##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233040_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81607113 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.931873193455576 33.0 31.0 34.0 27.0 34.0 2 31.096872646383165 33.0 31.0 34.0 27.0 34.0 3 31.049337929648363 34.0 31.0 34.0 27.0 34.0 4 34.329178548443444 37.0 35.0 37.0 30.0 37.0 5 34.278367438387384 37.0 35.0 37.0 30.0 37.0 6 34.2880204327287 37.0 35.0 37.0 30.0 37.0 7 34.48995557531854 37.0 35.0 37.0 31.0 37.0 8 34.466114577536885 37.0 35.0 37.0 30.0 37.0 9 35.94928333024108 39.0 37.0 39.0 30.0 39.0 10-11 35.815432203808015 39.0 37.0 39.0 30.0 39.0 12-13 35.730504809059966 39.0 37.0 39.0 30.0 39.0 14-15 36.86073508077659 40.0 37.0 41.0 29.5 41.0 16-17 36.55387160920642 40.0 37.0 41.0 28.0 41.0 18-19 36.06708405430297 40.0 36.0 41.0 27.0 41.0 20-21 35.647782981858455 40.0 35.0 41.0 26.5 41.0 22-23 35.017014656553286 39.0 35.0 41.0 21.5 41.0 24-25 34.603783006023015 39.0 35.0 41.0 18.0 41.0 26-27 34.11779152143269 39.0 34.0 41.0 11.5 41.0 28-29 33.59435068484778 39.0 34.0 41.0 7.5 41.0 30-31 33.32290786956279 39.0 34.0 41.0 2.0 41.0 32-33 33.142496849508696 39.0 33.0 41.0 2.0 41.0 34-35 32.95620688725994 39.0 33.0 41.0 2.0 41.0 36-37 32.75673842303428 39.0 33.0 41.0 2.0 41.0 38-39 32.65289404368465 39.0 33.0 41.0 2.0 41.0 40-41 32.51288738152029 39.0 32.5 41.0 2.0 41.0 42-43 32.39544490441661 39.0 32.0 41.0 2.0 41.0 44-45 32.28581835752479 39.0 32.0 41.0 2.0 41.0 46-47 32.16825337884456 39.0 31.0 41.0 2.0 41.0 48-49 32.111850942454 39.0 31.0 41.0 2.0 41.0 50-51 31.573177659158215 38.5 30.5 40.5 2.0 41.0 52-53 31.66369423581986 38.5 31.0 40.5 2.0 41.0 54-55 31.83229358940807 39.0 31.0 41.0 2.0 41.0 56-57 31.674954798364205 39.0 31.0 41.0 2.0 41.0 58-59 31.521037345114756 39.0 31.0 41.0 2.0 41.0 60-61 31.357848561558598 38.0 30.5 41.0 2.0 41.0 62-63 30.895786492287748 38.0 29.0 41.0 2.0 41.0 64-65 30.794535661370595 38.0 29.0 40.0 2.0 41.0 66-67 30.56449712906766 37.5 29.0 40.0 2.0 41.0 68-69 30.272215970928904 37.0 28.5 40.0 2.0 41.0 70-71 29.89131433922923 36.5 28.0 39.5 2.0 41.0 72-73 29.527111644054852 36.0 28.0 39.0 2.0 41.0 74-75 29.038654271472637 35.5 26.5 39.0 2.0 41.0 76-77 28.62837784740651 35.0 26.0 38.5 2.0 40.0 78-79 28.174003545499765 35.0 26.0 37.0 2.0 39.5 80-81 27.618347833233607 35.0 24.0 37.0 2.0 39.0 82-83 27.22463693207723 34.0 23.5 36.0 2.0 39.0 84-85 26.62258519058259 34.0 20.5 36.0 2.0 37.5 86-87 26.1779810909375 34.0 20.0 35.0 2.0 37.0 88-89 25.804024160246914 34.0 18.0 35.0 2.0 37.0 90-91 25.371892318994302 34.0 10.0 35.0 2.0 36.0 92-93 24.798519174425394 33.0 2.0 35.0 2.0 36.0 94-95 24.136157965789085 32.5 2.0 35.0 2.0 35.5 96-97 23.436642569379952 32.0 2.0 35.0 2.0 35.0 98-99 22.61270374801765 31.0 2.0 34.5 2.0 35.0 100-101 21.05521298125079 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9761725876517886 1101 2 1.0225397157062766 1101 3 1.046923198945919 1101 4 1.0751570880810561 1101 5 1.0723145734971027 1101 6 1.0528276852759433 1101 7 0.9217529968769469 1101 8 0.9056519141677271 1101 9 1.1383668579914357 1101 10-11 1.1524331035677804 1101 12-13 1.1750139899560352 1101 14-15 1.404477012640136 1101 16-17 1.422419238694829 1101 18-19 1.3265050284770084 1101 20-21 1.3934251006144365 1101 22-23 1.56508928713491 1101 24-25 1.4410167715059217 1101 26-27 1.36533434752252 1101 28-29 1.3375424502628874 1101 30-31 1.2761063700855573 1101 32-33 1.3391502510176352 1101 34-35 1.329905853506908 1101 36-37 1.368613801468392 1101 38-39 1.3390713723276022 1101 40-41 1.3365415695702794 1101 42-43 1.3265346490687975 1101 44-45 1.3440149825368692 1101 46-47 1.5128023469931264 1101 48-49 1.498795407348993 1101 50-51 1.2833323045420109 1101 52-53 1.3107794132677704 1101 54-55 1.3861876919906244 1101 56-57 