##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233041_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64079233
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28305742985407	34.0	31.0	34.0	31.0	34.0
2	32.489326456201496	34.0	33.0	34.0	31.0	34.0
3	32.51451602112653	34.0	33.0	34.0	31.0	34.0
4	35.76962767329003	37.0	37.0	37.0	35.0	37.0
5	35.745559953877724	37.0	37.0	37.0	35.0	37.0
6	35.73064585526484	37.0	37.0	37.0	35.0	37.0
7	35.763334167873076	37.0	37.0	37.0	35.0	37.0
8	35.74593241464048	37.0	37.0	37.0	35.0	37.0
9	37.47037065815067	39.0	39.0	39.0	35.0	39.0
10-11	37.42010882839375	39.0	39.0	39.0	35.0	39.0
12-13	37.37531202971796	39.0	39.0	39.0	35.0	39.0
14-15	38.82836434699523	41.0	40.0	41.0	36.0	41.0
16-17	38.592828147615315	41.0	39.0	41.0	35.0	41.0
18-19	38.170735618823656	41.0	38.5	41.0	34.0	41.0
20-21	37.826147458725046	41.0	38.0	41.0	33.5	41.0
22-23	37.47857045199027	41.0	38.0	41.0	32.5	41.0
24-25	37.097649756513164	41.0	38.0	41.0	31.5	41.0
26-27	36.650492594067096	40.0	38.0	41.0	29.0	41.0
28-29	36.35658380149463	41.0	38.0	41.0	26.5	41.0
30-31	36.11380342208528	41.0	38.0	41.0	24.0	41.0
32-33	35.9287457295252	41.0	38.0	41.0	23.0	41.0
34-35	35.77796794946032	41.0	38.0	41.0	20.0	41.0
36-37	35.68633586797145	40.0	38.0	41.0	18.5	41.0
38-39	35.56994986971832	40.0	38.0	41.0	18.0	41.0
40-41	35.444782867485316	40.0	37.5	41.0	16.0	41.0
42-43	35.312981609814216	40.0	37.0	41.0	15.0	41.0
44-45	35.27026965194793	40.0	37.0	41.0	14.5	41.0
46-47	35.27175821533319	40.0	37.0	41.0	10.5	41.0
48-49	35.19304171446622	40.0	37.0	41.0	10.0	41.0
50-51	34.70008284899416	39.5	36.5	40.5	8.5	41.0
52-53	34.77763568112621	40.0	36.5	41.0	7.5	41.0
54-55	34.95413873009373	40.0	37.0	41.0	7.0	41.0
56-57	34.84678248255562	40.0	36.5	41.0	2.0	41.0
58-59	34.68137155293354	40.0	36.0	41.0	2.0	41.0
60-61	34.5315231769394	40.0	36.0	41.0	2.0	41.0
62-63	34.29873148606507	40.0	35.0	41.0	2.0	41.0
64-65	33.97107070554355	39.0	35.0	41.0	2.0	41.0
66-67	33.620723932510245	39.0	35.0	41.0	2.0	41.0
68-69	33.29143871775119	38.5	35.0	41.0	2.0	41.0
70-71	32.902276077493	37.5	34.0	40.0	2.0	41.0
72-73	32.51026625115816	37.0	34.0	40.0	2.0	41.0
74-75	32.032970486709786	36.5	34.0	39.0	2.0	41.0
76-77	31.564591721938996	36.0	33.0	39.0	2.0	41.0
78-79	31.110837234896366	35.5	32.5	38.5	2.0	40.0
80-81	30.65708613428628	35.0	32.0	37.0	2.0	39.0
82-83	30.203333902264404	35.0	31.5	37.0	2.0	39.0
84-85	29.73537551549657	35.0	31.0	36.0	2.0	38.5
86-87	29.25313037844882	35.0	30.5	36.0	2.0	37.0
88-89	28.78186450046929	35.0	30.0	35.5	2.0	37.0
90-91	28.341465455742892	34.5	29.0	35.0	2.0	36.5
92-93	27.820397210434777	34.0	29.0	35.0	2.0	36.0
94-95	27.242193824323707	34.0	26.5	35.0	2.0	36.0
96-97	26.58452950739907	34.0	25.5	35.0	2.0	35.0
98-99	25.830946790514798	33.5	22.0	35.0	2.0	35.0
100-101	24.116942918152596	31.5	10.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3826456939571443
1101	2	0.3607802930122972
1101	3	0.3548995683243774
1101	4	0.33984041873198123
1101	5	0.33051766501132107
1101	6	0.27388714792960656
1101	7	0.24913038129108145
1101	8	0.2529005911175304
1101	9	0.3188123700941645
1101	10-11	0.3542491916132562
1101	12-13	0.3508485788427791
1101	14-15	0.4506178186233072
1101	16-17	0.46189750544132124
1101	18-19	0.46153029458825046
1101	20-21	0.4642182486476685
1101	22-23	0.437093749075693
1101	24-25	0.4431388418655544
1101	26-27	0.4410135469035481
1101	28-29	0.45747425951525145
1101	30-31	0.4641270563961726
1101	32-33	0.4682754442277002
1101	34-35	0.47064916678312585
1101	36-37	0.4675547266127893
1101	38-39	0.4235098996652553
1101	40-41	0.4034889131920849
1101	42-43	0.45223171807627693
1101	44-45	0.4501508596377022
1101	46-47	0.513113945645209
1101	48-49	0.5090454683338095
