0.29977998930205985 1105 16-17 0.29623647225497507 1105 18-19 0.24822509590768504 1105 20-21 0.24882601853185093 1105 22-23 0.2930386451757414 1105 24-25 0.2587688568937381 1105 26-27 0.2989348576765636 1105 28-29 0.24260301268740392 1105 30-31 0.2758545962431356 1105 32-33 0.25775161648594036 1105 34-35 0.228686527924566 1105 36-37 0.20563704654973236 1105 38-39 0.19227748810582312 1105 40-41 0.28317526080684274 1105 42-43 0.21918952041272632 1105 44-45 0.2057361469039236 1105 46-47 0.17831013191761969 1105 48-49 0.25067385102489226 1105 50-51 0.25945567580156137 1105 52-53 0.23955390178907976 1105 54-55 0.29541608751592463 1105 56-57 0.2978373149653919 1105 58-59 0.3350341411276716 1105 60-61 0.26010672159162596 1105 62-63 0.21101147769515194 1105 64-65 0.24869350178948935 1105 66-67 0.23788338720631685 1105 68-69 0.28293941751933005 1105 70-71 0.1934893130971247 1105 72-73 0.20590860712072612 1105 74-75 0.25730275577852524 1105 76-77 0.23651576968373433 1105 78-79 0.28333346767211154 1105 80-81 0.27994636020061137 1105 82-83 0.26519002523995994 1105 84-85 0.25972900048604686 1105 86-87 0.2602507995414065 1105 88-89 0.2140278282446566 1105 90-91 0.218405178160463 1105 92-93 0.21687513002974512 1105 94-95 0.14171768942128793 1105 96-97 0.1396493511458452 1105 98-99 0.09083981179890444 1105 100-101 0.0756796046781183 1106 1 0.0981125054701053 1106 2 0.0475182824171938 1106 3 0.03862547580033038 1106 4 0.08891516405872579 1106 5 0.11767464476328371 1106 6 0.06750414018185324 1106 7 0.0980804229509431 1106 8 0.09839222077665255 1106 9 0.11622639455964645 1106 10-11 0.09647251786882549 1106 12-13 0.14056961245230326 1106 14-15 0.11088217784112686 1106 16-17 0.09388882045399072 1106 18-19 0.10745682985282201 1106 20-21 0.1391961384957554 1106 22-23 0.14798104116997735 1106 24-25 0.10778225374772887 1106 26-27 0.12194697520829578 1106 28-29 0.1675289669029567 1106 30-31 0.13700818394482184 1106 32-33 0.1245829723094829 1106 34-35 0.15902788374683752 1106 36-37 0.10504994876945517 1106 38-39 0.14065115071877443 1106 40-41 0.22423330827787424 1106 42-43 0.1365999017372701 1106 44-45 0.08415863497253895 1106 46-47 0.12754147397085802 1106 48-49 0.14187463950572266 1106 50-51 0.12457450692940597 1106 52-53 0.18242589547446642 1106 54-55 0.17090254077242406 1106 56-57 0.2267866186194496 1106 58-59 0.22645867746371806 1106 60-61 0.22621839439207747 1106 62-63 0.2431368370795326 1106 64-65 0.19560492885663194 1106 66-67 0.13570128679152305 1106 68-69 0.1556432659686422 1106 70-71 0.19351396878973048 1106 72-73 0.2084268997549259 1106 74-75 0.1797456669282056 1106 76-77 0.1727404932624914 1106 78-79 0.16996703379728828 1106 80-81 0.16747225141952526 1106 82-83 0.09463580773449465 1106 84-85 0.18748713704178854 1106 86-87 0.24284564586677604 1106 88-89 0.15330550049213443 1106 90-91 0.2037560879983431 1106 92-93 0.1335342011331484 1106 94-95 0.1392168871917896 1106 96-97 0.04953781194524609 1106 98-99 0.03559669006309818 1106 100-101 0.11469803853291793 1107 1 0.010010344531625748 1107 2 -0.021242326744172146 1107 3 -0.010803179806195828 1107 4 0.03640127945325844 1107 5 0.025262559388288253 1107 6 0.08363854200415943 1107 7 0.0566450978722699 1107 8 0.04403511857167075 1107 9 0.08699869000771798 1107 10-11 0.12329570714192783 1107 12-13 0.0941721749611446 1107 14-15 0.05504513790537402 1107 16-17 