FastQCFastQC Report
Fri 27 May 2016
SRR1233042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51841968
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1176400.22692039777502276No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC838590.16175890544895982Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT799320.1541839615347936No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA729460.1407083928603945No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720960.13906879461057498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT377050.064.48591
GCGTGTT499100.048.7362632
GTTTTTT8642750.042.042921
CGCCGTA221950.034.19674346-47
CCGTATC233950.032.69239848-49
GTCGCCG236900.032.57290644-45
CGTGTTT787400.031.445513
CAAAACG486250.030.2322399
TCGGTGG274050.029.2519938-39
CGGCCTT54250.028.0338951
TCTCGGT287100.027.25883736-37
TATGCGT73500.025.87527794-95
GATCTCG334500.025.44439734-35
CCGAGAT79350.024.6764971
CGGGCCT49500.024.5792121
GTGTTTT1972550.023.8889981
GTATGCG79000.023.80367794-95
TCGCCGT222800.023.15225646-47
GCCGTAT234050.023.07787348-49
GAGCGGC51400.022.946669
Produced by FastQC (version 0.11.5)