1101	50-51	0.43599949486424094
1101	52-53	0.47004421514987627
1101	54-55	0.49885700562195723
1101	56-57	0.4929030640367884
1101	58-59	0.5066375859128982
1101	60-61	0.5126726824279331
1101	62-63	0.5222442542705323
1101	64-65	0.5348436488204484
1101	66-67	0.5595316386851579
1101	68-69	0.5411063327831513
1101	70-71	0.5290363591933556
1101	72-73	0.5007136707014368
1101	74-75	0.5686580354401443
1101	76-77	0.6044988466338488
1101	78-79	0.5444540413551451
1101	80-81	0.5610972168869388
1101	82-83	0.5286163144250011
1101	84-85	0.5651468199720959
1101	86-87	0.5267514986543098
1101	88-89	0.5428489582838871
1101	90-91	0.560269238223924
1101	92-93	0.5717584174709849
1101	94-95	0.5780433577754103
1101	96-97	0.6096041629647964
1101	98-99	0.5844832230587009
1101	100-101	0.4956419080586727
1102	1	-0.025155354228630245
1102	2	0.06139797342415676
1102	3	0.05494951757192723
1102	4	0.04118911619777066
1102	5	0.05338583653269069
1102	6	0.009760582415175634
1102	7	0.019333179551047408
1102	8	0.017574598598010027
1102	9	0.021481430106945254
1102	10-11	-0.016620572777405584
1102	12-13	0.02977403190605088
1102	14-15	0.03438006163734286
1102	16-17	-0.01500360556268987
1102	18-19	6.950871062656461E-4
1102	20-21	0.029134057899078414
1102	22-23	0.015701053948525612
1102	24-25	-0.03004213583258064
1102	26-27	-0.07585769672989073
1102	28-29	-0.12430355608429977
1102	30-31	-0.09517052669641402
1102	32-33	-0.029715317622368786
1102	34-35	-0.05063243711745713
1102	36-37	-0.08415759071664297
1102	38-39	-0.07880387116011889
1102	40-41	-0.11410554935129369
1102	42-43	-0.11153619872292353
1102	44-45	-0.11891377400520753
1102	46-47	-0.10290848907400374
1102	48-49	0.1418922516175698
1102	50-51	0.10282072975575574
1102	52-53	-0.06971319010092003
1102	54-55	-0.05019407862688041
1102	56-57	-0.08332928830628106
1102	58-59	-0.14233047946427746
1102	60-61	-0.12084832718321081
1102	62-63	-0.08325613537822818
1102	64-65	-0.07093894776709675
1102	66-67	-0.07159054075638949
1102	68-69	-0.07762787200874754
1102	70-71	-0.09859715973418304
1102	72-73	-0.09840699242543138
1102	74-75	-0.038879557928666486
1102	76-77	-0.03381638862895997
1102	78-79	-0.08529860614195073
1102	80-81	-0.06224205385645121
1102	82-83	-0.039400034399367456
1102	84-85	0.02862630896310847
1102	86-87	0.026860551254980436
1102	88-89	0.00917618555720523
1102	90-91	0.02000189588413548
1102	92-93	0.03144536281307353
1102	94-95	0.02776528142730328
1102	96-97	0.09368721007724545
1102	98-99	0.16777538415197668
1102	100-101	0.12235312773955087
1103	1	0.2255235105850275
1103	2	0.2636320393163416
1103	3	0.2670443069710444
1103	4	0.2355438944309114
1103	5	0.22994143434596026
1103	6	0.21674116485836947
1103	7	0.17507764005043214
1103	8	0.17807292915940565
1103	9	0.2145225689002217
1103	10-11	0.2169549574047025
1103	12-13	0.23782812367865347
1103	14-15	0.2610019625023128
1103	16-17	0.3118743550655765
1103	18-19	0.3282445887890688
1103	20-21	0.3329693394483684
1103	22-23	0.29224794628067485
1103	24-25	0.30810790757726636
1103	26-27	0.33131295408095696
1103	28-29	0.31140869478675626
1103	30-31	0.31680420169089274
1103	32-33	0.30698660171186276
1103	34-35	0.32164177595205246
1103	36-37	0.3196321819524286
1103	38-39	0.29155111684967494
1103	40-41	0.34702154293712795
1103	42-43	0.36591020261800367
1103	44-45	0.3840625919306291
1103	46-47	0.36718594678174554
1103	48-49	0.3824719592129924
1103	50-51	0.3779851351061083
1103	52-53	0.39977976071902077
1103	54-55	0.4327471116067869
1103	56-57	0.4331842335370908
1103	58-59	0.45251856718913075
1103	60-61	0.450927955073233
1103	62-63	0.4697823028347372
1103	64-65	0.5183244552466775
1103	66-67	0.5267323409452729
1103	68-69	0.5553038057128248
1103	70-71	0.5268050540457949