0.02555556697470962 1107 18-19 0.010991447726432568 1107 20-21 -0.041686655301511166 1107 22-23 0.04143616343736056 1107 24-25 -0.02647007072756935 1107 26-27 0.024316907102445384 1107 28-29 -0.002808573195927977 1107 30-31 -0.034243169250025574 1107 32-33 -0.031417625034436014 1107 34-35 0.00914121369196863 1107 36-37 -0.006790844480434544 1107 38-39 0.03434100062865042 1107 40-41 0.07609053263519172 1107 42-43 0.023906858924419794 1107 44-45 0.03977122690258028 1107 46-47 0.03380269978612205 1107 48-49 0.016114988266700436 1107 50-51 0.003504883561319616 1107 52-53 0.04051020356862267 1107 54-55 0.053401782554182375 1107 56-57 0.08554383880176175 1107 58-59 0.015615585952112099 1107 60-61 0.020231341194275387 1107 62-63 0.06860961062246673 1107 64-65 0.019190645014127483 1107 66-67 0.05637136747777305 1107 68-69 0.08852999073211976 1107 70-71 0.03503555901833266 1107 72-73 0.021666607512024427 1107 74-75 -0.11433389994378729 1107 76-77 -0.026901920879847552 1107 78-79 0.06262705930755885 1107 80-81 0.04006188577002945 1107 82-83 -0.05473577532981011 1107 84-85 -0.008191186331156786 1107 86-87 -0.04771649302135117 1107 88-89 -0.07625236936110369 1107 90-91 -0.06262128321902694 1107 92-93 -0.11980733022093304 1107 94-95 -0.036954845639705525 1107 96-97 -0.1157399262097023 1107 98-99 -0.16001564737303653 1107 100-101 -0.16396658378534212 1108 1 -0.10969805272304356 1108 2 -0.12308842723123092 1108 3 -0.17740051862870487 1108 4 -0.10209946491168154 1108 5 -0.09189305498566114 1108 6 -0.07445636052575111 1108 7 -0.07213924236764768 1108 8 -0.05272136205321942 1108 9 -0.041943101371067826 1108 10-11 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-0.521717614187434 2316 74-75 -0.48506396868929613 2316 76-77 -0.6167015853447886 2316 78-79 -0.5255598565574893 2316 80-81 -0.5243013728719284 2316 82-83 -0.3862684848803468 2316 84-85 -0.5211319628788225 2316 86-87 -0.5347979868795356 2316 88-89 -0.4741177774502212 2316 90-91 -0.5546071908616312 2316 92-93 -0.6567043049474357 2316 94-95 -0.638612825315434 2316 96-97 -0.5733449103651829 2316 98-99 -0.5418561370804476 2316 100-101 -0.6322039824505516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 188525.0 3 38984.0 4 35612.0 5 50734.0 6 112980.0 7 245532.0 8 436808.0 9 650466.0 10 768528.0 11 660331.0 12 453409.0 13 309673.0 14 239587.0 15 205607.0 16 187624.0 17 183142.0 18 187562.0 19 196832.0 20 212367.0 21 231854.0 22 258733.0 23 290354.0 24 326795.0 25 368058.0 26 425617.0 27 500651.0 28 595423.0 29 717907.0 30 874682.0 31 1083217.0 32 1365873.0 33 1763032.0 34 2354772.0 35 3286695.0 36 4913319.0 37 8841028.0 38 1.2676601E7 39 3922935.0 40 1825.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.593067409467018 16.280959621719443 9.417172902052139 44.7088000667614 2 13.995148366428111 17.221066454334437 54.96247246321375 13.821312716023703 3 18.96093571418632 21.000354498811603 38.417186928527165 21.62152285847491 4 19.773155854134878 27.070107024609296 35.1716685644825 17.985068556773324 5 19.942565592398086 31.19704937000184 35.05542525868266 13.80495977891741 6 15.018167779180548 34.41061473777074 36.354206039359546 14.217011443689175 7 12.43214091473722 18.811801119560513 54.372485970402415 14.383571995299855 8 13.105204375580623 22.39092774584254 46.99685872290774 17.507009155669103 9 14.262063420633824 21.63093357157213 