FastQCFastQC Report
Fri 27 May 2016
SRR1233041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64079233
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT2889440.45091675800801173No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1994890.3113161482441589No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1892940.29540615756121796No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT1689920.263723506178671No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA1466340.22883232700366438No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1278150.19946399795390807Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1264520.19733694378021036Illumina Single End PCR Primer 1 (100% over 50bp)
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC1248940.19490557884798654No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA990950.15464448521098872No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC887570.1385113333051287No Hit
GCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAA854730.13338642801795084No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT849460.13256400868593418No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC842150.13142323348345947No Hit
GTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATC737660.1151168585304384No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT702450.10962209862905195No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT678820.10593447646291272No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC660160.1030224565890169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT449850.062.7922021
GCGTGTT605900.046.4403532
GTTTTTT9425550.044.585881
CGGCCTT92150.040.895211
ACCACCG65250.038.2360841
GAGCGGC79150.037.1044859
CCACCGA69600.036.5296862
CGCCGTA465450.035.8021846-47
CCGTATC480500.034.74146748-49
GTCGCCG489700.034.21567544-45
CCCGAAA127100.033.800061
ACCGAGA77400.032.9007954
CGTGTTT872500.032.611563
CGGGCCT70050.032.0883641
ATCGGGA64500.031.2397482
CAAAACG1070850.030.8998649
TCGGTGG552700.030.44854538-39
TCTCGGT537950.030.24365236-37
GGCGCCG58350.029.5551444-45
CGGCAAC44150.028.6315961
Produced by FastQC (version 0.11.5)