##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233043_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50163674 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10835601873978 33.0 31.0 34.0 30.0 34.0 2 32.40293478105292 34.0 31.0 34.0 31.0 34.0 3 32.23108024344469 34.0 31.0 34.0 30.0 34.0 4 35.86596653586418 37.0 35.0 37.0 35.0 37.0 5 35.85485692295983 37.0 35.0 37.0 35.0 37.0 6 35.86281258027472 37.0 35.0 37.0 35.0 37.0 7 35.96253980519848 37.0 35.0 37.0 35.0 37.0 8 35.947493339503005 37.0 35.0 37.0 35.0 37.0 9 37.675458819064964 39.0 37.0 39.0 35.0 39.0 10-11 37.59758287441227 39.0 37.0 39.0 35.0 39.0 12-13 37.55280253196766 39.0 37.0 39.0 35.0 39.0 14-15 38.87310586541169 40.0 38.0 41.0 35.0 41.0 16-17 38.701650241965936 40.0 38.0 41.0 34.0 41.0 18-19 38.47043361497007 40.0 38.0 41.0 34.0 41.0 20-21 38.238127265160045 40.0 37.5 41.0 33.5 41.0 22-23 38.05268417939244 40.0 37.0 41.0 33.0 41.0 24-25 37.90120683744178 40.0 38.0 41.0 33.0 41.0 26-27 37.468400829253454 40.0 37.0 41.0 32.5 41.0 28-29 37.07029441264609 40.0 37.0 41.0 31.5 41.0 30-31 36.734803934018075 40.0 37.0 41.0 30.5 41.0 32-33 36.4892503886378 40.0 37.0 41.0 30.0 41.0 34-35 36.31705884421464 40.0 37.0 41.0 30.0 41.0 36-37 36.18427378345534 40.0 37.0 41.0 29.0 41.0 38-39 36.06602296514406 40.0 37.0 41.0 28.5 41.0 40-41 35.95531242388665 40.0 37.0 41.0 27.5 41.0 42-43 35.84492021058904 40.0 36.0 41.0 27.0 41.0 44-45 35.751752363672566 40.0 36.0 41.0 27.0 41.0 46-47 35.758938400325306 40.0 36.0 41.0 27.0 41.0 48-49 35.73584690985752 40.0 36.0 41.0 27.0 41.0 50-51 35.64820750569426 40.0 36.0 41.0 26.0 41.0 52-53 35.50556981731442 40.0 36.0 41.0 26.0 41.0 54-55 35.40285996595864 40.0 36.0 41.0 25.5 41.0 56-57 35.2655180320325 39.5 35.5 41.0 25.0 41.0 58-59 35.0791972394207 39.0 35.0 41.0 24.5 41.0 60-61 34.87428330109952 39.0 35.0 41.0 23.5 41.0 62-63 34.63577486369918 39.0 35.0 40.5 23.0 41.0 64-65 34.35292264478076 38.5 34.0 40.0 22.0 41.0 66-67 34.0842292970806 38.0 34.0 40.0 21.5 41.0 68-69 33.770583779409776 37.5 34.0 40.0 20.5 41.0 70-71 33.389985031798105 37.0 34.0 40.0 18.5 41.0 72-73 33.0492764844138 36.5 33.5 39.0 18.0 41.0 74-75 32.666965551606125 36.0 33.0 39.0 15.5 40.5 76-77 31.95239397736298 35.0 32.0 38.0 13.0 40.0 78-79 31.999228934068903 35.0 32.5 37.5 12.0 39.5 80-81 31.71377454330797 35.0 32.0 37.0 8.0 39.0 82-83 31.363627153784627 35.0 32.0 37.0 7.0 39.0 84-85 30.979446840357028 35.0 32.0 36.0 4.5 38.0 86-87 30.58730037397181 35.0 31.5 36.0 2.0 37.0 88-89 30.3417133382216 35.0 31.0 35.5 2.0 37.0 90-91 30.075697854586966 35.0 31.0 35.0 2.0 36.5 92-93 29.751210068863777 34.0 31.0 35.0 2.0 36.0 94-95 29.426574596589553 34.0 31.0 35.0 2.0 36.0 96-97 29.085769126479853 34.0 31.0 35.0 2.0 36.0 98-99 28.267783346570667 34.0 29.5 35.0 2.0 35.0 100-101 26.08482602370791 32.0 24.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3562620092923936 1101 2 0.22924435335345095 1101 3 0.5449942055309691 1101 4 0.33628844098703325 1101 5 0.24640053269794748 1101 6 0.2343225068574597 1101 7 0.1913945474443679 1101 8 0.20731548080527773 1101 9 0.21742440803264884 1101 10-11 0.2091899532417898 1101 12-13 0.21635652113864978 1101 14-15 0.28526646687204504 1101 16-17 0.36039225506485906 1101 18-19 0.3691476510308007 1101 20-21 0.37410063242033686 1101 22-23 0.41970620821999916 1101 24-25 0.4015967282620636 1101 26-27 0.4319338753279425 1101 28-29 0.43985076295965087 1101 30-31 0.3977849229968129 1101 32-33 0.4114024979250175 1101 34-35 0.3912625589277994 1101 36-37 0.37580570722937523 1101 38-39 0.3810668991141384 1101 40-41 0.31267102811379743 1101 42-43 0.297857243508318 1101 44-45 0.34675836822121653 1101 46-47 0.4133042628318506 