46.55717202850812 17.549830979285925 10-11 16.822097368925743 32.55211761953598 36.063122450551795 14.562662560986487 12-13 16.449699259765644 26.0781551530336 41.51411817888297 15.958027408317784 14-15 17.352631507811807 28.222811192019147 39.088370002564005 15.336187297605036 16-17 18.263191860415397 28.601146595399644 37.10934778438431 16.02631375980065 18-19 18.440450540258325 28.761292180114157 36.12208256452036 16.676174715107155 20-21 18.729748844454996 29.210675930257608 35.00856949011927 17.05100573516812 22-23 18.367129463711915 30.159295459639086 35.06899294683169 16.40458212981731 24-25 18.24167045503424 30.31676301937872 34.18686895394852 17.254697571638523 26-27 17.97658234203075 30.993885782161577 33.613016708345086 17.416515167462588 28-29 18.77563010140087 30.429222765032033 33.085665060564146 17.70948207300295 30-31 18.55474320953336 30.950021621715585 32.94349256532744 17.551742603423605 32-33 18.089345687965174 30.33365236802371 33.26230689890606 18.314695045105054 34-35 18.48872252967087 31.147054419035285 32.27317374745697 18.09104930383687 36-37 18.54378872898075 31.143566631957608 32.38262988056972 17.930014758491915 38-39 18.542008077539112 30.57435471538481 32.70881836062776 18.17481884644831 40-41 18.742105551993788 30.915293318800853 32.38076053454866 17.9618405946567 42-43 18.38256615615972 31.246037251009234 32.658162159239716 17.713234433591328 44-45 18.838066861712363 30.381923950398715 32.353810493476594 18.426198694412328 46-47 18.3834887755459 31.524355152965345 31.683709034841673 18.408447036647086 48-49 18.089127086019857 31.042091736353715 32.37331567239755 18.49546550522888 50-51 18.04475236065076 31.156265397783393 32.80866285991715 17.990319381648696 52-53 18.611626172786387 30.985105629433473 32.106741452356005 18.29652674542413 54-55 18.92688199690933 31.460328196790915 31.293207473136352 18.3195823331634 56-57 19.39452863158532 31.064246060848937 31.384850509276774 18.15637479828897 58-59 18.407775645757283 32.27208575622965 31.375746950666073 17.944391647346997 60-61 18.83532944948058 32.08948537422482 30.654536066581883 18.420649109712713 62-63 18.714764503687885 32.6060667474539 30.555484437689444 18.123684311168766 64-65 18.668223076298133 31.662895430306225 31.104355371781367 18.56452612161427 66-67 18.92577532918077 32.197142439418194 30.62159416358855 18.255488067812482 68-69 19.11250728216033 31.67646050951738 30.88864274908094 18.322389459241354 70-71 18.283537577617917 31.817883536114255 30.829458686430677 19.06912019983715 72-73 18.37704068988559 31.761957988139244 31.006199712846044 18.85480160912912 74-75 18.700386292898003 31.374881133476233 31.160797267641684 18.76393530598408 76-77 18.28183026703245 31.980956218713626 30.83356780807035 18.903645706183582 78-79 18.518404955660646 32.657799742983876 30.41815161598113 18.405643685374347 80-81 18.41003599289163 32.720920402983026 30.75097005142285 18.11807355270249 82-83 19.127410738081707 32.281223944617516 30.354091513574062 18.23727380372672 84-85 18.743253448241447 32.23252420318975 30.555148065478143 18.469074283090666 86-87 18.655933507551822 32.73022919446267 30.60860874913963 18.00522854884587 88-89 18.83895576731588 32.09410988970763 30.631538633630246 18.435395709346242 90-91 18.384978740999653 32.97588559694501 30.13017915095834 18.508956511096986 92-93 19.234647196450464 