1103	72-73	0.5299682804929304
1103	74-75	0.6053385449378439
1103	76-77	0.6292494434600435
1103	78-79	0.6101023520522872
1103	80-81	0.6042684012824964
1103	82-83	0.6195878425828596
1103	84-85	0.705341349560431
1103	86-87	0.6995405343961814
1103	88-89	0.7623555049081077
1103	90-91	0.8606572368350669
1103	92-93	0.9405580429717126
1103	94-95	0.9742165427831253
1103	96-97	1.1427406026872085
1103	98-99	1.2481169336260542
1103	100-101	1.249147416740911
1104	1	0.19328686379825655
1104	2	0.18299382450668134
1104	3	0.17860586568877324
1104	4	0.20280541870193503
1104	5	0.17675256654502647
1104	6	0.1561367147498558
1104	7	0.1434429911105113
1104	8	0.1406642366129347
1104	9	0.17677315364682755
1104	10-11	0.1783761550059424
1104	12-13	0.1935632333202264
1104	14-15	0.18207464958977226
1104	16-17	0.24596214458158983
1104	18-19	0.23946095269425172
1104	20-21	0.2482774204988658
1104	22-23	0.2013137514013792
1104	24-25	0.21170995731125686
1104	26-27	0.22481647988521303
1104	28-29	0.23313810839077576
1104	30-31	0.2466777747861144
1104	32-33	0.24423974884869182
1104	34-35	0.22101308847432932
1104	36-37	0.22716359595293056
1104	38-39	0.22541910118618347
1104	40-41	0.22196036643072858
1104	42-43	0.1965950620141541
1104	44-45	0.2426637794916502
1104	46-47	0.2781363830722725
1104	48-49	0.2972482314189193
1104	50-51	0.24753787638451996
1104	52-53	0.20747672782885473
1104	54-55	0.24523320121355852
1104	56-57	0.2807734227622163
1104	58-59	0.28333790073259024
1104	60-61	0.28536625825135076
1104	62-63	0.2815108296833202
1104	64-65	0.3212118054193809
1104	66-67	0.31987316045147907
1104	68-69	0.2999324814533466
1104	70-71	0.32291693486708084
1104	72-73	0.32382366482928404
1104	74-75	0.38095280282928456
1104	76-77	0.4250633409398823
1104	78-79	0.4134791652562839
1104	80-81	0.4137971649117169
1104	82-83	0.4049013566984918
1104	84-85	0.464256058313989
1104	86-87	0.4492235056706768
1104	88-89	0.5056446007333939
1104	90-91	0.597304435251683
1104	92-93	0.6713692840022851
1104	94-95	0.6698849184788962
1104	96-97	0.7656167505939955
1104	98-99	0.8183262567954372
1104	100-101	0.801940538701146
1105	1	0.10587278493832031
1105	2	0.10532126379853679
1105	3	0.11704804416245196
1105	4	0.12469967885598976
1105	5	0.10582341989663746
1105	6	0.06939524198565294
1105	7	0.09163691152674147
1105	8	0.08423858146360885
1105	9	0.13390774300432895
1105	10-11	0.09459761802206401
1105	12-13	0.08953895921374055
1105	14-15	0.09083950156119869
1105	16-17	0.11247828987726649
1105	18-19	0.08115743539617881
1105	20-21	0.0859246516775869
1105	22-23	0.09278555355609086
1105	24-25	0.06075630695447387
1105	26-27	0.06964924616223556
1105	28-29	0.06781424322186069
1105	30-31	0.06473570736432777
1105	32-33	0.0599983279581906
1105	34-35	0.06811763275099736
1105	36-37	0.06599190661100351
1105	38-39	0.04116968396978393
1105	40-41	0.09311321254496363
1105	42-43	0.08898676206037948
1105	44-45	0.08860108803647648
1105	46-47	0.09352883175365179
1105	48-49	0.0862471148089412
1105	50-51	0.06269364155667745
1105	52-53	0.10673661438166704
1105	54-55	0.08029244481041076
1105	56-57	0.05582561464915159
1105	58-59	0.07264361010861364
1105	60-61	0.08825063351779505
1105	62-63	0.1220570015888427
1105	64-65	0.10682922935213668
1105	66-67	0.09913148574248964
1105	68-69	0.07478548980125765
1105	70-71	0.10766164969795966
1105	72-73	0.13277041502256992
1105	74-75	0.19591245188445328
1105	76-77	0.22522643935831965
1105	78-79	0.20697379140629124
1105	80-81	0.22669755154208815
1105	82-83	0.2299889502528707
1105	84-85	0.2959208372391977
1105	86-87	0.3514730286262626
1105	88-89	0.3598193802614169
1105	90-91	0.40075491175229416
1105	92-93	0.49234304070768076
1105	94-95	0.5214024195352458
1105	96-97	0.5626774003555894