1101 48-49 0.44745804630003505 1101 50-51 0.4513007515528429 1101 52-53 0.47228524305026554 1101 54-55 0.457957915192722 1101 56-57 0.44964127070475257 1101 58-59 0.45121706458339617 1101 60-61 0.4763147565444754 1101 62-63 0.46740689443694095 1101 64-65 0.4203024221929468 1101 66-67 0.4077783253043208 1101 68-69 0.40924652547693796 1101 70-71 0.42342090822539546 1101 72-73 0.47017698822653387 1101 74-75 0.44782779509980486 1101 76-77 0.49145574358411537 1101 78-79 0.48142645510640847 1101 80-81 0.44154915566565833 1101 82-83 0.3918437366033025 1101 84-85 0.32387486425753664 1101 86-87 0.34114346644046023 1101 88-89 0.4053716385289938 1101 90-91 0.37214815759628195 1101 92-93 0.3607182212416262 1101 94-95 0.4109754826022751 1101 96-97 0.37921343553747633 1101 98-99 0.35510532029898556 1101 100-101 0.7163175314742354 1102 1 0.32565357125206873 1102 2 0.2202340768997857 1102 3 0.5227231740270426 1102 4 0.3307740467689726 1102 5 0.208540253301841 1102 6 0.21690221868041704 1102 7 0.18946652620020643 1102 8 0.20895093079175098 1102 9 0.270994804869936 1102 10-11 0.23373400928834087 1102 12-13 0.2247045764219351 1102 14-15 0.2660107523104642 1102 16-17 0.27988786109163755 1102 18-19 0.33751419325094645 1102 20-21 0.3299670849319014 1102 22-23 0.32134599500542294 1102 24-25 0.3514733092211273 1102 26-27 0.38730818145415213 1102 28-29 0.39046379387142593 1102 30-31 0.33716570453299965 1102 32-33 0.3321883569732691 1102 34-35 0.3579912406268235 1102 36-37 0.3933785540834762 1102 38-39 0.3580669739804989 1102 40-41 0.3125869050727701 1102 42-43 0.30966710279054865 1102 44-45 0.32972415333628646 1102 46-47 0.3880766394444848 1102 48-49 0.3956735332029595 1102 50-51 0.4366891335538483 1102 52-53 0.4133526449729885 1102 54-55 0.39077445892414175 1102 56-57 0.4149970869818205 1102 58-59 0.4197697041136337 1102 60-61 0.4451582499200768 1102 62-63 0.42472394191975127 1102 64-65 0.3591975132414902 1102 66-67 0.4110984592200495 1102 68-69 0.37397102110823965 1102 70-71 0.31414967121860116 1102 72-73 0.39500018701390616 1102 74-75 0.36710540874872777 1102 76-77 0.37614613892865023 1102 78-79 0.3723711829074787 1102 80-81 0.44374251378566854 1102 82-83 0.3942453068373837 1102 84-85 0.3137399348512737 1102 86-87 0.31083234708806273 1102 88-89 0.33240036584886923 1102 90-91 0.3566263851202578 1102 92-93 0.42144653428778867 1102 94-95 0.44518806558895463 1102 96-97 0.41361639958811836 1102 98-99 0.24782417122699485 1102 100-101 0.6142720022827284 1103 1 0.31053804529691575 1103 2 0.2488623303904518 1103 3 0.5241554234504804 1103 4 0.3238814078249064 1103 5 0.22669736762646409 1103 6 0.19677021859678234 1103 7 0.17314537133243846 1103 8 0.15323917759572936 1103 9 0.22042066966779572 1103 10-11 0.20703297610034355 1103 12-13 0.2119703765534382 1103 14-15 0.21802716899112795 1103 16-17 0.2883939029587168 1103 18-19 0.2918940825329486 1103 20-21 0.2796521571649251 1103 22-23 0.2879091209304434 1103 24-25 0.27992977950081155 1103 26-27 0.24356637309806928 1103 28-29 0.2691935513786703 1103 30-31 0.2398037574454932 1103 32-33 0.20686498153386168 1103 34-35 0.23189668800025487 1103 36-37 0.25204251657471133 1103 38-39 0.2515238979559129 1103 40-41 0.1987007443353761 1103 42-43 0.18119374528582455 1103 44-45 0.22154443339342578 1103 46-47 0.2661714644914852 1103 48-49 0.2532484188807729 1103 50-51 0.29275730077939954 1103 52-53 0.26958598180461735 1103 54-55 0.298230167766377 1103 56-57 0.292211892524989 1103 58-59 0.2793016606343599 1103 60-61 0.3213960019115163 1103 62-63 0.2850680300410673 1103 64-65 0.2769226868993684 1103 66-67 0.22344050560906936 1103 68-69 0.2592797585166622 1103 70-71 0.23636407584496055 1103 72-73 0.30753595437578696 