FastQCFastQC Report
Fri 27 May 2016
SRR1233043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50163674
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1266490.2524715394649922No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1211680.24154530627082857No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1125860.2244373089578726No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT1015130.20236356691098822No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC832640.1659846525595394No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC819670.16339911626090226No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT745380.14858959493277946No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT691040.13775705503548244No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC678340.13522534254568355TruSeq Adapter, Index 11 (100% over 50bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA651400.12985492250826763No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC614350.1224690998510197No Hit
CTACATTTTCTTATAAAAGAACATTACTATACCCTTTATGAAACTAAAGG596860.11898251312294232No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA570200.11366791036876604No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC560330.11170035113456801No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA554440.11052619471213372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT436000.065.662681
CCCGAAA165650.051.8856121
GCGTGTT564750.050.5498542
GTTTTTT6605600.040.5396921
CGGGCTA21900.039.0290681
TATGCCG252750.037.9131446-47
CCGAAAC230800.037.0745772
TGCCGTC260950.036.46832348-49
CGTGTTT804350.035.8744623
CTCGTAT269950.034.9709342-43
CCGTCTT277700.034.3900450-51
CGAAACC258250.033.1142233
AGTCACG141400.032.46980728-29
CGAGCTA223250.032.4020312-13
CGTCTGA301450.032.3354216-17
CCCAGAT328900.032.3114171
TCGTCTA220000.032.196544-45
CTAACGA210700.031.54333194-95
ACTCGTC225250.031.49632542-43
GCGACCG7600.029.9888464
Produced by FastQC (version 0.11.5)