32.18746089170853 30.33612173208225 18.241770179758756 94-95 19.13203401313256 32.589065759846605 29.74417645132815 18.534723775692683 96-97 18.808486969367326 33.31475876374146 29.925756804426207 17.950997462465008 98-99 19.762992331770445 32.29625880734541 29.451706606251594 18.48904225463255 100-101 19.926956404460576 33.47388376672263 28.29200173536356 18.307158093453236 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15340.0 1 19328.0 2 22790.5 3 26766.0 4 37228.0 5 49865.0 6 62344.0 7 74933.0 8 89044.5 9 103218.0 10 116819.5 11 129713.5 12 142206.5 13 154883.5 14 170496.5 15 185269.0 16 198331.5 17 214707.0 18 236117.5 19 265196.0 20 305678.5 21 359372.0 22 429084.5 23 512546.0 24 616464.5 25 771367.0 26 917989.5 27 1100806.0 28 1245470.0 29 1360912.0 30 1532713.5 31 1648832.5 32 1769946.0 33 1920107.5 34 2076909.5 35 2189726.5 36 2305747.0 37 2366814.0 38 2372780.0 39 2297090.5 40 2246018.5 41 2242361.5 42 1953809.5 43 1633596.0 44 1447233.0 45 1289600.5 46 1170556.5 47 1077852.0 48 1022257.5 49 951514.0 50 818513.5 51 678593.5 52 584942.5 53 509464.0 54 425488.5 55 343987.5 56 284817.5 57 247262.0 58 213329.0 59 172884.5 60 125608.0 61 85257.0 62 61059.0 63 44658.5 64 32213.0 65 22049.5 66 14971.5 67 10743.5 68 7976.0 69 5744.5 70 4497.0 71 4295.0 72 4252.5 73 4612.0 74 3955.5 75 2548.5 76 2094.5 77 1760.5 78 1351.0 79 868.0 80 555.5 81 379.0 82 245.5 83 149.0 84 98.5 85 82.0 86 78.0 87 54.5 88 30.5 89 23.5 90 18.5 91 16.0 92 12.0 93 9.0 94 10.5 95 9.5 96 5.5 97 4.5 98 4.5 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053660343937328034 2 0.0015469361354991662 3 0.005414276474247082 4 0.031247711242202873 5 0.07569620997058549 6 0.01454438923273443 7 0.017215644930632473 8 0.0 9 0.0 10-11 9.967372007082256E-7 12-13 6.67813924474511E-5 14-15 0.0 16-17 6.57846552467429E-5 18-19 6.478791804603466E-5 20-21 0.0028167793292014453 22-23 0.0015080633846715453 24-25 0.009262678806181542 26-27 0.007215380595926846 28-29 3.9869488028329024E-6 30-31 0.0022765477664175872 32-33 0.001784159589267724 34-35 0.00200543524782495 36-37 2.3921692816997416E-5 38-39 0.002230697855185009 40-41 0.19699813055957582 42-43 0.003985952065632194 44-45 0.0017931302240740978 46-47 0.007833357660365945 48-49 0.007986855189275012 50-51 0.018612073748824697 52-53 0.004396607792323984 54-55 0.010610267501539062 56-57 0.008686564704172187 58-59 0.012449247636845738 60-61 0.013168891895757076 62-63 0.02429646600446371 64-65 0.010962115733389064 66-67 0.03560444954649853 68-69 0.025641064488219106 70-71 0.010430854805411581 72-73 0.00622960750442641 74-75 0.02358878259196087 76-77 0.010890350654938074 78-79 0.04012365601450962 80-81 0.0908695403769668 82-83 0.05517937143120737 84-85 0.08604333885113759 86-87 0.03554165510285391 88-89 0.014103831390021391 90-91 0.020588603617829108 92-93 0.02882962679328472 94-95 0.05882543611139806 96-97 0.053874642435480305 98-99 0.06253927892123691 100-101 0.053085226572519385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.0163674E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.026241374461275 #Duplication Level Percentage of deduplicated Percentage of total 1 80.20697630584097 21.676931015573544 2 8.327854062073204 4.501411880257565 3 3.203252940206882 2.597156614364519 4 1.7738318213695845 1.9176002784813868 5 1.1200311374773397 1.5135115934187502 6 