1.4830911939421014 1101 58-59 1.5439531237188575 1101 60-61 1.498830625785697 1101 62-63 1.661068971156027 1101 64-65 1.496395641639534 1101 66-67 1.4528227297945442 1101 68-69 1.428741122020842 1101 70-71 1.4256390734447884 1101 72-73 1.343585056552648 1101 74-75 1.4204673530979157 1101 76-77 1.4117449772617086 1101 78-79 1.3630425058184805 1101 80-81 1.484820801111308 1101 82-83 1.4005005286939642 1101 84-85 1.5296698798165949 1101 86-87 1.3993379931079701 1101 88-89 1.2378331251549142 1101 90-91 1.1428978786919828 1101 92-93 1.0919537980564904 1101 94-95 1.0564240315649123 1101 96-97 0.9634414033428982 1101 98-99 0.8703951822783118 1101 100-101 0.7109873240784204 1102 1 0.7783855615217412 1102 2 0.8282056511714266 1102 3 0.7618777370520036 1102 4 0.8022000545824426 1102 5 0.8384429247588372 1102 6 0.8633878604460605 1102 7 0.8083745675626801 1102 8 0.8455873943513126 1102 9 0.9920909940627851 1102 10-11 1.005062095383387 1102 12-13 1.0320398260570869 1102 14-15 1.1997489884829236 1102 16-17 1.2358421826795194 1102 18-19 1.2102626492062498 1102 20-21 1.2374204076887807 1102 22-23 1.4638921408569416 1102 24-25 1.340817502317094 1102 26-27 1.2299140520932 1102 28-29 1.1125336898353027 1102 30-31 1.1360357149409808 1102 32-33 1.1742059040460688 1102 34-35 1.2377268808350905 1102 36-37 1.2840647872454838 1102 38-39 1.2354674860973276 1102 40-41 1.2421856902851758 1102 42-43 1.2432590788346616 1102 44-45 1.2745828906921375 1102 46-47 1.422478620202675 1102 48-49 1.3389252113302064 1102 50-51 1.211220780283508 1102 52-53 1.2562981505485773 1102 54-55 1.3150502388496115 1102 56-57 1.3596862332173174 1102 58-59 1.3747977621244871 1102 60-61 1.3307385234167235 1102 62-63 1.5506137906997353 1102 64-65 1.3372225188851026 1102 66-67 1.2566880896492307 1102 68-69 1.2855175377995671 1102 70-71 1.2563463170318343 1102 72-73 1.2315999438852323 1102 74-75 1.3599702639847386 1102 76-77 1.369191287896225 1102 78-79 1.3333501546640996 1102 80-81 1.4698302567994261 1102 82-83 1.4512532803614384 1102 84-85 1.6466733978042356 1102 86-87 1.5631427935086428 1102 88-89 1.448373015037351 1102 90-91 1.413715030870442 1102 92-93 1.391287870308279 1102 94-95 1.520961324326322 1102 96-97 1.5761818927588394 1102 98-99 1.6306835885530084 1102 100-101 1.6566095600076522 1103 1 0.5799579231099088 1103 2 0.6611722594063885 1103 3 0.63851737847056 1103 4 0.6834079000940605 1103 5 0.6661840617034969 1103 6 0.7018660657344924 1103 7 0.6110507040315696 1103 8 0.575394638160482 1103 9 0.6913459346735422 1103 10-11 0.6818713820950535 1103 12-13 0.7765003591982875 1103 14-15 0.8488744025530295 1103 16-17 0.8239513043306985 1103 18-19 0.8666437931222006 1103 20-21 0.8703359765034762 1103 22-23 1.0582442378924668 1103 24-25 0.9798957346107642 1103 26-27 0.9178760504044376 1103 28-29 0.9481784139028804 1103 30-31 0.9038369251945824 1103 32-33 0.8966862868416641 1103 34-35 0.8823825550887108 1103 36-37 0.9594491139100114 1103 38-39 0.9126235254163788 1103 40-41 0.9068593630191728 1103 42-43 0.8954127560961709 1103 44-45 0.9182962903981249 1103 46-47 1.0921069849163416 1103 48-49 1.0520316321823557 1103 50-51 0.9450957140611784 1103 52-53 0.9223137497505043 1103 54-55 0.9797994840905098 1103 56-57 1.1092028004770285 1103 58-59 1.1034746060106784 1103 60-61 1.0752937741932236 1103 62-63 1.2810854224950958 1103 64-65 1.1290938221282083 1103 66-67 1.122270837132195 1103 68-69 1.0320008073583686 1103 70-71 1.0705547187158686 1103 72-73 1.0567110493760303 1103 74-75 1.19773161766825 1103 76-77 1.2028098343178364 1103 78-79 1.1871703569834686 1103 80-81 1.308827002961916 1103 82-83 1.2736433878944915 1103 84-85 1.4329213436401744 1103 86-87 1.3203790093584686 1103 88-89 1.274070488620648 1103 90-91 1.233426276736438 1103 92-93 1.2537076554403477 