1103 74-75 0.2795913061193005 1103 76-77 0.2836061614327754 1103 78-79 0.27222768716867307 1103 80-81 0.31620195267671036 1103 82-83 0.23575499917890141 1103 84-85 0.21585059438683274 1103 86-87 0.22225222594419947 1103 88-89 0.28745814354364185 1103 90-91 0.2593544017350631 1103 92-93 0.2558134298052117 1103 94-95 0.2713574039178823 1103 96-97 0.2704835970618795 1103 98-99 0.20190685428002553 1103 100-101 0.4345757226486313 1104 1 0.3112732514506007 1104 2 0.2615904545487169 1104 3 0.4981679767495848 1104 4 0.3354636375669955 1104 5 0.2016565910253334 1104 6 0.19050346811113172 1104 7 0.15185500778632388 1104 8 0.16003404117923026 1104 9 0.19716190185139482 1104 10-11 0.20243634058376614 1104 12-13 0.21110543324898856 1104 14-15 0.2584047483285161 1104 16-17 0.2554755889160134 1104 18-19 0.2494083386539856 1104 20-21 0.30150471895633046 1104 22-23 0.2867198702798319 1104 24-25 0.2670588471427777 1104 26-27 0.24779658150381323 1104 28-29 0.2802589389830601 1104 30-31 0.28890343161398135 1104 32-33 0.2803909405226719 1104 34-35 0.25967747758922854 1104 36-37 0.28036250871036117 1104 38-39 0.2613759233427615 1104 40-41 0.262973815565239 1104 42-43 0.2594470212475599 1104 44-45 0.24984962200113614 1104 46-47 0.25671885782821136 1104 48-49 0.23742238281380423 1104 50-51 0.2534802303108421 1104 52-53 0.2651353341845848 1104 54-55 0.2346963209124482 1104 56-57 0.24502349751958263 1104 58-59 0.22126426998290327 1104 60-61 0.2823397377405641 1104 62-63 0.2761376274088576 1104 64-65 0.23962297444783331 1104 66-67 0.26402732527724737 1104 68-69 0.2866448504147314 1104 70-71 0.26197713248838284 1104 72-73 0.21445574688713975 1104 74-75 0.24239063631895164 1104 76-77 0.2605264691601725 1104 78-79 0.2786739718492228 1104 80-81 0.3873641008029445 1104 82-83 0.28425531989561037 1104 84-85 0.2713016589509749 1104 86-87 0.276827510936986 1104 88-89 0.3271059604429425 1104 90-91 0.32976766335715624 1104 92-93 0.3928617994190944 1104 94-95 0.41540610590864446 1104 96-97 0.45233347898266985 1104 98-99 0.3302426809441954 1104 100-101 0.5136203021191861 1105 1 0.20341120958329384 1105 2 0.1605781825940369 1105 3 0.43747928386696344 1105 4 0.2830057794500789 1105 5 0.17475365100393248 1105 6 0.11518940529103361 1105 7 0.04116007856361392 1105 8 0.054610172581405436 1105 9 0.11346510728868964 1105 10-11 0.09677512006251021 1105 12-13 0.11639862184705407 1105 14-15 0.07769643050795594 1105 16-17 0.14714555658443373 1105 18-19 0.19917259792260467 1105 20-21 0.17098240373680795 1105 22-23 0.15230606718412076 1105 24-25 0.10471987456212872 1105 26-27 0.14488229905401084 1105 28-29 0.10553934545087174 1105 30-31 0.09871512675656646 1105 32-33 0.12518824719540333 1105 34-35 0.11657349749972923 1105 36-37 0.10754862104797525 1105 38-39 0.11405649004586138 1105 40-41 0.09636509666666626 1105 42-43 0.10508444198252676 1105 44-45 0.12619753882349016 1105 46-47 0.18074858038932717 1105 48-49 0.16172050923769632 1105 50-51 0.17981285242093037 1105 52-53 0.15159316857883454 1105 54-55 0.19571287116858826 1105 56-57 0.18329934606411769 1105 58-59 0.18740881199009607 1105 60-61 0.20829115119696695 1105 62-63 0.22081935953857368 1105 64-65 0.2042855939241761 1105 66-67 0.2159115270747307 1105 68-69 0.21619464840812697 1105 70-71 0.20712808707159525 1105 72-73 0.20493318002596794 1105 74-75 0.24694743236884875 1105 76-77 0.22281180563849645 1105 78-79 0.20833269622948336 1105 80-81 0.29326046641953596 1105 82-83 0.24889123368817323 1105 84-85 0.19148235842448358 1105 86-87 0.24691554981933095 1105 88-89 0.24733374372573635 1105 90-91 0.23902502462564712 1105 92-93 0.2628425807233299 1105 94-95 0.282831038911155 1105 96-97 0.29784916542619655 1105 98-99 