0.7774990679942573 1.2607726488018887 7 0.5551925061327387 1.0503336676024828 8 0.4267063455494293 0.9225814952666526 9 0.3449498556966175 0.8390428255928133 >10 2.5970366112935452 14.110591444711151 >50 0.3435405745537802 6.481171640067022 >100 0.267981365581612 14.465638627733343 >500 0.030747882216654608 5.725734940103864 >1k 0.020722300579816732 10.888240309789596 >5k 0.0023251939004890706 4.31156627201338 >10k+ 0.0013520295333115722 7.737714746222204 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 141367 0.2818114957050395 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 112858 0.22497953399505788 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 112025 0.22331896981867796 No Hit CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC 79599 0.1586785688783481 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 77265 0.1540257996254421 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 75019 0.14954845611986076 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 71877 0.14328495955061027 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 66285 0.13213745069788949 No Hit GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 59568 0.11874728314357516 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56402 0.11243594318869068 No Hit CTACATTTTCTTATAAAAGAACATTACTATACCCTTTATGAAACTAAAGG 55546 0.1107295291010782 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 54947 0.10953543793462975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.12226775893647662 0.0 0.0 1.594779521133161E-5 0.0 2 0.12281197744806331 1.9934744014164512E-6 0.0 2.3921692816997416E-5 1.9934744014164512E-6 3 0.12285184693609165 1.9934744014164512E-6 0.0 2.5915167218413868E-5 1.9934744014164512E-6 4 0.12374492346792622 1.9934744014164512E-6 0.0 3.189559042266322E-5 1.9934744014164512E-6 5 0.12395822522887777 1.9934744014164512E-6 0.0 4.385643683116193E-5 1.9934744014164512E-6 6 0.12416953351542792 1.9934744014164512E-6 0.0 5.1830334436827736E-5 1.9934744014164512E-6 7 0.12505662962405822 1.9934744014164512E-6 0.0 8.372592485949096E-5 1.9934744014164512E-6 8 0.1367802525787884 1.9934744014164512E-6 0.0 8.771287366232386E-5 1.9934744014164512E-6 9 0.1369736195957258 1.9934744014164512E-6 0.0 9.768024566940611E-5 1.9934744014164512E-6 10-11 0.1388923387070891 1.9934744014164512E-6 0.0 9.967372007082257E-5 1.9934744014164512E-6 12-13 0.14036551628973587 1.9934744014164512E-6 0.0 1.086443548771966E-4 1.9934744014164512E-6 14-15 0.1415416661865716 1.9934744014164512E-6 0.0 1.2658562448994464E-4 1.9934744014164512E-6 16-17 0.14247760241803661 2.9902116021246766E-6 0.0 1.4652036850410917E-4 1.9934744014164512E-6 18-19 0.14339360390548744 3.9869488028329024E-6 0.0 2.0233765174376982E-4 1.9934744014164512E-6 20-21 0.1447790686144719 3.9869488028329024E-6 0.0 2.631386209869716E-4 1.9934744014164512E-6 22-23 0.14636188728919655 3.9869488028329024E-6 0.0 2.8706031380396904E-4 1.9934744014164512E-6 24-25 0.14768256408013497 3.9869488028329024E-6 0.0 3.2892327623371446E-4 3.9869488028329024E-6 26-27 0.149011214768679 3.9869488028329024E-6 0.0 4.4055784271303575E-4 3.9869488028329024E-6 28-29 0.1503916957916599 3.9869488028329024E-6 0.0 5.013588119562375E-4 3.9869488028329024E-6 30-31 0.15177516702624294 7.973897605665805E-6 0.0 5.52192409192357E-4 3.9869488028329024E-6 32-33 0.1528376888821979 