1103 94-95 1.349424133926771 1103 96-97 1.452140851052448 1103 98-99 1.5293386078034317 1103 100-101 1.573010982507519 1104 1 0.39600059185726977 1104 2 0.5066420700315994 1104 3 0.5173953253204289 1104 4 0.5692985381973941 1104 5 0.5163781251646711 1104 6 0.538539226067499 1104 7 0.48192086190777417 1104 8 0.48587260889474493 1104 9 0.615043051409792 1104 10-11 0.581346234998513 1104 12-13 0.6012060080330812 1104 14-15 0.6693383289826258 1104 16-17 0.650275830553035 1104 18-19 0.6047244541497534 1104 20-21 0.6524515500101202 1104 22-23 0.8101655716416829 1104 24-25 0.74592150668812 1104 26-27 0.5915081825767601 1104 28-29 0.6366447846781824 1104 30-31 0.6295080783154461 1104 32-33 0.6202724262254264 1104 34-35 0.5888821550635797 1104 36-37 0.6444277714264288 1104 38-39 0.6186607098636685 1104 40-41 0.5865947675381662 1104 42-43 0.5958290442140282 1104 44-45 0.6346908309553712 1104 46-47 0.7522723037400922 1104 48-49 0.7505185322187629 1104 50-51 0.5696276378474217 1104 52-53 0.577410035410761 1104 54-55 0.6511215403255015 1104 56-57 0.7691210529373009 1104 58-59 0.7629274649852036 1104 60-61 0.6977600162004158 1104 62-63 0.8828258744719193 1104 64-65 0.7106783348787964 1104 66-67 0.7298180188008203 1104 68-69 0.6827601651005715 1104 70-71 0.7096729710242329 1104 72-73 0.7175189338208554 1104 74-75 0.8308904029660056 1104 76-77 0.8501208846352206 1104 78-79 0.7862094968081976 1104 80-81 0.8800242785761974 1104 82-83 0.8949907732911591 1104 84-85 1.10433353362507 1104 86-87 1.0064776302732916 1104 88-89 0.8627662739529107 1104 90-91 0.8359242748277964 1104 92-93 0.8379954141772998 1104 94-95 0.9332517142404626 1104 96-97 0.9329522123931895 1104 98-99 1.0242415166626557 1104 100-101 1.005569190933194 1105 1 0.20310956505998234 1105 2 0.3240029638895301 1105 3 0.28407900203812275 1105 4 0.3426250868695675 1105 5 0.3042282152194957 1105 6 0.29809508193184797 1105 7 0.25351893818838533 1105 8 0.24671549319405273 1105 9 0.3263520280399632 1105 10-11 0.3093453640067736 1105 12-13 0.3167214895097956 1105 14-15 0.3054868411606648 1105 16-17 0.2522365281322223 1105 18-19 0.2854535488993335 1105 20-21 0.2865467127075121 1105 22-23 0.4765073706643719 1105 24-25 0.39531104799772265 1105 26-27 0.34041897516339503 1105 28-29 0.3229349758617772 1105 30-31 0.27830400804219835 1105 32-33 0.27182575284708577 1105 34-35 0.3018814374842478 1105 36-37 0.3360485552269523 1105 38-39 0.32859895481141876 1105 40-41 0.333830294016785 1105 42-43 0.24544857474369763 1105 44-45 0.3343486897652923 1105 46-47 0.4510008747062386 1105 48-49 0.36380674184482586 1105 50-51 0.22953557932676105 1105 52-53 0.24511932065793118 1105 54-55 0.2293622684204344 1105 56-57 0.33515766340482855 1105 58-59 0.31838757049819577 1105 60-61 0.316446240784213 1105 62-63 0.4785368318410903 1105 64-65 0.32182137308606684 1105 66-67 0.29463106173125553 1105 68-69 0.3139672176239827 1105 70-71 0.3285551008356613 1105 72-73 0.3127013395120066 1105 74-75 0.46549328517902566 1105 76-77 0.45231114911735304 1105 78-79 0.40997183709247764 1105 80-81 0.5745036213082244 1105 82-83 0.48726074694314647 1105 84-85 0.6625397577815875 1105 86-87 0.5625209843937675 1105 88-89 0.45814545490553016 1105 90-91 0.43003005165022046 1105 92-93 0.44235780850609174 1105 94-95 0.5012848963417511 1105 96-97 0.5546079825621177 1105 98-99 0.528418475980974 1105 100-101 0.530942062275745 1106 1 -0.14522643142494474 1106 2 -0.010463141581755764 1106 3 -0.05981874584789182 1106 4 -0.008214193875751619 1106 5 -0.0365271726761236 1106 6 -0.039409348012441114 1106 7 -0.06563456089605069 1106 8 -0.07287048216752368 1106 9 -0.07722194939425009 1106 10-11 -0.12570331828937498 1106 12-13 -0.15560477039978338 1106 14-15 -0.23767737271285938 1106 16-17 -0.15372767589649783 1106 