0.2496443841288638 1105 100-101 0.23829670200666442 1106 1 0.14505325991067508 1106 2 0.11388972918485507 1106 3 0.3779341662137128 1106 4 0.24043882022824192 1106 5 0.12871300169046407 1106 6 0.062134524020066806 1106 7 0.048076359181692396 1106 8 0.058920015102714274 1106 9 0.07876544953125375 1106 10-11 0.045615665210178236 1106 12-13 0.03280597943427921 1106 14-15 0.049035352788237674 1106 16-17 0.015082277710945391 1106 18-19 0.043075744387813586 1106 20-21 0.0681379067924226 1106 22-23 0.07861835913050186 1106 24-25 0.06381319561634058 1106 26-27 0.05987440093647223 1106 28-29 0.07483373550977745 1106 30-31 0.06217082421309783 1106 32-33 0.04741520337923788 1106 34-35 0.056076366028307234 1106 36-37 0.07289623930724787 1106 38-39 0.0817080557293437 1106 40-41 0.07235345653730718 1106 42-43 0.075105893531763 1106 44-45 0.05989963157770717 1106 46-47 0.03813452830222985 1106 48-49 0.06207245751822654 1106 50-51 0.04507412670432842 1106 52-53 0.04546416468934211 1106 54-55 0.03717148318774832 1106 56-57 0.05537218141512312 1106 58-59 0.07351960752863818 1106 60-61 0.10229925034840193 1106 62-63 0.0713182631775382 1106 64-65 0.06833782034201619 1106 66-67 0.08840043180789081 1106 68-69 0.11604629125097432 1106 70-71 0.14005344624260374 1106 72-73 0.12407178503569583 1106 74-75 0.16785132163072802 1106 76-77 0.11738755270602397 1106 78-79 0.0863958114826957 1106 80-81 0.16485560248230513 1106 82-83 0.09314519352900419 1106 84-85 0.16638049525342424 1106 86-87 0.19404803957718997 1106 88-89 0.18060764068078328 1106 90-91 0.1777798164329063 1106 92-93 0.24168557838465432 1106 94-95 0.20065458288112126 1106 96-97 0.2343831111463892 1106 98-99 0.1231252423806275 1106 100-101 0.29599665712597556 1107 1 0.1175667997058909 1107 2 0.08004020126598732 1107 3 0.33472681780583 1107 4 0.21042839295525084 1107 5 0.07615972000233029 1107 6 0.05359179182408269 1107 7 0.030151046568256845 1107 8 0.025542145586882725 1107 9 0.03519281178963496 1107 10-11 0.008939021429476668 1107 12-13 -0.008880431222912932 1107 14-15 -0.007931267310155476 1107 16-17 -0.0050352334806547105 1107 18-19 0.0060404639210602795 1107 20-21 0.04604707565228239 1107 22-23 0.04672935191371863 1107 24-25 0.013437975652500711 1107 26-27 0.0043961112199326635 1107 28-29 -0.032083170611606704 1107 30-31 -0.05437077734787721 1107 32-33 -0.014339421367601801 1107 34-35 -0.05414621940132491 1107 36-37 -0.028335510239863027 1107 38-39 0.004832959277358384 1107 40-41 -0.017424653711053395 1107 42-43 -0.018554441099105645 1107 44-45 0.01796228354587015 1107 46-47 -0.016850148357768546 1107 48-49 -0.043277772979010365 1107 50-51 -0.0636231195888044 1107 52-53 0.007700731068815969 1107 54-55 0.0016115664308955502 1107 56-57 0.0016899675296926375 1107 58-59 0.005994479993503887 1107 60-61 0.04075411744643276 1107 62-63 0.07805352472980331 1107 64-65 -0.05186925499994288 1107 66-67 -0.010140935496814052 1107 68-69 -0.01414488094865618 1107 70-71 0.001192860043531141 1107 72-73 0.04785713555779125 1107 74-75 0.06796689446644422 1107 76-77 0.038304300878355235 1107 78-79 0.03365929788104438 1107 80-81 0.10990024144822996 1107 82-83 0.05138416769664644 1107 84-85 0.08621519018198498 1107 86-87 0.054672354531245304 1107 88-89 0.10340539035851393 1107 90-91 0.053698252771400945 1107 92-93 0.13995535828650674 1107 94-95 0.12131315414377042 1107 96-97 0.15918567616330748 1107 98-99 0.06593368346119632 1107 100-101 0.06250511009548632 1108 1 0.07106787585130547 1108 2 0.032527023618861506 1108 3 0.2767416941068035 1108 4 0.17034062024490026 1108 5 0.040841875944792605 1108 6 -0.004245560782877078 1108 7 -1.9439794788667086E-4 1108 8 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4414.0 3 725.0 4 1951.0 5 5661.0 6 18355.0 7 64019.0 8 211144.0 9 447413.0 10 717955.0 11 683762.0 12 460000.0 13 288202.0 14 210584.0 15 177127.0 16 162130.0 17 152551.0 18 147862.0 19 150828.0 20 157029.0 21 166976.0 22 186486.0 23 213455.0 24 249971.0 25 297720.0 26 360112.0 27 434039.0 28 531159.0 29 666959.0 30 840034.0 31 1057604.0 32 1341853.0 33 1726881.0 34 2301909.0 35 3240895.0 36 4997169.0 37 9106962.0 38 1.3616141E7 39 4760894.0 40 4743.