FastQCFastQC Report
Fri 27 May 2016
SRR1233041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64079233
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2507060.3912437591130343TruSeq Adapter, Index 7 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT2186750.34125720574714125No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT2039920.31834338591412287No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1830900.285724393736111No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT1809940.28245344322395366No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC1332430.20793476101688044No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1312800.20487136604771783TruSeq Adapter, Index 7 (100% over 49bp)
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA1134770.17708857407828213No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA1055520.16472107273818337No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC1004070.15669195041707196No Hit
GTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATC737640.11511373739445353No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC711290.1110016407343702No Hit
GCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAA691950.10798350223698838No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT678450.10587673544719238No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671510.104793701260438No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT662500.10338762949924822No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC661530.1032362544039814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT460100.066.218331
GCGTGTT620050.049.008382
GTTTTTT9397150.046.9120451
CGGTGGT79300.044.8893361
CGGCCTT80300.036.6258471
CCCGAAA107700.036.5872231
CGGGCCT55000.036.514431
TATGCCG670200.033.98851446-47
CGTGTTT916750.033.551153
TGCCGTC674600.033.52710348-49
CCGTCTT690950.032.78539350-51
CTCGTAT702500.031.86819642-43
CAAAACG944100.029.5607389
ATCTCGT765900.028.87971940-41
CGTCTGA857800.027.0311216-17
TCAACGG145800.027.02160816-17
TCGTCTA125050.026.70118544-45
CCGAAAC146600.026.3720132
TCACCAG913800.025.91508530-31
CCAGTCA869050.025.88871226-27
Produced by FastQC (version 0.11.5)