18-19 -0.1618811587831388 1106 20-21 -0.1634938199207383 1106 22-23 -0.06359523311617465 1106 24-25 -0.13697356920457082 1106 26-27 -0.2912474764371993 1106 28-29 -0.08978902211212159 1106 30-31 -0.10765093983471274 1106 32-33 -0.16176114462138713 1106 34-35 -0.17991036793658566 1106 36-37 -0.13482702416401082 1106 38-39 -0.20051281503729257 1106 40-41 -0.21728606391848615 1106 42-43 -0.22579412899830942 1106 44-45 -0.23152300162198713 1106 46-47 -0.11533235268985464 1106 48-49 -0.15786669206366355 1106 50-51 -0.40154715862670187 1106 52-53 -0.4251574432959586 1106 54-55 -0.5315245600801539 1106 56-57 -0.38645941058124933 1106 58-59 -0.36742004177696685 1106 60-61 -0.4398705948946251 1106 62-63 -0.2876292956320228 1106 64-65 -0.4015406493622109 1106 66-67 -0.4036445395345005 1106 68-69 -0.4138073323503768 1106 70-71 -0.4775757656529507 1106 72-73 -0.5239806290028035 1106 74-75 -0.342351522123554 1106 76-77 -0.29632552513778876 1106 78-79 -0.2572006538183089 1106 80-81 -0.18544458314828205 1106 82-83 -0.1750058957526619 1106 84-85 0.03826007869262327 1106 86-87 -0.09634496387465319 1106 88-89 -0.33950343842832 1106 90-91 -0.3967538996272424 1106 92-93 -0.43508226976789643 1106 94-95 -0.4029235621814635 1106 96-97 -0.35907717306299247 1106 98-99 -0.27849887296701326 1106 100-101 -0.3077124931660542 1107 1 -0.7972311605923146 1107 2 -0.6058884235649842 1107 3 -0.9464659963519821 1107 4 -0.84336614279853 1107 5 -0.8748230311744365 1107 6 -0.7265403099202601 1107 7 -0.600852986298456 1107 8 -0.6194459310340434 1107 9 -0.7794980223502961 1107 10-11 -0.825299190609762 1107 12-13 -0.8178261581561017 1107 14-15 -0.9985784564704971 1107 16-17 -1.0577684543859505 1107 18-19 -1.1401682207849504 1107 20-21 -1.1357799845751018 1107 22-23 -1.0493550914309822 1107 24-25 -1.2363798922018034 1107 26-27 -1.2014294750304657 1107 28-29 -1.2681942816596887 1107 30-31 -1.2041658523532206 1107 32-33 -1.2424825611489894 1107 34-35 -1.2490838363901133 1107 36-37 -1.1556293275271656 1107 38-39 -1.237694108882586 1107 40-41 -1.270210253878517 1107 42-43 -1.3933847550593121 1107 44-45 -1.3099475646199075 1107 46-47 -1.1547459159640212 1107 48-49 -1.468705571530208 1107 50-51 -1.6114771455540655 1107 52-53 -1.5059245448538228 1107 54-55 -1.454357125882833 1107 56-57 -1.3138554142805816 1107 58-59 -1.392292003663627 1107 60-61 -1.3827571310642703 1107 62-63 -1.2220218766370046 1107 64-65 -1.367418625137784 1107 66-67 -1.444476715590195 1107 68-69 -1.4342568405395077 1107 70-71 -1.4244673119795515 1107 72-73 -1.4471876593977306 1107 74-75 -1.3568976992316237 1107 76-77 -1.404704366523422 1107 78-79 -1.6744368009429529 1107 80-81 -1.5192645613691163 1107 82-83 -1.547529932621817 1107 84-85 -1.3842347835087807 1107 86-87 -1.4450428253284038 1107 88-89 -1.546469790074223 1107 90-91 -1.7025168792795213 1107 92-93 -1.920093572628197 1107 94-95 -1.838422434367196 1107 96-97 -1.9091247949637271 1107 98-99 -1.9728234697192804 1107 100-101 -2.0248609778689293 1201 1 0.931067333173285 1201 2 0.9484085646095828 1201 3 0.9403191751086197 1201 4 0.9705666126424219 1201 5 0.9607915905293396 1201 6 0.9858098652513618 1201 7 0.9007235871086365 1201 8 0.927632160753312 1201 9 1.0419039760298006 1201 10-11 1.0689885330484614 1201 12-13 1.1020425516497525 1201 14-15 1.2182542435967392 1201 16-17 1.3126428442623634 1201 18-19 1.2175938408502063 1201 20-21 1.2561928458491494 1201 22-23 1.4048670206065879 1201 24-25 1.315593400241319 1201 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56-57 -3.964858571466273 2307 58-59 -3.988711969146607 2307 60-61 -4.027173947042556 2307 62-63 -3.8664692935961256 2307 64-65 -4.136545259021474 2307 66-67 -4.041874528703083 2307 68-69 -4.028041492280369 2307 70-71 -3.9798288049628425 2307 72-73 -3.999393038257029 2307 74-75 -3.882359449617237 2307 76-77 -3.891255183214522 2307 78-79 -3.93383397852217 2307 80-81 -3.711226801984118 2307 82-83 -3.7426818511514846 2307 84-85 -3.4839071854582286 2307 86-87 -3.5234053056837986 2307 88-89 -3.6803888534982256 2307 90-91 -3.7124999797375935 2307 92-93 -3.680919821639062 2307 94-95 -3.7750921778155373 2307 96-97 -3.782171890007575 2307 98-99 -3.750898270519034 2307 100-101 -3.569794056509881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1216675.0 3 313406.0 4 295531.0 5 333796.0 6 442673.0 7 666968.0 8 1028569.0 9 1573344.0 10 1944803.0 11 1701363.0 12 1115806.0 13 815468.0 14 665211.0 15 585606.0 16 544167.0 17 531905.0 18 546949.0 19 540232.0 20 572852.0 21 603953.0 22 651885.0 23 712802.0 24 794718.0 25 823834.0 26 881116.0 27 982315.0 28 1106811.0 29 1269509.0 30 1476613.0 31 1745102.0 32 2117992.0 33 2627614.0 34 3389021.0 35 4612138.0 36 6911148.0 37 1.209967E7 38 1.7138128E7 39 6217213.0 40 10207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.84728734319234 16.821990629254483 8.526336561052016 40.80438546650116 2 15.207206999798567 15.982071374663834 55.15092027708023 13.659801348457371 3 18.711471090300346 20.89420057575539 38.079483691276096 22.31484464266817 4 20.126216738557893 25.66285814101386 36.378155883096824 17.83276923733142 5 19.273689849115797 29.101757574786596 38.13009114860083 13.494461427496773 6 15.046234914889869 33.92911509895292 37.753388413923325 13.271261572233882 7 12.09353341163415 19.285655172031145 55.228770637650335 13.392040778684363 8 12.43475436045309 21.94384404593419 49.42860077939123 16.192800814221485 9 13.47381965653534 22.194761572845938 47.629464038725146 16.70195473189358 10-11 16.038048024326525 31.138472747343997 38.50460663669862 14.318872591630855 12-13 15.92679657152884 25.393051849038486 43.35388723313737 15.326264346295304 14-15 16.23851926720428 27.669733060695627 41.71643784046925 14.375309831630847 16-17 17.774765420703325 27.529548492742258 39.77350822067939 14.92217786587503 18-19 17.921180422004227 27.994901605471 39.06132593548545 15.022592037039326 20-21 18.15072646040365 28.532432210926174 37.637964963960414 15.67887636470976 22-23 18.375019313660445 28.83795449570701 36.88710277910591 15.899923411526634 24-25 17.587443038315715 29.895055067565433 36.01783425831114 16.499667635807704 26-27 17.24337409728256 30.896065756092383 35.509196219572964 16.351363927052095 28-29 17.764691608281748 30.696271557142236 34.642581305807404 16.896455528768612 30-31 18.000893551223793 30.91951951113847 33.94273636531634 17.13685057232139 32-33 17.71837834741951 30.686250190600717 34.069034587113414 17.52633687486636 34-35 18.083530742435055 31.838054264250754 32.96315295087865 17.115262042435543 36-37 18.248069163825942 31.68108153249673 33.2874994984063 16.783349805271033 38-39 18.245161885787486 31.083951550556822 33.6144475255877 17.056439038067992 40-41 18.546661939256452 30.86591571607058 33.660366893131936 16.927055451541033 42-43 18.221440555955365 31.12970669700385 33.88793025626516 16.760922490775627 44-45 18.339843309117374 30.153149786356398 34.11711326420029 17.389893640325937 46-47 17.744819250667994 31.14050241527201 33.57548536124269 17.539192972817307 48-49 17.31691906064047 31.293638621033264 33.440073373424234 17.94936894490203 50-51 17.418615715869905 31.322637094457395 33.750103564176456 17.50864362549625 52-53 17.693736390293278 31.443261389408473 33.345692629231365 17.517309591066883 54-55 18.22267084837492 31.841368771626826 32.236584747222096 17.699375632776157 56-57 18.61457101172051 32.062235187758695 32.21171541750289 17.11147838301791 58-59 18.144725938289227 32.65037080775055 32.20724093008902 16.997662323871204 60-61 18.209115546092164 33.37057273426643 30.88067886925494 