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.561890751837925 17.13371946171155 10.172635388978854 44.131754397471674 2 14.570358206304723 17.864396309245812 53.69508413107352 13.870161353375945 3 19.331740502085324 21.604360804645385 37.7857944520456 21.278104241223687 4 20.30523322514216 27.78894344939727 34.16184388727189 17.743979438188678 5 20.246419579920502 31.500657564100287 34.50033002800304 13.752592827976168 6 15.150981564867038 34.89705319430949 35.81951752576975 14.132447715053726 7 12.342742967991468 19.036918262833918 54.23311558936446 14.387223179810164 8 13.05519187020282 22.577086536934743 46.76718436965135 17.60053722321108 9 14.478412198225996 21.517282359794297 46.455122112613736 17.549183329365974 10-11 17.02539072397289 32.770203394591874 35.53730633844722 14.66709954298802 12-13 16.39278056069019 26.27570101823084 41.26298942936277 16.068528991716196 14-15 17.31613202974109 28.476959768126463 38.755484274353385 15.451423927779063 16-17 18.266634161043594 28.667788654881694 36.91034730472099 16.155229879353726 18-19 18.3790186500295 28.558125547183806 36.23664307363133 16.826212729155365 20-21 18.695885219946906 28.831151749407923 35.47692293155266 16.996040099092514 22-23 17.911724879476246 30.077223135549414 35.679755096012386 16.33129688896196 24-25 17.96770696099214 29.59770728742609 35.047201601370325 17.387384150211446 26-27 17.824542715910322 30.152027939580346 34.34224933365128 17.681180010858057 28-29 18.727059961873994 29.909179870631046 33.59249571487598 17.771264452618976 30-31 18.406312849601356 30.54996981031296 33.36590206692182 17.677815273163866 32-33 18.054003580359765 30.201682396708023 33.188760257073675 18.555553765858537 34-35 18.641389090537146 30.831374271147084 32.19471150939802 18.332525128917748 36-37 18.75690575653208 31.03934741681753 32.24204648296441 17.96170034368598 38-39 18.65369347832349 30.574485808199658 32.58879039099479 18.183030322482065 40-41 18.57633043942811 30.968648248724207 32.52499308287309 17.9300282289746 42-43 18.32793686476652 30.972140610583267 32.85558362756716 17.844338897083055 44-45 18.78854400698752 29.982743217823245 32.665422700952504 18.56329007423673 46-47 18.387652730083083 31.215714281627438 32.19372268871034 18.202910299579145 48-49 18.17531207216544 30.71384612262159 32.982176562766234 18.128665242446733 50-51 17.92565025186597 30.764238446578517 33.14747001248426 18.162641289071253 52-53 18.83983417324462 30.36978111561948 32.472407189892024 18.31797752124388 54-55 18.980661487109522 30.80896576828006 31.878336745352364 18.332035999258046 56-57 19.46703102825306 30.513672629503517 31.74539147810221 18.273904864141215 58-59 18.66751152713246 31.518374962168622 31.822227733530788 17.991885777168125 60-61 19.000958350161802 31.179845726566047 31.226475794428403 18.592720128843748 62-63 19.019290838389235 31.735212378273168 31.1086050768707 18.13689170646689 64-65 18.801953022217106 31.309318366297383 31.314816573467034 18.573912038018477 66-67 18.95175313489338 31.919189133706222 30.904983157565276 18.22407457383512 68-69 19.06234029049417 31.523194961812713 31.152437818261692 18.26202692943143 70-71 18.292209331050348 31.614848670807177 31.13669415858425 18.956247839558227 