1105	98-99	0.6246946771343396
1105	100-101	0.6006780949740538
1106	1	-0.05797885138957781
1106	2	-0.01786346642966663
1106	3	-0.038568612867933894
1106	4	-0.0421002734403757
1106	5	-0.04613997872046838
1106	6	-0.06385244981041893
1106	7	-0.03547398975374705
1106	8	-0.02823399177449204
1106	9	-0.028904375386176184
1106	10-11	-0.08666395378851632
1106	12-13	-0.05900536349256669
1106	14-15	-0.09829010537910676
1106	16-17	-0.10813531016879807
1106	18-19	-0.10886896252834077
1106	20-21	-0.09700725871399385
1106	22-23	-0.10513901021070637
1106	24-25	-0.1253365283099086
1106	26-27	-0.09462930572322392
1106	28-29	-0.09359503474895803
1106	30-31	-0.10729198993551137
1106	32-33	-0.1156171816422642
1106	34-35	-0.11687420705681006
1106	36-37	-0.11140127249004905
1106	38-39	-0.11743956057204485
1106	40-41	-0.10653873332307029
1106	42-43	-0.09818541465887876
1106	44-45	-0.10515014539535628
1106	46-47	-0.11131065565719211
1106	48-49	-0.12095915683514136
1106	50-51	-0.27372541212134394
1106	52-53	-0.2997391236020519
1106	54-55	-0.22388380109980233
1106	56-57	-0.2802410618533244
1106	58-59	-0.26025944535782486
1106	60-61	-0.22678413764302974
1106	62-63	-0.19620866814335614
1106	64-65	-0.2148088080374677
1106	66-67	-0.18908836762859238
1106	68-69	-0.1806271496394345
1106	70-71	-0.18886096875756664
1106	72-73	-0.17006755359788883
1106	74-75	-0.1256074668667111
1106	76-77	-0.08801700734192508
1106	78-79	-0.050195420992785955
1106	80-81	-0.05778291090327059
1106	82-83	-0.05793262994287929
1106	84-85	0.015630280063628987
1106	86-87	0.04513267229848239
1106	88-89	-0.007637245094510803
1106	90-91	0.06169876745737568
1106	92-93	0.14890807959421437
1106	94-95	0.19171322398612745
1106	96-97	0.2255080019813427
1106	98-99	0.2888984172350568
1106	100-101	0.2286419965074593
1107	1	-0.3163176136723962
1107	2	-0.3326830331615511
1107	3	-0.3362110016320017
1107	4	-0.24409696665511404
1107	5	-0.2185735361839889
1107	6	-0.2801066911781902
1107	7	-0.2156437192843157
1107	8	-0.20926706159274033
1107	9	-0.27226999277361585
1107	10-11	-0.2925555104711961
1107	12-13	-0.325614567856114
1107	14-15	-0.4791711106019605
1107	16-17	-0.5388259510157667
1107	18-19	-0.450232826051419
1107	20-21	-0.50791524541863
1107	22-23	-0.5267232990082036
1107	24-25	-0.5026190596748492
1107	26-27	-0.502927501831671
1107	28-29	-0.5786037070725172
1107	30-31	-0.5693290554967021
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2308	56-57	-1.3380866020916997
2308	58-59	-1.2363699806182922
2308	60-61	-1.2606732653342902
2308	62-63	-1.2145027410540976
2308	64-65	-1.322582296622592
2308	66-67	-1.2960966578710895
2308	68-69	-1.2488181030120842
2308	70-71	-1.2960182359025048
2308	72-73	-1.3065310395028753
2308	74-75	-1.3093224169557836
2308	76-77	-1.2908728108360101
2308	78-79	-1.4679576685947389
2308	80-81	-1.6591691114771088
2308	82-83	-1.7183968727673182
2308	84-85	-1.9265552365398158
2308	86-87	-2.137517986090188
2308	88-89	-2.3066542361764952
2308	90-91	-2.493804067022033
2308	92-93	-2.7825644699787198
2308	94-95	-3.053236277763258
2308	96-97	-3.536395102382052
2308	98-99	-3.968691871924829
2308	100-101	-4.327543540420805
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	422836.0
3	112805.0
4	92653.0
5	101725.0
6	153129.0
7	273972.0
8	494999.0
9	811836.0
10	1064327.0
11	940531.0
12	624559.0
13	459457.0
14	375526.0
15	330438.0
16	303078.0
17	291487.0
18	296126.0
19	291266.0
20	308340.0
21	315485.0
22	339148.0
23	371696.0
24	432593.0
25	475971.0
26	435188.0
27	486120.0
28	543343.0
29	623135.0
30	730003.0
31	880781.0
32	1098598.0
33	1403569.0
34	1903892.0
35	2752077.0
36	4448590.0
37	1.0205613E7
38	1.987875E7
39	8984831.0