17.53963285038646 62-63 18.28634472800056 33.57153664587364 31.068948865759026 17.073169760366767 64-65 18.179652706481736 32.556017270505194 31.84234292627619 17.421987096736878 66-67 18.09565450997488 33.4404151680192 30.89354595211996 17.570384369885954 68-69 18.019574096287798 32.771857652422796 31.543565905964705 17.665002345324705 70-71 17.586612376255168 32.7005582998448 31.636571102857246 18.07625822104278 72-73 17.80070170522424 33.06442530699458 31.236536618660253 17.898336369120923 74-75 18.30787572325875 32.34394923221813 31.360161462556313 17.988013581966808 76-77 17.956428335184675 32.982354817010815 31.020469998424566 18.04074684937994 78-79 18.047969227159225 33.44033644474582 30.989287367537138 17.52240696055782 80-81 18.317374258685852 33.591319722089835 30.71568960701585 17.375616412208466 82-83 18.312822805041517 33.305165445566 30.975985512439337 17.406026236953142 84-85 18.153510698389265 33.47843697055946 30.570108232467025 17.79794409858425 86-87 17.86403914051404 33.62876533395916 30.936956174795764 17.570239350731036 88-89 18.200628714432685 33.47234005854142 30.718202148807546 17.608829078218356 90-91 17.645958028799946 34.27984924690604 30.51793982502638 17.55625289926763 92-93 18.325415120441313 33.241915704130435 30.768657287595087 17.664011887833166 94-95 18.337543251992003 34.03499141272419 29.984203760165194 17.643261575118615 96-97 18.448283423529517 34.065635920731566 30.246833385712346 17.239247270026574 98-99 18.954914522363232 33.625136256566776 29.867255628666317 17.552693592403678 100-101 19.21129228036777 34.48065121480281 29.11513301542722 17.192923489402204 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28675.0 1 36633.0 2 45359.0 3 55319.0 4 76970.5 5 105173.0 6 134734.5 7 165215.5 8 199754.5 9 234527.0 10 268580.0 11 301103.0 12 333279.5 13 365167.0 14 396940.0 15 429065.0 16 459551.0 17 494827.0 18 540177.5 19 585460.0 20 639136.5 21 716502.5 22 819364.0 23 935022.0 24 1112780.0 25 1378810.5 26 1610460.5 27 1853068.0 28 2093748.5 29 2319193.0 30 2619291.5 31 2936991.5 32 3226230.0 33 3613387.5 34 3905072.5 35 3996331.5 36 3946448.5 37 3805601.5 38 3810457.0 39 3676236.0 40 3440407.5 41 3242857.5 42 2807346.0 43 2363552.0 44 2078917.5 45 1855035.0 46 1682425.5 47 1519910.5 48 1397292.0 49 1268330.0 50 1076761.5 51 874711.5 52 722009.5 53 598020.5 54 485125.5 55 401640.5 56 341677.5 57 286958.5 58 235605.5 59 187921.5 60 138085.0 61 95933.5 62 71164.5 63 53881.0 64 39108.5 65 26695.0 66 17479.0 67 11888.0 68 7934.0 69 5096.0 70 3508.0 71 2688.0 72 2160.0 73 1726.0 74 1409.0 75 1079.0 76 854.0 77 736.5 78 646.0 79 526.0 80 413.5 81 306.0 82 236.0 83 195.5 84 139.0 85 96.0 86 85.5 87 71.5 88 49.5 89 35.5 90 19.5 91 15.0 92 13.5 93 9.0 94 6.0 95 4.5 96 2.0 97 2.0 98 2.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12128477085079582 2 0.14840740659456977 3 0.12926324204116865 4 0.11168389206465373 5 0.11016074052270419 6 0.19209477487581259 7 0.17376794103719856 8 0.1828014673181736 9 0.24274477152500173 10-11 0.2305779399401128 12-13 0.23476139880110697 14-15 0.26237418789707706 16-17 0.291885708541117 18-19 0.31179206155718314 20-21 0.3035139841302804 22-23 0.3018192789150622 24-25 0.3015202853702226 26-27 0.27484920340215935 28-29 0.25106451198684115 30-31 0.24428875458441962 32-33 0.27724789136946926 34-35 0.2945080289753664 36-37 0.2958786202864449 38-39 0.2933665843564396 40-41 0.30162321757418376 42-43 0.28422399895460093 44-45 0.30578155607587787 46-47 0.3078971804822945 48-49 0.08739985692178573 50-51 0.018617249699790263 52-53 0.008902410259262571 54-55 0.005998864339190629 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.73960859269706E-6 64-65 4.901533521961499E-6 66-67 6.923416099770617E-5 68-69 9.312913691726847E-5 