72-73 18.459539135328345 31.448724258531236 31.26108718307876 18.83064942306166 74-75 18.83715866321177 30.951912547286 31.556687977752457 18.654240811749776 76-77 18.4089198774141 31.686469411779427 31.07448315201679 18.830127558789677 78-79 18.665427323185625 32.36142503025724 30.666236709352578 18.306910937204556 80-81 18.499478859216055 32.38286017623969 31.07972206702248 18.037938897521784 82-83 19.22210973192638 31.965304807871487 30.595005585058114 18.217579875144015 84-85 18.825640702582145 31.917294581946663 30.817210917953247 18.439853797517944 86-87 18.732291534477984 32.36399971250363 30.859759689320498 18.04394906369789 88-89 18.83131623155431 31.808214672987244 30.956912653341572 18.403556442116876 90-91 18.39872878579255 32.64445062770149 30.410744922697912 18.54607566380805 92-93 19.260284206902682 31.843044743636444 30.58628476819753 18.310386281263344 94-95 19.14506264261113 32.18548048297478 30.067977857965555 18.60147901644853 96-97 18.863902325298945 32.81156317249474 30.32729075999724 17.997243742209072 98-99 19.780402418589947 31.81702383864362 29.890726443350026 18.511847299416406 100-101 19.746486321901298 32.893807066549556 28.968813154605666 18.39089345694348 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 24141.0 1 25420.0 2 25046.5 3 27523.0 4 37374.0 5 49526.0 6 62022.5 7 74592.5 8 87891.0 9 101524.0 10 114423.5 11 125992.0 12 137822.5 13 149345.5 14 162277.0 15 176103.0 16 188695.5 17 202561.5 18 223073.5 19 251722.0 20 291819.5 21 345986.5 22 417021.0 23 504031.5 24 610701.5 25 768419.5 26 919252.0 27 1107481.5 28 1252709.5 29 1368534.0 30 1543475.0 31 1659826.0 32 1778639.5 33 1920115.0 34 2057147.0 35 2163909.5 36 2303217.0 37 2378650.5 38 2380711.5 39 2299773.5 40 2253454.0 41 2255542.0 42 1964547.5 43 1637802.5 44 1448818.5 45 1297580.0 46 1182689.5 47 1088958.0 48 1033837.5 49 961146.5 50 822617.0 51 679054.0 52 585705.5 53 515113.0 54 431433.0 55 346382.5 56 285873.5 57 248165.0 58 214703.5 59 174288.0 60 125477.0 61 83792.5 62 59540.5 63 43426.0 64 30383.5 65 20296.0 66 13794.5 67 10127.0 68 7473.5 69 5159.5 70 4096.0 71 4033.0 72 4166.5 73 4804.5 74 4033.5 75 2258.0 76 1793.0 77 1679.0 78 1253.5 79 691.5 80 454.0 81 316.0 82 184.5 83 103.0 84 60.0 85 48.0 86 38.0 87 23.0 88 12.0 89 5.5 90 3.0 91 2.0 92 1.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 1.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004911920925090136 2 0.002695177390715042 3 0.0020034417734235335 4 0.0 5 0.0025197516433903945 6 0.0 7 3.7876013626912574E-5 8 0.00404475956047398 9 3.648058154592106E-4 10-11 0.0 12-13 0.0 14-15 0.0023084433568402504 16-17 9.070308526444853E-5 18-19 0.0 20-21 8.97063480637403E-5 22-23 3.0898853221955E-5 24-25 7.704778561474584E-4 26-27 0.0 28-29 2.9902116021246768E-5 30-31 4.086622522903725E-5 32-33 0.0 34-35 6.97716040495758E-6 36-37 0.013349301329085265 38-39 0.001453242838632593 40-41 2.8805705100467724E-4 42-43 0.0012399410776810327 44-45 0.00676884233000956 46-47 0.005493018713103031 48-49 0.011810339091191766 50-51 0.020954406170489027 52-53 0.04735598114284851 54-55 0.04590871872742016 56-57 0.05169776838913354 58-59 0.05656184592858968 60-61 0.050682093181611855 62-63 0.06513677606628256 64-65 0.03998112259480834 66-67 0.023779159397296138 68-69 0.04161876181557196 70-71 0.041011748860340654 72-73 0.03575096991500264 74-75 0.03249263600588745 76-77 0.03348339278339142 78-79 0.053652370039722376 80-81 0.029975874574099177 82-83 0.0203573605872648 84-85 0.020093225229077118 86-87 0.012995459622833844 88-89 0.00863373763253465 90-91 0.0062335944532292435 92-93 0.009275636389790747 94-95 0.008624766997728276 96-97 0.008691548390175727 98-99 0.21888049906392423 