FastQCFastQC Report
Fri 27 May 2016
SRR1233042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51841968
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1027510.19820042325553688TruSeq Adapter, Index 8 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT894580.1725590355674769No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG879810.1697099924910258TruSeq Adapter, Index 8 (100% over 49bp)
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT804040.15509442079822278No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA701090.13523599258423213No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT595710.11490883216470485No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536930.10357052803242346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT377250.069.346021
GCGTGTT505950.052.428872
GTTTTTT7945300.041.4237941
CGTGTTT793950.033.8230633
TATGCCG370550.033.76634646-47
TGCCGTC377100.032.2793548-49
CCGTCTT396000.030.9572550-51
CTCGTAT388900.030.14677842-43
CGGCCTT49250.028.206141
ATCTCGT431550.026.75466540-41
CAAAACG428500.026.6079529
CGGGCCT42950.026.3621521
GTGTTTT2026950.026.2354281
CGTCTGA510150.026.17029416-17
CGGGCTT58200.026.157371
TATGCGT86000.024.4413994-95
CACGTCT567000.023.99859214-15
GAATCTC480050.023.88235938-39
CCAGTCA565900.023.44991126-27
CGGTGGT54250.023.3264681
Produced by FastQC (version 0.11.5)