70-71 8.455145325383586E-5 72-73 4.147922742959918E-4 74-75 9.925605381972035E-5 76-77 0.0013154490589564172 78-79 7.444204036479026E-4 80-81 1.157987294563404E-4 82-83 0.0 84-85 4.717726014887942E-5 86-87 1.102845042441337E-5 88-89 3.3085351273240117E-4 90-91 3.6393886400564124E-4 92-93 3.8599576485446804E-4 94-95 4.105034324642755E-5 96-97 0.0 98-99 4.533918507814386E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8.1607113E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.8334548841745 #Duplication Level Percentage of deduplicated Percentage of total 1 80.14598389893844 21.505936430999405 2 8.33706853997712 4.474247050674932 3 3.2991833584817156 2.655854634133153 4 1.7610658919860387 1.8902192864266358 5 1.1616425904891958 1.5585442021713714 6 0.7869411899497485 1.2669810550209102 7 0.5608066224850384 1.053386544223899 8 0.4331417183166936 0.9298151005523854 9 0.343277601960121 0.8290191640450066 >10 2.5198262442478394 13.482003022025433 >50 0.3321970088354749 6.250027684300196 >100 0.26520536239623177 14.292496336383689 >500 0.03044085504523347 5.639159258348692 >1k 0.0198194181333657 10.327054173206001 >5k 0.001882508311441158 3.5080995937901696 >10k+ 0.0015171904461961096 10.337156463697678 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT 220990 0.2707974732545679 No Hit GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT 212349 0.26020893546375057 No Hit GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT 199797 0.2448279232718354 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 175001 0.21444331696919605 Illumina Single End PCR Primer 1 (100% over 50bp) ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 143497 0.17583883895022728 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123653 0.1515223311477763 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 107048 0.13117484011473363 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 104629 0.1282106377173274 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 103750 0.12713352572587638 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 84602 0.10366988475624668 No Hit CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC 83875 0.10277903103863018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.29728290964048687 0.0 0.0 1.7155367326865245E-5 0.0 2 0.29792991206538577 0.0 0.0 2.328228422931712E-5 0.0 3 0.2980034350682152 0.0 0.0 3.3085351273240116E-5 0.0 4 0.33316826193814747 0.0 0.0 4.0437651556182365E-5 0.0 5 0.33413631480873485 0.0 0.0 4.5339185078143865E-5 0.0 6 0.33485561485308274 0.0 1.2253833804903747E-6 5.269148536108611E-5 0.0 7 0.3381494453798409 0.0 1.2253833804903747E-6 7.107223606844173E-5 0.0 8 0.3785527862013695 0.0 1.2253833804903747E-6 7.22976194489321E-5 0.0 9 0.3789154996819946 2.4507667609807494E-6 1.2253833804903747E-6 8.332606987334548E-5 0.0 10-11 0.38787121411830855 2.4507667609807494E-6 1.2253833804903747E-6 8.638952832457142E-5 0.0 12-13 0.39148915854920635 2.4507667609807494E-6 1.2253833804903747E-6 1.0293220396119147E-4 0.0 14-15 0.3935631199206863 2.4507667609807494E-6 1.2253833804903747E-6 1.1763680452707596E-4 0.0 16-17 0.3952994881708412 4.288841831716311E-6 1.2253833804903747E-6 1.2927794664173453E-4 0.0 18-19 0.39683121739645416 6.126916902451873E-6 1.2253833804903747E-6 1.543983059417872E-4 0.0 20-21 0.39862946750732375 7.352300282942248E-6 1.2253833804903747E-6 1.7706789848085915E-4 0.0 22-23 0.40060846166681574 7.964991973187435E-6 1.2253833804903747E-6 1.8993442397600808E-4 0.0 24-25 0.40243795905388785 9.19037535367781E-6 1.2253833804903747E-6 2.119913248248348E-4 0.0 26-27 0.4045958591869314 1.0415758734168185E-5 1.2253833804903747E-6 2.91028552866464E-4 0.0 28-29 0.4069155099261997 1.1028450424413373E-5 2.4507667609807494E-6 3.4188196315681455E-4 