40	20760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.684927200172417	16.735582669021948	9.208241706668636	43.371248424137
2	14.581299863926024	17.594379889372178	54.07135051522963	13.752969731472161
3	18.918524944875053	21.28987655308886	38.39617086628516	21.395427635750927
4	19.599031506311665	27.157990026857014	35.05822929851613	18.18474916831519
5	20.423545686315016	31.130973081593194	34.61587884958405	13.829602382507735
6	15.059484555073489	34.72295066110716	35.86877575855969	14.348789025259665
7	12.704031731561264	19.1563832064004	53.86103420076699	14.278550861271347
8	13.226273986698448	22.795891393307784	46.5604565251919	17.417378094801865
9	14.599548207488503	22.09964407859921	45.552988240378426	17.74781947353386
10-11	16.947308896731265	32.76503666326555	35.78156527078047	14.506089169222717
12-13	16.53084603799728	26.226077151103173	41.11576548871937	16.12731132218018
14-15	17.152168892650668	28.32771152616446	39.062719015996024	15.45740056518885
16-17	18.440688651843303	28.714707583108257	37.12697905891706	15.717624706131387
18-19	18.664451538004876	28.64869107852864	36.25519405444616	16.43166332902033
20-21	19.040789535809033	29.331128095336318	34.97913138183514	16.64895098701951
22-23	18.721261760579047	29.85827743053349	35.243637899478124	16.17682290940934
24-25	18.305209620799808	30.226855522174983	34.02782759548957	17.440107261535644
26-27	18.12756072948833	30.889345198674008	33.747351812367405	17.235742259470253
28-29	18.990443405676654	30.27491641384995	32.958700107524194	17.775940072949208
30-31	18.807230406566422	30.86534038270605	32.872010224832536	17.455418985894994
32-33	18.146039377999873	30.302325898404398	33.19713525482082	18.35449946877491
34-35	18.6699078036519	31.11840205903328	32.123891573337815	18.08779856397701
36-37	18.465676936967558	30.927092444979053	32.59905031602134	18.00818030203205
38-39	18.488183402923344	30.089250025984448	33.033990350296975	18.388576220795237
40-41	18.96671200352619	30.354599584194595	32.97262733008341	17.706061082195802
42-43	18.67395237719782	30.65548619206158	33.04954889570632	17.62101253503427
44-45	18.84694907429448	29.772694354458018	33.04889432440992	18.33146224683758
46-47	18.06393068385671	31.351041244597216	32.252160154277405	18.332867917268665
48-49	17.629834521031967	30.75419338937519	32.84898863727103	18.766983452321814
50-51	17.93119864898836	30.66494772423797	33.319877901339716	18.08397572543395
52-53	18.369849597853168	30.863520644748483	32.647350514497845	18.119279242900507
54-55	18.548810511192613	31.60008359580865	31.59866306649382	18.252442826504918
56-57	19.325496833956908	31.00562849242936	31.80716554246194	17.86170913115179
58-59	18.20243516044167	32.334984195238015	31.624470640754083	17.838110003566236
60-61	18.61237447281719	31.96659498280509	31.25406738406829	18.16696316030943
62-63	18.385917107964126	32.893973919424376	30.80093836034896	17.919170612262533
64-65	18.51840085649167	31.61343320012055	31.317496781950794	18.550669161436986
66-67	18.743498903744058	32.25102440665163	30.88913919740126	18.116337492203055
68-69	19.157622586247	31.555826211056498	31.239780473978602	18.0467707287179
70-71	18.263424141136735	31.731741306593346	31.03771551040285	18.96711904186707
72-73	18.476391608795993	31.783953446132664	30.960971285014544	18.778683660056803
74-75	19.097922620296927	31.166528422373613	30.91535179783796	18.820197159491507
76-77	18.404416932801738	31.774862352932963	30.931294853678125	18.88942586058717
78-79	18.62620527392272	32.51298595793893	30.48630024823678	18.37450851990156
80-81	18.534804522353205	32.7006953578423	30.845644859829136	17.91885525997536
82-83	19.063086080325697	32.00039741682809	30.76808281309781	18.1684336897484