100-101 0.06524641715836045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.0163674E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.71341011653352 #Duplication Level Percentage of deduplicated Percentage of total 1 77.89707092939281 19.251022607547856 2 9.352242185460822 4.622515932768781 3 3.5689787132902153 2.646049039161576 4 1.9439983617890677 1.9217131512305017 5 1.2182028310835242 1.5052973084844672 6 0.8710618382929519 1.2916145067957194 7 0.6243683565773992 1.0801189881918314 8 0.49840619465079256 0.9853853354420699 9 0.3797422676398324 0.8446253758889053 >10 2.902332105728138 14.392616125773591 >50 0.3792387967794148 6.550905241708296 >100 0.30224250117083185 14.883547848950599 >500 0.03411638198600989 5.809553113788359 >1k 0.02387234534856556 11.429781633632809 >5k 0.0025910529487525352 4.4848217012232245 >10k+ 0.0015351378611341894 8.300432089411581 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 126649 0.2524715394649922 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 121168 0.24154530627082857 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 112586 0.2244373089578726 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 101513 0.20236356691098822 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 83264 0.1659846525595394 No Hit GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC 81967 0.16339911626090226 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 74538 0.14858959493277946 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69104 0.13775705503548244 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC 67834 0.13522534254568355 TruSeq Adapter, Index 11 (100% over 50bp) AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA 65140 0.12985492250826763 No Hit CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC 61435 0.1224690998510197 No Hit CTACATTTTCTTATAAAAGAACATTACTATACCCTTTATGAAACTAAAGG 59686 0.11898251312294232 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 57020 0.11366791036876604 No Hit ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC 56033 0.11170035113456801 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 55444 0.11052619471213372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.12464398042296503 0.0 0.0 1.1960846408498708E-5 0.0 2 0.12521212062736872 5.980423204249354E-6 0.0 1.794126961274806E-5 0.0 3 0.1252579705386013 5.980423204249354E-6 0.0 2.5915167218413868E-5 0.0 4 0.12641019874262 5.980423204249354E-6 0.0 2.9902116021246768E-5 1.9934744014164512E-6 5 0.1266514091451914 5.980423204249354E-6 0.0 4.186296242974548E-5 1.9934744014164512E-6 6 0.1269045803941713 5.980423204249354E-6 0.0 4.784338563399483E-5 3.9869488028329024E-6 7 0.12783353946523135 5.980423204249354E-6 0.0 7.77455016552416E-5 3.9869488028329024E-6 8 0.1410203726306012 5.980423204249354E-6 0.0 8.372592485949096E-5 3.9869488028329024E-6 9 0.14123168091715133 5.980423204249354E-6 0.0 8.97063480637403E-5 3.9869488028329024E-6 10-11 0.14341353864950163 5.980423204249354E-6 0.0 9.668350846869789E-5 3.9869488028329024E-6 12-13 0.14529139153563592 5.980423204249354E-6 0.0 1.0166719447223901E-4 5.980423204249354E-6 14-15 0.14668084319342317 5.980423204249354E-6 0.0 1.1362804088073772E-4 5.980423204249354E-6 16-17 0.14771445967055763 5.980423204249354E-6 0.0 1.32566047694194E-4 5.980423204249354E-6 18-19 0.1487052164480616 5.980423204249354E-6 0.0 1.7741922172606416E-4 5.980423204249354E-6 20-21 0.15011360611266233 5.980423204249354E-6 0.0 2.172887097543932E-4 5.980423204249354E-6 22-23 0.15175523228222876 5.980423204249354E-6 0.0 2.4320387697280703E-4 5.980423204249354E-6 24-25 0.15311079487519197 5.980423204249354E-6 0.0 2.9403747420892655E-4 