FastQCFastQC Report
Fri 27 May 2016
SRR1233040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81607113
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT2209900.2707974732545679No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT2123490.26020893546375057No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT1997970.2448279232718354No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1750010.21444331696919605Illumina Single End PCR Primer 1 (100% over 50bp)
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT1434970.17583883895022728No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1236530.1515223311477763No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT1070480.13117484011473363No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1046290.1282106377173274No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1037500.12713352572587638No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC846020.10366988475624668No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC838750.10277903103863018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT731650.068.400081
GCGTGTT992050.050.4852
GTTTTTT15359900.043.2598041
ACCACCG79750.042.311581
CCACCGA86450.039.5826842
CCCGAAA228600.035.9667781
CGCCGTA447300.034.1132846-47
CCGAAAC240650.033.6133232
CGTGTTT1514600.033.4612433
GTCGCCG476050.032.7950244-45
CGGCAAC64900.032.3675041
CCGTATC474200.032.326948-49
ACCGAGA105150.032.240644
TCGTCTA194700.031.49479944-45
CGGCCTT75050.031.0929851
CGAAACC266700.030.50073
CGAGCTA208550.030.20939812-13
CCCAGAT407500.029.8918381
GTGTTTT3636800.029.472471
TCGGTGG563100.028.91678638-39
Produced by FastQC (version 0.11.5)