0.0 30-31 0.4095562111111565 1.5317292256129683E-5 2.4507667609807494E-6 3.6026271386417017E-4 0.0 32-33 0.41226247030696944 1.8993442397600808E-5 2.4507667609807494E-6 3.682277058373576E-4 0.0 34-35 0.41533144298340763 1.9606134087845995E-5 2.4507667609807494E-6 3.9089729837642954E-4 0.0 36-37 0.4184690371291532 2.0218825778091183E-5 3.676150141471124E-6 4.123415075350111E-4 0.0 38-39 0.4221335461285097 2.083151746833637E-5 3.676150141471124E-6 4.1663034936672736E-4 0.0 40-41 0.42624654544512564 2.083151746833637E-5 3.676150141471124E-6 4.2643341641065036E-4 0.0 42-43 0.43069897595813733 2.328228422931712E-5 3.676150141471124E-6 4.631949178253616E-4 0.0 44-45 0.43613722740075367 2.328228422931712E-5 3.676150141471124E-6 4.956675774083566E-4 0.0 46-47 0.45385872184940546 2.328228422931712E-5 3.676150141471124E-6 5.361052289645389E-4 0.0 48-49 0.46134520161251136 2.3894975919562304E-5 3.676150141471124E-6 5.587748215036109E-4 0.0 50-51 0.46860008391670466 2.512035930005268E-5 3.676150141471124E-6 5.679651968572886E-4 0.0 52-53 0.4766851634611802 2.757112606103343E-5 3.676150141471124E-6 5.802190306621924E-4 0.0 54-55 0.48649497011369586 2.8183817751278616E-5 3.676150141471124E-6 5.973743979890576E-4 0.0 56-57 0.49641996280397765 3.0634584512259366E-5 3.676150141471124E-6 6.059520816524902E-4 1.2253833804903747E-6 58-59 0.507733314864355 3.1247276202504553E-5 3.676150141471124E-6 6.243328323598459E-4 1.2253833804903747E-6 60-61 0.5206304749440163 3.431073465373049E-5 3.676150141471124E-6 6.316851326427881E-4 1.2253833804903747E-6 62-63 0.5354698676817546 3.5536118034220866E-5 3.676150141471124E-6 6.604816420843119E-4 1.838075070735562E-6 64-65 0.552010705243304 4.656456845863424E-5 3.676150141471124E-6 6.794750844819127E-4 2.4507667609807494E-6 66-67 0.5704049351678451 5.024071860010536E-5 3.676150141471124E-6 6.898908432160809E-4 2.4507667609807494E-6 68-69 0.5920133456994123 5.207879367084092E-5 3.676150141471124E-6 6.947923767380424E-4 2.4507667609807494E-6 70-71 0.6177598759068955 5.452956043182167E-5 3.676150141471124E-6 7.009192936404942E-4 2.4507667609807494E-6 72-73 0.6472934534517842 5.514225212206686E-5 4.901533521961499E-6 7.088842856136817E-4 2.4507667609807494E-6 74-75 0.6817255010601833 5.514225212206686E-5 4.901533521961499E-6 7.309411864625086E-4 2.4507667609807494E-6 76-77 0.7234638970747562 5.514225212206686E-5 4.901533521961499E-6 8.259083984505126E-4 2.4507667609807494E-6 78-79 0.7727610704718839 5.698032719280242E-5 4.901533521961499E-6 8.540922162017912E-4 2.4507667609807494E-6 80-81 0.8320812672297329 5.943109395378317E-5 4.901533521961499E-6 8.785998838115986E-4 2.4507667609807494E-6 82-83 0.9027417744823297 6.004378564402836E-5 4.901533521961499E-6 8.982060178994446E-4 2.4507667609807494E-6 84-85 0.990879924890861 6.0656477334273545E-5 4.901533521961499E-6 9.129106184653291E-4 2.4507667609807494E-6 86-87 1.098608892094982 6.126916902451873E-5 4.901533521961499E-6 9.233263771994973E-4 2.4507667609807494E-6 88-89 1.2294136664288073 6.31072440952543E-5 4.901533521961499E-6 9.28840602411704E-4 3.676150141471124E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 73165 0.0 68.40008 1 GCGTGTT 99205 0.0 50.485 2 GTTTTTT 1535990 0.0 43.259804 1 ACCACCG 7975 0.0 42.31158 1 CCACCGA 8645 0.0 39.582684 2 CCCGAAA 22860 0.0 35.966778 1 CGCCGTA 44730 0.0 34.11328 46-47 CCGAAAC 24065 0.0 33.613323 2 CGTGTTT 151460 0.0 33.461243 3 GTCGCCG 47605 0.0 32.79502 44-45 CGGCAAC 6490 0.0 32.367504 1 CCGTATC 47420 0.0 32.3269 48-49 ACCGAGA 10515 0.0 32.24064 4 TCGTCTA 19470 0.0 31.494799 44-45 CGGCCTT 7505 0.0 31.092985 1 CGAAACC 26670 0.0 30.5007 3 CGAGCTA 20855 0.0 30.209398 12-13 CCCAGAT 40750 0.0 29.891838 1 GTGTTTT 363680 0.0 29.47247 1 TCGGTGG 56310 0.0 28.916786 38-39 >>END_MODULE