5.980423204249354E-6 26-27 0.15446735420535584 5.980423204249354E-6 0.0 4.086622522903725E-4 5.980423204249354E-6 28-29 0.15587076018395302 5.980423204249354E-6 0.0 4.6447953553003314E-4 5.980423204249354E-6 30-31 0.15724725425813108 6.977160404957579E-6 0.0 5.183033443682773E-4 5.980423204249354E-6 32-33 0.1582878478956705 7.973897605665805E-6 0.0 5.312609279774843E-4 5.980423204249354E-6 34-35 0.1589805802501627 7.973897605665805E-6 0.0 5.52192409192357E-4 5.980423204249354E-6 36-37 0.1593304350076113 7.973897605665805E-6 0.0 5.781075764107709E-4 5.980423204249354E-6 38-39 0.15958360625659118 7.973897605665805E-6 0.0 5.830912624143121E-4 5.980423204249354E-6 40-41 0.15987664699359938 7.973897605665805E-6 0.0 5.980423204249354E-4 5.980423204249354E-6 42-43 0.16051455880205268 7.973897605665805E-6 0.0 6.927323544922168E-4 7.973897605665805E-6 44-45 0.16151827316316586 7.973897605665805E-6 0.0 7.316051053198377E-4 7.973897605665805E-6 46-47 0.16236549978376785 7.973897605665805E-6 0.0 7.734680677495831E-4 7.973897605665805E-6 48-49 0.16334529245206403 7.973897605665805E-6 0.0 8.013767093694134E-4 7.973897605665805E-6 50-51 0.16488824163876037 7.973897605665805E-6 0.0 8.203147161828697E-4 7.973897605665805E-6 52-53 0.16665944364441887 7.973897605665805E-6 0.0 8.272918765878272E-4 7.973897605665805E-6 54-55 0.16785851849687086 7.973897605665805E-6 0.0 8.39252722996326E-4 7.973897605665805E-6 56-57 0.16939847747196507 7.973897605665805E-6 0.0 8.502168322041165E-4 7.973897605665805E-6 58-59 0.17217937426194102 7.973897605665805E-6 0.0 8.79122211024655E-4 7.973897605665805E-6 60-61 0.17545664617786966 7.973897605665805E-6 0.0 8.980602178381113E-4 7.973897605665805E-6 62-63 0.17989810714422552 8.97063480637403E-6 0.0 9.239753850565251E-4 7.973897605665805E-6 64-65 0.18486484861535463 9.967372007082256E-6 0.0 9.578644498806049E-4 7.973897605665805E-6 66-67 0.19263541183207594 9.967372007082256E-6 0.0 9.768024566940612E-4 7.973897605665805E-6 68-69 0.20062127028415022 9.967372007082256E-6 0.0 9.917535147046844E-4 7.973897605665805E-6 70-71 0.20316893056916047 1.1960846408498708E-5 0.0 9.977339379089339E-4 7.973897605665805E-6 72-73 0.2049660477420374 1.1960846408498708E-5 0.0 0.0010136817331202655 7.973897605665805E-6 74-75 0.21041122306950644 1.1960846408498708E-5 0.0 0.001042587111940804 7.973897605665805E-6 76-77 0.22201125061134874 1.1960846408498708E-5 0.0 0.0011602021016243746 7.973897605665805E-6 78-79 0.22737967717436325 1.1960846408498708E-5 0.0 0.0011990748524519953 7.973897605665805E-6 80-81 0.23918702605395292 1.1960846408498708E-5 0.0 0.001223993282469701 7.973897605665805E-6 82-83 0.24739416016458443 1.2957583609206934E-5 0.0 0.001250905186888823 7.973897605665805E-6 84-85 0.2741715050616109 1.395432080991516E-5 0.0 0.0012768203541072371 7.973897605665805E-6 86-87 0.3706915486293927 1.395432080991516E-5 0.0 0.0013017387841249427 7.973897605665805E-6 88-89 0.5077060344503475 1.395432080991516E-5 0.0 0.0013216735281391073 7.973897605665805E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 43600 0.0 65.66268 1 CCCGAAA 16565 0.0 51.885612 1 GCGTGTT 56475 0.0 50.549854 2 GTTTTTT 660560 0.0 40.539692 1 CGGGCTA 2190 0.0 39.029068 1 TATGCCG 25275 0.0 37.91314 46-47 CCGAAAC 23080 0.0 37.074577 2 TGCCGTC 26095 0.0 36.468323 48-49 CGTGTTT 80435 0.0 35.874462 3 CTCGTAT 26995 0.0 34.97093 42-43 CCGTCTT 27770 0.0 34.39004 50-51 CGAAACC 25825 0.0 33.114223 3 AGTCACG 14140 0.0 32.469807 28-29 CGAGCTA 22325 0.0 32.40203 12-13 CGTCTGA 30145 0.0 32.33542 16-17 CCCAGAT 32890 0.0 32.311417 1 TCGTCTA 22000 0.0 32.1965 44-45 CTAACGA 21070 0.0 31.543331 94-95 ACTCGTC 22525 0.0 31.496325 42-43 GCGACCG 760 0.0 29.988846 4 >>END_MODULE