7.973897605665805E-6 0.0 5.591695695973146E-4 3.9869488028329024E-6 34-35 0.15358225157112693 7.973897605665805E-6 0.0 5.900684228192696E-4 5.980423204249354E-6 36-37 0.15398692687461446 9.967372007082256E-6 0.0 6.31931385249015E-4 5.980423204249354E-6 38-39 0.15431485341364748 9.967372007082256E-6 0.0 6.40902020055389E-4 5.980423204249354E-6 40-41 0.15471354829393077 9.967372007082256E-6 0.0 6.638269756716783E-4 5.980423204249354E-6 42-43 0.15549200004768393 9.967372007082256E-6 0.0 7.216377333127554E-4 5.980423204249354E-6 44-45 0.1565216295760155 9.967372007082256E-6 0.0 7.515398493340021E-4 5.980423204249354E-6 46-47 0.15752036025112515 1.395432080991516E-5 0.0 7.90412600161623E-4 5.980423204249354E-6 48-49 0.15866262108313678 1.395432080991516E-5 0.0 8.123408185772039E-4 5.980423204249354E-6 50-51 0.1602045735326324 1.395432080991516E-5 0.0 8.332722997920766E-4 5.980423204249354E-6 52-53 0.16199371680790364 1.395432080991516E-5 0.0 8.552005182076575E-4 5.980423204249354E-6 54-55 0.16330841317563782 1.395432080991516E-5 0.0 8.781254738239469E-4 7.973897605665805E-6 56-57 0.1649291078639894 1.395432080991516E-5 0.0 8.880928458310291E-4 7.973897605665805E-6 58-59 0.16765119715912358 1.395432080991516E-5 0.0 9.120145386480264E-4 7.973897605665805E-6 60-61 0.17118562727283493 1.395432080991516E-5 0.0 9.31949282662191E-4 7.973897605665805E-6 62-63 0.17556927748154968 1.395432080991516E-5 0.0 9.508872894756473E-4 7.973897605665805E-6 64-65 0.18000675149910272 1.395432080991516E-5 0.0 9.758057194933529E-4 7.973897605665805E-6 66-67 0.18771352353497872 1.395432080991516E-5 0.0 0.0010037143611131834 8.97063480637403E-6 68-69 0.19559173436937655 1.395432080991516E-5 0.0 0.0010236491051273478 9.967372007082256E-6 70-71 0.19815135550079527 1.395432080991516E-5 0.0 0.0010395969003386793 9.967372007082256E-6 72-73 0.19995644657127787 1.395432080991516E-5 0.0 0.001051557746747178 9.967372007082256E-6 74-75 0.20539265126394052 1.395432080991516E-5 0.0 0.001086443548771966 9.967372007082256E-6 76-77 0.21661092845791158 1.395432080991516E-5 0.0 0.0012379476032796162 9.967372007082256E-6 78-79 0.22187469761485173 1.395432080991516E-5 0.0 0.0012867877261143195 1.1960846408498708E-5 80-81 0.23335013300660554 1.395432080991516E-5 0.0 0.0013545658557624785 1.1960846408498708E-5 82-83 0.24125126879661965 1.395432080991516E-5 0.0 0.0013844679717837254 1.395432080991516E-5 84-85 0.2672990020627277 1.395432080991516E-5 0.0 0.0014193537738085133 1.395432080991516E-5 86-87 0.3622830736042181 1.395432080991516E-5 0.0 0.0014502526270304683 1.395432080991516E-5 88-89 0.4971356364368368 1.594779521133161E-5 0.0 0.0014612167362382587 1.395432080991516E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 38645 0.0 61.705475 1 CCCGAAA 17155 0.0 51.44797 1 GCGTGTT 50855 0.0 46.78684 2 GTTTTTT 716715 0.0 45.96948 1 CGGGCTA 2565 0.0 38.520267 1 CCGAAAC 24215 0.0 36.270935 2 CGCCGTA 11110 0.0 36.175343 46-47 GTCGCCG 12450 0.0 34.43451 44-45 CGTGTTT 72690 0.0 33.092182 3 TCGTCTA 22815 0.0 32.670418 44-45 CGAGCTA 23205 0.0 32.400322 12-13 ACTCGTC 23065 0.0 32.170246 42-43 CGAAACC 27530 0.0 31.937916 3 CCCAGAT 34005 0.0 31.486504 1 CTAACGA 22480 0.0 31.29292 94-95 CCGTATC 13045 0.0 31.22894 48-49 TCGGTGG 15515 0.0 30.670671 38-39 CGGCCTT 6260 0.0 29.821638 1 GATCTCG 15320 0.0 29.03797 34-35 CAAAACG 57005 0.0 28.976168 9 >>END_MODULE