84-85	18.874351007482886	32.092662062572344	30.753545518359655	18.27944141158511
86-87	18.77829310451296	32.5173121600481	30.66609552132587	18.038299214113067
88-89	19.124424193370324	31.92366210151502	30.716989081116434	18.234924623998218
90-91	18.396394665963513	32.62806896092255	30.458600318101357	18.51693605501258
92-93	19.52087458227959	31.82799821048813	30.680253465313516	17.970873741918766
94-95	19.554531534262207	32.41470002206877	29.622630287723716	18.408138155945306
96-97	18.896210069487964	32.884250395726966	30.402174570731155	17.817364964053915
98-99	20.053004165878658	31.87992339478502	29.769779946287866	18.29729249304846
100-101	20.068998488487523	32.662687423534884	29.03431825554181	18.233995832435777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32528.0
1	39722.0
2	42930.0
3	44726.0
4	58327.0
5	75346.5
6	91346.5
7	106720.5
8	124669.5
9	142331.0
10	158971.0
11	175420.5
12	191811.5
13	206972.5
14	226530.0
15	245968.0
16	261102.5
17	282483.5
18	314133.5
19	351429.5
20	403907.0
21	475907.0
22	558156.0
23	634743.5
24	743882.0
25	933085.5
26	1119272.0
27	1346695.5
28	1568053.0
29	1755971.0
30	1954017.0
31	2057774.0
32	2171471.5
33	2369668.0
34	2567569.0
35	2705506.0
36	2880989.5
37	3009579.5
38	3096061.0
39	3040253.5
40	3005350.0
41	3043277.0
42	2592032.0
43	2071588.5
44	1806616.0
45	1627710.5
46	1536760.0
47	1448165.5
48	1361940.5
49	1251282.0
50	1057473.5
51	853423.0
52	717406.5
53	606649.5
54	501203.0
55	423532.0
56	367501.5
57	306256.0
58	245610.5
59	194476.5
60	143933.0
61	101343.5
62	76445.0
63	58257.5
64	41677.5
65	28635.5
66	19091.0
67	12861.5
68	8442.0
69	5144.5
70	3296.5
71	2351.0
72	1818.5
73	1550.5
74	1319.0
75	1058.5
76	854.5
77	694.0
78	598.5
79	464.5
80	332.5
81	246.5
82	192.0
83	146.0
84	101.5
85	80.0
86	59.0
87	48.0
88	40.5
89	27.0
90	25.0
91	17.5
92	15.5
93	15.0
94	10.5
95	6.5
96	4.5
97	4.0
98	2.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1275920390620156
2	0.15271562317233103
3	0.14832886654557803
4	0.07594660192015719
5	0.07576401546504154
6	0.1458569268455507
7	0.14587409309346758
8	0.15267816954051244
9	0.1765689049367991
10-11	0.16031793014750972
12-13	0.17884109193379388
14-15	0.19484861811626242
16-17	0.2260272060372508
18-19	0.24686703100831436
20-21	0.2641659272045282
22-23	0.2670662428184807
24-25	0.2694063145231467
26-27	0.2431333720863981
28-29	0.23102258418105595
30-31	0.23312388898287842
32-33	0.2849441097398903
34-35	0.28836565506331824
36-37	0.294833429107992
38-39	0.2890889783278149
40-41	0.2972140755804614
42-43	0.3043170007980589
44-45	0.2991054839873005
46-47	0.2967607305786572
48-49	0.06337700702503726
50-51	0.022859199953282835
52-53	0.013428687574958959
54-55	0.02903436749937378
56-57	0.03714698020808083
58-59	0.03352490189762415
60-61	0.02895867995174037
62-63	0.030952305562084368
64-65	0.03521889845341938
66-67	0.04447150608060493
68-69	0.04008709030584058
70-71	0.038542127993323516
72-73	0.04022988227714898
74-75	0.031098998953373865
76-77	0.03507220506212988
78-79	0.02811753380381441
80-81	0.04207369336021859
82-83	0.055624885522584204
84-85	0.060728723141864074
86-87	0.05069973293843888
88-89	0.046405049823239924
90-91	0.05048749569146685
92-93	0.04582529881404791
94-95	0.042415457750563275
96-97	0.04537273409623988
98-99	0.03551618665597948
100-101	0.03981165005517466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6.4079233E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.688239028773484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.38462448139913	18.567937208960323
2	8.992484598825646	4.260322492790923
3	3.523507635555962	2.503970732722743
4	1.9341587635617319	1.8326726044338912
5	1.2071889994914484	1.4298090786429674
6	0.8395196000948922	1.1932044573832878
7	0.6585815184480245	1.092044550025058
8	0.45939175444013375	0.87057453496204
9	0.38586146131920435	0.8226340674949033
>10	2.8479594429587567	13.614015102449414
>50	0.38648390387699	6.407290193556977
>100	0.3135214997620696	14.937839501175054
>500	0.036721031422848445	5.978619042769212
>1k	0.025519178752712715	11.837835392401455
>5k	0.0027346792005890413	4.384913376733809
>10k+	0.0017414508899519822	10.266317663497745
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	288944	0.45091675800801173	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	199489	0.3113161482441589	No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	189294	0.29540615756121796	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	168992	0.263723506178671	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	146634	0.22883232700366438	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	127815	0.19946399795390807	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	126452	0.19733694378021036	Illumina Single End PCR Primer 1 (100% over 50bp)
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	124894	0.19490557884798654	No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	99095	0.15464448521098872	No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC	88757	0.1385113333051287	No Hit
GCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAA	85473	0.13338642801795084	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84946	0.13256400868593418	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	84215	0.13142323348345947	No Hit
GTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATC	73766	0.1151168585304384	No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT	70245	0.10962209862905195	No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT	67882	0.10593447646291272	No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC	66016	0.1030224565890169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.23768698979277733	0.0	0.0	2.9650791856388168E-5	0.0
2	0.23851877253274864	0.0	0.0	3.901419981103706E-5	1.5605679924414825E-6
3	0.2386904350119172	0.0	0.0	5.9301583712776336E-5	1.4045111931973342E-5
4	0.2744492899907213	0.0	0.0	6.866499166742524E-5	1.5605679924414826E-5
5	0.27544025066592165	0.0	0.0	7.802839962207413E-5	1.716624791685631E-5
6	0.2761549908064599	0.0	0.0	9.519464753893043E-5	1.716624791685631E-5
7	0.27895777092088475	0.0	0.0	1.2796657538020157E-4	1.716624791685631E-5
8	0.3195497049722802	0.0	0.0	1.3576941534240898E-4	1.716624791685631E-5
9	0.32013647853743815	0.0	0.0	1.701019111761216E-4	1.716624791685631E-5
10-11	0.3297651830508021	7.802839962207413E-7	0.0	1.833667391118742E-4	1.716624791685631E-5
12-13	0.33484951357017645	1.5605679924414825E-6	0.0	2.0365412301361345E-4	1.716624791685631E-5
14-15	0.3377669954320458	1.5605679924414825E-6	0.0	2.325246308737809E-4	1.716624791685631E-5
16-17	0.34025610137998996	2.3408519886622237E-6	0.0	2.6217542273016903E-4	1.716624791685631E-5
18-19	0.3426461112604141	3.121135984882965E-6	0.0	3.2381785843160765E-4	1.716624791685631E-5
20-21	0.34536384041925094	3.121135984882965E-6	0.0	3.8858143011792916E-4	1.716624791685631E-5
22-23	0.3483983648805534	3.121135984882965E-6	0.0	4.2447449394408327E-4	1.716624791685631E-5
24-25	0.35109892779147345	3.121135984882965E-6	0.0	4.962606215963914E-4	1.716624791685631E-5
26-27	0.3539594489216187	3.9014199811037065E-6	0.0	6.952330406326806E-4	1.716624791685631E-5
28-29	0.35727253476957194	4.681703977324447E-6	0.0	8.114953560695709E-4	1.872681590929779E-5
30-31	0.36103194306336345	7.802839962207413E-6	0.0	8.770392117521132E-4	1.872681590929779E-5
32-33	0.3647554582933288	7.802839962207413E-6	0.0	8.903040396878658E-4	2.0287383901739273E-5
34-35	0.3689050085852307	7.802839962207413E-6	0.0	9.246365355215784E-4	2.26282358904015E-5
36-37	0.37297887132949925	7.802839962207413E-6	0.0	9.95642379177666E-4	2.8090223863946685E-5
38-39	0.3774272703919537	7.802839962207413E-6	0.0	0.0010159297630794053	4.4476187784582256E-5
40-41	0.3825904095949463	7.802839962207413E-6	0.0	0.0010627468028526496	4.837760776568596E-5
42-43	0.3879002421892285	9.363407954648895E-6	0.0	0.0011712062783273327	5.071845975434818E-5
44-45	0.39400830531164444	1.0143691950869637E-5	1.5605679924414825E-6	0.0012320684300325506	5.1498743750568925E-5
46-47	0.40776861358499716	1.0923975947090378E-5	1.5605679924414825E-6	0.0013366264855261298	5.1498743750568925E-5
48-49	0.4161886581882152	1.32648279357526E-5	1.5605679924414825E-6	0.0013865646612842572	5.1498743750568925E-5
50-51	0.4249621714417212	1.4045111931973342E-5	1.5605679924414825E-6	0.0014185563051293077	5.1498743750568925E-5
52-53	0.43475395531029526	1.4825395928194084E-5	1.5605679924414825E-6	0.0014404042570234884	5.1498743750568925E-5
54-55	0.4457988752767999	1.5605679924414826E-5	1.5605679924414825E-6	0.0014692747648836558	5.1498743750568925E-5
56-57	0.45756399737181624	1.6385963920635568E-5	1.5605679924414825E-6	0.0014919030007740574	5.305931174301041E-5
58-59	0.47097317784686965	1.716624791685631E-5	1.5605679924414825E-6	0.0015379397565510812	5.305931174301041E-5
60-61	0.4861372170294236	1.7946531913077048E-5	1.5605679924414825E-6	0.0015683708324036902	5.305931174301041E-5
62-63	0.5031107347992134	1.950709990551853E-5	1.5605679924414825E-6	0.0016229907121391419	5.305931174301041E-5
64-65	0.5216549642533954	2.26282358904015E-5	1.5605679924414825E-6	0.0016698077519123862	5.383959573923115E-5
66-67	0.5420000891708551	2.3408519886622237E-5	1.5605679924414825E-6	0.0017181853596780722	5.618044772789337E-5
68-69	0.5643653693545302	2.418880388284298E-5	1.5605679924414825E-6	0.0017486164355306811	5.774101572033485E-5
70-71	0.5914388831714013	2.7309939867725943E-5	1.5605679924414825E-6	0.0017759263753984072	5.774101572033485E-5
72-73	0.6206402314459663	2.8090223863946685E-5	2.3408519886622237E-6	0.0018032363152661332	5.774101572033485E-5
74-75	0.6542455025952012	2.8090223863946685E-5	3.121135984882965E-6	0.0018625378989789096	5.9301583712776336E-5
76-77	0.6949544792460297	2.8090223863946685E-5	3.121135984882965E-6	0.002034980662143693	5.9301583712776336E-5
78-79	0.7424285181440919	2.9650791856388168E-5	3.121135984882965E-6	0.002107547073792222	5.9301583712776336E-5
80-81	0.797943383623209	2.9650791856388168E-5	3.121135984882965E-6	0.0021980600173538284	5.9301583712776336E-5
82-83	0.8630619845278111	3.043107585260891E-5	3.121135984882965E-6	0.0022729672809910194	5.9301583712776336E-5
84-85	0.9425698026067197	3.2771927841271135E-5	3.121135984882965E-6	0.002312761764798277	5.9301583712776336E-5
86-87	1.041086899401558	3.5893063826154096E-5	3.121135984882965E-6	0.002354897100594197	5.9301583712776336E-5
88-89	1.1575591112334318	3.745363181859558E-5	3.121135984882965E-6	0.0023837676084543643	5.9301583712776336E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	44985	0.0	62.792202	1
GCGTGTT	60590	0.0	46.440353	2
GTTTTTT	942555	0.0	44.58588	1
CGGCCTT	9215	0.0	40.89521	1
ACCACCG	6525	0.0	38.236084	1
GAGCGGC	7915	0.0	37.104485	9
CCACCGA	6960	0.0	36.529686	2
CGCCGTA	46545	0.0	35.80218	46-47
CCGTATC	48050	0.0	34.741467	48-49
GTCGCCG	48970	0.0	34.215675	44-45
CCCGAAA	12710	0.0	33.80006	1
ACCGAGA	7740	0.0	32.900795	4
CGTGTTT	87250	0.0	32.61156	3
CGGGCCT	7005	0.0	32.088364	1
ATCGGGA	6450	0.0	31.239748	2
CAAAACG	107085	0.0	30.899864	9
TCGGTGG	55270	0.0	30.448545	38-39
TCTCGGT	53795	0.0	30.243652	36-37
GGCGCCG	5835	0.0	29.55514	44-45
CGGCAAC	4415	0.0	28.631596	1
>>END_MODULE